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Wyszukujesz frazę "Szczerba, A." wg kryterium: Autor


Tytuł:
Analiza porównawcza właściwości przeciwbakteryjnych preparatów stosowanych do dezynfekcji w pomieszczeniach inwentarskich
Comparison of the antibacterial properties of preparations for disinfection of livestock facilities
Autorzy:
Chmiel, M. J.
Szczerba, A.
Powiązania:
https://bibliotekanauki.pl/articles/337763.pdf
Data publikacji:
2017
Wydawca:
Instytut Technologiczno-Przyrodniczy
Tematy:
mikroorganizmy
pomieszczenia inwentarskie
preparaty do dezynfekcji
disinfectants
livestock facilities
microorganisms
Opis:
Obecność drobnoustrojów chorobotwórczych w budynkach inwentarskich może stanowić istotny problem dla hodowców/rolników, dlatego dbając o dobrostan zwierząt hodowlanych/gospodarskich powinni oni regularnie przeprowadzać dezynfekcję pomieszczeń z zastosowaniem środków i metod umożliwiających osiągniecie zadowalających efektów. Praca miała na celu analizę porównawczą właściwości biobójczych preparatów zalecanych do dezynfekcji w pomieszczeniach inwentarskich na podstawie hamowania w warunkach in vitro wzrostu bakterii: Staphylococcus aureus ATCC29213, Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, Salmonella enterica subsp. enterica serowar Typhimurium ATCC14028, Enterobacter cloacae, Bacillus cereus, B. pseudomycoides i B. thuringiensis. Zbadano dziesięć preparatów pochodzących od różnych producentów, o różnym składzie, rekomendowanych jako dezynfekujące. Analizy wykonano metodą dyfuzyjną. Nie wszystkie przebadane preparaty wykazywały zadowalające działanie przeciwbakteryjne – jedynie trzy skutecznie hamowały rozwój wszystkich bakterii testowych w zalecanych dawkach, dlatego producenci powinni rzetelniej opisywać działanie oferowanych produktów i nie narażać hodowców/ rolników na nieskuteczną dezynfekcję pomieszczeń. Najlepsze właściwości przeciwbakteryjne wykazały dwie grupy preparatów: zawierające aktywne formy srebra – nanocząstki (nr 1) oraz czwartorzędowe związki amoniowe, glutaral i chlorki w różnych proporcjach (2 i 3). Bakterie Gramujemne były mniej wrażliwe od Gram-dodatnich na działanie badanych preparatów, bez względu na zawarte w nich substancje aktywne, co może stanowić istotny problem w przypadku salmonelloz, zatruć wywołanych przez Escherichia coli i zakażeń Pseudomons aeruginosa oraz innych chorób, których przyczyną są pałeczki Gram-ujemne.
The presence of pathogens in livestock buildings can be a major problem for farmers, because taking care of the welfare of the animals, they should carry out regular disinfection of the premises using preparations and methods to achieve satisfactory results. The work was aimed at comparative analysis of the biocidal properties of the preparations recommended for the disinfection in livestock facilities on the basis of in vitro inhibition of growth of bacteria: Staphylococcus aureus ATCC29213, Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, Salmonella enterica subsp. enterica ser. Typhimurium ATCC14028, Enterobacter cloacae, Bacillus cereus, B. pseudomycoides and B. thuringiensis. Studies were performed using five different in composition products from different manufacturers using diffusion method. Not all tested preparations had satisfactory antibacterial activity – only three effectively inhibited the growth of all tested bacteria at the recommended doses, so manufacturers should accurately describe the effects of the offered products and do not expose farmers to carry out ineffective disinfection of the buildings. Best antimicrobial properties revealed the two groups of tested preparations: containing the active forms of silver – nanosilver (no. 1) and quaternary ammonium compounds, glutaral and chloride in various proportions (no. 2 and no. 6). Gram-negative bacteria were less sensitive than the Gram-positive on tested chemicals regardless of the active agents which can be a significant problem in the case of salmonellosis, Escherichia coli intoxications and Pseudomons aeruginosa infections and other diseases that are caused by Gram-negative bacteria.
Źródło:
Woda-Środowisko-Obszary Wiejskie; 2017, 17, 2; 37-49
1642-8145
Pojawia się w:
Woda-Środowisko-Obszary Wiejskie
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Plasma surface modifications of polymeric substrate for applications in biomedicine
Autorzy:
Kyzioł, A.
Szczerba, A.
Klich, M.
Kaczmarek, Ł.
Kyzioł, K.
Powiązania:
https://bibliotekanauki.pl/articles/283990.pdf
Data publikacji:
2016
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
polymeric substrate
biomedicine
modification
Źródło:
Engineering of Biomaterials; 2016, 19, 138; 107
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of an aptamer and a reagent based on gold nanoparticles for detection of Escherichia coli
Autorzy:
Pięta, P. P.
Szczerba, A.
Dobroczyńska, J. I.
Grabas, K.
Powiązania:
https://bibliotekanauki.pl/articles/207434.pdf
Data publikacji:
2013
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
Escherichia coli
złoto
aptamery
komórki bakteryjne
nanocząstki złota
zawiesiny wodne
gold
aptamers
bacterial cells
gold nanoparticles
water suspensions
Opis:
Plates functionalized with an Escherichia coll binding aptamer and a reagent based on gold nanoparticles were used for the detection of bacterial cells. Bacteria suspended in samples were caught by specific aptamers immobilized on a plastic plate whereas the gold nanoparticle reagent is an indicator reagent for visualization of the test result. Changes in colour of the gold nanoparticle reagent caused by gold nanoparticle agglomeration were observed with the naked eye. The test was designed for the detection of Escherichia coli in water suspensions.
Źródło:
Environment Protection Engineering; 2013, 39, 4; 125-133
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A comparison of the effectiveness of the microscopic method and the multiplex PCR method in identifying and discriminating the species of Nosema spp. spores in worker bees (Apis mellifera)from winter hive debris
Autorzy:
Michalczyk, M.
Sokol, R.
Szczerba-Turek, A.
Bancerz-Kisiel, A.
Powiązania:
https://bibliotekanauki.pl/articles/32256.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The objective of this study was to compare the effectiveness of the multiplex PCR method and traditional light microscopy in identifying and discriminating the species of Nosema spp. spores in worker bees from winter hive debris in the Province of Warmia and Mazury (NE Poland). A total of 1000 beesdead after from the bottom of the hive from bee colonies were analyzed. Spores were identified with the use of a light microscope (400-600x magnification). Spores were assigned to species by the multiplex PCR method. The microscopic evaluation revealed the presence of Nosema spp. spores in 803 samples (80.3%). Nosema ceranae spores were observed in 353 positive samples (43.96%), Nosema apis spores were found in 300 samples (37.35%), while 150 samples (19.67%) showed signs of a mixed infection. A multiplex PCR analysis revealed that 806 samples were infested with Nosema spp., of which 206 were affected only by Nosema ceranae, 600 showed signs of mixed invasion, while no samples were infected solely by Nosema apis parasites. In two cases, the presence of spores detected under a light microscope was not confirmed by the PCR analysis. The results of the study indicate that Nosema ceranae is the predominant parasitic species found in post-winter worker bees from the bottom of the hive in the region of Warmia and Mazury.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sequence variants of Yersinia enterocolitica ystB gene detected in wild animals in Poland
Autorzy:
Pieczywek, M.
Bancerz-Kisiel, A.
Szczerba-Turek, A.
Szweda, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087747.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Yersinia enterocolitica
ystB gene
phylogenetic analysis
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 397-399
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distribution of the ymoA and ystA genes and enterotoxins Yst production by Yersinia enterocolitica strains isolated from humans and pigs
Autorzy:
Bancerz-Kisiel, A.
Szczerba-Turek, A.
Platt-Samoraj, A.
Szweda, W.
Powiązania:
https://bibliotekanauki.pl/articles/30931.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Yersinia (Y.) enterocolitica is the third etiological agent of human diarrhea in terms of the number of confirmed clinical cases. One of the important virulence markers is the yst gene which encodes the production of enterotoxins Yst (Yersinia stable toxins). However, not all strains with yst genes produce enterotoxins, what seems to be caused by the ymoA gene encoding the production of the YmoA protein inhibiting the expression of various genes. The purpose of our study was to evaluate the distribution of the ymoA and ystA genes and Yst production by Y. enterocolitica isolated from humans and pigs. All the studied strains obtained from pigs had the ystA gene which indicates that they belong to the group of strains commonly regarded as pathogenic, but the ability to produce YstA was detected in only 14 out of 96 examined strains. The fragments of ystA gene were also detected in all Y.enterocolitica strains isolated from human cases of diarrhea. Amplification of a fragment of the ymoA gene was detected in all the studied strains, both from humans and pigs, based on the presence of a 330 bp band. Thus no correlation was identified between the occurrence of the ymoA and ystA genes and the production of a specific type of enterotoxin.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phylogenetic analysis of bovine papillomavirus E5 detected in equine sarcoids in Poland
Autorzy:
Szczerba-Turek, A.
Siemionek, J.
Bancerz-Kisiel, A.
Ras, A.
Szweda, W.
Powiązania:
https://bibliotekanauki.pl/articles/30859.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the study was to analyse a part of the sequence of the E5 gene of bovine papil-lomaviruses (BPV) associated with equine sarcoids in Polish horses. Samples of 40 skin lesions obtained from 29 horses were collected for molecular examination. The PCR amplicons of BPV DNA were detected in 38 specimens. After phylogenetic analysis 37 specimens were recognized as BPV-1 and one as BPV-2. Phylogenetic analysis has allowed the classification of the amplicons into two phylogenetic groups (A1,) and four separate isolates (2, 10, 16, 17).
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus)
Autorzy:
Bancerz-Kisiel, A.
Szczerba-Turek, A.
Platt-Samoraj, A.
Socha, P.
Szweda, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087938.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Free-living animals are an important environmental reservoir of pathogens dangerous for other animal species and humans. One of those is Yersinia (Y.) enterocolitica, the causative agent of yersiniosis - foodborne, enzootic disease, significant for public health. The purpose of the study was to identify bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus) obtained during the 2010/2011 hunting season in north-eastern Poland. From among 48 rectal swabs obtained from 24 roe deer, two strains of Y. enterocolitica from one animal were isolated. Although both belonged to biotype 1A they were identified as different serotypes. The strain obtained from cold culture (PSB) belonged to serotype O:5, while the strain isolated from warm culture (ITC) was regarded as nonidentified (NI), what may suggest mixed infection in that animal. The presence of ystB gene, coding for YstB enterotoxin, directly related to Y. enterocolitica pathogenicity was detected in both strains using triplex PCR. The effect of the examination of 32 swabs obtained from 16 red deer was the isolation of two Y. enterocolitica strains from two different animals. Both belonged to biotype 1A with NI serotype, but were originated from different types of culture. They gave positive results in case of products of a size corresponding to the ystB gene. No amplicons corresponding to ail and ystA genes were found. Roe deer and red deer may carry and shed Y. enterocolitica, what seems to be important in aspect of an environmental reservoir of this pathogen. The Y. enterocolitica strains isolated from wild ruminants had the amplicons of the ystB gene, what suggest they can be potential source of Y. enterocolitica infection for humans.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 2; 315-319
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Yersinia enterocolitica strains isolated from beavers (Castor fiber)
Autorzy:
Platt-Samoraj, A.
Syczylo, K.
Bancerz-Kisiel, A.
Szczerba-Turek, A.
Gizejewska, A.
Szweda, W.
Powiązania:
https://bibliotekanauki.pl/articles/31544.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Pseudocloacal swabs and palatine tonsils from beavers have been examined for the Yersinia enterocolitica presence. Thirty-six samples from 24 beavers were collected and subjected to bacteriological examinations including sero- and biotypisation. Amplicons confirmed by PCR as Y. enterocolitica were sequenced. Positive samples originated from 4 out of the 24 beavers (16.7 %) and all the strains belonged to biotype 1A. The study suggested that Y. enterocolitica could be isolated from beavers, which may therefore be treated as a reservoir, a significant factor of water contamination and a vector of the Y. enterocolitica.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Quantitative PCR High-Resolution Melting (qPCR-HRM) curve analysis, a new approach to rapid detection and differentiation of bovine papillomavirus detected in equine sarcoids
Autorzy:
Szczerba-Turek, A.
Bancerz-Kisiel, A.
Lipczynska, K.
Siemionek, J.
Ras, A.
Platt-Samoraj, A.
Szweda, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087954.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the study was to evaluate a novel diagnostic scheme which combines quantitative PCR and High-Resolution Melting (qPCR-HRM) curve analysis for rapid differentiation based on E5 partial CDS of bovine papillomavirus type 1 or 2 (BPV-1 or BPV-2), and to perform a phylogenetic analysis of the complete CDS of the E5 gene of BPV detected in equine sarcoids. Samples of 38 skin lesions obtained from 27 horses were collected for molecular examinations. All lesions were clinically diagnosed as sarcoids, but results of histopathological examinations did not always corroborate the clinical diagnosis. Although all the samples were positive for the presence of BPV DNA, after qPCR-HRM analysis 6 (16%) specimens were recognized as BPV-1 “wild”, 24 (63%) as BPV-1 “European” and 8 (21%) as a “variant” of BPV E5 ORF partial CDS. Phylogenetic analysis based on nucleotide sequences of E2 ORF partial CDS and E5 ORF complete CDS was conducted on 7 specimens, whose sequences were published in GenBank and recognized as: 2PL (Accession Number - Acc. No. KC684939) - “variant” BPV-1, 7aPL (Acc. No. KC684940) - “European” BPV-1, 10PL (Acc. No. KC693480) - “variant” BPV-1, 16PL (Acc. No. KC693484) - “variant” BPV-2, 17PL (Acc. No. KC693481) - “variant” BPV-1, 20aPL (Acc. No. KC693482) - “European” BPV-1 and 20cPL (Acc. No. KC693483) - “wild” BPV-1. Amino acid (aa) sequences of E5 ORF complete CDS were also analyzed. The E5 variant of aa sequences found in isolate 10PL (protein identification - ID: AGM 20700) is a novel variant of E5 ORF complete CDS of BPV-1 detected in equine sarcoid in Poland.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 2; 239-245
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł

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