- Tytuł:
- Isolation, molecular cloning and characterisation of two genes coding chitin deacetylase from mucor circinelloides ibt-83
- Autorzy:
-
Kaczmarek, Michał
Struszczyk-Świta, Katarzyna
Florczak, Tomasz
Szczęsna-Antczak, Mirosława
Antczak, Tadeusz - Powiązania:
- https://bibliotekanauki.pl/articles/1034731.pdf
- Data publikacji:
- 2016
- Wydawca:
- Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
- Tematy:
-
chitin deacetylase
genes
molecular cloning
sequences analysis - Opis:
- Chitosan is a linear N-deacetylated derivative of chitin, soluble in acetic solutions. The deacetylation of chitin can be achieved enzymatically using chitin deacetylase (ChDa) (EC 3.5.1.41), which hydrolyses the N-acetamido groups of N-acetyl-D-glucosamine residues in chitin and chitosan. Complementary DNA (cDNA), which encodes ChDa, was isolated from M. rouxii s well as other fungi. Chitin deacetylase activity was detected in partially purified and concentrated crude extract of the protein from Mucor circinelloides IBT-83. Additionally, two open reading frames (ORF), putatively encoding ChDa, were identified and amplified from cDNA of this strain. Each ORF was molecularly cloned and sequenced. Amino acid sequences of ChDaI and ChDaII were predicted, using nucleotide sequences of these cDNA clones, and analysed by means of bioinformatics tools.
- Źródło:
-
Progress on Chemistry and Application of Chitin and its Derivatives; 2016, 21; 93-103
1896-5644 - Pojawia się w:
- Progress on Chemistry and Application of Chitin and its Derivatives
- Dostawca treści:
- Biblioteka Nauki
Artykuł