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    Wyświetlanie 1-3 z 3
    Tytuł:
    Molecular cloning, characterization and expression of a calcium dependent protein kinase (CDPK) involved in the stress signalling in Hippeastrum x hybr.
    Autorzy:
    Szewczuk, P.
    Swiezawska, B.
    Pawelek, A.
    Jaworski, K.
    Szmidt-Jaworska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/80573.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    molecular cloning
    expression
    calcium-dependent protein kinase
    flowering plant
    Hippeastrum x hybridum
    biotic stress
    abiotic stress
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular cloning, characterization and expression of a guanylyl cyclase (HpGC1) involved in the stress signalling in Hippeastrum x hybr.
    Autorzy:
    Swiezawska, B.
    Szewczuk, P.
    Pawelek, A.
    Jaworski, K.
    Szmid-Jaworska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/81222.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    molecular cloning
    expression
    characteristics
    guanylyl cyclase
    signal transduction
    light signal transduction
    biotic stress
    abiotic stress
    stress tolerance
    Hippeastrum x hybridum
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optimization of extraction and purification of RNA from plant bulbs
    Autorzy:
    Duszyn, M.
    Swiezawska, B.
    Kwiatkowski, M.
    Jaworski, K.
    Szmidt-Jaworska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2117778.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    bulbs
    ornamental plants
    polyphenols
    polysaccharides
    RNA isolation
    Opis:
    RNA extraction involves several main stages, regardless of the method of extraction: homogenization, effective denaturation of proteins from RNA, inactivation of ribonuclease and removal of any DNA, protein, and some residual contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of polysaccharides and phenols. Several efforts have been made towards the comparison and optimization of extraction and purification methods for RNA from plant tissues. This is dictated by the necessity of obtaining RNA of a good quality and in a sufficient quantity for further molecular analyzes. Plant storage organs (such as bulbs or seeds) rich in polysaccharide and polyphenolic compounds present distinct challenges for total RNA isolation. Such components, considered in this case as contamination, may bind and co-precipitate with nucleic acids and negatively affect later assays. Since standard routine protocols yield unacceptable results in bulbs, we have designed a new method for RNA extraction. We used two modified procedures (based on CTAB and sarkosyl reagents) of RNA extraction from so called “difficult plant material” and compared them to a popular RNA isolation base on the column isolation kit and TriPure reagent. Our modified protocols dealt with problems of both RNA degradation and low yield caused by co-purification with polysaccharides present in plant bulbs. In this study we have shown that improvement of the CTAB and sarkosyl method with a lyophilization step of plant tissues leads to isolation of high quality RNA from difficult material like storage organs of bulbous plants. The main changes in the procedure compared to the previously described methods concerned the different order of lithium chloride and sodium acetate addition, lithium chloride concentration increase and modification of centrifugation conditions. Gel electrophoresis and spectrophotometer analysis confirmed the high quality and integrity of the obtained RNA. The modified procedures allowed for obtaining a satisfying amount of RNA concentration in the range from 280 to 950 ng/μl depending on the plant species. Thus, the demonstrated RNA isolation methods are efficient and can be used for plant material rich in polysaccharides, such as bulbs.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2020, 62, 2; 17-30
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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