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    Wyświetlanie 1-12 z 12
    Tytuł:
    Małopolskie Centrum Biotechnologii Uniwersytetu Jagiellońskiego. Geneza powstania, sposób funkcjonowania i znaczenie dla koncepcji uniwersytetu badawczego
    Malopolska Centre of Biotechnology of the Jagiellonian University. Origin, mode of functioning and relevance for the concept of research University
    Autorzy:
    Strzałka, Kazimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/577354.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    biotechnologia
    innowacyjność
    uniwersytet badawczy
    współpraca międzynarodowa
    zarządzanie
    biotechnology
    innovativeness
    research university
    international collaboration
    management
    Opis:
    Artykuł opisuje w przyczyny, które spowodowały powstanie Małopolskiego Centrum Biotechnologii (MCB), przedstawia historię budowy oraz dostarcza informacji o jego strukturze i sposobie działania. Podkreślono elementy stanowiące o odmienności w sposobie funkcjonowania MCB w stosunku do wydziałów uczelni. Do elementów tych należy między innymi ustanowienie Międzynarodowego Komitetu Doradczego, ocena aktywności naukowej wszystkich grup badawczych działających w MCB przez ciało zewnętrzne, nacisk na rozwój i umacnianie współpracy międzynarodowej oraz współpraca z przemysłem poprzez rozwijanie innowacyjności, tworzenie nowych technologii oraz nawiązywanie współpracy z przedsiębiorstwami. Podane zostały także przykłady osiągnięć MCB zarówno w zakresie dorobku publikacyjnego jak i skuteczności w pozyskiwaniu projektów badawczych i aplikacyjnych.
    The article describes the reasons for creation of Malopolska Centre of Biotechnology, presents the history of its construction, informs about structure and mode of its functionig. Differences in operation of MCB in comparison with the faculties have been highlighted. Among these differences are creation of an International Advisory Board, evaluation of scientifi c activity of all MCB-affi liated research groups by an external body, focus on development and strenghtening of international collaboration and cooperation with industry through development of innovativeness, creation of new technologies and collaboration with companies. Examples of MCB achievements, both in terms of published papers as well as in terms of obtained projects both for basic and applied research are provided.
    Źródło:
    Zagadnienia Naukoznawstwa; 2015, 51, 3(205); 377-385
    0044-1619
    Pojawia się w:
    Zagadnienia Naukoznawstwa
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Odwrócone struktury lipidowe i ich rola w procesach biologicznych
    The inverted lipid structures and their role in biological processes
    Autorzy:
    Latowski, Dariusz
    Strzałka, Kazimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1197889.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Przyrodników im. Kopernika
    Opis:
    Lipids may form two main types of structures: bilayer, and inverted structures. The inverted structures can form either cylindrical inverted hexagonal structures or spherical and have inverted micelles known also as cubic phase. The type of structure formed depends on the chemical texture of lipids, degree of hydratation of their polar groups’, and several other external factors, such as pH, temperature, and presence of some chemicals or salt ions. The inverted structures play an important role in several biological processes. They can promote membrane fusion. This was applied to design of lipid based delivery systems for a lot of chemicals which have to be placed inside the cells. These structures are also used in the so-called micellar enzymology and as a new approach in homogeneous enzyme immunoassay utilizing the systems of surfactant reversed micelles in organic solvents for determination of the catalytic activity of the enzymes solubilized in such systems. Cell-free translation was also observed in reversed micelles. Some enzymes as violaxanthin de-epoxidase, protein kinase C and ATP-ase require for their activity lipids creating inverted structures. These lipids are also necessary in the transport of some proteins through membranes.
    Źródło:
    Kosmos; 2007, 56, 1-2; 1-8
    0023-4249
    Pojawia się w:
    Kosmos
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Violaxanthin and diadinoxanthin de-epoxidation in various model lipid systems
    Autorzy:
    Latowski, Dariusz
    Goss, Reimund
    Bojko, Monika
    Strzałka, Kazimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1039787.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    lipids
    de-epoxidation
    diadinoxanthin cycle
    violaxanthin cycle
    Opis:
    The xanthophyll cycle is an important photoprotective process functioning in plants. One of its forms, the violaxanthin (Vx) cycle, involves interconversion between: Vx, antheraxanthin (Ax) and zeaxanthin (Zx). Another kind of the xanthophyll cycle is the diadinoxanthin (Ddx) cycle in which interconversion between Ddx and diatoxanthin (Dtx) occurs. In this study an information on molecular mechanism and regulation of these two types of the xanthophyll cycle is presented. The influence of lipids on the de-epoxidation of the xanthophyll cycle pigments was investigated, with special focus put on the significance of physical properties of the aggregates formed by inverted lipid micelles, which are necessary for activity of the xanthophyll cycle enzymes. In particular, thickness of the hydrophobic fraction of the aggregates, size of the inverted micelles, suggested by mathematical description of the structures and solubility of Vx and Ddx in various kind of lipids were studied. Obtained results show that the rate of de-epoxidation is strongly dependent on the physicochemical properties of the lipids used. The key role for enzyme activation play non-bilayer lipids and the parameters of inverted micelles such as thickness, fluidity of hydrophobic core and their diameter. The presented results show that MGDG and other non-lamellar lipids like different forms of phosphatidylethanolamine are necessary for the Vx and Ddx de-epoxidation because they provide the three-dimensional structures, which are needed for the binding of de-epoxidases and for the accessibility of Vx and Ddx to these enzymes.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 1; 101-103
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Atomic force microscopy studies of the adhesive properties of DPPC vesicles containing β-carotene
    Autorzy:
    Augustyńska, Dominika
    Jemioła-Rzemińska, Małgorzata
    Burda, Kvetoslava
    Strzałka, Kazimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1039795.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    atomic force microscopy
    dipalmitoylphosphatidylcholine
    β-carotene
    liposome adhesion
    thermal transition
    Opis:
    A role of carotenoids as modulators of physical properties of model and biological membranes has been already postulated. However, there is a lack of information on the influence of these pigments on interactions between the lipids which form such membranes. This paper applies atomic force microscopy (AFM) in to study the effects of β-carotene on the adhesion properties of DPPC multilamellar liposomes. This allowed us to gain, for the first time, a direct insight into the interactions between the components in model systems on a molecular level. We observe that the adhesive forces in DPPC multilamellar liposomes containing 1mol% of β-carotene decrease exponentially with increasing temperature, and that at about 37°C they diminish. In the case of pure liposomes the decline in adhesion is of a different nature and the adhesive forces disappear at 34°C. The adhesive forces are about 5 times higher at 31°C in the presence of β-carotene than in its absence. However, measurements using differential scanning calorimetry (DSC) showed a shift of the lamellar-to-undulled-lamellar phase transition toward lower temperatures by about 0.8±0.2°C in a system containing β-carotene. The enthalpy changes (ΔH) of this transition are similar for both systems. For the main transition, gel-to-liquid crystalline, the peak is shifted by about 0.5±0.1°C, and ΔH decreases by about 30% in liposomes treated with β-carotene in comparison to pure liposomes. Our results suggest increased cooperation between liposome components in a system with enriched β-carotene, which cause a change in phase transition temperatures. Moreover, these interactions are very sensitive to temperature.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 1; 125-128
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    EPR study of thylakoid membrane dynamics in mutants of the carotenoid biosynthesis pathway of Synechocystis sp. PCC6803
    Autorzy:
    Kłodawska, Kinga
    Malec, Przemysław
    Kis, Mihály
    Gombos, Zoltán
    Strzałka, Kazimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1039783.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    cyanobacteria
    carotenoid biosynthesis enzymes
    spin labels
    lipid desaturases
    Opis:
    EPR spectroscopy using 5-doxylstearic acid (5-SASL) and 16-doxylstearic acid (16-SASL) spin probes was used to study the fluidity of thylakoid membranes. These were isolated from wild type Synechocystis and from several mutants in genes encoding selected enzymes of the carotenoid biosynthesis pathway and/or acyl-lipid desaturases. Cyanobacteria were cultivated at 25°C and 35°C under different light regimes: photoautotrophically (PAG) and/or in light-activated heterotrophic conditions (LAHG). The relative fluidity of membranes was estimated from EPR spectra based on the empirical outermost splitting parameter in a temperature range from 15°C to 40°C. Our findings demonstrate that in native thylakoid membranes the elimination of xanthophylls decreased fluidity in the inner membrane region under optimal growth conditions (25°C) and increased it under sublethal heat stress (35°C). This indicated that the overall fluidity of native photosynthetic membranes in cyanobacteria may be influenced by the ratio of polar to non-polar carotenoid pools under different environmental conditions.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 1; 87-90
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Variations in xanthophyll composition in etiolated seedlings of Arabidopsis thaliana correlate with protochlorophyllide accumulation
    Autorzy:
    Myśliwa-Kurdziel, Beata
    Jemioła-Rzemińska, Małgorzata
    Turek, Elżbieta
    Strzałka, Kazimierz
    Malec, Przemysław
    Powiązania:
    https://bibliotekanauki.pl/articles/1039773.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    de-etiolation
    carotenoids
    HPLC
    protochlorophyllide
    Opis:
    Protochlorophyllide (Pchlide) accumulation and xantophyll composition were studied in 5-day old etiolated seedlings of three ecotypes of Arabidopsis thaliana: Columbia (Col-0), Landsberg erecta (Ler) and Wassiliewska (Ws). The total Pchlide level as measured by fluorescence spectroscopy varied significantly between ecotypes. A rapid HPLC method revealed quantitative differences in carotenoid composition. It was found that in the Ler ecotype any enhanced accumulation of Pchlide correlates with an increased level of lutein, suggesting the role of enzymes involved in lutein synthesis in cross-regulation between chlorophyll and carotenoid biosynthetic pathways. The function of the dark-accumulated carotenoid pool in seedling de-etiolation is discussed.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 1; 57-60
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression of three diadinoxanthin de-epoxidase genes of Phaeodacylum tricornutum in Escherichia coli Origami b and BL21 strain
    Autorzy:
    Bojko, Monika
    Olchawa-Pajor, Monika
    Tuleja, Urszula
    Kuczyńska, Paulina
    Strzałka, Wojciech
    Latowski, Dariusz
    Strzałka, Kazimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1039507.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    diadinoxanthin cycle
    marine diatoms
    violaxanthin
    de-epoxidation
    Opis:
    In the diadinoxanthin cycle the epoxy group is removed from diadinoxanthin and diatoxanthin is created. This conversion takes place e.g. in diatoms with the involvement of the enzyme diadinoxanthin de-epoxidase. In one of the diatom species, Phaeodactylum tricornutum (CCAP 1055/1 strain with genome sequenced) three de-epoxidase genes (PtVDE, PtVDL1, PtVDL2) have been identified, but only one of them (PtVDE) corresponds to violaxanthin de-epoxidase, an enzyme which is commonly found in higher plants. In these studies, the expression of two de-epoxidase genes of another Phaeodactylum tricornutum strain (UTEX 646), which is commonly used in diatom studies, were obtained in Origami b and BL21 E. coli strains. The molecular masses of the mature proteins are about 49 kDa and 60 kDa, respectively, for VDE and VDL2. Both enzymes are active with violaxanthin as a substrate.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 4; 857-860
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zeaxanthin epoxidation - an in vitro approach
    Autorzy:
    Kuczyńska, Paulina
    Latowski, Dariusz
    Niczyporuk, Sylvia
    Olchawa-Pajor, Monika
    Jahns, Peter
    Gruszecki, Wiesław
    Strzałka, Kazimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1039789.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Arabidopsis thaliana mutants npq1
    model system
    npq2
    xanthophyll cycle
    zeaxanthin epoxidation
    Opis:
    Zeaxanthin epoxidase (ZE) is an enzyme operating in the violaxanthin cycle, which is involved in photoprotective mechanisms. In this work model systems to study zeaxanthin (Zx) epoxidation were developed. Two assay systems are presented in which epoxidation of Zx was observed. In these assays two mutants of Arabidopsis thaliana which have active only one of the two xanthophyll cycle enzymes were used. The npq1 mutant possesses an active ZE and is thus able to convert Zx to violaxanthin (Vx) but the violaxanthin de-epoxidase (VDE) is inactive, so that Vx cannot be converted to Zx. The other mutant, npq2, possesses an active VDE and can convert exogenous Vx to Zx under strong light conditions but reverse reaction is not possible. The first assay containing thylakoids from npq1 and npq2 mutants of A. thaliana gave positive results and high efficiency of epoxidation reaction was observed. The amount of Zx was reduced by 25%. To optimize high efficiency of epoxidation reaction additional factors facilitating both fusion of the two types of thylakoids and incorporation of Zx to their membranes were also studied. The second kind of assay contained npq1 mutant thylakoids of A. thaliana supplemented with exogenous Zx and monogalactosyldiacylglycerol (MGDG). Experiments with different proportions of Zx and MGDG showed that their optimal ratio is 1:60. In such system, due to epoxidation, the amount of Zx was reduced by 38% of its initial level. The in vitro systems of Zx epoxidation described in this paper enable analysis some properties of the ZE without necessity of its isolation.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 1; 105-107
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Temperature effect on growth, and selected parameters of Phaeodactylum tricornutum in batch cultures
    Autorzy:
    Bojko, Monika
    Brzostowska, Klaudia
    Kuczyńska, Paulina
    Latowski, Dariusz
    Olchawa-Pajor, Monika
    Krzeszowiec, Weronika
    Waloszek, Andrzej
    Strzałka, Kazimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1039508.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Phaeodactylum tricornutum
    diatoms
    chlorophylls
    cell size
    photosynthesis
    Fv/Fm
    Opis:
    The effect of optimal and stress temperatures on the growth kinetics of the Phaeodactylum tricornutum CCAP/1055/1 strain (a model diatom with a known genome sequence) in batch cultures was examined. The analysis of the obtained results showed two phases of culture growth. There were significant positive correlations between OD increase of chlorophyll a chlorophyll c and protein concentration at different temperatures. The Fv/Fm parameter achieved a maximum level on the 6th or 7th day and then decreased to the values registered on the first day of observation. Genetic material undergoes gradual degradation 10 days after inoculation. The size of the cells was invariable.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 4; 861-864
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Laparosocopic appendectomy, cost-effectiveness of three different techniques used to close the appendix stump
    Autorzy:
    Matyja, Maciej
    Strzałka, Marcin
    Rembiasz, Kazimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1394238.pdf
    Data publikacji:
    2015
    Wydawca:
    Index Copernicus International
    Tematy:
    laparoscopic appendectomy
    cost-effectiveness
    results
    Opis:
    It is generally agreed that laparoscopic appendectomy is a valuable operative method. The aim of the study was to evaluate cost-effectiveness of three different techniques used to close the appendix stump. Material and methods. We conducted a retrospective study that compared three groups of patients who were operated on laparoscopically for acute appendicitis in 2013 at our institution. We used an endoscopic clip to close the appendix stump in the first group (n=20), endoscopic stapler was applied in the second group (n=20), and in the third group of patients the appendix base was closed with a laparoscopic suture (n=20). These groups were matched by age, sex and BMI. Results. The average operative cost was the highest in the second group. Cost of the laparoscopic appendectomy with the application of the endoscopic clip was significantly lower (first group) and comparable to the third group. Observed differences in total hospitalization costs were associated only with the chosen appendix stump closure technique. Conclusions. Clip closure of the appendix base is an easy and cost-effective procedure. The laparoscopic suture technique is the cheapest but technically demanding. According to our experience endoscopic stapler may be useful in some cases, although it is the most expensive method.
    Źródło:
    Polish Journal of Surgery; 2015, 87, 12; 634-637
    0032-373X
    2299-2847
    Pojawia się w:
    Polish Journal of Surgery
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Results of Laparoscopic Appendectomies Performed with the Use of Titanium Clips for Closure of the Appendicular Stump
    Autorzy:
    Strzałka, Marcin
    Matyja, Maciej
    Rembiasz, Kazimierz
    Powiązania:
    https://bibliotekanauki.pl/articles/1395843.pdf
    Data publikacji:
    2014-12-18
    Wydawca:
    Index Copernicus International
    Tematy:
    laparoscopic appendectomy
    titanium clips
    results
    Opis:
    Nowadays laparoscopy is used frequently not only in elective surgery but also in abdominal emergencies, including acute appendicitis. There are several techniques used to close the appendicular stump during laparoscopic appendectomy. The aim of the study was to present the results of minimally invasive appendectomies performed with the use of titanium clips. material and methods. Patients operated on laparoscopically for acute appendicitis with the application of titanium clips between October 2012 and December 2013 were included in the study. We reviewed retrospectively patients` data including: age, sex, duration of the surgical procedure and hospital stay, mortality, intraoperative and postoperative complication rates. Results. There were 93 patients (mean age=33.8 years, SD=15.23) in the analyzed group, including 60 men (mean age=33.5 years, SD= 15.07) and 33 women (mean age=33.9 years SD=15.26). The aver-age duration of the surgical procedure was 66 min (SD= 33.15). The average length of hospital stay was 3.38 days (SD=1.62). No intraoperative complications were observed in the analyzed group. Post-operative complication rate was low (6 cases, 6.5%). No mortality was observed. Conclusions. Laparoscopic appendectomy with the application of titanium clips for closure of the appendicular stump is safe, associated with low complication rates and should be considered as a routine technique in everyday surgical practice.
    Źródło:
    Polish Journal of Surgery; 2014, 86, 9; 418-421
    0032-373X
    2299-2847
    Pojawia się w:
    Polish Journal of Surgery
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Single-Incision Laparoscopic Cholecystectomy - can we Afford that? Cost Comparison of Different Surgical Techniques
    Autorzy:
    Matyja, Maciej
    Strzałka, Marcin
    Zychowicz, Anna
    Kołodziej, Damian
    Rembiasz, Kazimierz
    Budzyń, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1395751.pdf
    Data publikacji:
    2014-04-01
    Wydawca:
    Index Copernicus International
    Tematy:
    laparoscopy
    single access technique
    SILC
    cost effectiveness
    Opis:
    One of the most commonly performed surgeries in general surgery wards with laparoscopic technique as a method of choice is gall-bladder excision. In addition to -the commonly used conventional laparoscopic cholecystectomy single incision laparoscopic cholecystectomy is getting more and more attention. Despite many works and studies comparing these methods, there is still a shortage of results assessing efficiency of this new surgical technique. The aim of the study was to evaluate cost-effectiveness of this method in Polish financial reality. We have analyzed costs of three different surgical techniques: conventional (multi- incision) laparoscopic cholecystectomy, SILC and ‘no -port’ SILC. Material and methods. We conducted a retrospective study that compared three groups of patients who underwent treatment with conventional laparoscopic cholecystectomy (n=20), SILC (n=20) and no-port SILC (n=20). These groups were matched by age, sex and BMI. Following parameters were analyzed: complication rate, operative time, operative costs, length of hospital stay, hospitalization costs. The SILC cases were performed with one of the three-trocar SILC ports available on the market. The ‘no- port’ SILC cases were performed by single skin incision in the umbilicus, insertion of one 10 mm trocar for the operating instrument, another instrument and scope were inserted directly thorough small incisions in the aponeurosis without a dedicated port Results. The average operative cost was significantly higher in the SILC group comparing to the conventional laparoscopy group and the no-port SILC group. There was no significant difference in complication rate, operative time, length of hospital stay, or hospitalization costs between the three groups Conclusions. Currently the cost of the dedicated SILC port does not allow a regular use of this procedure in Polish financial reality. According to our experience improved cosmesis is the only advantage of the single incision laparoscopy, therefore we believe that it is reasonable to consider this technique in a a very selected group of patients.
    Źródło:
    Polish Journal of Surgery; 2014, 86, 4; 177-180
    0032-373X
    2299-2847
    Pojawia się w:
    Polish Journal of Surgery
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-12 z 12

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