Autor: Stępniewska, Zofia - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Community-level physiological profiles of microorganisms inhabiting soil contaminated with heavy metals
Autorzy:: Kuźniar, Agnieszka
Banach, Artur
Stępniewska, Zofia
Frąc, Magdalena
Oszust, Karolina
Gryta, Agata
Kłos, Marta
Wolińska, Agnieszka
Powiązania:: https://bibliotekanauki.pl/articles/973240.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Instytut Agrofizyki PAN
Opis:: The aim of the study was to assess the diffe-rences in the bacterial community physiological profiles in soils contaminated with heavy metals versus soils without metal contaminations. The study’s contaminated soil originated from the surrounding area of the Szopienice non-ferrous metal smelter (Silesia Region, Poland). The control was soil unexposed to heavy metals. Metal concentration was appraised by flame atomic absorption spectrometry, whereas the the community-level physiological profi le was determined with the Biolog EcoPlatesTM system. The soil microbiological activity in both sites was also assessed via dehydrogenase activity. The mean concentrations of metals (Cd and Zn) in contaminated soil samples were in a range from 147.27 to 12265.42 mg kg-1, and the heavy metal contamination brought about a situation where dehydrogenase activity inhibition was observed mostly in the soil surface layers. Our results demonstrated that there is diversity in the physiological profi les of microorganisms inhabiting contaminated and colntrol soils; therefore, for assessment purposes, these were treated as two clusters. Cluster I included colntrol soil samples in which microbial communities utilised most of the available substrates. Cluster II incorporated contaminated soil samples in which a smaller number of the tested substrates was utilised by the contained microorganisms. The physiological profi les of microorganisms inhabiting the contaminated and the colntrol soils are distinctly different.
Źródło:: International Agrophysics; 2018, 32, 1; 101-109
0236-8722
Pojawia się w:: International Agrophysics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Easily degradable carbon – an indicator of microbial hotspots and soil degradation
Autorzy:: Wolińska, Agnieszka
Banach, Artur
Szafranek-Nakonieczna, Anna
Stępniewska, Zofia
Błaszczyk, Mieczysław
Powiązania:: https://bibliotekanauki.pl/articles/972589.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:: agriculture
carbon
degradation
microbial
activity
soil
Opis:: The effect of arable soil was quantified against non-cultivated soil on easily degradable carbon and other selected microbiological factors, i.e. soil microbial biomass, respiration activity, and dehydrogenase activity. The intent was to ascertain whether easily degradable carbo can be useful as a sensitive indicator of both soil biological degradation and microbial hotspots indication. As a result, it was found that soil respiration activity was significantly higher (p <0.0001) in all controls, ranging between 30-60 vs. 11.5-23.7 μmol CO2 kg d.m.-1 h-1 for the arable soils. Dehydrogenase activity was significantly lower in the arable soil (down to 35-40% of the control values, p <0.001) varying depending on the soil type. The microbial biomass was also significantly higher at the non-cultivated soil (512-2807 vs. 416-1429 μg g-1 d.m., p <0.001), while easily degradable carbon ranged between 620-1209 mg kg-1 non-cultivated soil and 497-877 mg kg-1 arable soil (p <0.0001). It was demonstrated that agricultural practices affected soil properties by significantly reducing the levels of the studied parameters in relation to the control soils. The significant correlations of easily degradable carbon-respiration activity (ρ = 0.77*), easily degradable carbon-dehydrogenase activity (ρ = 0.42*), and easily degradable carbon-microbial biomass (ρ = 0.53*) reveal that easily degradable carbon is a novel, suitable factor indicative of soil biological degradation. It, therefore, could be used for evaluating the degree of soil degradation and for choosing a proper management procedure.
Źródło:: International Agrophysics; 2018, 32, 1
0236-8722
Pojawia się w:: International Agrophysics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Ektoina - przeciwstresowa cząsteczka przyszłości
Ectoine - anti-stress molecule of the future
Autorzy:: Stępniewska, Zofia
Kuźniar, Agnieszka
Pytlak, Anna
Ciepielski, Jakub
Powiązania:: https://bibliotekanauki.pl/articles/1192586.pdf
Data publikacji:: 2014
Wydawca:: Polskie Towarzystwo Przyrodników im. Kopernika
Opis:: Mikroorganizmy występują zarówno w środowiskach glebowych, jak i w ekstremalnych, m.in. w słonych jeziorach oraz kopalniach węgla czy soli. Przetrwanie w tak niekorzystnych warunkach umożliwia im obecność jednego z osmoprotektantów - ektoiny. Związek ten zapobiega utracie wody z komórek, reguluje ich turgor, nie zakłócając metabolizmu. Ektoina syntetyzowana jest przez mikroorganizmy w celu ochrony przed różnego rodzaju stresem środowiskowym, np. promieniowaniem UV lub wysoką temperaturą. Ektoina jest rzadkim aminokwasem, kwasem 1,4,5,6-tetrahydro-2-metylo-4-pyrimidyno karboksylowym o charakterze amfoterycznym. W dzisiejszych czasach znalazła szerokie zastosowanie w różnych gałęziach przemysłu. Rosnące zapotrzebowanie handlowe na ektoinę doprowadziło do szeregu działań zwiększających wydajność naturalnej biosyntezy w porównaniu do technologii chemicznych. Wśród wielu zastosowań ektoiny bardzo istotne jest wykorzystanie w medycynie. gdzie stosowana jest w celu ochrony zdrowych komórek podczas chemio- i radioterapii. Duże znaczenie posiada również w kosmetyce, gdzie dodawana jest do kremów nawilżających i produktów chroniących skórę przed promieniowaniem UV i efektem przedwczesnego foto-starzenia skóry. W biologii molekularnej wykorzystywana jest do termostabilizacji enzymów i kwasów nukleinowych, kompleksów białek-DNA i całych komórek.
Microorganisms occur in soil environments as well as in extreme environments, such as saline lakes, coal and salt mines. Their survival under unfavorable conditions is possible due to the presence of an osmoprotectant - ectoine. This compound prevents the cells from water loss and regulates their turgor without disturbing their metabolism. Ectoine is synthesized by microorganisms, in order to protect them against various types of environmental stresses such as UV radiation or heat stress. Ectoine is a rare aminoacid, 1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid of amphoteric character. Nowadays ectoine has found wide applications in different branches of the industry. The increasing demand for ectoine led to a number of actions enhancing an efficiency of its biosynthesis compared to chemical technologies. Ectoine is commonly used in medicine to protect healthy cells during chemotherapy and radiotherapy. It is also a very important compound in cosmetics as the component of moisturizers and products used for skin protection against UV radiation and prevention from its early photoaging. In molecular biology it is used for thermal stabilization of enzymes and nucleic acids, protein-DNA complexes and whole cells.
Źródło:: Kosmos; 2014, 63, 1; 25-35
0023-4249
Pojawia się w:: Kosmos
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Microbial biodiversity in arable soils is affected by agricultural practices
Autorzy:: Wolińska, Agnieszka
Górniak, Dorota
Zielenkiewicz, Urszula
Goryluk-Salmonowicz, Agata
Kuźniar, Agnieszka
Stępniewska, Zofia
Błaszczyk, Mieczysław
Powiązania:: https://bibliotekanauki.pl/articles/972735.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:: dgge
16s rrna gene
simpson diversity
bacterial communities
arable soils
Opis:: The aim of the study was to examine the differences in microbial community structure as a result of agricultural practices. Sixteen samples of cultivated and the same number of non-cultivated soils were selected. Gel bands were identified using the GelCompar software to create the presence-absence matrix, where each band represented a bacterial operational taxonomic unit. The data were used for principal-component analysis and additionally, the Shannon-Weaver index of general diversity, Simpson index of dominance and Simpson index of diversity were calculated. Denaturing gradient gel electrophoresis profiles clearly indicated differentiation of tested samples into two clusters: cultivated and non-cultivated soils. Greater numbers of dominant operational taxonomic units (65) in non-cultivated soils were noted compared to cultivated soils (47 operational taxonomic units). This implies that there was a reduction of dominant bacterial operational taxonomic units by nearly 30% in cultivated soils. Simpson dominance index expressing the number of species weighted by their abundance amounted to 1.22 in cultivated soils, whereas a 3-fold higher value (3.38) was observed in non-cultivated soils. Land-use practices seemed to be a important factors affected on biodiversity, because more than soil type determined the clustering into groups.
Źródło:: International Agrophysics; 2017, 31, 2; 259-271
0236-8722
Pojawia się w:: International Agrophysics
Dostawca treści:: Biblioteka Nauki

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