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Wyświetlanie 1-14 z 14
Tytuł:
Wystepowanie gatunkow i genotypow Giardia u ludzi i zwierzat w Wielkopolsce
Occurrence of Giardia species and genotypes in humans and animals in Wielkopolska region, Poland
Autorzy:
Solarczyk, P.
Powiązania:
https://bibliotekanauki.pl/articles/837534.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Wielkopolska
wystepowanie
pasozyty jelitowe
pasozyty zwierzat
genotyp
Giardia
pierwotniaki
gatunki
pasozyty czlowieka
Źródło:
Annals of Parasitology; 2009, 55, 4; 459-462
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Występowanie gatunków i genotypów Giardia u ludzi i zwierząt w Wielkopolsce
Occurrence of Giardia species and genotypes in humans and animals in Wielkopolska region, Poland
Autorzy:
Solarczyk, P.
Powiązania:
https://bibliotekanauki.pl/articles/2143568.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Wielkopolska
wystepowanie
pasozyty jelitowe
pasozyty zwierzat
genotyp
Giardia
pierwotniaki
gatunki
pasozyty czlowieka
Opis:
Giardia is the most common intestinal protozoan parasite found in humans and animals worldwide. Although it has been known for three hundred years, the nomenclature, taxonomy, host specificity, and pathogenicity of Giardia still arouse numerous controversies and ambiguities. Giardia is classified into six species, that are characterised by various ranges of hosts. The most dubious species is G. intestinalis, which includes a dozen or so genotypes, and only two of them (genotype A and B) have wide ranges of hosts, including humans. Moreover, in some genotype assemblages of G. intestinalis certain subgenotypes were distinguished and it was proven that in the same host species various subgenotypes of this parasite may occur. Bearing in mind the significant genetic heterogeneity of G. intestinalis and the fact that various genotypes and subgenotypes of this parasite are characterised by the broad or narrow host specificity, the data concerning the frequency of giardiosis occurrence are insufficient. It is necessary to use molecular biology techniques in order to define the genotype and/or the subgenotype of G. intestinalis that are found in humans and in certain animal species. Furthermore, since more and more pieces of evidence connected with a possibility of the sexual recombination of Giardia are gathered, it is unknown if genotypes and subgenotypes of this parasite are stable in time. The aim of this thesis was to define the frequency of Giardia occurrence in humans and animals in Wielkopolska region, to identify species and genotypes of Giardia that occur in humans and animals, as well as to obtain an axenic culture of the chosen isolates of Giardia from animals and to compare the sequence of the b-giardin gene fragment obtained from the DNA isolated from cysts and trophozoites in order to check if the axenisation of G. intestinalis leads to the selection of genotypes or if Giardia genotypes are stable in time. Altogether, 2183 faecal samples were examined for the presence of Giardia cysts; 447 faecal samples were taken from 232 persons coming from 5 cities situated in Wielkopolska, and 1736 faecal samples were obtained from 123 animal species, including: 266 faecal samples from 113 species of animals kept in the Zoological Garden in Poznań, 1286 samples from 4 species of breeding animals, 118 samples from dogs, and 66 samples from 5 species of wild animals. Faecal samples were taken from animals coming from 25 places in Wielkopolska. Moreover, seven isolates of G. intestinalis were used in the studies, which were obtained from various species of hosts and kept in an axenic in vitro culture. Microscopic, molecular and bio-informative methods were used in the studies. From each faecal sample fresh smears were made in a 0.6% solution of physiological salt and in Lugol’s solution, as well as a permanent smear stained with trichrome was made. Moreover, the following molecular techniques were implemented in the studies: DNA extraction and purification, the PCR technique (two molecular markers), electrophoresis and visualisation of PCR products, and sequencing. A fragment of the b-giardin gene was used as a molecular marker in order to define the genotype and subgenotype of Giardia. Only in the case of genotyping of two Giardia isolates obtained from Peromyscus eremicus another molecular marker (SSU rRNA)was additionally used. Some widely available computer programmes (Chromas, CAP 3, BioEdit, BLASTn, MEGA version 4.0) were utilised in the analysis of the sequence of the b-giardin gene fragment and in the phylogenetic analysis. The culture of Giardia trophozoites was established to compare the sequence of the partial b-giardin gene from cysts and trophozoites. Concentration and purification of Giardia cysts in the saccharose gradient, and the excystation technique were applied in the studies to obtaining an axenic in vitro culture. In this study, Giardia cysts were found in 12 faecal samples obtained from 3 persons and 5 animal species. Giardia cysts were found only in faecal samples from humans living in Poznań and the samples obtained from animals coming from Poznań and around Puszczykowo. The highest frequency of infection was stated in domestic animals (2.5%) and in animals kept in the Zoological Garden (2.0%), whereas a slightly lower frequency was noticed in wild animals (1.5%) and in humans (1.3%). No Giardia cysts were found in the faecal samples collected from breeding animals. Two new species of Giardia hosts were identified, namely Rhinella marina and Peromyscus eremicus; however, due to a minimal amount of faecal samples supplied for the study it was impossible to define the species and genotype of this parasite. PCR products (the partial of b-giardin gene) were obtained in seven faecal samples out of the ten studied, including three samples from people and four faecal samples derived from three animal species (i.e. dog, tamandua, red deer). Moreover, molecular characterization of seven Giardia isolates from three persons and four animal species (red-bellied monkey, silver marmoset, Thomson’s gazelle, and sheep) kept in an axenic in vitro culture was performed. Based on the b-giardin sequence fragment analysis, four assemblages of G. intestinalis genotypes were identified (A, B, C and D). In humans, A and B G. intestinalis genotypes and three subgenotypes, including a cosmopolitan subgenotype A2 and two new subgenotypes A and B were detected. Furthermore, four G. intestinalis genotypes were found in animals, including three genotypes which are non-infectious to humans, namely: genotypes C and D in dogs and a cervids-specific genotype A in red deer (Cervus elaphus), which indicate that these animals do not constitute the source of infection to humans. On the other hand, in a tamandua from the Zoological Garden in Poznań a new subgenotype B of G. intestinalis was identified, which due to a close relationship with Giardia isolates obtained from humans is potentially infectious to man. In none of the studied faecal samples a mixed infection of Giardia was found. To date, nine sequences of the partial b-giardin gene have been deposited in the National Center for Biotechnology Information (NCBI), including two sequences of Giardia isolates obtained from humans (GenBank accession numbers FJ009207, FJ009208), three sequences of isolate obtained from red deer (GenBank accession numbers EU621373, EU626198, EU216429), two sequences of both Giardia isolates obtained from dogs (GenBank accession numbers FJ009205, FJ009206), and the single sequences obtained from tamandua (GenBank accession number FJ009209) and from Thomson’s gazelle (GenBank accession number EU626199). According to the literature, an axenic in vitro culture of G. intestinalis was obtained from a red deer for the first time. Based on the analysis of the sequence of the b-giardin gene fragment obtained from the DNA isolated from cysts and trophozoites it was proven that the red deer was infected with a single population of Giardia and that during the axenisation of the culture no mutation in the DNA of the parasite’s trophozoites took place. Probably the time distance that the DNA was isolated from the trophozoites kept in the culture was too short to cause the mutation. This suggestion is confirmed by the results of the genotyping of seven G. intestinalis isolates obtained from various host species and kept in an axenic in vitro culture for at least a number of years. Based on the molecular characteristics it was stated that all the studied isolates from the axenic culture were identical and belonged to the same assemblage B. The comparision with the sequences from GenBank database revealed that all mentioned isolates were 99% similar to the sequence of Giardia Nij5 isolate obtained from a person from the Netherlands and characterised as genotype B1. Due to the sameness of the molecular marker sequences it seems improbable that the identical G. intestinalis genotype occurred in various time periods (the largest difference was 14 years) in humans and in a number of animal species in diverse areas of Wielkopolska region. Quite opposite, the long-term keeping of these isolates in the homogenous conditions of an axenic in vitro culture leads to the selection of a genotype or proves the instability of genotypes of this parasite. Long-term studies need to be conducted in order to verify these hypothesis. Their results will have a key meaning in explaining the genetic structure of the Giardia population and in understanding the molecular epidemiology of giardiosis.
Źródło:
Wiadomości Parazytologiczne; 2009, 55, 4; 459-462
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence and multilocus genotyping of Giardia from animals at the zoo of Poznan, Poland
Autorzy:
Solarczyk, P.
Majewska, A.
Powiązania:
https://bibliotekanauki.pl/articles/2143383.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
prevalence
genotyping
Giardia
animal
Polska
zoo animal
Poznan Zoological Garden
zoonotic transmission
Giardia duodenalis
cyst
feces sample
genotype
identification
Opis:
In this study total of 266 fecal samples from 242 animals belonging to 113 species kept in the Poznan Zoological Garden were examined for Giardia. The cysts of Giardia were found only in five samples of feces collected from a giant toad (Bufo marinus), tamandua (Tamandua tetradactyla) and three individuals of cactus mouse (Peromyscus eremicus). Fragments of β-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes were successfully amplified only from the Giardia isolate obtained from the tamandua. Sequence and phylogenetic analyses showed that the Giardia isolate from the tamandua belonged to the B assemblage and showed homologies of 99% to 100% at bg, gdh and tpi loci of the same markers of parasites isolated from humans and animals in various parts of the world. This is the first molecular characterization of G. duodenalis from tamandua.
Źródło:
Wiadomości Parazytologiczne; 2011, 57, 3; 169-173
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence and multilocus genotyping of Giardia from animals at the zoo of Poznan, Poland
Autorzy:
Solarczyk, P.
Majewska, A.
Powiązania:
https://bibliotekanauki.pl/articles/840502.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
prevalence
genotyping
Giardia
animal
Polska
zoo animal
Poznan Zoological Garden
zoonotic transmission
Giardia duodenalis
cyst
feces sample
genotype
identification
Źródło:
Annals of Parasitology; 2011, 57, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Trichinella spiralis in road-killed raccoon dogs (Nyctereutes procyonoides) in western Poland
Autorzy:
Osten-Sacken, N.
Solarczyk, P.
Powiązania:
https://bibliotekanauki.pl/articles/6724.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Opis:
Trichinellosis is still one of the most important food-borne parasitic zoonoses and is considered as a threat to public health worldwide. The aim of this study was to use genotyping techniques to determine the prevalence of Trichinella species in wild raccoon dogs (Nyctereutes procyonoides) in western Poland. The infection rate in raccoon dogs was 0.8%. All infections were due to T. spiralis.
Źródło:
Annals of Parasitology; 2016, 62, 1
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genotypowanie izolatow Giardia duodenalis uzyskanych od ludzi w zachodnio-centralnej Polsce
Genotype analysis of Giardia duodenalis isolated obtained from humans in West-central Poland
Autorzy:
Solarczyk, P
Werner, A.
Majewska, A.C.
Powiązania:
https://bibliotekanauki.pl/articles/838606.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Giardia duodenalis
genotypowanie
genotyp
pasozyty jelitowe
pasozyty czlowieka
Polska Srodkowo-Zachodnia
Źródło:
Annals of Parasitology; 2010, 56, 2; 171-177
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genotypowanie izolatów Giardia duodenalis uzyskanych od ludzi w zachodnio-centralnej Polsce
Genotype analysis of Giardia duodenalis isolated obtained from humans in West-central Poland
Autorzy:
Solarczyk, P.
Werner, A.
Majewska, A.C.
Powiązania:
https://bibliotekanauki.pl/articles/2143436.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Giardia duodenalis
genotypowanie
genotyp
pasozyty jelitowe
pasozyty czlowieka
Polska Srodkowo-Zachodnia
Opis:
Giardia duodenalis (syn. G. intestinalis, G. lamblia) is a cosmopolitan flagellate organism belonging to the most common intestinal protozoan parasites of humans and animals. Great genetic heterogeneity has been found within G. duodenalis, where only genotypes representing assemblages A and B have zoonotic potential. Fecal samples (447 specimens) obtained from 232 humans in West-central region of Poland were examined by microscopy and PCR. The total prevalence of Giardia in humans was 1.3%. DNA was extracted from three positive fecal samples and PCR products were obtained after amplification using the β-giardin primers G7 and G759. Sequence and phylogenetic analyses showed that G. duodenalis isolates from humans belonged to A and B genotypes. Moreover, three subgenotypes, including a cosmopolitan subgenotype A2 and two new subgenotypes A and B were detected.
Źródło:
Wiadomości Parazytologiczne; 2010, 56, 2; 171-177
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Trends in animal production from organic farming [review]
Autorzy:
Wójcik, W.
Solarczyk, P.
Łukasiewicz, M.
Puppel, K.
Kuczyńska, B.
Powiązania:
https://bibliotekanauki.pl/articles/105818.pdf
Data publikacji:
2018
Wydawca:
Centrum Badań i Innowacji Pro-Akademia
Tematy:
ecological production
organic
Polska
agriculture
farms
produkcja ekologiczna
Polska
rolnictwo
gospodarstwa
Opis:
Organic farming is an alternative method for dynamic agricultural system. Products that are obtained from organic farming are referred to as ecological or organic food [1]. These include products of animal origin that come from organic farming [2]. In the case of animal production in organic farming there are particular requirements for breed, animal welfare and feeding. Additionally, the origins of animals is also of crucial importance since, basically, such animals should be purchased from organic farms. However, there can be exceptions to this requirement, for instance, if the number of animals of a particular species or of a specific breed is not sufficient [3]. The main idea behind organic production is obtaining plant or animal products maintaining good soil structure, clean water and adjusting to the natural rhythm of nature. Enhancement of the social status has led to the situation where consumers pay more attention to the quality and origins of the products they choose. Numerous scientific papers from recent years, based on consumer’s opinion, show substantial impact of welfare system on the quality of animal products. Since ‘90s there has been a systematic surge of interest in products from ecological systems and demand for these, which in turn affect the development of this agricultural sector. The aim of the work is to compare the changes in organic production over the last 26 years in Poland with reference to the situation in Europe and whole globe. The research has been done on the basis of statistics since 1990 up till now as well as on scientific studies. Nowadays, there are increasing numbers of farms and redirections of production, as well as changes in the sizes of farms producing organic food.
Źródło:
Acta Innovations; 2018, 28; 32-39
2300-5599
Pojawia się w:
Acta Innovations
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The presence of pathogenic genotypes of free-living amoebae isolated from sandboxes in children’s playgrounds in the city of Poznan, Poland
Autorzy:
Cholewinski, M.
Solarczyk, P.
Derda, M.
Wojtkowiak-Giera, A.
Hadas, E.
Powiązania:
https://bibliotekanauki.pl/articles/5871.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pathogenic amoeba
genotype
free-living amoeba
amoeba
isolation
Acanthamoeba
human disease
granulomatous amoebic encephalitis
sandbox
child
playground
Poznan city
Polska
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Giardia prevalence in wild cervids in Poland
Autorzy:
Majewska, A.C.
Solarczyk, P.
Moskwa, B.
Cabaj, W.
Jankowska, W.
Nowosad, P.
Powiązania:
https://bibliotekanauki.pl/articles/6186.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Giardia
cyst
prevalence
wild animal
cervid
Dama dama
fallow deer
red deer
Cervus elaphus
roe deer
Capreolus capreolus
moose
Alces alces
fecal sample
cervid zob.tez deer
Polska
Źródło:
Annals of Parasitology; 2012, 58, 4
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
PCR, Real-Time PCR and molecular characteristic of Toxoplasma gondii isolates from wild aquatic birds: preliminary analysis
Autorzy:
Lass, A.
Solarczyk, P.
Kavetska, K.
Dzierzba, E.
Werner, A.
Majewska, A.C.
Powiązania:
https://bibliotekanauki.pl/articles/6029.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
polymerase chain reaction
real-time PCR method
molecular characteristics
Toxoplasma gondii
isolate
parasite
wild animal
bird
aquatic bird
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The raccoon (Procyon lotor) as a reservoir of zoonotic diseases
Autorzy:
Solarczyk, P.
Osten-Sacken, N.
Heddergott, M.
Frantz, A.
Jankowska, W.
Majewska, A.C.
Powiązania:
https://bibliotekanauki.pl/articles/5791.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
racoon
Procyon lotor
Baylisascaris procyonis
Giardia
reservoir
zoonotic disease
prevalence
helminth
intestinal parasite
Protozoa
human disease
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dairy cattle crossbreeding and milk production
Krzyżowanie bydła ras mlecznych a użytkowość mleczna
Autorzy:
Slosarz, J.
Solarczyk, P.
Kunowska-Slosarz, M.
Nalecz-Tarwacka, T.
Golebiewski, M.
Wojcik, A.
Powiązania:
https://bibliotekanauki.pl/articles/2710.pdf
Data publikacji:
2016
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
dairy cattle
crossbreeding
milk production
milk composition
Opis:
Dairy cattle crossbreedingand milk production.The aim of this study was to compare the results of milk performance of purebred Polish Holstein-Frisian (PHF) cows and their crossbreeds (PHF x SRB). Data for the analysis originated from the Polish Federation of Dairy Cattle Breeder and Producers - cows reports. The results indicate a positive influence of the breed on milk composition: fat, protein and dry matter content and somatic cell count. purebred Polish Holstein-Frisian (PHF) cows had significantly higher (p≤0.05) milk yield for both the 100 and 305 days milking. The milk production of MM cows is lower by 15.89%, at 100 days of lactation, and by 34.57% in the full lactation than PHF.
Krzyżowanie bydła ras mlecznych a użytkowość mleczna.Celem pracy było porównanie wyników użytkowości mlecznej czystorasowych krów (PHF) oraz mieszańców międzyrasowych F1 (PHF x SR). Dane do analizy pochodziły z raportów wynikowych i kart jałówki – krowy. Uzyskane wyniki wskazują na pozytywny wpływ rasy SR na skład mleka tj. procentową zawartość tłuszczu, białka i suchej masy oraz liczbę komórek somatycznych. Czystorasowe krowy rasy PHF charakteryzowały się istotnie wyższą (p≤0.05)wydajnością mleka zarówno za 100 i 305 dni doju. Wydajność mleka mieszańców za 100 dni laktacji była niższa o 15.89%, i 34.57% za laktację standardową niż wydajność krów rasy PHF.
Źródło:
Annals of Warsaw University of Life Sciences - SGGW. Animal Science; 2016, 55[2]
1898-8830
Pojawia się w:
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zapis procesów sedymentacji fluwialnej i biogenicznej w osadach dna Doliny Dolnej Wisły
Record of fluvial and biogenic sedimentation processes in sediments of the Lower Vistula Valley floor
Autorzy:
Kordowski, J.
Gamrat, W.
Gierszewski, P.
Kubiak-Wójcicka, K.
Szmańda, J. B.
Tyszkowski, S.
Solarczyk, A.
Powiązania:
https://bibliotekanauki.pl/articles/294952.pdf
Data publikacji:
2013
Wydawca:
Stowarzyszenie Geomorfologów Polskich
Tematy:
równina zalewowa
zamulanie starorzeczy
Dolina Dolnej Wisły
floodplain
silting of oxbows
Lower Vistula Valley
Opis:
W artykule przedstawiono przegląd osadów powodziowych i biogenicznych zdeponowanych w dnie Doliny Dolnej Wisły od okolic Górska, w środkowej części Kotliny Toruńskiej, po okolice Grudziądza. Omówiono piaszczyste osady powodziowe, osady basenów popowodziowych i mineralne osady jeziorzysk dolinnych. Dokonano analizy podstawowych własności utworów biogenicznych i ich interpretacji genetycznej. Omówiono też wyniki badań nad rekonstrukcją układu koryta Wisły w ciągu ostatnich 200 lat w Kotlinie Grudziądzkiej i Basenie Unisławskim oraz problem powolnego zamulania starorzeczy powstałych przed regulacją i po regulacji hydrotechnicznej w końcu XIX w. Wyjaśniono kwestię gradientu zawartości węglanowej materii rozpuszczonej w dnie doliny i jego wpływ na sedymentację osadów. Zwrócono uwagę na podobieństwa i różnice w transporcie materii rozpuszczonej i zawieszonej między korytem Wisły i starorzeczami na przykładzie starorzecza Starej Wisły i koryta Wisły w Bratwinie.
The paper is a review of flood and biogenic sediments which have been deposited on the Lower Vistula Valley floor from the vicinity of Górsk to Grudziądz town. Sandy flood sediments, flood basin sediments and mineral infillings of former back swamps are described. Biogenic sediments are also discussed and genetically interpreted. The results of the Vistula channel reconstruction in Grudziądz Basin and Unisław Basin in the last 200 years are also presented. The reconstruction is strongly linked with the problem of relatively slow siltation of oxbows after the hydrotechnical regulation at the end of XIX-th century. The question of the carbonates gradient in floodplain sediments is also explained. The differences and similarities of the dissolved and suspended matter transport between the main channel and fluvial lake is shown on the example of the oxbow lake and Vistula river channel in Bratwin.
Źródło:
Landform Analysis; 2013, 25; 77-93
1429-799X
Pojawia się w:
Landform Analysis
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-14 z 14

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