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Wyświetlanie 1-15 z 44
Tytuł:
Wykrywanie Borrelia burgdorferi sensu lato w kleszczach Ixodes ricinus metoda lancuchowej reakcji polimerazy [PCR]
Autorzy:
Skotarczak, B
Powiązania:
https://bibliotekanauki.pl/articles/838375.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
czynniki chorobotworcze
kleszcze
wykrywanie drobnoustrojow
przenoszenie chorob
parazytologia
Ixodes ricinus
lancuchowa reakcja polimerazy
Borrelia burgdorferi
Opis:
Attempts were made to identify the causative orgamsm of Lyme disease in Szczecin from tick Ixodes ricinus as a vector. Ticks were collected in 1997 year in forest areas of Szczecin, from localites associated with numerous attendance of people. The method used in this study was the polymerase chain reaction (PCR) on the flagellin structural gene fla of Borrelia burgdorferi sensu stricto. The flagellin PCR primer set reaction was conservative for B. burgdorferi sensu stricto, B. afzelii and B. garinii. The overall prevalence of B. burgdorferi sensu lato, in tick population studied was 8.8%. The female, nymphs and larves of Ixodes ricinus were infected almost just the some - about 10%, when the male 2.5% only.
Źródło:
Annals of Parasitology; 2000, 46, 1; 93-99
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Babeszjoza czlowieka i psa domowego; etiologia, chorobotworczosc, diagnostyka
Babesiosis of human and domestic dog; ethiology, pathogenesis, diagnostics
Autorzy:
Skotarczak, B
Powiązania:
https://bibliotekanauki.pl/articles/836747.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka molekularna
choroby pasozytnicze
markery genetyczne
chorobotworczosc
diagnostyka
babeszjoza
choroby czlowieka
zwierzeta domowe
markery molekularne
parazytologia
pies domowy
etiologia
choroby zwierzat
Źródło:
Annals of Parasitology; 2007, 53, 4; 271-280
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of companion animals in the environmental circulation of tick-borne bacterial pathogens
Autorzy:
Skotarczak, B.
Powiązania:
https://bibliotekanauki.pl/articles/2081833.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
‘One Health’ concept
ticks
Ixodes ricinus
cats
dogs
bacterial tick-borne diseases
epidemiology
molecular tools
risk for
pet owners
Opis:
Ticks are known as vectors of a wide range of pathogens of medical and veterinary importance; some of them of zoonotic concern constitute a hazard for the emergence of tick-borne diseases shared between humans and domestic animals and becoming a part of the ‘One Health’ concept. Canine and feline tick-borne diseases have emerged in recent years, performing an extensive geographic distribution and enlarged global prevalence. The present review focuses on the recent epidemiological studies on the emergence of tick-borne bacterial pathogens in dogs and cats, and the discussion whether pet ownership increases the risk of tick-borne diseases. A lot of data provide confirmation that dogs and cats themselves may substantially contribute to the circulation of the ticks and tick-borne bacterial pathogens in the environment. Molecular diagnostics of tick-borne pathogens infections generates a lot of problems like the choice of molecular methods and molecular markers for the detection of bacterial genomic DNA, but play an important role in the diagnosis of infections. The study provides some insight into molecular diagnostic techniques and new potentially recognized bacterial pathogens of this group. Protecting human and companion animal health from vector-borne infections requires controlling vector populations, containing development of novel, practicable strategies that will limit vectors and transmission of vectorborne disease pathogens.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 3; 473-480
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adaptation factors of Borrelia for host and vectors
Autorzy:
Skotarczak, B
Powiązania:
https://bibliotekanauki.pl/articles/50116.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
Borrelia burgdorferi
adaptation factor
host
metabolism
genome
quorum sensing
protein expression
vls gene
spirochete zob.spirochaete
spirochaete
human disease
etiological factor
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 1-8
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Progress in the molecular methods for the detection and genetic characterization of Cryptosporidium in water samples
Autorzy:
Skotarczak, B
Powiązania:
https://bibliotekanauki.pl/articles/52184.pdf
Data publikacji:
2010
Wydawca:
Instytut Medycyny Wsi
Tematy:
protozoan parasite
Cryptosporidium
genetic characteristics
molecular method
detection
water sample
food-borne way
oocyst
infection control
Źródło:
Annals of Agricultural and Environmental Medicine; 2010, 17, 1; 1-8
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of ticks in transmission cycle of Toxoplasma gondii
Autorzy:
Skotarczak, B.
Powiązania:
https://bibliotekanauki.pl/articles/6137.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Opis:
Toxoplasmosis is globally distributed, water- and food borne zoonosis caused by the single protozoan Toxoplasma gondii and probably one-third of the world’s human population is infected with this pathogen. Domestic and wild felids are definitive hosts of this pathogen and intermediate hosts for great variety of other homoeothermic animals. Human as other of the intermediate hosts may become infected in the main route of infection; it is the ingestion of parasite oocysts in contaminated water or soil and undercooked meat. However, the mechanism which this parasite uses to so large spread is not fully understood, because oral transmission does not explain the common event of this parasite in a variety of hosts, such as herbivorous animals or rodents and birds, as well as routes of spread to domestic hosts. Such a wide circle of hosts suggests a possibility of other paths of transmission and a role of ticks, the bloodseeking arthropods was considered in the transmission of T. gondii.
Źródło:
Annals of Parasitology; 2016, 62, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Babeszjoza człowieka i psa domowego; etiologia, chorobotwórczość, diagnostyka
Babesiosis of human and domestic dog; ethiology, pathogenesis, diagnostics
Autorzy:
Skotarczak, B.
Powiązania:
https://bibliotekanauki.pl/articles/2144073.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka molekularna
choroby pasozytnicze
markery genetyczne
chorobotworczosc
diagnostyka
babeszjoza
choroby czlowieka
zwierzeta domowe
markery molekularne
parazytologia
pies domowy
etiologia
choroby zwierzat
Opis:
This article presents the current state of our knowledge on babesiosis (piroplasmosis), one of the dangerous, invasive disease of humans and animals, transmitted by ticks. It is included among emerging diseases because its spread and significance have increased in recent years. This sickness is caused by intraerythrocytic parasites belonging to the Babesia species and it is a well−known zoonosis occurring in animals; as a human disease it was unknown almost till the first half of the last century. The intensified migration of human population and human interference in a forest biotope caused that number of recognized cases has grown considerably in recent years. Piroplasmosis in dogs is widely spread all over the world and it is caused by several Babesia species. The principal etiological factor of babesiosis in dogs is B. canis, which turned out to be a collective species represented by three subspecies for which the vectors are three different species of ticks. Their geographical extent indicates the endemic areas for this often fatal disease. A technique, the most often applied in the detection of Babesia is a full blood smear stained with Giemsa or Wright method. However, the estimation of the specimen depends to a large extent on the experience of the diagnostician. The immunological and serological methods are characterized with a high specificity and sensitivity but there are patients in which the false negative results have been obtained. Therefore, the traditional methods have been complemented or even ousted by the molecular methods, in which polymerase chain reaction (PCR) brings the biggest profits. However, the standardization of this technique still remains under elaboration. The usefulness of the PCR protocol has been tested with different molecular destinations from which sequences of genes encoding rRNA for small ribosomal subunit are taken into consideration. Within ribosome, the evolutionally conservative areas can be distinguished, i.e. having the nucleotide sequences similar to the majority or all Babesia species and to others closely related to them. Such construction of gene enables designing of starters complementary to conservative sites to PCR, detecting a large group of related organisms. Another molecular marker allowing on the accurate identification of Babesia is gene encoding the ß-tubuline protein. There are two introns within this gene, from which the first one shows a big variability with regard to the length as well as to the nucleotide sequence, therefore, the PCR products show a diverse length depending on the Babesia species. But these differences are too small for some species and, confirming methods that extend time of diagnostics are essential. The other genes which sequences can be used as molecular aim to the detection of DNA and Babesia species diversification are genes encoding the Heat Shock Proteins HSP 70. However, the gene hsp 70 shows a big conservatism of the nucleotide sequence even between the non related organisms; therefore, this method, based on the amplification of whole genome or its fragments, applies mainly in analysis of molecular phylogenetic.
Źródło:
Wiadomości Parazytologiczne; 2007, 53, 4; 271-280
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykrywanie Borrelia burgdorferi sensu lato w kleszczach Ixodes ricinus metoda łańcuchowej reakcji polimerazy [PCR]
Autorzy:
Skotarczak, B.
Powiązania:
https://bibliotekanauki.pl/articles/2148520.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
czynniki chorobotworcze
kleszcze
wykrywanie drobnoustrojow
przenoszenie chorob
parazytologia
Ixodes ricinus
lancuchowa reakcja polimerazy
Borrelia burgdorferi
Opis:
Attempts were made to identify the causative orgamsm of Lyme disease in Szczecin from tick Ixodes ricinus as a vector. Ticks were collected in 1997 year in forest areas of Szczecin, from localites associated with numerous attendance of people. The method used in this study was the polymerase chain reaction (PCR) on the flagellin structural gene fla of Borrelia burgdorferi sensu stricto. The flagellin PCR primer set reaction was conservative for B. burgdorferi sensu stricto, B. afzelii and B. garinii. The overall prevalence of B. burgdorferi sensu lato, in tick population studied was 8.8%. The female, nymphs and larves of Ixodes ricinus were infected almost just the some - about 10%, when the male 2.5% only.
Źródło:
Wiadomości Parazytologiczne; 2000, 46, 1; 93-99
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cytofotometryczna analiza trofozoitów i cyst Balantidium coli
Autorzy:
Skotarczak, B.
Powiązania:
https://bibliotekanauki.pl/articles/2149007.pdf
Data publikacji:
1996
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
RNA
Balantidium coli
kwasy nukleinowe
cysty
trofozoity
pierwotniaki
parazytologia
pasozyty
analiza cytofotometryczna
orzeski
DNA
Źródło:
Wiadomości Parazytologiczne; 1996, 42, 2; 159-169
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wystepowanie kretkow Borrelia burgdorferi s.l. u kleszczy Ixodes ricinus w lasach wojewodztwa szczecinskiego
Autorzy:
Skotarczak, B
Wodecka, B.
Powiązania:
https://bibliotekanauki.pl/articles/841322.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
czynniki chorobotworcze
kleszcze
woj.szczecinskie
przenoszenie chorob
parazytologia
lasy
Ixodes ricinus
Borrelia burgdorferi
bakterie
kretki
Opis:
Presently acquired results are the outcome of, as for today, pilot-scale, preliminary study, although they were based on a tick (Ixodes ricinus) population of 533 individuals, collected from the vicinity of Szczecin. The areas sampled have been considered as recreational, which means they are frequented by a large number of people. The ticks were collected in 1996 in two seasons: spring-summer and summer-autumn. The overall prevalence of the spirochetes B. burgdorferi sensu lato, in the tick population studied, was 12.6%, while that of the spring-summer season reached 25.5%, decreasing in the summer-autumn season down to 2.3%.
Źródło:
Annals of Parasitology; 1998, 44, 2; 227-232
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetyczna zmiennosc Borrelia burgdorferi s.l. u kleszczy Ixodes ricinus zebranych w polnocnozachodniej Polsce
Autorzy:
Wodecka, B
Skotarczak, B.
Powiązania:
https://bibliotekanauki.pl/articles/836660.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wektory bakterii
kleszcze
zmiennosc genetyczna
parazytologia
Ixodes ricinus
Borrelia burgdorferi
Polska Polnocno-Zachodnia
Opis:
Borrelia burgdorferi sensu lato (s. I.), the etiological agent of Lyme diesease, is transmitted by the bite of Ixodes ricinus. During May and September 1999, field surveys on Lyme disease spirochetes were conducted in three locations of a region of north-west Poland, known as recreational districts visited by many people. The ticks Ixodes ricinus were collected in natural habitats by dragging a flanel cloth over the vegetation. Sex and developmental stage of each tick were determined. Based on a polymerase chain reaction test with primers that recognize a chromosomal gene of all strains, out of the total 1414 specimens collected, 126 (8.9%) were found to be infected. the species B. burgdorferi s. 1. comprises at least three pathogenic genomospecies, B. burgdorferi sensu stricto (s. s.), Borrelia garinii, and Borerelia afzelii, witch could be distinguished in nested-PCR tests with species-specific primers. B. burgdorferi s. s. was most prevalent (96% of infected ticks), followed by B. garinii (1.3%), and B. afzelii. was not found. Of the infected ticks, over the 99% were infected with a single species; one specimens was infected with two species. For 4 ticks, the infecting species could not be identied. The difference in rates of prevalence was observed among the tree locations (17%-5.3%-3.2%).
Źródło:
Annals of Parasitology; 2000, 46, 4; 475-485
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetyczna zmienność Borrelia burgdorferi s.l. u kleszczy Ixodes ricinus zebranych w polnocnozachodniej Polsce
GENETIC DIVERSITY OF BORRELIA BURGDORFERI SENSU LATO IN IXODES RICINUS TICKS COLLECTED IN NORTH-WEST POLAND
Autorzy:
Wodecka, B.
Skotarczak, B.
Powiązania:
https://bibliotekanauki.pl/articles/2148613.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wektory bakterii
kleszcze
zmiennosc genetyczna
parazytologia
Ixodes ricinus
Borrelia burgdorferi
Polska Polnocno-Zachodnia
Opis:
Borrelia burgdorferi sensu lato (s. I.), the etiological agent of Lyme diesease, is transmitted by the bite of Ixodes ricinus. During May and September 1999, field surveys on Lyme disease spirochetes were conducted in three locations of a region of north-west Poland, known as recreational districts visited by many people. The ticks Ixodes ricinus were collected in natural habitats by dragging a flanel cloth over the vegetation. Sex and developmental stage of each tick were determined. Based on a polymerase chain reaction test with primers that recognize a chromosomal gene of all strains, out of the total 1414 specimens collected, 126 (8.9%) were found to be infected. the species B. burgdorferi s. 1. comprises at least three pathogenic genomospecies, B. burgdorferi sensu stricto (s. s.), Borrelia garinii, and Borerelia afzelii, witch could be distinguished in nested-PCR tests with species-specific primers. B. burgdorferi s. s. was most prevalent (96% of infected ticks), followed by B. garinii (1.3%), and B. afzelii. was not found. Of the infected ticks, over the 99% were infected with a single species; one specimens was infected with two species. For 4 ticks, the infecting species could not be identied. The difference in rates of prevalence was observed among the tree locations (17%-5.3%-3.2%).
Źródło:
Wiadomości Parazytologiczne; 2000, 46, 4; 475-485
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Występowanie Ixodes ricinus na wybranych terenach rekreacyjnych b. województwa szczecińskiego. Cz.3
Autorzy:
Skotarczak, B.
Wodecka, B.
Powiązania:
https://bibliotekanauki.pl/articles/2147691.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wystepowanie
tereny rekreacyjne
kleszcze
woj.szczecinskie
parazytologia
woj.zachodniopomorskie
Ixodes ricinus
Opis:
The occurrence of Ixodes ricinus in the selected recreative areas in the province of Szczecin. Part III. Within the last years, the incidences of diseascs transmitted by Ixodes ricinus tick have rapidly increased. We estimated the occurrence of Ixodes ricinus in the popuJlr recreation urban areas in Szczecin and in the Province of Szczecin. The study was carried out in 1999, with two samples at each site, and were compared with data of 1998. The temperature and humidity of air were measured. The most ticks were found in the range of 70-80% relative humidity of air. Among 3.198 specimens collected 59.5% were nymphs, 19.0% larvae, 11.1 % females and 10.4% males. The nymphs were the most frequent in spring and in autumn, while the larvae were most frequent in autumn (26.2%) then in spring (11.9%).
Źródło:
Wiadomości Parazytologiczne; 2002, 48, 2; 201-206
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 44

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