- Tytuł:
- Limited GADD45α expression and function in IL-1β toxicity towards insulin-producing cells
- Autorzy:
-
Skalniak, Lukasz
Gurgul-Convey, Ewa
Okreglicka, Katarzyna
Skalniak, Anna
Jura, Jolanta - Powiązania:
- https://bibliotekanauki.pl/articles/1039450.pdf
- Data publikacji:
- 2013
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
GADD45
IL-1β
insulin-producing cells
type 1 diabetes - Opis:
- Growth arrest and DNA damage-inducible (GADD) 45 proteins are regulators of cell death and survival. The proinflammatory cytokine IL-1β strongly increases the level of the transcript encoding GADD45α in rat insulin-producing INS-1E cells. The activation of Gadd45α gene is clearly dependent on JNK and NF-κB activation and the synthesis of the secondary mediator nitric oxide (NO). Interestingly, the observed twelve-fold increase in the GADD45α-coding transcript level is not followed by increased expression of GADD45α at the protein level. An analysis of IL-1β toxicity in INS-1E cells overexpressing GADD45α revealed no correlation between the GADD45α protein level and the sensitivity to IL-1β toxicity. These findings suggest that the potential engagement of GADD45α in IL-1β toxicity towards beta cells is limited to the effects induced by the basal expression level of this protein.
- Źródło:
-
Acta Biochimica Polonica; 2013, 60, 4; 595-602
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł