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Wyszukujesz frazę "Siemionek, J." wg kryterium: Autor


Wyświetlanie 1-4 z 4
Tytuł:
Quantitative PCR High-Resolution Melting (qPCR-HRM) curve analysis, a new approach to rapid detection and differentiation of bovine papillomavirus detected in equine sarcoids
Autorzy:
Szczerba-Turek, A.
Bancerz-Kisiel, A.
Lipczynska, K.
Siemionek, J.
Ras, A.
Platt-Samoraj, A.
Szweda, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087954.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the study was to evaluate a novel diagnostic scheme which combines quantitative PCR and High-Resolution Melting (qPCR-HRM) curve analysis for rapid differentiation based on E5 partial CDS of bovine papillomavirus type 1 or 2 (BPV-1 or BPV-2), and to perform a phylogenetic analysis of the complete CDS of the E5 gene of BPV detected in equine sarcoids. Samples of 38 skin lesions obtained from 27 horses were collected for molecular examinations. All lesions were clinically diagnosed as sarcoids, but results of histopathological examinations did not always corroborate the clinical diagnosis. Although all the samples were positive for the presence of BPV DNA, after qPCR-HRM analysis 6 (16%) specimens were recognized as BPV-1 “wild”, 24 (63%) as BPV-1 “European” and 8 (21%) as a “variant” of BPV E5 ORF partial CDS. Phylogenetic analysis based on nucleotide sequences of E2 ORF partial CDS and E5 ORF complete CDS was conducted on 7 specimens, whose sequences were published in GenBank and recognized as: 2PL (Accession Number - Acc. No. KC684939) - “variant” BPV-1, 7aPL (Acc. No. KC684940) - “European” BPV-1, 10PL (Acc. No. KC693480) - “variant” BPV-1, 16PL (Acc. No. KC693484) - “variant” BPV-2, 17PL (Acc. No. KC693481) - “variant” BPV-1, 20aPL (Acc. No. KC693482) - “European” BPV-1 and 20cPL (Acc. No. KC693483) - “wild” BPV-1. Amino acid (aa) sequences of E5 ORF complete CDS were also analyzed. The E5 variant of aa sequences found in isolate 10PL (protein identification - ID: AGM 20700) is a novel variant of E5 ORF complete CDS of BPV-1 detected in equine sarcoid in Poland.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 2; 239-245
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phylogenetic analysis of bovine papillomavirus E5 detected in equine sarcoids in Poland
Autorzy:
Szczerba-Turek, A.
Siemionek, J.
Bancerz-Kisiel, A.
Ras, A.
Szweda, W.
Powiązania:
https://bibliotekanauki.pl/articles/30859.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the study was to analyse a part of the sequence of the E5 gene of bovine papil-lomaviruses (BPV) associated with equine sarcoids in Polish horses. Samples of 40 skin lesions obtained from 29 horses were collected for molecular examination. The PCR amplicons of BPV DNA were detected in 38 specimens. After phylogenetic analysis 37 specimens were recognized as BPV-1 and one as BPV-2. Phylogenetic analysis has allowed the classification of the amplicons into two phylogenetic groups (A1,) and four separate isolates (2, 10, 16, 17).
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Neoplastic lesions in slaughter animals in Warminsko-Mazurskie voivodship (Poland) area during the years 2001-2007
Autorzy:
Radkowski, M.
Siemionek, J.
Zdrodowska, B.
Powiązania:
https://bibliotekanauki.pl/articles/30241.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
slaughter animal
Polska
Warmia-Mazury voivodship
cattle
pig
post-slaughter examination
Polish Veterinary Inspection
animal disease
oncology
cancer
Opis:
During the period of 7 years, 2001-2007, 110,273 heads of cattle and 3,055,654 heads of pigs were examined by veterinarians of the Polish Veterinary Inspection in Warmińsko-Mazurskie voivodship. As a result of pre- and post-slaughter examination, 311 heads of cattle and 23 heads of pigs representing 0.279% and 0.00075% of the population respectively were considered unsuitable due to neoplastic lesions. The most frequently found lesions in cattle were fibroneuromas, neuromas and liposarcomas while in case of pigs those were various types of sarcomas, fibromas, cancers, nephromas and melanomas.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic evaluation of bovine papillomavirus types detected in equine sarcoids in Poland
Autorzy:
Szczerba-Turek, A.
Siemionek, J.
Ras, A.
Bancerz-Kisiel, A.
Platt-Samoraj, A.
Lipczynska-Ilczuk, K.
Szweda, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087581.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bovine papillomavirus
equine sarcoid
disease ecology
FAP59/FAP64
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 25-29
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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