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Wyszukujesz frazę "Shams-Bakhsh, M." wg kryterium: Autor

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    Wyświetlanie 1-3 z 3
    Tytuł:
    Identification of viruses infecting cucurbits and determination of genetic diversity of cucumber mosaic virus in Lorestan province, Iran
    Autorzy:
    Hasanvand, V.
    Shams-bakhsh, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/66238.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    identification
    virus
    cucurbit
    determination
    genetic diversity
    cucumber mosaic virus
    RFLP analysis
    Lorestan province
    Iran
    Opis:
    Various viral pathogens infect Cucurbitaceae and cause economic losses. Th e aim of the present study was to detect plant viral pathogens including Cucumber mosaic virus (CMV), Cucumber green mottle mosaic virus (CGMMV), Zucchini yellow mosaic virus (ZYMV), Cucurbit yellow stunting disorder virus (CYSDV) and Cucurbit chlorotic yellows virus (CCYV) in Lorestan province, in western Iran, and also to determine CMV genetic diversity in Iranian populations. A total of 569 symptomatic leaf samples were collected in 2013 and 2014 from cucurbits growing regions in Lorestan province. Th e collected samples were assessed for viral diseases by ELISA. Th e results showed virus incidences in most regions. Th en, the infection of 40 samples to CMV was confi rmed by RT-PCR. Moreover, to distinguish between the two groups (I and II) of CMV, PCR products were digested by two restriction enzymes XhoI and EcoRI. Results of the digestion showed that the isolates of Lorestan belonged to group I. Th e CMV-coat protein gene of eight isolates from diff erent regions and hosts was sequenced and phylogenetic analysis was performed. Subsequent analyses showed even more genetic variation among Lorestan isolates. Th e phylogenetic tree revealed that Lorestan province isolates belonged to two IA and IB subgroups and could be classifi ed together with East Azerbaijan province isolates. Th e results of the present study indicate a wide distribution of CMV, ZYMV, CGMMV, CYSDV and CCYV viruses in cucurbits fi elds of Lorestan province and for the fi rst time subgroup IB of CMV was reported on melon from Iran.
    Źródło:
    Journal of Plant Protection Research; 2017, 57, 2
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence and phylogenetic analysis of Fig mosaic virus and Fig badnavirus-1 in Iran
    Autorzy:
    Alimoradian, M.
    Rakhshandehroo, F.
    Shams-bakhsh, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/66369.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    prevalence
    phylogenetic analysis
    Fig mosaic virus
    Fig badnavirus-1
    Iran
    fig tree
    plant disease
    Opis:
    Fig mosaic virus (FMV) and Fig badnavirus-1 (FBV-1) are two of the most important fig infecting viruses. The incidence and distribution of FBV-1 and FMV were determined by testing in PCR 138 asymptomatic and symptomatic samples. These samples were collected from 60 fig gardens and agricultural fields in three provinces of Iran. The fig infecting viruses FBV-1 and FMV, respectively, were detected in 92 (66.6%) and 34 (24.6%) samples collected from all the surveyed fields. Overall, 24 out of 138 (17.3%) samples showed mixed infections. The sequence analysis of a genomic fragment of 922 nt, comprising the entire ORF-2 and part of the 5’ termini of the ORF-3 of 10 selected FBV-1 Iranian isolates from different provinces, and of the type member from GenBank (Acc. No: JF411989), showed a variation ranging from 1 to 3% at nucleotide level and 1% at the amino acid level. The phylogenetic analysis grouped the FBV-1 isolates into two groups, with the Iranian isolates clustered in two distinct subgroups of group I, according to their geographical origin. In our research, the prevalence and sequence analysis of FBV-1 as the only identified DNA virus infecting fig trees, was studied for the first time in Iran.
    Źródło:
    Journal of Plant Protection Research; 2016, 56, 2
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic diversity among Xanthomonas citri subsp. citri strains in Iran
    Autorzy:
    Rezaei, M.K.
    Shams-Bakhsh, M.
    Alizadeh, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/65267.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Citrus bacterial canker (CBC) is one of the most important diseases of citrus. It is caused by Xanthomonas citri subsp. citri (Xcc). To investigate the variability of Xcc, a collection of twenty-five strains were isolated from the Fars, Hormozgan, Kerman and Sistan-va-Baluchestan provinces of Iran. The twenty-five strains were assessed phenotypically and genetically. These strains had similar biochemical properties. Based on host range determination, the strains were divided into two groups; the first group was pathogenic on Mexican lime (Citrus aurantifolia), citrumelo (Poncirus trifoliata x C. paradisi), citrange (C. sinensis x P. trifoliata) and sour orange (C. aurantium) varieties. The second group was pathogenic on Mexican lime only. Profile of cellular soluble proteins analyzed by sodium dodecyl sulphate-polyacryamid gel electrophoresis (SDS-PAGE) did not reveal any considerable differences among strains. Genetic diversity analyses were performed using two marker systems; repetitive polymerase chain reaction (rep-PCR) and random amplified polymorphic DNA (RAPD). The results of this research showed that two primers, ERIC 1R and 232, with the highest marker index, resulted in the most genetic variability among strains. Cluster analysis by band patterns showed that strains from the Sistan-va-Baluchestan province were a different group, so it was concluded that geographical origin of strains from the Sistan-va-Baluchestan province is different than the geographical origin of strains isolated from other provinces.
    Źródło:
    Journal of Plant Protection Research; 2012, 52, 1
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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