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    Wyświetlanie 1-14 z 14
    Tytuł:
    Potential sources of infection with selected zoonotic agents in the veterinary work environment - pilot studies
    Autorzy:
    Wójcik-Fatla, A.
    Sroka, J.
    Zając, V.
    Sawczyn-Domańska, A.
    Kloc, A.
    Zwoliński, J.
    Kłapeć, T.
    Studzińska, M.B.
    Chmura, R.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085448.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    environment
    Coxiella burnetii
    Toxoplasma gondii
    veterinarians
    Leptospira spp
    Cryptosporidium spp
    Giardia duodenalis
    Opis:
    Introduction and Objective. The problem of occupational biohazards is very important, especially in the field of agriculture and in human and veterinary medicine. The aim of the study was to determine the potential sources of infection in veterinary professionals with selected zoonotic agents, including: Toxoplasma gondii, Giardia duodenalis, Leptospira spp., Cryptosporidium spp. and Coxiella burnetii. Materials and Method. A total of 50 air samples from barns, piggeries and veterinary surgeries were examined for the presence of Leptospira spp. and C. burnetii DNA. Serum samples of 86 pigs and 80 cows were tested for the presence of antibodies to Leptospira spp. and to phase I and II C. burnetii antigens. Serum of 70 cats were tested for the presence of antibodies to T. gondii and 65 samples of cat faeces for the presence of T. gondii oocysts. The presence of G. duodenalis and Cryptosporidium spp. were examined in 50 of dog faeces and 50 of bovine faeces samples. Results. DNA of Leptospira spp. was detected in 2 air samples from the piggeries (4%). C. burnetii DNA was not found in any sample. Anti-Leptospira spp. antibodies were detected in 51 (59.3%) of examined pigs. Neither anti-Leptospira spp. nor anti-C. burnetii antibodies were found among samples of bovine serum. Anti-T. gondii antibodies was found in 52 cat serum samples (74.3%). Among samples of cat faeces, no T. gondii oocysts were detected. In one sample of cattle stool (2%), G. duodenalis was detected and in another (2%) – Cryptosporidium spp. G. duodenalis was detected in 7 samples (14%) and Cryptosporidium spp. in 2 samples (2%) of dog faeces. Conclusions. The results of this study demonstrate the potential risk of infection with Leptospira spp. in veterinarians working with pigs. Veterinarians could be also be at risk of infection with T. gondii and G. duodenalis.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 1; 146-150
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Psychological factors and genetic characteristics of rural cannabis users
    Autorzy:
    Lachowicz, M.
    Chmielowiec, J.
    Chmielowiec, K.
    Suchanecka, A.
    Michałowska-Sawczyn, M.
    Mierzecki, A.
    Mroczek, B.
    Grzywacz, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085700.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    addiction
    polymorphism
    personality traits
    cannabis
    Opis:
    Introduction. Marijuana is one of the most widely used psychoactive substance. There is evidence of genetic predisposition for addiction. Objective. The aim of the study is to evaluate personality traits measured by the NEO Five-Factor Inventory and State-Trait Anxiety Inventory, combined with analysis of Tag1B rs1079597 and Tag1D rs1800498 located in the DRD2 gene. Materials and method. The study group consisted of 214 rural cannabinoid users and 301 controls. The same psychometric test and real-time PCR genotyping were performed in both studied groups. Results. The values of Anxiety state, Anxiety trait, NEO FFI: Neuroticism and Openness in the rural cannabis using group were significantly higher than in the control group. On the other hand, lower values were observed among rural people using cannabis compared to the control group for NEO FFI: Extraversion, Agreeability and Conscientiousness. In the Anxiety trait subscale, a 2% association with the polymorphism DRD2 Tag1B rs1079597 was detected in subjects using cannabis. However, for the DRD2 Tag1D rs1800498, there was no effect on the differences in personality traits between rural cannabis users and the control group. Conclusions. The study shows differences in personality traits between the cannabis using group and controls. Interaction between genetic factors and personality traits was also detected. The association showing the combination of psychological characteristics and genetic variants can bring us closer to the overall picture of the issue of marijuana addiction.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 2; 260-268
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Significant association of DRD2 and ANKK1 genes with rural heroin dependence and relapse in men
    Autorzy:
    Lachowicz, M.
    Chmielowiec, J.
    Chmielowiec, K.
    Suchanecka, A.
    Masiak, J.
    Michałowska-Sawczyn, M.
    Mroczek, B.
    Mierzecki, A.
    Ciechanowicz, I.
    Gezywacz, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085710.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Addiction
    heroin
    dopamine
    haplotypes
    Opis:
    Introduction. Substance abuse significantly influences human health and may induce problems with social functioning worldwide. Numerous genetic and environmental risk factors, as well as their interactions, accelerate the development of drug addiction. Etiologically, the dopaminergic mesocorticolimbic reward pathways are related to psychoactive substance addiction, and the reward properties of heroin are connected with changes in the mesolimbic dopaminergic system. Objective. The aim of this study is a haplotypic analysis of subjects addicted to polysubstance. However, with the knowledge that this is not a homogenous subgroup, it was decided to separate and analyze homogenous subgroups of subjects in order to find specific haplotypic variants among them. The subjects in the subgroups were addicted to heroin, and subjects with more than two relapses in the past two years. Materials and method. The study group comprised of 301 polysubstance addicted rural male subjects. From this group, 2 homogenous subgroups of subjects were isolated and additionally analyzed: (1) a group of heroin addicted subjects (n=61), and (2) a group of heroin-addicted subjects with at least two relapses in the last two years (n=21). The group consisting of all polysubstance addicted rural subjects and both homogenous subgroups were analyzed against a control group of non-addicted subjects (n=300), matching gender and age. Five polymorphisms in the DRD2/ANKK1 region were analyzed: rs1076560, rs1800498, rs1079597, rs6276 in the DRD2 gene, and rs1800497 in the ANKK1 gene. Results. A statistically significant haplotype association was found in analysis of the heroin addicted subjects, compared to controls, and two possible trends – when comparing the whole group of addicted subjects to controls, and in relapse subgroups, compared to the controls. Conclusion. The results obtained showed that haplotypes indicate a part of the biological component of addiction.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 2; 269-273
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part II – pathogenesis
    Autorzy:
    Dutkiewicz, J.
    Zając, V.
    Sroka, J.
    Wasiński, B.
    Cisak, E.
    Sawczyn, A.
    Kloc, A.
    Wojcik-Fatla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081593.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Streptococcus suis
    pathogenesis
    infection stages
    interaction with host cells and tissues
    virulence factors
    genomic
    determinants of pathogenicity
    biofilm formation
    Opis:
    Abstract Streptococcus suis is a re-emerging zoonotic pathogen that may cause severe disease, mostly meningitis, in pigs and in humans having occupational contact with pigs and pork, such as farmers, slaughterhose workers and butchers. The first stage of the pathogenic process, similar in pigs and humans, is adherence to and colonisation of mucosal and/or epithelial surface(s) of the host. The second stage is invasion into deeper tissue and extracellular translocation of bacterium in the bloodstream, either free in circulation or attached to the surface of monocytes. If S. suis present in blood fails to cause fatal septicaemia, it is able to progress into the third stage comprising penetration into host’s organs, mostly by crossing the blood-brain barrier and/or blood–cerebrospinal fluid barrier to gain access to the central nervous system (CNS) and cause meningitis. The fourth stage is inflammation that plays a key role in the pathogenesis of both systemic and CNS infections caused by S. suis. The pathogen may induce the overproduction of pro-inflammatory cytokines that cause septic shock and/or the recruitment and activation of different leukocyte populations, causing acute inflammation of the CNS. Streptococcus suis can also evoke – through activation of microglial cells, astrocytes and possibly other cell types – a fulminant inflammatory reaction of the brain which leads to intracranial complications, including brain oedema, increased intracranial pressure, cerebrovascular insults, and deafness, as a result of cochlear sepsis. In all stages of the pathogenic process, S. suis interacts with many types of immunocompetent host’s cells, such as polymorphonuclear leukocytes, mononuclear macrophages, lymphocytes, dendritic cells and microglia, using a range of versatile virulence factors for evasion of the innate and adaptive immune defence of the host, and for overcoming environmental stress. It is estimated that S. suis produces more than 100 different virulence factors that could be classified into 4 groups: surface components or secreted elements, enzymes, transcription factors or regulatory systems and transporter factors or secretion systems. A major virulence factor is capsular polysaccharide (CPS) that protects bacteria from phagocytosis. However, it hampers adhesion to and invasion of host’s cells, release of inflammatory cytokines and formation of the resistant biofilm which, in many cases, is vital for the persistence of bacteria. It has been demonstrated that the arising by mutation unencapsulated S. suis clones, which are more successful in penetration to and propagation within the host’s cells, may coexist in the organism of a single host together with those that are encapsulated. Both ‘complementary’ clones assist each other in the successful colonization of host’s tissues and persistence therein. S. suis has an open pan-genome characterized by a frequent gene transfer and a large diversity. Of the genetic determinants of S. suis pathogenicity, the most important are pathogenicity islands (PAI), in particular, a novel DNA segment of 89 kb length with evident pathogenic traits that has been designated as 89K PAI. It has been estimated that more than one-third of the S. suis virulence factors is associated with this PAI. It has been proved that the virulent S. suis strains possess smaller genomes, compared to avirulent ones, but more genes associated with virulence. Overall, the evolution of the species most probably aims towards increased pathogenicity, and hence the most significant task of the current research is an elaboration of a vaccine, efficient both for humans and pigs.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 1; 186-203
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena narażenia populacji wiejskiej Lubelszczyzny na zakażenie krętkami z rodzaju Leptospira, ze szczególnym uwzględnieniem terenów popowodziowych
    Assessment of risk of infection with Leptospira spirochetes among rural population in the Lublin Region, with particular consideration of areas exposed to flooding
    Autorzy:
    Dutkiewicz, J.
    Wojcik-Fatla, A.
    Zajac, V.
    Wasinski, B.
    Knap, P.J.
    Sroka, J.
    Cisak, E.
    Sawczyn, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/4449.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Medycyny Wsi
    Opis:
    Wprowadzenie. Leptospiroza, wywoływana przez krętki z rodzaju Leptospira, jest uważana za najbardziej rozprzestrzenioną chorobę odzwierzęcą na świecie. Zapadalność może być zwiększona przez klęski żywiołowe takie jak powodzie i tajfuny. Cel pracy. Celem pracy było zbadanie sytuacji epidemiologicznej leptospirozy na Lubelszczyźnie (wschodnia Polska), z uwzględnieniem wpływu powodzi Wisły, poprzez badania populacji ludzkiej i różnych elementów środowiska na obecność Leptospira w dwóch rejonach: rejonie „A” nawiedzanym przez powodzie i rejonie „B” nienawiedzanym przez powodzie. Materiał i metody. W rejonach „A” i „B” przebadano surowice, odpowiednio 100 i 98 mieszkańców, surowice 32 i 41 świń oraz surowice 41 i 40 krów – na obecność przeciwciał anty- Leptospira, wykorzystując test aglutynacji mikroskopowej (MAT). We wskazanych rejonach przebadano również, odpowiednio 40 i 64 próbki wody, 40 i 68 próbek gleby, próbki organów 30 i 30 drobnych ssaków należących do 5 i 6 gatunków, a także 540 i 296 kleszczy Ixodes ricinus – na obecność DNA Leptospira; badanie przeprowadzono testem nested-PCR. Wyniki. Obecność przeciwciał anty-Leptospira stwierdzono u 3% mieszkańców rejonu „A” i u 9,2% mieszkańców rejonu „B”; różnica ta nie była statystycznie znamienna. Częstość występowania przeciwciał anty-Leptospira była większa u świń i krów z rejonu „A” w porównaniu z rejonem „B” (odpowiednio 34,4% wobec 4,9%, i 26,8% wobec 15,0%), a w przypadku świń różnica okazała się znamienna (P=0,0015). Także miana dodatnich reakcji były wyższe w rejonie „A” w porównaniu z „B”, a w przypadku krów różnica okazała się znamienna (P=0,0128). Obecność DNA Leptospira spp. stwierdzono u 20% drobnych ssaków z rejonu „A” i u 30% z rejonu „B”; różnica ta nie była znamienna, chociaż częstość dodatnich wyników była w obu przypadkach wysoka w porównaniu z danymi z piśmiennictwa. Przeważającą część wyników dodatnich uzyskano u myszy polnych (Apodemus agrarius). Obecność DNA Leptospira spp. wykryto u 15,6% kleszczy I. ricinus z rejonu „A” w porównaniu z 1,4% w rejonie „B”, a różnica ta okazała się wysoce znamienna (P<0,0001). DNA Leptospira spp. zawierało 5% próbek wody z rejonu „A”, podczas gdy wszystkie próbki wody z rejonu „B” oraz wszystkie próbki gleby z rejonów „A” i „B” były ujemne. Wnioski. • Zakażenia krętkami Leptospira występujące u ludności wiejskiej zamieszkującej tereny Lubelszczyzny nawiedzane przez powodzie Wisły nie są częstsze w porównaniu z ludnością zamieszkującą inne części regionu nienawiedzane przez powodzie. • Potencjalnym źródłem zakażeń leptospirami na terenach nawiedzanych przez powodzie są zwierzęta: kleszcze Ixodes ricinus, zwierzęta hodowlane (świnie, krowy) i myszy polne (Apodemus agrarius). • Wykrycie DNA Leptospira spp. w 5% próbek wody z terenów popowodziowych, przy braku pozytywnych wyników z terenu kontrolnego, wydaje się potwierdzać możliwą rolę wody w szerzeniu leptospirozy. • Gleba nie stanowi istotnego zagrożenia na terenach nawiedzanych przez powodzie.
    Background. Leptospirosis, caused by Leptospira spirochetes, is considered the most widespread zoonosis worldwide. Morbidity may be increased by natural disasters such as floods or typhoons. Aim of the study. The aim of the present study was to investigate the epidemiological situation of leptospirosis in the Lublin Region (Eastern Poland) with consideration of the effects of flooding by the Vistula River, by investigating human population and various elements of the environment for the presence of Leptospira in two areas: ‘Area A’ exposed to flooding, and ‘Area B’ not exposed to flooding. Material and Methods. In the Areas A and B, sera of 100 and 98 inhabitants, sera of 32 and 41 pigs and sera of 41 and 40 cows were examined, respectively, for the presence of anti-Leptospira antibodies by the microscopic agglutination test (MAT), as well as 40 and 64 samples of water, 40 and 68 samples of soil, organ samples of 30 and 30 small mammals from 5 and 6 species, and 540 and 296 Ixodes ricinus ticks, respectively – for the presence of Leptospira DNA by the nested-PCR test. Results. The presence of anti-Leptospira antibodies was found in 3% of inhabitants of Area A and in 9.2% of Area B; this difference was statistically insignificant. The frequency of anti-Leptospira antibodies was higher in pigs and cows from Area A, compared to area B (34.4% vs. 4.9%, and 26.8% vs. 15.0%, respectively), while in the case of pigs the difference was significant (P=0.0015). Also, the titers of positive reactions were higher in Area A, compared to Area B, and for cows the difference was significant (P=0.0128). The presence of Leptospira DNA was found in 20% of small mammals from Area A, and in 30% from Area B; this difference being insignificant; however, in both cases the frequency of positive results was high, compared to the data from literature. The great majority of positive results were obtained in striped field mice (Apodemus agrarius). The presence of Leptospira DNA was detected in 15.6% of I. ricinus ticks from Area A, compared to 1.4% in Area B, and the difference was highly significant (P<0.0001). 5% of water samples from Area A contained Leptospira DNA, whereas all water samples from Area B, and all soil samples from Areas A and B were negative. Conclusions. • The infections with Leptospira spirochetes among the rural population inhabiting the areas of the Lublin Region exposed to floods by the Vistula River do not occur with a higher frequency, compared to the areas not exposed to floods. • The potential sources of infection with Leptospira in the areas exposed to flooding are animals: ticks Ixodes ricinus, domestic animals (pigs, cows), and striped field mice (Apodemus agrarius). • Detection of Leptospira spp. DNA in 5% of water samples in the areas exposed to floods, with negative results in control areas, seems to confirm a possible role of water in spreading leptospirosis. • Soil does not constitute any significant risk of infection in the areas exposed to flooding.
    Źródło:
    Medycyna Ogólna i Nauki o Zdrowiu; 2015, 21(50), 1
    2083-4543
    Pojawia się w:
    Medycyna Ogólna i Nauki o Zdrowiu
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurrence of Leptospira DNA in water and soil samples collected in eastern Poland
    Autorzy:
    Wojcik-Fatla, A.
    Zajac, V.
    Wasinski, B.
    Sroka, J.
    Cisak, E.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51299.pdf
    Data publikacji:
    2014
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2014, 21, 4
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A small-scale survey of hantavirus in mammals from eastern Poland
    Autorzy:
    Wojcik-Fatla, A.
    Zajac, V.
    Knap, J.P.
    Sroka, J.
    Cisak, E.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51577.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    hantavirus
    Bunyaviridae
    mammal
    small mammal
    Microtus agrestis
    Myodes glareolus
    Sorex araneus
    epidemiology
    polymerase chain reaction
    flood
    Polska
    Opis:
    Samples of 30 dead small mammals each were collected on area ‘A’ located in eastern Poland which is exposed to flooding by the Vistula river, and on the area ‘B’, also located in eastern Poland but not exposed to flooding. Kidneys and livers of the mammals were examined by the PCR and nested PCR methods for the presence of hantavirus RNA. Out of 7 species of small mammals examined, the presence of hantaviruses was detected in 4 of them. Hantavirus prevalence was low in Apodemus agrarius (2.6%), the most numerous mammal species, whereas in the remaining 3 positive species (Microtus agrestis, Myodes glareolus, Sorex araneus) this was 12.5–100%. The presence of hantaviruses was detected only in the animals found on area ‘A’ exposed to flooding, and their prevalence was statistically greater compared to area ‘B’ not exposed to flooding (16.7% vs. 0%, p=0.0345). The overall positivity of the examined small mammals population from the areas ‘A’ and ‘B’ was 8.3%. The sequence analysis of the samples positive for hantavirus proved that the amplified products showed 77–86% homology with the L segment sequence of hantavirus Fusong-Mf-731 isolated from Microtus fortis in China. The presented study is the first to demonstrate the occurrence of hantavirus infection in small mammals from eastern Poland, and the first to demonstrate the significant relationship between flooding and the prevalence of hantaviruses in small mammals.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on tick-borne rickettsiae in eastern Poland. I. Prevalence in Dermacentor reticulatus (Acari: Amblyommidae)
    Autorzy:
    Wojcik-Fatla, A.
    Cisak, E.
    Zajac, V.
    Sroka, J.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50634.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Rickettsia
    Rickettsia raoultii
    Polska
    prevalence
    Dermacentor reticulatus
    tick
    Acari
    Amblyommidae
    Opis:
    Rickettsia spp. transmitted by ticks are classified mostly in the Spotted Fever Group Rickettsiae (SFGR). Numerous species of this group have been identified in Eurasia as emerging pathogens, but still little is known about their occurrence, effects on human health, and co-incidence with other tick-borne pathogens. The aim of the presented study was to determine the prevalence of Rickettsia spp. in adult Dermacentor reticulatus (Acari: Amblyommidae) ticks collected in Lublin province of eastern Poland using the PCR method. The infection rate of D. reticulatus with Rickettsia spp. was 53.0%. All except one rickettsial isolates showed 100% homology with Rickettsia raoultii. A high prevalence of R. raoultii in Dermacentor reticulatus ticks from eastern Poland suggests that the SFGR species should be considered as potential causative agents of tick-borne diseases in this area.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on tick-borne rickettsiae in eastern Poland. II. Serological response of occupationally exposed populations
    Autorzy:
    Zajac, V.
    Wojcik-Fatla, A.
    Cisak, E.
    Sroka, J.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51448.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    tick
    Rickettsia
    Polska
    serological response
    occupational exposure
    population
    serology
    ELISA test
    forestry worker
    agricultural worker
    Opis:
    A group of 150 persons living in the Lublin province of eastern Poland and occupationally exposed to tick bite were examined by the immunoenzymatic ELISA test for the presence of antibodies against tick-borne Spotted Fever Group (SFG) rickettsiae. The group consisted of 75 forestry workers employed in 3 forest inspectorates and 75 agricultural workers living in 2 villages. As a control group, 43 urban dwellers living in the city of Lublin and not occupationally exposed to tick bite were examined. Among 150 persons occupationally exposed to tick bite, the presence of antibodies against SFG rickettsiae was found in 54 (36.0% of the total). In the control group, the frequency of positive findings was only 4.7%, being significantly smaller compared to the exposed group (p=0.0001). Within the exposed group, the percentage of positive results in forestry workers (50.7%) was greater than in agricultural workers (21.3%); the difference was statistically significant (p=0.0002). Also within this group, the frequency of positive findings in males(46.5%) was significantly greater than in females (21.9%) (p=0.0029). In the exposed group, the positive results tended to increase with the age of the examined persons. However, a significant relationship between age and positive findings was found only in forestry workers (c2=14.207, p=0.00264), but not in agricultural workers and total exposed workers. The frequencies of positive results in forestry workers varied significantly depending on place of work (c2=11.271, p=0.00357). Similarly, the difference between the positive reactions in agricultural workers living in 2 villages proved to be significant (34.2% vs. 8.1%; p=0.0074). The obtained results indicate that people occupationally exposed to tick bite and living in the area of eastern Poland where over half of Dermacentor reticulatus ticks harbour SFG rickettsiae, are under significantly increased risk of infection with these rickettsiae.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The use of a geochemical method in the detection of groundwater discharge to floodplain lakes
    Zastosowanie metody geochemicznej do identyfikacji zasilania podziemnego starorzeczy
    Autorzy:
    Glinska-Lewczuk, K.
    Burandt, P.
    Banaszek, Z.
    Chormanski, J.
    Slapinska, M.
    Timofte, C.
    Serwotka, L.
    Sawczyn, J.
    Jarzab, N.
    Powiązania:
    https://bibliotekanauki.pl/articles/84954.pdf
    Data publikacji:
    2013
    Wydawca:
    Akademia Pomorska w Słupsku
    Tematy:
    geochemical method
    detection
    ground water
    discharge
    flood plain lake
    oxbow lake
    hydrological connectivity
    thermal gradient
    aeration
    Opis:
    Water quality parameters of floodplain lakes may be indicative of the intensity of groundwater recharge. The main assumption made in the study is that the direct influence of groundwater recharge is reflected in the vertical gradient of temperature and aeration along the whole water column. Considering this, we seasonally monitored physical and chemical properties of 22 oxbow lakes in postglacial river valleys (the Słupia, Drwęca and Łyna rivers) in temperate climate zone in the southern watershed of the Baltic Sea (N Poland). The results were compared with groundwater samples from transects of piezometers located near the floodplain lakes. The floodplain water bodies showed variability (both in vertical and spatial dimensions) in temperature, aeration and electrolytic conductivity, affected mainly by different sources of water supply. The temperatures and dissolved oxygen contents declined not only with the increasing depth of water and a distance from the river channel, but also a significant drop in the parameters’ values have been associated with groundwater recharge within the floodplain edge.
    Parametry jakości wód w jeziorach rzecznych mogą być traktowane jako indykatory intensywności zasilania podziemnego. Głównym założeniem prowadzonych badań był bezpośredni wpływ zasilania podziemnego na jakość wód starorzeczy zidentyfikowany na podstawie gradientu temperatury i natlenienia wody w przekrojach pionowych i podłużnych zbiorników. Uwzględniając tę hipotezę, monitoringowi poddano właściwości fizyczne i chemiczne wody w 22 starorzeczach północnej Polski (w dolinie rzeki Słupi, Drwęcy i Łyny) położonych w umiarkowanej strefie klimatycznej, w warunkach fizyczno-geograficznych południowego zlewiska Bałtyku. Wyniki uzyskane z monitoringu wód starorzeczy porównano z jakością wód gruntowych badanych w piezometrach położonych w pobliżu jezior rzecznych. Badane zbiorniki charakteryzowały się dużą zmiennością cech fizykochemicznych, głównie natlenienia, przewodności elektrolitycznej wynikającej z różnych źródeł pochodzenia wody. Badania wykazały, że zarówno temperatura, jak i koncentracje tlenu rozpuszczonego zmniejszają się nie tylko wraz ze wzrostem głębokości zbiorników i odległości względem rzeki, ale także znaczący spadek tych wskaźników związany jest z zasilaniem gruntowym u podnóży krawędzi dolin rzecznych.
    Źródło:
    Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline; 2013, 17
    1643-0115
    Pojawia się w:
    Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Leptospirosis as a tick-borne disease? Detection of Leptospira spp. in Ixodes ricinus ticks in eastern Poland
    Autorzy:
    Wojcik-Fatla, A.
    Zajac, V.
    Cisak, E.
    Sroka, J.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51864.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2012, 19, 4
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Seroprevalence of leptospirosis in rural populations inhabiting areas exposed andnot exposed to floods in eastern Poland
    Autorzy:
    Wasinski, B.
    Sroka, J.
    Wojcik-Fatla, A.
    Zajac, V.
    Cisak, E.
    Knap, J.P.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50328.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Medycyny Wsi
    Opis:
    Blood serum samples collected from randomly selected groups of 100 persons inhabiting rural community ‘A’ located in eastern Poland and exposed to floods by the Vistula river, and 98 persons inhabiting rural community ‘B’, also located in eastern Poland, but in the area not exposed to floods were examined by the microscopic agglutination test (MAT) for the presence of antibodies against 18 Leptospira serovars. Positive results showed 3% of serum samples collected from community ‘A’, while the seroprevalence in community ‘B’ was 9.2%, being insignificantly higher than in community ‘A’. For both examined communities (n=198), a significant positive correlation was found between the prevalence and titer of seropositive response and age of examined people (r=0.145, p=0.042). No significant differences were found between the prevalence of positive reactions in males and females (p>0.05). The reactions with 10 serovars of Leptospira (Australis, Autumnalis, Hebdomadis, Hardjo, Sejroe, Zanoni, Bataviae, Bratislava, Canicola and Grippotyphosa), belonging to 3 species (L. interrogans, L. borgpetersenii, L. kirschneri), were found in the examined communities. From both communities, of 12 persons demonstrating positive results in MAT, 9 showed reaction with one serovar, 2 with two serovars and 1 with three serovars. The highest titers found during the examination did not exceeded 800. In conclusion, our results suggest that there is only a slight, if any, hazard of an leptospirosis epidemic after the flood that afflicted eastern Poland in the year 2010 and the general epidemiological situation of leptospirosis in eastern Poland. Although there does not seem to be any cause for concern, further research is needed.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2012, 19, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wpływ karboplatyny na aktywność peroksydazy glutationu (GSH-Px), reduktazy glutationu (GR) oraz stężenie dialdehydu malonowego (MDA) w mediach hodowlanych ludzkich komórek czerniaka złośliwego linii Me45 in vitro
    In vitro study of the infl uence of carboplatin on glutathione peroxidase (GSH-Px), glutathione reductase (GR) activities and malondialdehyde (MDA) concentration in human malignant melanoma Me45 media cells
    Autorzy:
    Bułdak, Rafał J.
    Polaniak, Renata
    Kukla, Michał
    Kubina, Robert
    Skonieczna, Magdalena
    Bułdak, Magdalena
    Stygar, Dominika
    Sawczyn, Tomasz
    Gowarzewski, Marcin
    Żwirska-Korczala, Krystyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1038289.pdf
    Data publikacji:
    2011
    Wydawca:
    Śląski Uniwersytet Medyczny w Katowicach
    Tematy:
    karboplatyna
    enzymy antyoksydacyjne
    dialdehyd malonowy
    peroksydacja lipidów
    carboplatin
    antioxidant enzyme activities
    malondialdehyde
    lipid peroxidation
    Opis:
    INTRODUCTION Reactive oxygen species (ROS) production in cancer cells usually occurs following anticancer drug exposure such as: etoposide, carboplatin compounds, doxorubicin and holoxan treatment. Normal and malignant cells contain a variety of free radical scavenging systems such as antioxidant enzymes that protect them from the eff ects of drugs that generate oxidative stress inside the cells. MATERIALS AND METHODS The culture of human malignant melanoma Me45 cell line was performed in standard conditions. The colonies were treated with two concentrations of carboplatin: (C1) 10 μg/ml and (C2) 100 μg/ml of the culture medium. The control group were colonies without a cytostatic drug in the culture medium. The medium was collected at 24 hours from addition of the carboplatin preparation and used for determination of activity of the following antioxidative enzymes: glutathione peroxidase (GSH-Px), glutathione reductase (GR), as well as the concentration of the malondialdehyde (MDA). RESULTS After 24 hours in the carboplatin treated group (C1-C2) glutathione peroxidase (GSH-Px) media activity was signifi cantly increased in concentration dependent manner in comparison to the control group (K); (125.2 ± 12.1 IU/l, 99.1 ± 13.3 IU/l vs 91.6 ± 12.1 IU/l, respectively, p < 0.001; p < 0.05). Gluthatione reductase (GR) activity was decreased in carboplatin treated groups (C1) with respect to control group (K); (8.49 ± 0.31 IU/l vs 9.49 ± 0.49 IU/l; p < 0.05). MDA media cells level was almost a 2-fold lower in carboplatin (C1) treated group compared with untreated control group (K); (0.71 ± 0.7 μmol/ml, 1.79 ± 0.15 μmol/ml vs 1.49 ± 0.11 μmol/ml, p < 0.05). CONCLUSION Carboplatin at the concentration of 10 μg/ml leads to an increase antioxidative enzyme activity (GSH-Px) and prevent to lipid peroxidation in malignant melanoma Me45 media cells.
    WSTĘP Wytwarzanie reaktywnych form tlenu (RFT) w komórkach nowotworowych zwykle następuje podczas ekspozycji na leki cytostatyczne, takie jak: karboplatyna, etopozyd, doksorubicyna oraz holoksan. Prawidłowe i nowotworowe komórki mają różne systemy zmiataczy wolnych rodników, w tym: enzymy antyoksydacyjne, które chronią je przed generowanym przez cytostatyki stresem oksydacyjnym we wnętrzu komórek. MATERIAŁ I METODY Hodowla ludzkich komórek czerniaka złośliwego linii Me45 była prowadzona w standardowych warunkach. Kolonie komórek były traktowane karboplatyną w dwóch różnych stężeniach (C1) 10 μg/ml medium oraz (C2) 100 μg/ml medium. Grupę kontrolną stanowiły komórki niezawierające w medium hodowlanym leku cytostatycznego. Medium zbierano po 24 godzinach od podania preparatu, wirowano i w supernatantach oznaczano aktywności badanych enzymów antyoksydacyjnych: peroksydazy glutationu (GSH-Px) i reduktazy glutationu (GR), oraz stężenie dialdehydu malonowego (MDA). WYNIKI Po 24 godzinach w grupie komórek traktowanych karboplatyną (C1 oraz C2) aktywność peroksydazy glutationu (GSH-Px) w medium hodowlanym znacząco wzrosła zależnie od stężenia w porównaniu z grupą kontrolną (K); (12512 ± 12,1 IU/l, 99,1 ± 13,3 IU/l vs 91,6 ± 12,1 IU/l, odpowiednio, p < 0,001; p < 0,05). Aktywność reduktazy glutationu (GR) była obniżona w grupie komórek traktowanych karboplatyną (C1) w odniesieniu do grupy kontrolnej (K); (8,49 ± 0,31 IU/l vs 9,49 ± 0,49 IU/l; p < 0,001). Poziom MDA w medium komórkowym był niemal 2-krotnie niższy w grupie komórek traktowanych karboplatyną w stężeniu 10 μg/ml niż w komórkach grupy kontrolnej (K); 0,71 ± 0,7 μmol/ml vs 1,49 ± 0, 11 μmol/ml, p < 0,05). WNIOSKI Karboplatyna w stężeniu 10 μg/ml zwiększa aktywność enzymu GSH-Px i zapobiega procesowi peroksydacji lipidów w mediach komórek czerniaka złośliwego linii Me45 in vitro.
    Źródło:
    Annales Academiae Medicae Silesiensis; 2011, 65, 3; 7-15
    1734-025X
    Pojawia się w:
    Annales Academiae Medicae Silesiensis
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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