- Tytuł:
- Uptake of photofrin II, a photosensitizer used in photodynamic therapy, by tumour cells in vitro
- Autorzy:
-
Chwiłkowska, Agnieszka
Saczko, Jolanta
Modrzycka, Teresa
Marcinkowska, Anna
Malarska, Anna
Bielewicz, Julita
Patalas, Dorota
Banaś, Teresa - Powiązania:
- https://bibliotekanauki.pl/articles/1043629.pdf
- Data publikacji:
- 2003
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
photofrin II
photodynamic therapy
fluorescence spectra - Opis:
- Photosensitizing dyes are used in fluorescence diagnostics and photodynamic therapy (PDT). These usually hematoporphyrin derivatives (HpD) accumulate preferentially within neoplastic tissues. HpD is a mixture of ether and ester linked porphyrins. Its partially purified form is known under the commercial name of photofrin II (PII). PII emission spectra were studied in a hydrophilic (PBS) and a lipophilic (PC liposomes) environment. Red shift was observed in their emission maxima from 615 nm in buffer solution to 635 nm in lipid. Identical red shift was obtained when the intracellular fluorescence of two cancer cell lines, MCF 7 and Jurkat, incubated with PII was investigated. Thus, intramembrane localization of PII may be suggested. As determined from the total fluorescence intensity, the uptake of PII was only slightly higher for Jurkat than for MCF 7 cells. Nevertheless the kinetics of the uptake was found to be different for both cell lines.
- Źródło:
-
Acta Biochimica Polonica; 2003, 50, 2; 509-513
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł