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Wyświetlanie 1-10 z 13
Tytuł:
Construction of a reporter system for Lactobacillus sp. using the gfpuv gene
Autorzy:
Garbaty, T.
Slonska, A.
Klimuszko, D.
Powiązania:
https://bibliotekanauki.pl/articles/31491.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Lactobacilli are bacteria commonly associated with the gastrointestinal tract of animals and humans. They are able to produce antimicrobial substances such as bacteriocins, lactic acid and hydrogen peroxide, the factors which have been shown to be beneficial for controlling overgrowth of potentially pathogenic bacteria. For this reason lactobacilli are often applied in probiotics. The aim of present study was to construct a reporter strain based on the gfpuv expression system which can bee used as a strain with the ability to colonize the intestinal tract in future experiments.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Neuropatogenność końskiego herpeswirusa typu 1 - aktualne dane
Neuropathogenicity of equine herpesvirus 1 (EHV-1) - a review of recent developments
Autorzy:
Brzezicka, J.
Slonska, A.
Chodkowski, M.
Cymerys, J.
Powiązania:
https://bibliotekanauki.pl/articles/860142.pdf
Data publikacji:
2017
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Źródło:
Życie Weterynaryjne; 2017, 92, 02
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wirusy onkogenne w etiopatogenezie nowotworów zwierząt
Oncogenic viruses in the etiopathogenesis of animal tumors
Autorzy:
Chodkowski, M.
Brzezicka, J.
Golke, A.
Slonska, A.
Cymerys, J.
Powiązania:
https://bibliotekanauki.pl/articles/859740.pdf
Data publikacji:
2017
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
choroby zwierzat
nowotwory
etiopatogeneza
onkogeneza wirusowa
wirusy onkogenne
retrowirusy
papillomawirusy
wirus bialaczki bydla
wirus bialaczki kotow
wirus ptasiej mieloblastozy
wirus nowotworu sutka myszy
poliomawirusy
Źródło:
Życie Weterynaryjne; 2017, 92, 10
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of importin α/β and exportin 1 on equine herpesvirus type 1 (EHV-1) replication in primary murine neurons
Autorzy:
Slonska, A.
Cymerys, J.
Skwarska, J.
Golke, A.
Banbura, M W
Powiązania:
https://bibliotekanauki.pl/articles/31471.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Viruses replicating in the nucleus need to cross the nuclear membrane barrier during infection, therefore disruption of specific nuclear transport pathways is crucial for their replication cycle. In the present study we have investigated the influence of nucleo-cytoplasmic transport inhibitors – ivermectin and leptomycin B, on EHV-1 replication in primary murine neurons. Obtained results suggest that the examined proteins – exportin 1 and importin α/β may participate, but are not required, during EHV-1 infection. Based on these results, it can be assumed that EHV-1 is able to use other receptors for nucleo-cytoplasmic transport.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
First report of the oncolytic effect of EHV-1 on the non-small lung cancer – in vitro studies
Autorzy:
Chodkowski, M.
Słońska, A.
Bartak, M.
Bańbura, M.W.
Cymerys, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087133.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
EHV-1
adenocarcinoma cell line
oncolytic virus
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 607-610
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Equine herpesvirus type 1 affects mitochondrial network morphology and reactive oxygen species generation in equine dermal cell line
Autorzy:
Bartak, M.
Chodkowski, M.
Słońska, A.
Bańbura, M.W.
Cymerys, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087318.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
equine herpesvirus type 1
equine dermal cell line
mitochondria
reactive oxygen species
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 309-312
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA
Autorzy:
Osinska, E.
Golke, A.
Slonska, A.
Cymerys, J.
Banbura, M.W.
Dzieciatkowski, T.
Powiązania:
https://bibliotekanauki.pl/articles/30252.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
rapid detection
equine herpesvirus
real-time polymerase chain reaction
horse
herpesvirus
Gammaherpesvirinae
Rhadinovirus
veterinary virology
Opis:
Equid herpesvirus type 2 (EHV-2) together with equid herpesvirus type 5 are members of Gammaherpesvirinae subfamily, genus Rhadinovirus. EHV-2 is one of major agents causing diseases of horses common worldwide. A possible role of EHV-2 in reactivating latent equid herpesvirus type-1 has been suggested, because reactivation of latent EHV-1 was always accompanied by EHV-2 replication. Variety techniques, including cell culture, PCR and its modifications, have been used to diagnose EHV-2 infections. The aim of this study was to develop, optimize and determine specificity of real-time PCR (qPCR) for EHV-2 DNA detection using HybProbesR chemistry and to evaluate clinical samples with this method. The analytical sensitivity of assay was tested using serial dilutions of viral DNA in range between 70 and 7x105 copies/ml. The limit of detection (LOD) was calculated using probit analysis and was determined as 56 copies/ml. In further studies 20 different clinical samples were tested for the presence of EHV-2. Described in-house qPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high level of sensitivity, accuracy and rapidity provided by the LightCycler 2.0 instrument are favorable for the use of this system in the detection of EHV-2 DNA in veterinary virology.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection
Autorzy:
Golke, A.
Cymerys, J.
Slonska, A.
Dzieciatkowski, T.
Chmielewska, A.
Tucholska, A.
Banbura, M.W.
Powiązania:
https://bibliotekanauki.pl/articles/30665.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
xCELLigence system
real-time analysis
label-free analysis
neuronal cell response
dermal cell response
cell response
equine herpesvirus 1
animal infection
herpesvirus
neuron
neuronal cell
dermal cell
Opis:
Real-time cell electronic sensing (RT-CES) based on impedance measurements is an emerging technology for analyzing the status of cells in vitro. It allows label-free, real time monitoring of the biological status of cells. The present study was designed to assess dynamic data on the cell processes during equine herpesvirus type 1 (EHV-1) infection of ED (equine dermal) cells and primary murine neuronal cell culture. We have demonstrated that the xCELLigence system with dynamic monitoring can be used as a rapid diagnostic tool both to analyze cellular behavior and to investigate the effect of viral infection.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Equid herpesvirus type 1 (EHV-1) disrupts actin cytoskeleton during productive infection in equine leukocytes
Autorzy:
Drebert, Z.
Golke, A.
Cymerys, J.
Slonska, A.
Chmielewska, A.
Tucholska, A.
Banbura, M.W.
Powiązania:
https://bibliotekanauki.pl/articles/30687.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Equid herpesvirus type 1 (EHV-1) is a prevalent causative agent of equine diseases worldwide. After primary replication in the respiratory epithelium the virus disseminates systemically through a peripheral blood mononuclear cell (PBMC)-associated viraemia. EHV-1 is the only alphaherpes-virus known so far which is capable of establishing latent infection not only in neurons but also in immune system cells (mainly in lymphocytes and macrophages). Since leukocytes are not the target cells for viral replication but are used to transport EHV-1 to the internal organs, the question remains how the virus avoids the immune response and whether it could potentially be associated with virus-induced cytoskeletal rearrangements. Therefore, the aim of this study was to investigate the progress of EHV-1 replication in leukocytes stimulated by phytohemagglutinin and the impact of EHV-1 infection on the actin cytoskeleton. Using the real-time PCR method we evaluated the quantity of viral DNA from samples collected at indicated time points post infection. In order to examine possible changes in actin cytoskeleton organization due to EHV-1 infection, we performed immunofluorescent staining using TRITC-phalloidin conjugate. The results showed that EHV-1 replicates in leukocytes at a restricted level but with the accompaniment of chromatin degradation. Simultaneously, infection with EHV-1 caused disruption of the actin cytoskeleton; this was particularly apparent in further stages of infection. Disruption of the actin cytoskeleton may lead to the limited release of the virus from the cells, but may be also beneficial for the virus, since at the same time it potentially impairs the immune function of leukocytes.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-10 z 13

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