- Tytuł:
- Translational and structural analysis of the shortest legume ENOD40 gene in Lupinus luteus
- Autorzy:
-
Podkowinski, Jan
Zmienko, Agnieszka
Florek, Blazena
Wojciechowski, Pawel
Rybarczyk, Agnieszka
Wrzesinski, Jan
Ciesiolka, Jerzy
Blazewicz, Jacek
Kondorosi, Adam
Crespi, Martin
Legocki, Andrzej - Powiązania:
- https://bibliotekanauki.pl/articles/1040638.pdf
- Data publikacji:
- 2009
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
nitrogen fixation
transcript structure
ENOD40
phylogeny - Opis:
- Two early nodulin 40 (enod40) genes, ENOD40-1, the shortest legume ENOD40 gene, and ENOD40-2, were isolated from Lupinus luteus, a legume with indeterminate nodules. Both genes were expressed at similar levels during symbiosis with nitrogen-fixing bacteria. ENOD40 phylogeny clustered the L. luteus genes with legumes forming determinate nodules and revealed peptide similarities. The ENOD40-1 small ORF A fused to a reporter gene was efficiently expressed in plant cells, indicating that the start codon is recognized for translation. The ENOD40-1 RNA structure predicted based on Pb(II)-induced cleavage and modeling revealed four structurally conserved domains, an absence of domain 4 characteristic for legumes of indeterminate nodules, and interactions between the conserved region I and a region located upstream of domain 6. Domain 2 contains Mg(II) ion binding sites essential for organizing RNA secondary structure. The differences between L. luteus and Glycine max ENOD40 RNA models suggest the possibility of a switch between two structural states of ENOD40 transcript.
- Źródło:
-
Acta Biochimica Polonica; 2009, 56, 1; 89-102
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł