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Wyświetlanie 1-15 z 563
Tytuł:
Fatty Acid Composition and Anticancer Activity of Neutral and Polar Lipids of Pacific Oyster (Crassostrea gigas) Cultured in Khanh Hoa Coast in Vietnam
Autorzy:
Nguyen, Minh V.
Kakooza, Derrick
Do, Thi Hong Tuoi
Tran, Anh P.
Nguyen, Han T.
Tran, Ngoc Q.
Powiązania:
https://bibliotekanauki.pl/articles/31341116.pdf
Data publikacji:
2024-05-24
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
anticancer activity
fatty acid profile
lipid fraction
n3/n6 ratio
thrombogenicity
Opis:
In this study, we investigated the fatty acid composition and anticancer activity of neutral and polar lipid fractions extracted from Crassostrea gigas oysters cultured in Nha Phu Lagoon, Khanh Hoa Coast, harvested during the five months of January, April, May, September, and November. Analysis revealed that saturated fatty acids (SFAs) were the most abundant fatty acids in the neutral lipid fraction, followed by monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs). Conversely, the polar lipid fraction exhibited a different order, with PUFAs being the most abundant, followed by MUFAs and SFAs. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were identified as the most prevalent polyunsaturated fatty acids, while oleic acid and palmitic acid were the predominant monounsaturated and saturated fatty acids, respectively. Notably, the combined content of EPA and DHA in the polar lipid fraction consistently exceeded 30% throughout all five months of analysis. Thrombogenicity index (TI) values ranged from 0.13 to 0.29 for the polar lipid fraction and from 0.6 to 1.1 for the neutral lipid fraction. Moreover, the polar lipid fraction exhibited significantly higher n3/n6 ratios compared to the neutral lipid fraction. The polar lipid fraction exhibited stronger inhibitory effects on the growth of the three cancer cell lines (HepG2, MDA-MB-231, and RD) compared to the neutral lipid fraction. The findings of the present study show that lipids extracted from C. gigas oysters cultured in Khanh Hoa Coast have a weak anticancer activity but may still aid in prevention and treatment of certain cancer types.
Źródło:
Polish Journal of Food and Nutrition Sciences; 2024, 74, 2; 169-176
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A numerical approach in applying panel method for the added mass of a group of sections in fluids
Autorzy:
Zhang, D.
Li, P.
Wang, Q.
Yang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/38695713.pdf
Data publikacji:
2023
Wydawca:
Instytut Podstawowych Problemów Techniki PAN
Tematy:
numerical method
panel method
fluid-structure interaction
confined fluid
fluid mass coefficients
Opis:
This paper firstly expands an efficient numerical methodology developed from the source panel method to the added mass calculation of long column systems in fluid. Structures submerged in fluid are considered as two-dimensional and are discretized into a number of source panels. The influence coefficient matrices and the potential function are then calculated and the fluid forces are calculated by the unsteady Bernoulli equation. Finally, we utilize this present method to calculate the mass coefficients of some typical problems, which effectively verifies its feasibility and accuracy. This method takes into account both applicability and computational efficiency. On the one hand, in contrast to the analytical method which is only applicable to specific cross-sections, this method is applicable to arbitrary boundaries with C0 continuity in mathematics. On the other hand, this method requires less mesh and computation than commercial software. This paper extends the application of the source panel method which is widely used in aerodynamics to provide a reference for added mass calculation problems in engineering.
Źródło:
Archives of Mechanics; 2023, 75, 1-2; 151-168
0373-2029
Pojawia się w:
Archives of Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Method for calculating the individual hydrocarbon composition from the accumulated fractional composition of oil and condensate samples
Metoda obliczania jednostkowego składu węglowodorów na podstawie skumulowanego składu frakcyjnego próbek ropy naftowej i kondensatu
Autorzy:
Karimova, Arifa Q.
Powiązania:
https://bibliotekanauki.pl/articles/31348266.pdf
Data publikacji:
2023
Wydawca:
Instytut Nafty i Gazu - Państwowy Instytut Badawczy
Tematy:
oil
composition
asphaltene-resinous substances
hydrocarbon gases
chemical properties
paraffins
mixtures of pure components
gas condensate system
individual hydrocarbon
Watson characteristic factor
high degree of correlation
ropa
skład
substancje asfaltenowo-żywiczne
gazy węglowodorowe
właściwości chemiczne
parafiny
mieszanina czystych składników
systemy gazowo-kondensatowe
pojedynczy węglowodór
współczynnik Watsona
wysoki stopień korelacji
Opis:
The composition of oils is the main source of information that enables the evaluation and modeling of their physical, thermal and chemical properties (including their critical properties) across a wide range of state parameters. The more comprehensively the composition of oils is analyzed, the more precise information is provided for calculations and problem-solving in the field of oilfield mechanics and related areas, such as the extraction, preparation, transportation, and processing of oils and their fractions. The principal constituents of oils are hydrocarbons. Oils contain hydrocarbons of three homologous series: paraffins, naphthenes and aromas. In oils, there may be hydrocarbons of a mixed composition containing both naphthenic and aromatic rings. In addition to hydrocarbons, oils also contain oxygen, sulfur, nitrogen and other constituents. These compounds are the primary elements of asphaltene-resinous substances (ARS) found in oil. They are characterized by high relative densities (often exceeding 1 g/cm<sup>3</sub>), dark coloration, and relative chemical instability when exposed to elevated temperatures, oxygen, adsorbents, etc. The composition of asphaltene-resinous substances encompasses a wide range of substances. The validity of the proposed technique is justified by the observation that the data of molecular weight, Watson characteristic factor, and experimental density calculated from molar compositions should align on the same surface with a high degree of correlation. In addition, the experimental and calculated values, in terms of molecular composition, of molecular weights should lie on a straight line passing through the origin of coordinates with a slope coefficient equal to unity and exhibit high degree of correlation.
Skład ropy jest głównym źródłem informacji umożliwiającym ocenę i modelowanie jej właściwości fizycznych, termicznych i chemicznych (włącznie z właściwościami krytycznymi) w szerokim zakresie parametrów stanu. Im bardziej dokładnie analizowany jest jej skład, tym dokładniejsze informacje są dostarczane na potrzeby obliczeń i rozwiązywania problemów w dziedzinie mechaniki złóż ropy i powiązanych obszarach, takich jak wydobycie, przygotowanie, transport i przetwarzanie rop i ich frakcji. Głównymi składnikami ropy są węglowodory. Ropa zawiera węglowodory z trzech szeregów homologicznych: parafiny, nafteny i aromaty. W skład ropy mogą wchodzić węglowodory o mieszanym składzie, zawierające zarówno pierścienie naftenowe, jak i aromatyczne. Oprócz węglowodorów, ropa zawiera także tlen, siarkę, azot i inne składniki. Związki te są podstawowymi składnikami substancji asfaltenowo-żywicznych (ARS) występujących w ropie naftowej. Charakteryzują się one wysoką gęstością względną (często przekraczającą 1 g/cm3), ciemnym zabarwieniem i względną niestabilnością chemiczną po ekspozycji na działanie podwyższonych temperatur, tlenu, adsorbentów itp. Skład substancji asfaltenowo-żywicznych obejmuje szeroki zakres substancji. Uzasadnieniem proponowanej techniki jest fakt, że dane dotyczące masy cząsteczkowej, współczynnika Watsona i gęstości eksperymentalnej obliczonej na podstawie składu molowego powinny znajdować się na tej samej powierzchni z wysokim stopniem korelacji. Ponadto, wartości eksperymentalne i obliczone w odniesieniu do składu molekularnego mas cząsteczkowych powinny znajdować się na linii prostej przechodzącej przez początek współrzędnych ze współczynnikiem nachylenia równym jedności i wykazywać wysoki stopień korelacji.
Źródło:
Nafta-Gaz; 2023, 79, 11; 730-735
0867-8871
Pojawia się w:
Nafta-Gaz
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
MGF360-12L of ASFV-SY18 is an immune-evasion protein that inhibits host type I IFN, NF-κB, and JAK/STAT pathways
Autorzy:
Chen, Q.
Wang, X.X.
Jiang, S.W.
Gao, X.T.
Huang, S.Y.
Liang, Y.
Jia, H.
Zhu, H.F.
Powiązania:
https://bibliotekanauki.pl/articles/16647496.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
African swine fever virus
MGF360-12L
type I IFN
NF-κB
JAK/STAT
Opis:
African swine fever virus (ASFV) causes feverous and hemorrhagic disease of domestic pigs and European wild boars with high mortality, yet no commercial vaccine is currently available. Several ASFV strains with natural deletion or gene-targeted knockout of multiple MGF360 and MGF505 genes are attenuated in vitro and in vivo, and can offer full protection against homologous challenge. However, the mechanisms underlying the protection are not fully understood. This study aims to investigate the effects of MGF360-12L of ASFV-SY18 on the cGAS-STING signaling pathway and explore the potential mechanisms. We identified that ASFV-SY18 MGF360-12L could inhibit cGAS-STING, TBK1, or IRF3-5D-stimulated IFN-β expression and ISRE activation. Specifically, MGF360-12L inhibits both the activation of PRD(III-I) in a dose-dependent manner, and suppresses the exogenous expression of TBK1 and IRF3-5D. MGF360-12L could block NF-κB activation induced by overexpression of cGAS-STING, TBK1, IKKβ. Downstream of the IFN-β signaling, MGF360-12L blocks the ISRE promoter activation by reducing total protein level of IRF9. Moreover, MGF360-12L protein can inhibit IFN-β-mediated antiviral effects. In conclusion, our findings suggest that MGF360-12L is a multifunctional immune-evasion protein that inhibits both the expression and effect of IFN-β, which could partially explain the attenuation of relevant gene-deleted ASFV strains, and shed light on the development of efficient ASFV live attenuated vaccines in the future.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 1; 119-130
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteomics profiles reveal the potential roles of proteins involved in chicken macrophages stimulated by Lipopolysaccharide
Autorzy:
Li, S.
Chen, Y.
Xue, W.
Wang, Q.
Huai, Z.
An, C.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/16647586.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
chicken macrophage (HD11)
immune response
lipopolysaccharide (LPS)
proteomics
Opis:
Lipopolysaccharide (LPS), a core part of gram-negative bacteria, is crucial for inducing an inflammatory response in living things. In the current study, we used LPS from Salmonella to stimulate chicken macrophages (HD11). Proteomics was used to investigate immune-related proteins and their roles further. Proteomics investigation revealed 31 differential expression proteins (DEPs) after 4 hours of LPS infection. 24 DEPs expressions were up-regulated, while seven were down-regulated. In this investigation, ten DEPs were mainly enriched in S. aureus infection, complement, and coagulation cascades, which were all implicated in the inflammatory response and clearance of foreign pathogens. Notably, complement C3 was shown to be up-regulated in all immune-related pathways, indicating that it is a potential protein in this study. This work contributes to a better understanding and clarification of the processes of Salmonella infection in chickens. It might bring up new possibilities for treating and breeding Salmonella-infected chickens.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 2; 265-274
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Review and future perspectives on the integration characteristics for equine lentivirus in the host genome
Autorzy:
Yu, Y.-Y.
Xu, M.-S.
Liang, H.
Wang, H.-Y.
Yu, C.-Q.
Liu, Q.
Powiązania:
https://bibliotekanauki.pl/articles/16647532.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
lentivirus
equine infectious anemia virus
integration
long interspersed elements
Opis:
Despite over 40 years of research on the human immunodeficiency virus type 1 (HIV-1) vaccine, we still lack a considerable progress. Equine infectious anemia virus (EIAV) is a lentivirus in the Retroviridae family, akin to HIV-1 in genome structure and antigenicity. EIA is an important infectious disease in equids, characterized by anemia, persistent infection, and repeated fevers. The EIAV attenuated vaccine in China is the only lentiviral vaccine used on a large scale. Elucidating the mechanism of waning and induction of protective immunity from this attenuated vaccine strain will provide a critical theoretical basis and reference point for vaccine research, particularly in the development of lentivirus vaccines, with far-reaching scientific value and social significance. In this paper, we summarize the information related to EIAV integration site selection, particularly for the Chinese EIAV attenuated vaccine strains on the equine genome. This may improve our mechanistic understanding of EIAV virulence reduction at the host genome level. The obtained data may help elucidate the biological characteristics of EIAV, particularly the Chinese attenuated EIAV vaccine strain, and provide valuable information regarding retroviral infections, particularly lentiviral infection and associated therapeutic vectors.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 1; 163-172
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative analysis of CpG islands in two genotypes of African swine fever virus
Autorzy:
Yu, Y.-Y.
X, M.-S.
Liu, Q.
Powiązania:
https://bibliotekanauki.pl/articles/16539329.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
African Swine Fever Virus (ASFV)
genotype
DNA methylation
CpG island
Opis:
African swine fever (ASF) is an acute, hemorrhagic, and devastating viral infectious disease that causes important economic losses to the swine industry. Currently, there are no effective vaccines or drugs available. Epigenetic mechanisms, especially cytosine methylation of cytosine-phosphate-guanine (CpG) islands, have a significant impact on the life cycle of several viruses. Hence, drugs targeting DNA methylation may potentially be used for the treatment of ASF. Here, we selected the inner core, core shell, inner membrane, capsid, and external envelope membrane, to analyze the characteristics of CpG islands in the ASF virus (ASFV) genomes. Furthermore, we analyzed the promoters and CpG islands in the upstream regions of these genes. Results showed that the CpG islands of seven genes were conserved in the genomes of two genotype of ASFV strains, whereas the CpG islands of other genes were relatively conserved (ASFV strains differed mainly in the quantity of CpG islands). The different distribution of CpG islands in the genomes of different ASFV strains may affect their methylation status, which may in turn affect the regulation of viral gene expression, leading to different clinical outcomes. In addition, the predicted promoter regions based on the upstream sequences of most genes overlapped with CpG island positions. Methylation of the binding sites of the promoter regions inhibits the binding of the transcription factors to the promoters, thus inhibiting the activation of the promoters and limiting the synthesis of viral proteins. The results of this study provide a basis for exploring new antiviral therapeutic strategies from an epigenetic perspective.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 3; 455-462
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of the protective efficacy of virus-like particles based on PCV 2b and 2d subtypes against mixed challenge in mice
Autorzy:
Yuan, X.M.
Yuan, Q.C.
Feng, S.M.
Deng, Z.B.
Powiązania:
https://bibliotekanauki.pl/articles/16539142.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
type 2
virus-like particles
vaccine
mouse
Opis:
Porcine circovirus type 2 (PCV2) is an economically important swine pathogen and, although small, it has the highest evolution rate among DNA viruses. Commercial PCV2 commercial vaccines are inactivated PCV2 isolates or a subunit vaccine based on the Cap protein of PCV2. Currently, PCV2 VLPs of individual subtype vaccines are available. Although the main prevalent genotype worldwide is PCV2b, the emerging subtype PCV2d subtype is also increasingly associated with PCV disease. The aim of the study was to evaluate the protective efficacy of VLP based on the PCV2b and 2d subtypes against the mixed challenge of two hypotype PCV2 in mice. Thirty-six female SPV Kunming mice were immunized twice with PCV2b and 2d VLPs, then challenged with PCV2b and PCV2d, to assess the immunogenicity and effectiveness of the VLPs. Vaccination of the mice with PCV2b and 2d VLPs elicited a robust antibody response specific for the PCV2. The virus load detected in the 2b and 2d spleen vaccine group was the lowest compared to other groups. Furthermore, there was no pathological damage in the HE stained sections of the 2b and 2d spleen vaccine, and no virus was detected in the immunohistochemical sections. Our data suggest that the mixed PCV2b and 2d VLP vaccine could protect mice from challenge with the mixed infection of PCV2b and PCV2d.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 2; 195-205
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) in various types of cells in thymic tissues
Autorzy:
Liu, Q.
Yu, Y.-Y.
Wang, H.-Y.
Powiązania:
https://bibliotekanauki.pl/articles/16539177.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
pig
thymus
highly pathogenic porcine reproductive and respiratory syndromevirus
immunofluorescence double labeling method
target cells
Opis:
Thymus, an important central immune organ in pigs, is the site of T lymphocyte development and maturation and an important target organ for infection and replication of various pathogens. Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection results in severe thymic atrophy in piglets. This study aimed to explore the effects of HP-PRRSV on the thymic structure of piglets to elucidate the pathogenesis of thymic atrophy induced by HP-PRRSV. In this study, histopathological techniques and immunofluorescence double staining techniques were used to analyze thymic tissues infected by HP-PRRSV to explore the structural changes of thymus caused by the viral infection and its target cell types. An antibody of cluster of differentiation (CD) 3 (CD3), CD20, CD80, or calgranulin + calprotectin was applied to identify T cells, B cells, dendritic cells (DCs), and macrophages, respectively. The results indicated that a variety of cell components in the thymic tissue were diffusely damaged after viral infection. In the infected thymic tissue, CD80- or calgranulin + calprotectin- -labeled cells supported the HP-PRRSV infection, whereas CD3-labeled T cells and CD20- -labeled B cells did not support the viral infection. The results showed that HP-PRRSV caused the reduction of visible cell components in the thymic tissue, and the virus attacked CD80- and calgranulin + calprotectin-positive cells (such as DCs and macrophages) in the thymic tissue, which played an important role in the pathogenesis of thymus atrophy. These results lay the foundation for elucidating the immunosuppression of piglets after infection with HP-PRRSV.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 2; 287-294
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Female mouse model of diabetes mellitus induced by streptozotocin and high-carbohydrate high-fat diet
Autorzy:
Guo, R.
Dong, J.
Wang, D.Q.
Gu, Y.F.
Powiązania:
https://bibliotekanauki.pl/articles/16627528.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
diabetes mellitus
female mouse
pathological analysis
streptozotocin
high-carbohydrate high-fat diet
Opis:
With the improvement of people’s living standards and rapid economic development, the incidence of diabetes mellitus (DM) is increasing in most parts of the world. DM presents an important potential threat to human health. In the present study, a model of diabetes in female mice was established, and fasting blood glucose was detected at week 4, after which the biochemical profiles were evaluated by histopathological analysis. The success rate of modeling in the normal control (NC) group and the low/ middle/high-dose streptozotocin (STZ) group were 0, 0, 25% and 60%, respectively. In the middle-dose and high-dose STZ groups, the liver index was increased significantly compared with the NC group (p<0.05). The blood biochemical indicators of total cholesterol and low density lipoprotein cholesterol in three STZ injection groups were as follows: alanine aminotransferase and aspartate transaminase in middle- and high-dose STZ groups, high-density lipoprotein cholesterol and serum creatinine in the high-dose STZ group, and blood urea nitrogen in the middle-dose STZ group were significantly increased (p<0.05). The level of total triglycerides was lower, obviously, in the high-dose STZ group than in the NC group (p<0.05). The mice showed marked steatosis, green-dyed fiber tissue coloring in varying degrees, and the contour of the hepatic lobules basically disappeared in STZ injection groups. The results suggest that to establish a diabetes model for female ICR mice, the optimum dose of STZ is 100 mg/kg.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 547-555
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hyperelastic modelling of rubber with multi-walled carbon nanotubes subjected to tensile loading
Autorzy:
Jweeg, M.J.
Alazawi, D.A.
Jebur, Q.H.
Al-Waily, M.
Yasin, N.J.
Powiązania:
https://bibliotekanauki.pl/articles/24200544.pdf
Data publikacji:
2022
Wydawca:
Stowarzyszenie Komputerowej Nauki o Materiałach i Inżynierii Powierzchni w Gliwicach
Tematy:
rubber nanocomposite materials
rubber characterisation
rubber nanocomposite
strain energy
hyperelastic materials model
rubber modelling
gumowe materiały nanokompozytowe
charakterystyka gumy
nanokompozyt gumowy
energia odkształcenia
model materiałów hiperelastycznych
modelowanie gumy
Opis:
Purpose: This study thoroughly examined the application of inverse FE modelling and indentation tensile tests to identify nanotubes' rubber material properties. indentation tensile tests to identify nanotubes' rubber material properties. Design/methodology/approach: Carbon nanotubes with various percentages of multi-walled carbon nanotubes exposed to high tensile stress were used to enhance the mechanical qualities of N.R. rubber. Findings: In this work, carbon nanotubes have been added to natural rubber. By using a solvent casting technique, toluene was used to make nanocomposites. 0.2%, 0.4%, 0.6%, 0.8%, and 1%. In this article, rubber and multi-walled carbon nanotubes interact in practical ways. Mechanical features of carbon nanotubes in NR have been researched. The results will lead to rubber products with improved mechanical qualities compared to present nanocomposite rubber containing various percentages of multi-walled carbon nanotubes exposed to large tensile test loading. The relative fitness error for significant stresses is reasonable with a second or third-order deformation model in numerical results. Research limitations/implications: Non-linear finite element analysis is widely used to optimise complicated elastomeric components' design and reliability studies. However, accurate numerical results cannot be achieved without using rubber or rubber nanocomposite materials with reliable strain energy functions. Practical implications: The indentation tensile tests of rubber samples have been simulated and confirmed using a parametric FE model. An inverse materials parameter identification algorithm was used to calculate the hyperelastic material properties of rubber samples evaluated in uniaxial tensile. Using ABAQUS FE software, material parameters and force-displacement data may be automatically updated and extracted. Originality/value: The numerical data for the inverse method of material property prediction has been successfully established by developing simulation spaces for various material characteristics. The force-displacement curve can be represented using technical methods. The results demonstrate that the inverse FE modelling process might be simplified by using these curve fitting parameters and plot equations to build a mathematical link between curve coefficients and material properties. The first, second, and third-order deformation models were tested using FE simulations for the tensile test.
Źródło:
Archives of Materials Science and Engineering; 2022, 114, 2; 69--85
1897-2764
Pojawia się w:
Archives of Materials Science and Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular dynamics study of the fracture of single layer buckled silicon monosulfide and germanium selenide
Autorzy:
Le, M.-Q
Powiązania:
https://bibliotekanauki.pl/articles/38629974.pdf
Data publikacji:
2022
Wydawca:
Instytut Podstawowych Problemów Techniki PAN
Tematy:
2D materials
fracture
molecular dynamics simulation
mechanical properties
Opis:
Molecular dynamics simulations were conducted with the Stillinger–Weber potential at room temperature to study the mechanical properties and find the mode-I critical stress intensity factor of buckled two-dimensional (2D) hexagonal silicon mono-sulfide (SiS) and germanium selenide (GeSe) sheets. Uniaxial tensile tests were simulated for pristine and pre-cracked sheets. 2D Young’s modulus of SiS and GeSe are estimated at 38.3 and 26.0 N/m, respectively. Their 2D fracture strength is about 3.1–3.5 N/m. By using the initial crack length with the corresponding fracture stress, their mode-I critical stress intensity factor is estimated in the range from 0.19 through 0.22 MPapm. These values differ within 5% from those obtained by the surface energy and are very small compared to the reported fracture toughness of single-crystalline monolayer graphene.
Źródło:
Archives of Mechanics; 2022, 74, 1; 3-12
0373-2029
Pojawia się w:
Archives of Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of friction stir processing parameters of aluminum alloy reinforced with hybrid nanoparticles using the Taguchi method
Autorzy:
Ali, Mohammed Sultan
Al Saffar, Iman Q.
Powiązania:
https://bibliotekanauki.pl/articles/27312410.pdf
Data publikacji:
2022
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
stop aluminium
metoda Taguchi
tarcie
aluminum alloy
friction stir processing
nanoparticles
Taguchi method
ANOVA
Opis:
This study deals with the selection of optimum parameters for friction stir processing of Al alloy 6061-T6 reinforced with a hybrid nanoparticle (B4C and SiO2) in terms of their effect on the mechanical properties (hardness, tensile strength, and wear resistance) using Taguchi method. This work was carried out under four parameters each one running in three levels; rotational speeds (800, 1000 and 1200) rpm, travel speeds (10, 20, and 30) mm/min, holes depth (2, 2.5, and 3) mm, and mixing ratio of (SiO2/B4C) nanoparticles (1/1, 1/2, and 1/3), using L9 (34) Taguchi orthogonal array. Tensile strength and microhardness tests were conducted to evaluate the mechanical properties, in addition to the wear resistance test which is carried out using a pin-on-disk device. The microstructure was examined by optical microscopy, field emission scanning electron microscopy, and x-ray diffraction analysis. It was found that the highest tensile strength (223) MPa at 1200 rpm rotational speed, 30 mm/min traverse speed, 2.5 mm holes depth, and 1/2 (SiO2/B4C) nanoparticles mixing ratio, the highest hardness reached is (155) HV, then decreases in the direction of thermomechanically affected zone (TMAZ), heat affected zone (HAZ), and the base material at (1200) rpm rotational speed, (30) mm/min linear speed, a hole depth of (2) mm and (1/3) mixing ratio of (B4C/SiO2) nanoparticles. The wear behavior was of a mild type or an oxidative type at low loads (5 N), which became severe or metallic wear at higher loads (20 N) at fixed sliding time and speed. The (ANOVA) table has been used to determine which parameter is the most significant using MINITAB software.
Źródło:
International Journal of Applied Mechanics and Engineering; 2022, 27, 4; 13--25
1734-4492
2353-9003
Pojawia się w:
International Journal of Applied Mechanics and Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:
Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:
A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 563

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