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    Wyświetlanie 1-5 z 5
    Tytuł:
    Restriction analysis of genetic variability of Polish isolates of Tomato black ring virus.
    Autorzy:
    Jończyk, Magdalena
    Borodynko, Natasza
    Pospieszny, Henryk
    Powiązania:
    https://bibliotekanauki.pl/articles/1041544.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    restriction analysis
    genetic variability
    phylogeny
    Tomato black ring virus
    Opis:
    Several different isolates of Tomato black ring virus (TBRV) have been collected in Poland from cucumber, tomato, potato and black locust plants. Biological tests showed some differences in the range of infected plants and the type of symptoms, which was the basis for selection of seven the most biologically different TBRV isolates. According to the sequence of TBRV-MJ, several primer pairs were designed and almost the entire sequence of both genomic RNAs was amplified. The RT-PCR products derived from all tested TBRV isolates were digested by restriction enzymes. On the basis of the restriction patterns, the variable and the conserved regions of the TBRV genome were defined and the relationships between the Polish TBRV isolates established.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 3; 673-681
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Complete nucleotide sequence of a Polish strain of Peanut stunt virus (PSV-P) that is related to but not a typical member of subgroup I
    Autorzy:
    Obrepalska-Steplowska, Aleksandra
    Budziszewska, Marta
    Pospieszny, Henryk
    Powiązania:
    https://bibliotekanauki.pl/articles/1040679.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Peanut stunt virus
    satellite RNA
    sequence analysis
    Opis:
    Peanut stunt virus (PSV) is a common legume pathogen present worldwide. It is also infectious for many other plants including peanut and some vegetables. Viruses of this species are classified at present into three subgroups based on their serology and nucleotide homology. Some of them may also carry an additional subviral element - satellite RNA. Analysis of the full genome sequence of a Polish strain - PSV-P - associated with satRNA was performed and showed that it may be classified as a derivative of the subgroup I sharing 83.9-87.9% nucleotide homology with other members of this subgroup. A comparative study of sequenced PSV strains indicates that PSV-P shows the highest identity level with PSV-ER or PSV-J depending on the region used for analysis. Phylogenetic analyses, on the other hand, have revealed that PSV-P is related to representatives of the subgroup I to the same degree, with the exception of the coat protein coding sequence where PSV-P is clustered together with PSV-ER.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 4; 731-739
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Full length genome sequence of Polish isolate of Beet soil-borne virus confirms low level of genetic diversity
    Autorzy:
    Borodynko, Natasza
    Hasiów-Jaroszewska, Beata
    Rymelska, Natalia
    Pospieszny, Henryk
    Powiązania:
    https://bibliotekanauki.pl/articles/1040498.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    BSBV
    genetic diversity
    Polymyxa betae
    Opis:
    The complete nucleotide sequence of a Polish isolate of Beet soil-borne virus was determined for the first time. The genome organization was identical with those previously established for isolates from Germany and China. A comparison of the Polish isolate with others deposited in GenBank reveled high level of nucleotide identity, about 98-100%, throughout the genome analyzed. The ratio between non-synonymous and synonymous substitutions was rather low suggesting a negative selective pressure. The non-synonymous mutations were particulary frequent in triple gene block.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 4; 729-731
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evidence for RNA recombination between distinct isolates of Pepino mosaic virus
    Autorzy:
    Hasiów-Jaroszewska, Beata
    Kuzniar, Arnold
    Peters, Sander
    Leunissen, Jack
    Pospieszny, Henryk
    Powiązania:
    https://bibliotekanauki.pl/articles/1040390.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    genetic diversity
    recombination
    PepMV
    Opis:
    Genetic recombination plays an important role in the evolution of virus genomes. In this study we analyzed publicly available genomic sequences of Pepino mosaic virus (PepMV) for recombination events using several bioinformatics tools. The genome-wide analyses not only confirm the presence of previously found recombination events in PepMV but also provide the first evidence for double recombinant origin of the US2 isolate.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 3; 385-388
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biopolymer compositions for ecological protection and growth stimulation of plants
    Autorzy:
    Niekraszewicz, Antoni
    Wiśniewska-Wrona, Maria
    Kopania, Ewa
    Orlikowski, Leszek
    Pospieszny, Henryk
    Krawczyk, Krzysztof
    Powiązania:
    https://bibliotekanauki.pl/articles/1035390.pdf
    Data publikacji:
    2012
    Wydawca:
    Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
    Tematy:
    biopolymer compositions
    growth stimulation.
    plant protection
    polysaccharides
    Opis:
    Presented herein are investigations in the preparation of an advanced , human-and environmentfriendly plant protection composition based on selected bioactive biopolymers of the polysaccharide family. In the Institute of Biopolymers and Chemical Fibres the biopolymers were prepared and the biological activity of chitosan in the form of salt and gel and of hemicelluloses (galactoglucomannans) and of their compositions was tentatively evaluated by way of plate tests. Estimated was the impact of such preparations upon the stimulation of the germination rate of radish seeds at concentration of 0.1, 0.01 and 0.005% after 72 hours. The efficacy of the preparations was evaluated based on the number of germinated seeds in the green mass of the sprouts and their length in comparison to a reference in water at pH = 7.0. In Research Institute of Horticulture, Skierniewice, Poland the usefulness of selected iopolymer preparations was evaluated as agents to protect decorative plants against some pathogens (on leaves, in soil) such as Phytophtora cryptogea causing decay of shoots and roots. It could be found that the biopolymer compositions applied to the soil provided a much better rooting of chrysanthemum cuttings in a Phytophtora cryptogea - infected soil. The cuttings were growing much faster and the number of infected ones was largely reduced. The usefulness of a number of chitosan formulations was also evaluated for seed pickling. It was found that the formulations exerted a distinctly positive impact upon the germination, growth and health of the seedlings. Laboratory and greenhouse testing was made in the Institute of Plant Protection, Poznań, Poland where the biological activity of some biopolymer compositions was evaluated. A first selection of the biopolymers was done based on an in vitro examination of the impact upon bacteria growth. (Gram negative - Erwinia amylovora and Gram positive Clavibacter ichiganensis ) on agar or agarose plates and upon the generation of necrotic stains on beans and tobacco caused by lucerne mosaic virus and tobacco mosaic virus respectively. It was found that the preparations with original biopolymer concentration had not affected the viruses directly but stimulated the plants’ immunity against the pathogens.
    Źródło:
    Progress on Chemistry and Application of Chitin and its Derivatives; 2012, 17; 149-162
    1896-5644
    Pojawia się w:
    Progress on Chemistry and Application of Chitin and its Derivatives
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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