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    Wyświetlanie 1-3 z 3
    Tytuł:
    Evaluation of the effects of antibiotics on cytotoxicity of EGFP and DsRed2 fluorescent proteins used for stable cell labeling
    Autorzy:
    Zakrzewska, Karolina
    Samluk, Anna
    Pluta, Krzysztof
    Pijanowska, Dorota
    Powiązania:
    https://bibliotekanauki.pl/articles/1039220.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    stable fluorescent labeling
    whole-cell labeling
    fluorescent protein cytotoxicity
    Opis:
    The use of fluorescent markers has proven to be an attractive tool in biological imaging. However, its usefulness may be confined by the cytotoxicity of the fluorescent proteins. In this article, for the first time, we have examined an influence of the antibiotics present in culture medium on cytotoxicity of the EGFP and DsRed2 markers used for whole-cell labeling. Results showed that doxycycline negatively affected albumin synthesis in DsRed2-expressing hepatoma cells, and that both hepatoma cells and human skin fibroblasts, labeled with this protein, were characterized by the lowered growth rates. Thus, the cytotoxic effect of fluorescent markers depends on both protein used for cell labeling and on growth conditions that may cause cell stress.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 4; 809-813
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of geometry and annealing temperature in argon atmosphere of TiO2 nanotubes on their electrochemical properties
    Autorzy:
    Nycz, Marta
    Paradowska, Ewa
    Arkusz, Katarzyna
    Pijanowska, Dorota Genowefa
    Powiązania:
    https://bibliotekanauki.pl/articles/307103.pdf
    Data publikacji:
    2020
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    dwutlenek tytanu
    TiO2
    modyfikacja termiczna
    wyżarzanie
    titanium dioxide (TiO2)
    titanium nanotubes
    thermal modification
    annealing
    Opis:
    In this paper, electrochemical properties of the as-formed and thermally treated titanium dioxide (TiO2) nanotubes with diameter in the range of 20–100 nm and height in the range of 100–1000 nm were presented. In addition, the effects of annealing temperature (450–550 °C) on the electrochemical characteristics of these structures, as well as the influence of diameter and height of TiO2 nanotubes on these properties were examined. The results were referred to a compact TiO2 layer (100 nm thick). Methods: The electrochemical test included open circuit potential, impedance spectroscopy and cyclic voltammetry measurements. The scanning electron microscope with energy dispersive spectroscopy analyser, x-ray photoelectron spectroscopy, and x-ray diffraction analysers were used for surface morphology characterisation as well as elemental, phase and chemical composition of TiO2 layers. Results: It was found that nanotubes with the diameter of 50 and 75 nm (height of 1000 nm) annealed at 550 °C exhibit the lowest impedance and phase angle values. However, the voltammetric detection of potassium ferricyanide indicated that the closest to 1 Ipc /Ipa ratio were shown by nanotubes with a diameter of 50 and 75 nm annealed at 450 °C. Conclusions: On the basis of performed analysis, it can be stated that the TiO2 layer with nanotubes of 50 nm in diameter and of 1000 nm in height, annealed in 450 °C may be indicated as the ones having the most favourable sensing and biosensing properties.
    Źródło:
    Acta of Bioengineering and Biomechanics; 2020, 22, 1; 165-177
    1509-409X
    2450-6303
    Pojawia się w:
    Acta of Bioengineering and Biomechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dried human skin fibroblasts as a new substratum for functional culture of hepatic cells
    Autorzy:
    Wencel, Agnieszka
    Zakrzewska, Karolina
    Samluk, Anna
    Noszczyk, Bartłomiej
    Pijanowska, Dorota
    Pluta, Krzysztof
    Powiązania:
    https://bibliotekanauki.pl/articles/1038663.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    cocultures
    culture substratum
    dried fibroblasts
    human skin fibroblasts
    C3A cells
    Opis:
    The primary hepatocytes culture is still one of the main challenges in toxicology studies in the drug discovery process, development of in vitro models to study liver function, and cell-based therapies. Isolated hepatocytes display a rapid decline in viability and liver-specific functions including albumin production, conversion of ammonia to urea, and activity of the drug metabolizing enzymes. A number of methods have been developed in order to maintain hepatocytes in their highly differentiated state in vitro. Optimization of culture conditions includes a variety of media formulations and supplements, growth surface coating with the components of extracellular matrix or with synthetic polymers, three-dimensional growth scaffolds and decellularized tissues, and coculture with other cell types required for the normal cell-cell interactions. Here we propose a new substratum for hepatic cells made by drying confluent human skin fibroblasts' culture. This growth surface coating, prepared using maximally simplified procedure, combines the advantages of the use of extracellular matrices and growth factors/cytokines secreted by the feeder layer cells. In comparison to the hepatoma cells grown on a regular tissue culture plastic, cells cultured on the dried fibroblasts were able to synthesize albumin in larger quantities and to form greater number of apical vacuoles. Unlike the coculture with the living feeder layer cells, the number of cells grown on the new substratum was not reduced after fourteen days of culture. This fact could make the dried fibroblasts coating an ideal candidate for the substrate for non-dividing human hepatocytes.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 2; 357-363
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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