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Wyświetlanie 1-4 z 4
Tytuł:
Hydrogel scaffolds for medical applications obtained by indirect 3D-printing method
Autorzy:
Pietryga, Krzysztof
Pamuła, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/284894.pdf
Data publikacji:
2019
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Źródło:
Engineering of Biomaterials; 2019, 22, no.153; 93
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Composite scaffolds enriched with calcium carbonate microparticles loaded with epigallocatechin gallate for bone tissue regeneration
Autorzy:
Pietryga, Krzysztof
Panaite, Anca-Alexandra
Pamuła, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/24200847.pdf
Data publikacji:
2022
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
gellan gum
gelatin
hydrogel
calcium carbonate microparticles
enzymatic mineralization
epigallocatechin gallate (EGCG)
Opis:
There is a need to develop advanced multifunctional scaffolds for the treatment of bone tissue lesions, which apart from providing support for infiltrating cells could assure the delivery of drugs or biologically active molecules enhancing bone formation. We developed composite scaffolds for bone tissue engineering based on gellan gum (GG) and gelatin (Gel) hydrogel enriched with epigallocatechin gallate (EGCG) loaded CaCO3 microparticles and subjected to enzymatic mineralization with calcium phosphate (CaP). The method of manufacturing CaCO3 microparticles was optimized. The EGCG-loaded microparticles were smaller than those unloaded, and the release of EGCG was prolonged for up to 14 days, as shown by the Folin-Ciocalteu test. The particles reduced the viability of the MG-63 cells as compared to the control. However, when they were loaded with EGCG, their cytotoxicity was reduced. The particles were suspended in a GG/Gel hydrogel containing alkaline phosphatase (ALP), soaked in calcium glycerophosphate (CaGP) solution to create CaP deposits, and submitted to freeze-drying, in order to produce a porous scaffold. The microstructure of the scaffolds was characterized by optical and scanning electron microscopy and showed that the size of the pores corresponds to that of the spongy bone. In vitro tests with MG-63 cells confirmed that mineralized scaffolds support cell adhesion and growth to a higher extent than nonmineralized ones.
Źródło:
Engineering of Biomaterials; 2022, 25, 166; 12--21
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Elastic, electrically conductive, cytocompatible and bioprintable composites
Autorzy:
Benko, Aleksandra
Wilk, Sabastian
Martyniak, Alicja
Stępniewski, Jacek
Zambrzycki, Marcel
Pietryga, Krzysztof
Stedncel, Marek
Dulak, Józef
Powiązania:
https://bibliotekanauki.pl/articles/1844753.pdf
Data publikacji:
2021
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Źródło:
Engineering of Biomaterials; 2021, 24, 163; 108
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phenolic plant extract enrichment of enzymatically mineralized hydrogels
Autorzy:
Douglas, Timothy E.L
Lopez-Heredia, Marco A.
Pułczyńska, Aleksandra
Łapa, Agata
Pietryga, Krzysztof
Schaubroeck, David
Santos, Sónia A.O.
Pais, Adriana
Brackman, Gilles
Schamphelaere, Karel de
Samal, Sangram Keshari
Keppler, Julia K.
Bauer, Jonas L
Chai, Feng
Blanchemain, Nicolas
Coenye, Tom
Pamuła, Elżbieta
Skirtach, Andre G
Powiązania:
https://bibliotekanauki.pl/articles/284057.pdf
Data publikacji:
2019
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
hydrogel
mineralization
polyphenols
antibacterial
epigallocatechin gallate
gellan gum
Opis:
Hydrogel mineralization with calcium phosphate (CaP) and antibacterial activity are desirable for applications in bone regeneration. Mineralization with CaP can be induced using the enzyme alkaline phosphatase (ALP), responsible for CaP formation in bone tissue. Incorporation of polyphenols, plant-derived bactericidal molecules, was hypothesized to provide antibacterial activity and enhance ALP-induced mineralization. Three phenolic rich plant extracts from: (i) green tea, rich in epigallocatechin gallate (EGCG) (herafter referred to as EGCG-rich extract); (ii) pine bark and (iii) rosemary were added to gellan gum (GG) hydrogels and subsequently mineralized using ALP. The phenolic composition of the three extracts used were analyzed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MSn). EGCG-rich extract showed the highest content of phenolic compounds and promoted the highest CaP formation as corroborated by dry mass percentage meassurements and ICP-OES de-termination of mass of elemental Ca and P. All three extracts alone exhibited antibacterial activity in the following order EGCG-rich > PI > RO, respectively. However, extract-loaded and mineralized GG hydro-gels did not exhibit appreciable antibacterial activity by diffusion test. In conclusion, only the EGCG-rich extract promotes ALP-mediated mineralization.
Źródło:
Engineering of Biomaterials; 2019, 22, 149; 2-9
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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