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    Wyświetlanie 1-5 z 5
    Tytuł:
    In vitro regeneration of Centaurium erythraea Rafn from shoot tips and other seedling explants
    Autorzy:
    Piatczak, E
    Wysokinska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/56437.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Centaurium erythraea
    micropropagation
    seedling explant
    shoot tip
    regeneration
    organogenesis
    in vitro
    Gentianaceae
    Opis:
    Various explants from 30-day-old seedlings of Centaurium erythraea Rafn were evaluated for their morphogenetic capacity under in vitro culture conditions. Shoot formation from shoot tip explants was achieved mainly through adventitious bud differentiation. The highest number of shoots (up to 43.3 ± 2.2 from a single shoot tip) was obtained on Murashige and Skoog medium (MS) supplemented with indole-3-acetic acid (IAA) (0.57 μM) and 6-benzylaminopurine (BAP) (4.4 μM). Adventitious shoot regeneration was also achieved through organogenesis from calluses obtained from hypocotyls, cotyledons, roots and leaves on MS medium containing IAA (2.85 μM) and BAP (0.88 μM). Significant differences were noted between explant types in their effects on shoot regeneration. In the primary culture, the best response was obtained either from calluses derived from roots or leaves (44.4 ± 4.5 and 40.2 ± 6.0 shoots per callus, respectively). The number of subcultures of inoculated calluses affected both the multiplication rate (the number of shoots/explant) and shoot morphology (the frequency of shoot hyperhydricity). Shoots rooted with the frequency of 94-100% after culture on MS medium without growth regulators. Plantlets were successfully acclimatized (97%) under high relative humidity and then moved to the greenhouse.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2003, 72, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Encapsulation of Centaurium Erythraea Rafn – an Efficient Method for Regeneration of Transgenic Plants
    Autorzy:
    Piatczak, E.
    Wysokinska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/19616.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2013, 55, 2
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Regeneracja pędów przybyszowych w kulturze in vitro Centaurium eryhraea RAFN.
    Adventitious shoot regeneration in in vitro culture of Centaurium erythraea RAFN.
    Autorzy:
    Piatczak, E.
    Wysokinska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/802783.pdf
    Data publikacji:
    2002
    Wydawca:
    Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
    Źródło:
    Zeszyty Problemowe Postępów Nauk Rolniczych; 2002, 488, 2
    0084-5477
    Pojawia się w:
    Zeszyty Problemowe Postępów Nauk Rolniczych
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Morphology, secoiridoid content and RAPD analysis of plants regenerated from callus of Centaurium erythraea Rafn.
    Autorzy:
    Piatczak, E.
    Krolicka, A.
    Wysokinska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/19786.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Centaurium erythraea plants obtained by indirect organogenesis are described in the paper. The plants were initiated from a single adventitious shoot regenerated from callus derived from the cotyledon of a 30-day-old seedling. The shoot was multiplied on MS medium supplemented with IAA (0.1 mg·L-1) and BAP (1.0 mg·L-1). The multiplication rate (28 shoots per culture within 4 weeks) was highest at the first subculture and decreased in further subcultures. The shoots were rooted on MS medium. The effect of IBA (0.1 mg·L-1) on the number of shoots forming roots differed depending on the composition of the basal medium (MS). The rooted shoots were transplanted to soil and grown in a greenhouse with 90% effectiveness. RAPD analysis was done with adventitious shoots of C. erythraea from in vitro culture. In shoots and whole plants regenerated from the callus tissue, secoiridoid content was determined by the HPLC method. We showed significant differences in morphology (leaf size, fresh and dry weight and height of plants) and changes in the DNA profiles as compared to earlier reports for shoot tip-derived shoots and plants of C. erythraea, but the two groups of plants biosynthesized the same qualitative pattern and similar levels of secoiridoids, up to 150 mg·g-1 dry weight; the increased biomass of plants regenerated from callus tissue makes them a better source of secondary metabolites.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 2
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of methyl jasmonate and salicylic acid on secondary metabolite production in Rehmannia glutinosa Libosch. Hairy root culture
    Autorzy:
    Piatczak, E.
    Kuzma, L.
    Wysokinska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/19795.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Rehmannia glutinosa hairy roots were used to evaluate the effect of methyl jasmonate (MeJa) and salicylic acid (SA) on increase of root biomass and production of iridoids (catalpol, harpagide) and phenylethanoids (verbascoside and isoverbascoside). The elicitors were added to 23-day-old culture separately at concentrations between 50 and 200 μM or in combinations at concentrations of 50 and 100 μM. Roots were harvested 72 h and 120 h after elicitation. The type of elicitor, its concentration and exposure time were found to strongly affect the content of each analyzed compound. A 72-hour treatment with 200 μM MeJa was the most effective in increase of verbascoside content (60.07 mg·DWˉ¹ equivalent to 845.45 mg·Lˉ¹) and isoverbascoside (1.77 mg·DWˉ¹ equivalent to 24.94 mg·Lˉ¹): these respective amounts were roughly 10- and 6.4-fold higher than the control values (unelicited roots). Exposure to 150 μM MeJa provided optimal harpagide content after 72 hours (0.136 mg·DWˉ¹; 7.5-fold increase compared to the control), and catalpol content after 120 hours (up to 2.145 mg·DWˉ¹). The combination of MeJa and SA also resulted in higher levels of secondary metabolites compared to the control culture, although these levels were lower than those observed for MeJa alone at the optimal concentration and exposure time. SA alone was less efficient in enhancing metabolite production than MeJa.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2016, 58, 1
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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