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    Wyświetlanie 1-3 z 3
    Tytuł:
    The future of infrared spectroscopy in biosciences: In vitro, time-resolved, and 3D
    Autorzy:
    Chen, Hsiang-Hsin
    Bobroff, V.
    Delugin, M.
    Pineau, R.
    Noreen, Razia
    Seydou, Yao
    Banerjee, S.
    Chatterjee, J.
    Javerzat, S.
    Petibois, C.
    Powiązania:
    https://bibliotekanauki.pl/articles/1065164.pdf
    Data publikacji:
    2016-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    87.10.Ca
    Opis:
    Infrared (IR) spectroscopy is at the cross-roads, with the requirement to compete with cutting-edge technologies in biosciences, mostly based on analytical performances dealing with the super-resolutions: time, lateral/spatial, and contrast. IR microscopy is diffraction limited in most cases, thus not accessing to high lateral/spatial resolutions. Additionally, it has a poor signal-to-noise ratio on a single scan, thus requiring long-lasting acquisitions that are not suitable to analyze ns-lasting biochemical events. However, it is unique because it provides a broad global chemical information of the sample contents. It is also unique because it does not require heavy sample preparation nor labeling and can be coupled to other techniques (multimodality). Finally, it is again unique because it provides quantitative measurements, thus suitable for 1D to 4D data exploitation procedures. This short review shows that IR spectroscopy will be certainly subjected to a second century of innovations, maintaining its influence in the panorama of cutting-edge analytical techniques.
    Źródło:
    Acta Physica Polonica A; 2016, 129, 2; 255-259
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Methodology for FTIR Imaging of Individual Cells
    Autorzy:
    Yao, Seydou
    Cestelli Guidi, M.
    Delugin, M.
    Della-Ventura, G.
    Marcelli, A.
    Petibois, C.
    Powiązania:
    https://bibliotekanauki.pl/articles/1398831.pdf
    Data publikacji:
    2016-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    82.80.-d
    87.85.Pq
    87.64.-t
    Opis:
    FTIR imaging is a novel spectroscopic technique able to provide cell imaging, in vivo and in real-time. However, one key issue is developing methodologies for cell culture on IR-transparent substrates fitting cell biology requirements. In this work we tested different IR-transparent substrates in terms of biotoxicity, surface properties, and spectral image acquisition qualities. Only a few substrates, namely Si₃N₄, Ge, GLS, LaF₃, Si, SrF₂, ZnS/C, ZnS/F, were found to provide cell culture conditions comparable to those observed on usual polycarbonate Petri dishes, the main limiting parameter being the toxicity of the material (ZnS, GLS, PbF₂, PbCl₂) or a poor adhesiveness (notably diamond, AgCl, CaF₂, ZnS). From substrates eligible for a good-quality cell culture, the spectral acquisition quality is mainly affected by the refractive index value. Finally, the best compromise between cell culture quality and image spectral quality could be obtained using Si and Ge substrates. This rationalization of the available IR-transparent substrates for bioimaging is particularly relevant for live cell analyses, where cell culture conditions must remain unaffected by substrate properties.
    Źródło:
    Acta Physica Polonica A; 2016, 129, 2; 250-254
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Current Trends in the Development of FTIR Imaging for the Quantitative Analysis of Biological Samples
    Autorzy:
    Petibois, C.
    Wehbe, K.
    Belbachir, K.
    Noreen, R.
    Déléris, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/1808575.pdf
    Data publikacji:
    2009-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    82.80.-d
    Opis:
    Fourier transform infrared imaging instrumentation has come of age for the rapid acquisition of biosample IR images, and thus now allows developing analytical methods based upon the molecular information of samples contents. For the biomedicine field, Fourier transform infrared imaging should be able to play a role in the molecular characterization of cells and tissues where no other analytical method provides quantitative information. Now, a compromise may be obtained between an acceptable acquisition time of IR images, which should never exceed a few tens of minutes, and the necessary spatial resolution (down to the diffraction limit, although limited for biology), spectral resolution (2 to 8 $cm^{-1}$ for biosample analyses), and signal-to-noise ratio level, to provide a diagnostic answer to clinicians during the surgery time. Here, we will discuss the potential of Fourier transform infrared imaging in face to major pathologies (myopathies, brain tumors, metabolic diseases) for which current imaging methods remain unable to provide sufficient information for a precise diagnosis. Quantitative molecular information may be extracted from infrared images of samples as soon as samples volume/thickness is controlled as well as absorption and absorptivity of the molecules to analyze may be isolated from other absorbing compounds. In this context, metabolic parameters appear as the main targets for providing critical information about the physiological status of a biosample due to their characteristic infrared spectra. As examples, it will be shown how to isolate and quantify glucose, lactic-acid, urea, etc. absorptions from complex biosample infrared images.
    Źródło:
    Acta Physica Polonica A; 2009, 115, 2; 507-512
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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