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Wyświetlanie 1-7 z 7
Tytuł:
Grazing of Native Livestock Breeds as a Method of Grassland Protection in Roztocze National Park, Eastern Poland
Autorzy:
Kulik, Mariusz
Warda, Marianna
Gawryluk, Adam
Bochniak, Andrzej
Patkowski, Krzysztof
Lipiec, Antoni
Gruszecki, Tomasz
Pluta, Michał
Bielińska, Elżbieta
Futa, Barbara
Powiązania:
https://bibliotekanauki.pl/articles/123977.pdf
Data publikacji:
2020
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
animal welfare
grasses
herb
weed
horse
sheep
Opis:
The studies were conducted in a forest settlement in Roztocze National Park (eastern Poland). The aim of the study was to evaluate the vegetation of two pastures depending on the type of use in the context of grassland protection, identify the trends of species composition changes, and analyse the yield and nutritional value of the biomass in the context of animal welfare. The studies were conducted on permanent grasslands varying in terms of fertility and location. Both sites were pastures where native breeds of livestock (Polish Lowland sheep of the Uhrusk and Polish Konik) were grazing. The fertile pasture was represented by the developing Lolio-Cynosuretum association, while the poor dry pasture – by a community with Common Bent (Agrostis capillaris L.) and a community with Mouse-Ear Hawkweed (Hieracium pilosella L.). The greatest changes over time were observed in the mowed site in the fertile pasture (increased share of tall grasses) and in the abandoned poor dry pasture (increased share of herbs and weeds). Livestock grazing conducted from 2010 influenced the stabilisation of the species composition. Tree and shrub seedlings were systematically eaten by livestock, which evidences a positive impact of grazing on the preservation of permanent grasslands in Roztocze National Park where forest ecosystems predominate. The assessment of the species composition and yielding indicated that the fertile pasture was characterised by good value while the poor pasture – low or sufficient value. In terms of nutrient yield and content, these pastures were poor or very poor, and their nutritive potential was largely dependent on the meteorological conditions. While the livestock density in the pastures, ranging from 1.1 to 1.5 LSU ha–1 in the years under study, was appropriate, grazing should be limited in the summer months, particularly in periods of drought, by reducing the number of animals or by additional feeding to ensure their welfare.
Źródło:
Journal of Ecological Engineering; 2020, 21, 3; 61-69
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cytotoxicity of thermo-responsive polymeric nanoparticles based on N-isopropylacrylamide for potential application as a bioscaffold
Autorzy:
Deptuła, Tobiasz
Warowicka, Alicja
Woźniak, Anna
Grzeszkowiak, Mikołaj
Jarzębski, Maciej
Bednarowicz, Magdalena
Patkowski, Adam
Słomski, Ryszard
Powiązania:
https://bibliotekanauki.pl/articles/1039109.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
nanoparticles
toxicology
poly-N-isopropylacrylamide
drug delivery system
cytotoxicity
polymers
Opis:
Polymeric nanoparticles based on poly-N-isopropylacrylamide (pNiPAM NPs) and their bio-medical applications have been widely investigated in recent years. These tunable nanoparticles are considered to be great candidates for drug delivery systems, biosensors and bioanalytical devices. Thus, the biocompatibility and toxicity of these nanoparticles is clearly a crucial issue. In this work, the cytotoxicity of thermo-responsive pNiPAM nanoparticles was studied, followed by a detailed analysis of the NPs morphology in growing cell cultures and their 3D structure. Cytotoxic examination was conducted for two cell cultures - HeLa (cervical cancer cell line) and HeK293 (human embryonic kidney cell line), employing MTT (3-4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide) assay and viability tests. We used Cryo-SEM (scanning electron microscopy) and fluorescence microscopy (IN Cell Analyzer) in order to investigate the morphological structure of the polymer network. We show that pNiPAM nanoparticles do not exhibit any cytotoxicity effects on the investigated cell lines. Additionally, we report that the pNiPAM nanoparticle based scaffold promotes cell growth.
Źródło:
Acta Biochimica Polonica; 2015, 62, 2; 311-316
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of mono- and divalent cations on Tetrahymena thermophila telomeric repeat fragment. A photon correlation spectroscopy study.
Autorzy:
Włodarczyk, Agnieszka
Patkowski, Adam
Grzybowski, Przemysław
Dobek, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1041510.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
chromosome ends
G-quadruplex
telomeric DNA
photon correlation spectroscopy
structure transition
Opis:
The structure of the Tetrahymena thermophila telomeric sequence d(TGGGGT)4 was studied by photon correlation spectroscopy (PCS) in aqueous solution in the presence of NaCl, KCl and SrCl2. The sample studied was polydisperse in all conditions studied. Translational diffusion coefficients DT describing the diffusion modes observed were determined. On the basis of a comparison between the experimental DT values with those calculated assuming the bead model, two forms were identified as telomeric quadruplex structures: monomer and tetramer. In the presence of SrCl2 formation of aggregates was observed, with a size that reached several micrometres. The relative weighted concentrations of the structures observed for different concentrations of a salt and DNA were determined. The results obtained in the presence of monovalent ions were qualitatively similar and could be presented in a coherent plot in which the concentration of salt was expressed by the number of ions per DNA molecule. A large number of ions per DNA molecule favoured tetramer formation while a small number favoured the monomer form. A structural phase transition from the monomer to the tetramer induced by a change in the number of ions per DNA molecule was observed. The main difference between the results for Na+ and K+ was a greater effectiveness of the K+ ions in formation of tetramers. The effect of Sr2+ ions on the structures formed was different than that of the monovalent ions. The results obtained in the presence of Sr2+ could not be described as a function of the number of ions per DNA molecule.
Źródło:
Acta Biochimica Polonica; 2004, 51, 4; 971-981
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of sodium ion concentration on transfer RNA conformation in solution studied by Rayleigh light scattering
Autorzy:
Patkowski, Adam
Dobek, Andrzej
Labuda, Damian
Powiązania:
https://bibliotekanauki.pl/articles/1046134.pdf
Data publikacji:
1981
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1981, 28, 3-4; 337-349
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-7 z 7

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