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    Wyświetlanie 1-7 z 7
    Tytuł:
    Effect of sodium ion concentration on transfer RNA conformation in solution studied by Rayleigh light scattering
    Autorzy:
    Patkowski, Adam
    Dobek, Andrzej
    Labuda, Damian
    Powiązania:
    https://bibliotekanauki.pl/articles/1046134.pdf
    Data publikacji:
    1981
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1981, 28, 3-4; 337-349
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of mono- and divalent cations on Tetrahymena thermophila telomeric repeat fragment. A photon correlation spectroscopy study.
    Autorzy:
    Włodarczyk, Agnieszka
    Patkowski, Adam
    Grzybowski, Przemysław
    Dobek, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1041510.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    chromosome ends
    G-quadruplex
    telomeric DNA
    photon correlation spectroscopy
    structure transition
    Opis:
    The structure of the Tetrahymena thermophila telomeric sequence d(TGGGGT)4 was studied by photon correlation spectroscopy (PCS) in aqueous solution in the presence of NaCl, KCl and SrCl2. The sample studied was polydisperse in all conditions studied. Translational diffusion coefficients DT describing the diffusion modes observed were determined. On the basis of a comparison between the experimental DT values with those calculated assuming the bead model, two forms were identified as telomeric quadruplex structures: monomer and tetramer. In the presence of SrCl2 formation of aggregates was observed, with a size that reached several micrometres. The relative weighted concentrations of the structures observed for different concentrations of a salt and DNA were determined. The results obtained in the presence of monovalent ions were qualitatively similar and could be presented in a coherent plot in which the concentration of salt was expressed by the number of ions per DNA molecule. A large number of ions per DNA molecule favoured tetramer formation while a small number favoured the monomer form. A structural phase transition from the monomer to the tetramer induced by a change in the number of ions per DNA molecule was observed. The main difference between the results for Na+ and K+ was a greater effectiveness of the K+ ions in formation of tetramers. The effect of Sr2+ ions on the structures formed was different than that of the monovalent ions. The results obtained in the presence of Sr2+ could not be described as a function of the number of ions per DNA molecule.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 4; 971-981
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cytotoxicity of thermo-responsive polymeric nanoparticles based on N-isopropylacrylamide for potential application as a bioscaffold
    Autorzy:
    Deptuła, Tobiasz
    Warowicka, Alicja
    Woźniak, Anna
    Grzeszkowiak, Mikołaj
    Jarzębski, Maciej
    Bednarowicz, Magdalena
    Patkowski, Adam
    Słomski, Ryszard
    Powiązania:
    https://bibliotekanauki.pl/articles/1039109.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    nanoparticles
    toxicology
    poly-N-isopropylacrylamide
    drug delivery system
    cytotoxicity
    polymers
    Opis:
    Polymeric nanoparticles based on poly-N-isopropylacrylamide (pNiPAM NPs) and their bio-medical applications have been widely investigated in recent years. These tunable nanoparticles are considered to be great candidates for drug delivery systems, biosensors and bioanalytical devices. Thus, the biocompatibility and toxicity of these nanoparticles is clearly a crucial issue. In this work, the cytotoxicity of thermo-responsive pNiPAM nanoparticles was studied, followed by a detailed analysis of the NPs morphology in growing cell cultures and their 3D structure. Cytotoxic examination was conducted for two cell cultures - HeLa (cervical cancer cell line) and HeK293 (human embryonic kidney cell line), employing MTT (3-4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide) assay and viability tests. We used Cryo-SEM (scanning electron microscopy) and fluorescence microscopy (IN Cell Analyzer) in order to investigate the morphological structure of the polymer network. We show that pNiPAM nanoparticles do not exhibit any cytotoxicity effects on the investigated cell lines. Additionally, we report that the pNiPAM nanoparticle based scaffold promotes cell growth.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 2; 311-316
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Grazing of Native Livestock Breeds as a Method of Grassland Protection in Roztocze National Park, Eastern Poland
    Autorzy:
    Kulik, Mariusz
    Warda, Marianna
    Gawryluk, Adam
    Bochniak, Andrzej
    Patkowski, Krzysztof
    Lipiec, Antoni
    Gruszecki, Tomasz
    Pluta, Michał
    Bielińska, Elżbieta
    Futa, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/123977.pdf
    Data publikacji:
    2020
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    animal welfare
    grasses
    herb
    weed
    horse
    sheep
    Opis:
    The studies were conducted in a forest settlement in Roztocze National Park (eastern Poland). The aim of the study was to evaluate the vegetation of two pastures depending on the type of use in the context of grassland protection, identify the trends of species composition changes, and analyse the yield and nutritional value of the biomass in the context of animal welfare. The studies were conducted on permanent grasslands varying in terms of fertility and location. Both sites were pastures where native breeds of livestock (Polish Lowland sheep of the Uhrusk and Polish Konik) were grazing. The fertile pasture was represented by the developing Lolio-Cynosuretum association, while the poor dry pasture – by a community with Common Bent (Agrostis capillaris L.) and a community with Mouse-Ear Hawkweed (Hieracium pilosella L.). The greatest changes over time were observed in the mowed site in the fertile pasture (increased share of tall grasses) and in the abandoned poor dry pasture (increased share of herbs and weeds). Livestock grazing conducted from 2010 influenced the stabilisation of the species composition. Tree and shrub seedlings were systematically eaten by livestock, which evidences a positive impact of grazing on the preservation of permanent grasslands in Roztocze National Park where forest ecosystems predominate. The assessment of the species composition and yielding indicated that the fertile pasture was characterised by good value while the poor pasture – low or sufficient value. In terms of nutrient yield and content, these pastures were poor or very poor, and their nutritive potential was largely dependent on the meteorological conditions. While the livestock density in the pastures, ranging from 1.1 to 1.5 LSU ha–1 in the years under study, was appropriate, grazing should be limited in the summer months, particularly in periods of drought, by reducing the number of animals or by additional feeding to ensure their welfare.
    Źródło:
    Journal of Ecological Engineering; 2020, 21, 3; 61-69
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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