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Wyświetlanie 1-15 z 20
Tytuł:
Bioactive ceramic coating formed on Ti bone wedge
Autorzy:
Kazek-Kęsik, Alicja
Krok-Borkowicz, Małgorzata
Pamuła, Elżbieta
Simka, Wojciech
Powiązania:
https://bibliotekanauki.pl/articles/1844942.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
ceramics
bioactivity
implants
ceramika
bioaktywność
implanty
Źródło:
Engineering of Biomaterials; 2020, 23, 158 spec. iss.; 50
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Composite scaffolds enriched with calcium carbonate microparticles loaded with epigallocatechin gallate for bone tissue regeneration
Autorzy:
Pietryga, Krzysztof
Panaite, Anca-Alexandra
Pamuła, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/24200847.pdf
Data publikacji:
2022
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
gellan gum
gelatin
hydrogel
calcium carbonate microparticles
enzymatic mineralization
epigallocatechin gallate (EGCG)
Opis:
There is a need to develop advanced multifunctional scaffolds for the treatment of bone tissue lesions, which apart from providing support for infiltrating cells could assure the delivery of drugs or biologically active molecules enhancing bone formation. We developed composite scaffolds for bone tissue engineering based on gellan gum (GG) and gelatin (Gel) hydrogel enriched with epigallocatechin gallate (EGCG) loaded CaCO3 microparticles and subjected to enzymatic mineralization with calcium phosphate (CaP). The method of manufacturing CaCO3 microparticles was optimized. The EGCG-loaded microparticles were smaller than those unloaded, and the release of EGCG was prolonged for up to 14 days, as shown by the Folin-Ciocalteu test. The particles reduced the viability of the MG-63 cells as compared to the control. However, when they were loaded with EGCG, their cytotoxicity was reduced. The particles were suspended in a GG/Gel hydrogel containing alkaline phosphatase (ALP), soaked in calcium glycerophosphate (CaGP) solution to create CaP deposits, and submitted to freeze-drying, in order to produce a porous scaffold. The microstructure of the scaffolds was characterized by optical and scanning electron microscopy and showed that the size of the pores corresponds to that of the spongy bone. In vitro tests with MG-63 cells confirmed that mineralized scaffolds support cell adhesion and growth to a higher extent than nonmineralized ones.
Źródło:
Engineering of Biomaterials; 2022, 25, 166; 12--21
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Curcumin delivery systems as dressing components for the treatment of diabetic foot ulcers: test on macrophages polarization
Autorzy:
Wójcik, Karolina
Kusibab, Anna
Sousa, Ana Beatriz
Barbosa, Judite Novais
Pamuła, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/27324039.pdf
Data publikacji:
2023
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
curcumin
immunomodulation
inflammation
macrophage polarization
polymeric microparticles
wound healing
makrofagi
polaryzacja
biomateriały
Opis:
The natural wound healing process consists of four basic phases: homeostasis, inflammation, proliferation, and remodelling. Macrophages play an important role in the body’s response to biomaterials, as they are modulators of the wound healing process and can polarize into different phenotypes capable of inducing both deleterious and beneficial effects on tissue repair. Curcumin (CU) is known for its anti-inflammatory properties and has the potential to treat diabetic foot ulcers, but it should be delivered to wounds in a controlled manner. In this study, the encapsulation of curcumin in polymeric microparticles based on poly(sebacic anhydride) (PSA) was developed using an emulsification method. PSA-based microparticles containing different concentrations of CU were obtained: 0% weight (wt). CU (unloaded microparticles), 5, 10, and 20 wt% CU. CU encapsulation efficiency and loading were determined using a fluorescence-based calibration curve method and semi-quantitative Fourier-transform infrared spectroscopy (FTIR) analysis. The potential cytotoxicity of the obtained biomaterials in contact with primary human macrophages and their susceptibility to polarization from the M1 (pro-inflammatory) phenotype to the M2 (antiinflammatory) phenotype were evaluated. The morphology of cells cultured in contact with polymeric microparticles was evaluated using phalloidin red and 4′,6-diamidino2-phenylindole (DAPI) staining. Macrophage phenotype was assessed using flow cytometry. The obtained biomaterials showed no cytotoxic effect on primary human macrophages. Flow cytometry studies showed enhanced polarization of macrophages into anti-inflammatory M2 phenotype when exposed to microparticles loaded with CU and CU powder as compared to unloaded microparticles
Źródło:
Engineering of Biomaterials; 2023, 26, 168; 15--24
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cytocompatibility tests of Poly(diol citrate) polymers in contact with adipose tissue derived stem cells for vascular tissue engineering purposes
Autorzy:
Flis, Agata
Travnickova, Martina
Koper, Filip
Bacakova, Lucie
Kasprzyk, Wiktor
Pamuła, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/283939.pdf
Data publikacji:
2019
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Źródło:
Engineering of Biomaterials; 2019, 22, no.153; 76
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Degradation studies of poly(diol citrates) for vascular tissue engineering purposes
Autorzy:
Kwiecień, Konrad
Flis, Agata
Koper, Filip
Kasprzyk, Wiktor
Pamuła, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/1844901.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
tissue engineering
degradation
materials
inżynieria tkankowa
degradacja
materiały
Źródło:
Engineering of Biomaterials; 2020, 23, 158 spec. iss.; 65
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of polyester coatings on poly(ethylene terephtalate) prostheses for vascular tissue engineering
Autorzy:
Flis, Agata
Calik, Laura
Koper, Filip
Kasprzyk, Wiktor
Pamuła, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/1844751.pdf
Data publikacji:
2021
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Źródło:
Engineering of Biomaterials; 2021, 24, 163; 82
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hydrogel modification with the use of CaCO3 microparticles enriched with antibacterial peptides
Autorzy:
Reczyńska, Katarzyna
Pamuła, Elżbieta
Guimier, Eugénie
Powiązania:
https://bibliotekanauki.pl/articles/284780.pdf
Data publikacji:
2019
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Źródło:
Engineering of Biomaterials; 2019, 22, no.153; 88
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hydrogel scaffolds for medical applications obtained by indirect 3D-printing method
Autorzy:
Pietryga, Krzysztof
Pamuła, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/284894.pdf
Data publikacji:
2019
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Źródło:
Engineering of Biomaterials; 2019, 22, no.153; 93
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Inhalable drug delivery system based on poly(sebacic acid) and azithromycin for the treatment of bacterial infections
Autorzy:
Reczyńska-Kolman, Katarzyna
Kwiecień, Konrad
Knap, Karolina
Niewolnik, Daria
Kazek-Kęsik, Alicja
Płonka, Joanna
Jaszcz, Katarzyna
Pamuła, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/1844757.pdf
Data publikacji:
2021
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Źródło:
Engineering of Biomaterials; 2021, 24, 163; 86
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Manufacturing and characterization of gellan gum – zinc oxide composites as potential biomaterials for wound treatment
Autorzy:
Macyk, Alicja
Kusibab, Anna
Pamuła, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/27324040.pdf
Data publikacji:
2023
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
gellan gum
zinc oxide nanoparticles
zinc oxide
microparticles
wound healing
L929 fibroblasts
cynk
fibroblasty
biomateriały
Opis:
This study aimed to produce gellan gum-based hydrogels with the addition of zinc oxide as a potential dressing material. Hydrogels with ZnO concentrations of 0.01%, 0.02% and 0.04% were prepared, micrometric and nanometric ZnO particles were used, and a CaCl2 crosslinker was added to one part of the samples. All samples (14 types) produced by the freeze drying method were characterized with high swelling properties (>2000%), what is important to ensure the absorption of exudates from wounds. Samples with ZnO particles cross-linked with CaCl2 lost less mass after incubation in aqueous media and were characterized by better dimensional stability than those without crosslinking. The pH of the extracts of the samples containing ZnO particles was more neutral (pH 7.0-7.6) than that of the control gellan gum samples (pH of 5.5-6.1). The zinc release from cross-linked samples was twice as high for those containing nanometric particles than for micrometric particles (1.94 ± 0.04 mg/l and 0.93 ± 0.02, respectively). Relatively large amounts of released zinc species in the case of samples containing ZnO nanoparticles are promising in the context of the antibacterial properties and treatment of infected wounds. A lower amount of zinc released from samples with ZnO microparticles could be sufficient to prevent the development of the infection. Furthermore, both materials show satisfactory cytocompatibility with L929 fibroblasts, as shown by Alamar blue and live/dead viability tests, making them prospective candidates for wound healing
Źródło:
Engineering of Biomaterials; 2023, 26, 168; 2--8
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modification of titanium implant surface for animals
Autorzy:
Kazek-Kęsik, Alicja
Sadkowska, Alicja
Reczyńska-Kolman, Katarzyna
Kalemba-Rec, Izabela
Śmiga-Matuszowicz, Monika
Pamuła, Elżbieta
Simka, Wojciech
Powiązania:
https://bibliotekanauki.pl/articles/1844839.pdf
Data publikacji:
2021
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Źródło:
Engineering of Biomaterials; 2021, 24, 163; 16
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimizing manufacturing conditions of polymer microspheres as cell carriers for modular tissue engineering
Autorzy:
Mielan, Bartosz
Pamuła, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/1844978.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
modular tissue engineering
microspheres
cell culture
oil-in-water emulsification
poly(L-lactide-co-glycolide) (PLGA)
Opis:
Microspheres (MS) made of biostable polymer, namely polystyrene, have been used as substrates for cell culture enabling rapid cell expansion in dynamic conditions. However, due to non-resorbability, polystyrene (PS) MS when repopulated with cells cannot be directly used in tissue engineering. Our concept was to produce MS from resorbable polymer – poly(L-lactide- -co-glycolide) (PLGA) as a support for adherent cells, e.g. osteoblasts. We hypothesize that such MS can be applied to the injured site to act as cell carriers or as modules for modular tissue engineering (MTE). In this article, we present the results of optimizing the PLGA MS manufacturing conditions via oil-in-water emulsification. Due to such a technique, MS with the required size, size distribution and properties suitable for cell culturing can be obtained. Three parameters of the oil-in-water emulsification were examined: the stirring speed of a water phase during MS manufacturing, the surfactant concentration, i.e. poly(vinyl alcohol) (PVA) in a water phase and concentration of PLGA in dichloromethane (DCM) as an oil phase. The results proved that the 7.5% PLGA concentration in DCM solution as an oil phase, the 0.5-2% concentration of PVA solution as a water phase and the stirring speed of water phase of 1000 rpm provided MS with the 160 μm mean diameter, which is suitable for cell culture. Moreover, the developed sieving and cleaning procedures were efficient to collect MS with the mean diameter of 280 μm, the more coherent size distribution and the ability to sink in the cell culture medium. The presence on the bottom of cell culture wells is crucial for MTE.
Źródło:
Engineering of Biomaterials; 2020, 23, 156; 2-9
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phenolic plant extract enrichment of enzymatically mineralized hydrogels
Autorzy:
Douglas, Timothy E.L
Lopez-Heredia, Marco A.
Pułczyńska, Aleksandra
Łapa, Agata
Pietryga, Krzysztof
Schaubroeck, David
Santos, Sónia A.O.
Pais, Adriana
Brackman, Gilles
Schamphelaere, Karel de
Samal, Sangram Keshari
Keppler, Julia K.
Bauer, Jonas L
Chai, Feng
Blanchemain, Nicolas
Coenye, Tom
Pamuła, Elżbieta
Skirtach, Andre G
Powiązania:
https://bibliotekanauki.pl/articles/284057.pdf
Data publikacji:
2019
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
hydrogel
mineralization
polyphenols
antibacterial
epigallocatechin gallate
gellan gum
Opis:
Hydrogel mineralization with calcium phosphate (CaP) and antibacterial activity are desirable for applications in bone regeneration. Mineralization with CaP can be induced using the enzyme alkaline phosphatase (ALP), responsible for CaP formation in bone tissue. Incorporation of polyphenols, plant-derived bactericidal molecules, was hypothesized to provide antibacterial activity and enhance ALP-induced mineralization. Three phenolic rich plant extracts from: (i) green tea, rich in epigallocatechin gallate (EGCG) (herafter referred to as EGCG-rich extract); (ii) pine bark and (iii) rosemary were added to gellan gum (GG) hydrogels and subsequently mineralized using ALP. The phenolic composition of the three extracts used were analyzed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MSn). EGCG-rich extract showed the highest content of phenolic compounds and promoted the highest CaP formation as corroborated by dry mass percentage meassurements and ICP-OES de-termination of mass of elemental Ca and P. All three extracts alone exhibited antibacterial activity in the following order EGCG-rich > PI > RO, respectively. However, extract-loaded and mineralized GG hydro-gels did not exhibit appreciable antibacterial activity by diffusion test. In conclusion, only the EGCG-rich extract promotes ALP-mediated mineralization.
Źródło:
Engineering of Biomaterials; 2019, 22, 149; 2-9
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Poly(L-lactide-co-glycolide) membranes modified with RGD-poly-(2-oxazoline) for biomedical application
Autorzy:
Tryba, Anna Maria
Krok-Borkowicz, Małgorzata
Piergies, Natalia
Paluszkiewicz, Czesława
Frączyk, Justyna
Kolesińska, Beata
Pamuła, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/1844845.pdf
Data publikacji:
2021
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Źródło:
Engineering of Biomaterials; 2021, 24, 163; 44
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Poly(sebacic anhydride) microparticles loaded with curcumin for pulmonary purposes
Autorzy:
Kwiecień, Konrad
Reczyńska-Kolman, Katarzyna
Niewolik, Daria
Jaszcz, Katarzyna
Pamuła, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/2058396.pdf
Data publikacji:
2021
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
poly(sebacic anhydride)
curcumin
drug delivery system
pulmonary infections
polymeric microparticles
lekarstwa
dystrybucja
infekcje pulmonologiczne
Opis:
Microparticles (MPs) made of fast biodegrading biomaterials, loaded with drugs, are considered a superior treatment method for pulmonary infections. One of the promising biomaterials for obtaining such a drug delivery system (DDS) is poly(sebacic anhydride) (PSA) due to its favourable degradation kinetics and mechanism. In this paper, we present a study of manufacturing MPs from PSA loaded with curcumin (CU) for pulmonary purposes. MPs were manufactured by oil-in-water emulsification; their morphology and size distribution were evaluated using optical microscopy, while the encapsulation efficiency and drug loading were obtained by the fluorometric assay. The cytotoxicity of the MPs, both the empty ones and loaded with CU, was analysed by in vitro tests with BEAS-2B human lung epithelial cells. To this end, metabolic activity by AlamarBlue assay and fluorescent staining (DAPI/ eosin) of the cells were performed. The MPs produced were round, regular in shape with diameters in the range of 1-5 µm and of yellow colour originating from CU. The CU encapsulation efficiency ranged from 42% to 55% and decreased with a higher CU ratio. The drug loading ranged from 4% to 11% and increased at a higher CU ratio. Both empty and CU-loaded MPs did not show a cytotoxic effect at concentrations up to 10 µg/ml.
Źródło:
Engineering of Biomaterials; 2021, 24, 162; 7--12
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 20

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