Autor: Ortiz, J P A - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: A rapid system for studying foreign gene expression in wheat [Triticum aestivum L.]
Autorzy:: Ortiz, J P A
Ravizzini, R A
Morata, M M
Vallejos, R H
Powiązania:: https://bibliotekanauki.pl/articles/2046807.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Buck Ombu cultivar
gene expression
wheat
leaf segment
transgenic plant
Triticum aestivum
embryo
Opis:: The present work describes a rapid and easy way to study foreign gene expression in wheat through microparticle bombardment. Transient expression of the GUS reporter gene was evaluated in different tissues of the cultivar Buck Ombú. Transformation was carried out employing a helium gun microparticle accelerator and several plasmids. Embryogcnic calli, immature embryos and immature inflorescences showed a higher number of transformed cells per Petri dish. Basal leaf segments were the least efficient. When vectors were tested in scutellar tissue of immature embryos, the maize ubiquitin promoter (Ubil) produced the highest level of transient GUS expression, followed by the alcohol dehydrogenase promoter of maize plus its first intron (Adh1 Adh-intron 1) and the sunflower ubiquitin promoter. The CaMV35S promoter was the least effective. These results were in agreement with previous reports carried out in cell suspensions or embryogenic calli and indicate that wheat immature embryos and immature inflorescences are suitable for rapid test of promoter sequences or transformation conditions. Moreover, efficient transformation of these explants could help in the stable transformation of agronomically important genotypes.
Źródło:: Journal of Applied Genetics; 1997, 38, 2; 123-130
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Unconformity surfaces in pelagic carbonate environments : A case from the Middle Bathonian of the Betic Cordillera, SE Spain
Autorzy:: Nieto, L. M.
Rodríguez-Tovar, F. J.
Molina, J. M.
Reolid, M.
Ruiz-Ortiz, P. A.
Powiązania:: https://bibliotekanauki.pl/articles/191710.pdf
Data publikacji:: 2014
Wydawca:: Polskie Towarzystwo Geologiczne
Tematy:: Unconformity
pelagic swell
softground
firmground
hardground
Middle-Late Jurassic
Betic External Zone
Opis:: Integrative studies of sedimentary and palaeontological topics for unconformity surfaces are useful in basin analysis. A middle Bathonian unconformity surface in the Ammonitico Rosso facies cropping out in the La Mola Unit (Subbetic Domain, Betic External Zone) was studied by integrating ichnological, palaeotectonic, and sedimentological analyses to decipher sea-level, tectonic, and palaeogeographic conditions during its development. The trace-fossil assemblage mainly consists of the Glossifungites (Thalassinoides, Arenicolites, and Gastrochaenolites) and Trypanites ichnofacies elements. Probable Ophiomorpha represents previous softground stages, Thalassinoides and Arenicolites were formed in firmground, Gastrochaenolites reflects an evolved firmground or early hardground, and Trypanites can be attributed to an incipient hardground. The degree of firmness, relative sea-level position, and continuity of deposition were related. The softground stage corresponds to a fall in relative sea level and continued deposition. The firmground (semi-consolidated substrate) probably reflects an extremely low sea level characterized by non-deposition, whereas the incipient hardground stage indicates an initial phase of relative sea-level rise, with an increase in marine current energy. The presence of two neptunian dyke systems reflects significant tectonic activity related to the transtensional deformation that affected the South Iberian Palaeomargin. Lateral variations in sedimentological and ichnological features recorded at similar discontinuity surfaces in nearby areas were considered and related to differences in bottom topography, with associated changes in sedimentation, and to the variable duration of the hiatus.
Źródło:: Annales Societatis Geologorum Poloniae; 2014, 84, 3; 281-295
0208-9068
Pojawia się w:: Annales Societatis Geologorum Poloniae
Dostawca treści:: Biblioteka Nauki