Autor: Ondřej, Vladan - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Changes of DNA methylation and hydroxymethylation in plant protoplast cultures
Autorzy:: Moricová, Pavla
Ondřej, Vladan
Navrátilová, Božena
Luhová, Lenka
Powiązania:: https://bibliotekanauki.pl/articles/1039600.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: Brassica oleracea
DNA hydroxymethylation
Cucumis sativus
protoplasts
DNA methylation
Opis:: Cytosine methylation patterns in higher eukaryotes are important in gene regulation. Along with 5-methylcytosine (5-mC), a newly discovered constituent of mammalian DNA, 5-hydroxymethylcytosine (5-hmC), is the other modified base in higher organisms. In this study we detected 5-hmC in plant protoplast DNA and demonstrated its increasing content during the first 72 hrs. of protoplast cultivation. In contrast to 5-hmC, the amount of 5-mC decreased during protoplast cultivation. It was also found that 5-hmC did not primarily arise as a product of oxidative DNA damage following protoplast culture.
Źródło:: Acta Biochimica Polonica; 2013, 60, 1; 33-36
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

Artykuł

Skocz do pozycji: 2.

Tytuł:: The role of actin and microtubule networks in plasmid DNA intracellular trafficking
Autorzy:: Ondřej, Vladan
Lukášová, Emilie
Falk, Martin
Kozubek, Stanislav
Powiązania:: https://bibliotekanauki.pl/articles/1041059.pdf
Data publikacji:: 2007
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: cytoplasmic trafficking
microtubules
plasmid DNA-lipid complexes
actin filaments
Opis:: This work is focused on the function of the microtubule and actin networks in plasmid DNA transport during liposomal transfection. We observed strong binding of plasmid DNA-lipid complexes (lipoplexes) to both networks and directional long-range motion of these lipoplexes along the microtubules. Disruption of either of these networks led to the cessation of plasmid transport to the nucleus, a decreased mobility of plasmids, and accumulation of plasmid DNA in large aggregates at the cell periphery. Our findings show an indispensable but different role of both types of cytoskeleton, actin and microtubular, in the processes of gene delivery.
Źródło:: Acta Biochimica Polonica; 2007, 54, 3; 657-663
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

Artykuł

Skocz do pozycji: 3.

Tytuł:: Intranuclear trafficking of plasmid DNA is mediated by nuclear polymeric proteins lamins and actin
Autorzy:: Ondřej, Vladan
Lukášová, Emilie
Krejčí, Jana
Kozubek, Stanislav
Powiązania:: https://bibliotekanauki.pl/articles/1040744.pdf
Data publikacji:: 2008
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: plasmid DNA
DNA double-strand breaks
nuclear actin
lamin A/C
Opis:: Functions of nuclear polymeric proteins such as lamin A/C and actin in transport of plasmid DNA were studied. The results show that the lamina plays an important role in plasmid DNA's entry into the cell nucleus from the cytoplasm. Selective disruption of lamin A/C led to a halt in plasmid DNA transport through the nuclear envelope. Inside the nucleus, plasmid DNA was frequently localized at sites with impaired genome integrity, such as DNA double-strand breaks (DSBs), occurring spontaneously or induced by ionizing radiation. Polymeric actin obviously participates in nuclear transport of plasmid DNA, since inhibition of actin polymerization by latrunculin B disturbed plasmid transport inside the cell nucleus. In addition, precluding of actin polymerization inhibited plasmid co-localization with newly induced DSBs. These findings indicate the crucial role of polymeric actin in intranuclear plasmid transport.
Źródło:: Acta Biochimica Polonica; 2008, 55, 2; 307-315
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

Artykuł

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