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Wyszukujesz frazę "Olejnik-Schmidt, Agnieszka" wg kryterium: Autor

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    Wyświetlanie 1-3 z 3
    Tytuł:
    SpaCBA sequence instability and its relationship to the adhesion efficiency of Lactobacillus casei group isolates to Caco-2 cells
    Autorzy:
    Markowicz, Corinna
    Olejnik-Schmidt, Agnieszka
    Borkowska, Monika
    Schmidt, Marcin
    Powiązania:
    https://bibliotekanauki.pl/articles/1039301.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Lactobacillus
    adhesion
    spaCBA
    Opis:
    The ability to adhere to enterocytes is one of the key features of probiotics. This process involves a number of factors, among which the important role of pili was demonstrated. Some Lactobacillus species are confirmed to have heterotrimeric spaCBA type pili. The aim of this study was to identify spaCBA pili in strains of selected Lactobacillus spp. and assess the impact of their presence and sequence polymorphism on the adhesion of these strains to enterocytes. Total 20 bacterial strains of L. rhamnosus, L. casei and L. paracasei were tested. The presence of pilus specific proteins coding genes spaA, spaB and spaC was verified by PCR in order to identify the presence of sequence polymorphism in the genes possibly affecting the structure of the spaCBA pilus. To correlate spaCBA polymorphism to adhesion capability the adhesion assay was carried out using Caco-2 cell line. The effectiveness of the adhesion was measured using a scintillation counter. The Lactobacillus strains analyzed showed the adhesion to Caco-2 enterocytes capability from 0.6% to 19.6%. The presence of spaCBA pili is a factor increasing the adhesion efficiency of Lactobacillus spp. to Caco-2 enterocytes. Lack of these structures on the surface of bacterial cells results in the reduction in adhesion efficiency, indicating its important role in the adhesion process. But not in all cases the correlation between the presence of protein spaCBA structures and adhesion efficiency was observed, what may indicate the important role of other factors in adhesion of analyzed strains to Caco-2 cells.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 2; 341-347
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impact of DNA microarray data transformation on gene expression analysis - comparison of two normalization methods
    Autorzy:
    Schmidt, Marcin
    Handschuh, Luiza
    Zyprych, Joanna
    Szabelska, Alicja
    Olejnik-Schmidt, Agnieszka
    Siatkowski, Idzi
    Figlerowicz, Marek
    Powiązania:
    https://bibliotekanauki.pl/articles/1039855.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    microarray
    data normalization
    enterocyte
    transcriptome analysis
    probiotic
    adhesion
    gene expression profiling
    Opis:
    Two-color DNA microarrays are commonly used for the analysis of global gene expression. They provide information on relative abundance of thousands of mRNAs. However, the generated data need to be normalized to minimize systematic variations so that biologically significant differences can be more easily identified. A large number of normalization procedures have been proposed and many softwares for microarray data analysis are available. Here, we have applied two normalization methods (median and loess) from two packages of microarray data analysis softwares. They were examined using a sample data set. We found that the number of genes identified as differentially expressed varied significantly depending on the method applied. The obtained results, i.e. lists of differentially expressed genes, were consistent only when we used median normalization methods. Loess normalization implemented in the two software packages provided less coherent and for some probes even contradictory results. In general, our results provide an additional piece of evidence that the normalization method can profoundly influence final results of DNA microarray-based analysis. The impact of the normalization method depends greatly on the algorithm employed. Consequently, the normalization procedure must be carefully considered and optimized for each individual data set.
    Źródło:
    Acta Biochimica Polonica; 2011, 58, 4; 573-580
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The HPV16 E2 transcriptional regulator mode of action depends on the physical state of the viral genome
    Autorzy:
    Schmidt, Marcin
    Olejnik, Agnieszka
    Goździcka-Józefiak, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1041324.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    E2
    chromatin
    transcription
    HPV
    retinol
    steroid hormones
    LCR
    Opis:
    Human papillomavirus (HPV) infection is a major risk factor for the development of cervical cancer. The HPV-induced immortalization of epithelial cell usually requires integration of the viral DNA into the host cell genome. The integration event causes disruption of the E2 gene and this is followed by overexpression of the E6 and E7 oncoproteins. The E2 protein is a transcription factor that regulates expression of the E6 and E7 oncoproteins by binding to four sites within the viral long control region. We used an in vitro cell culture model to explore the role of the E2 protein in the transcriptional control of the HPV16 long control region. Employing transient and stable transfection experiments we simulated the episomal and integrated states of the viral genome, respectively. We show that the E2 protein up-regulates E6/E7 transcription from episomal DNA but represses it in the case of integrated DNA. The activator function of the E2 protein seems to counteract the repressive chromatin structure formed over episomal DNA. Steroid hormones and retinol also modulate oncogene transcription differently depending on the physical structure of the viral DNA. Our data suggest regulatory mechanisms involving interactions between the E2 protein and nuclear hormone receptors.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 4; 823-832
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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