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Wyszukujesz frazę "Olchawa-Pajor, Monika" wg kryterium: Autor


Wyświetlanie 1-3 z 3
Tytuł:
Expression of three diadinoxanthin de-epoxidase genes of Phaeodacylum tricornutum in Escherichia coli Origami b and BL21 strain
Autorzy:
Bojko, Monika
Olchawa-Pajor, Monika
Tuleja, Urszula
Kuczyńska, Paulina
Strzałka, Wojciech
Latowski, Dariusz
Strzałka, Kazimierz
Powiązania:
https://bibliotekanauki.pl/articles/1039507.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
diadinoxanthin cycle
marine diatoms
violaxanthin
de-epoxidation
Opis:
In the diadinoxanthin cycle the epoxy group is removed from diadinoxanthin and diatoxanthin is created. This conversion takes place e.g. in diatoms with the involvement of the enzyme diadinoxanthin de-epoxidase. In one of the diatom species, Phaeodactylum tricornutum (CCAP 1055/1 strain with genome sequenced) three de-epoxidase genes (PtVDE, PtVDL1, PtVDL2) have been identified, but only one of them (PtVDE) corresponds to violaxanthin de-epoxidase, an enzyme which is commonly found in higher plants. In these studies, the expression of two de-epoxidase genes of another Phaeodactylum tricornutum strain (UTEX 646), which is commonly used in diatom studies, were obtained in Origami b and BL21 E. coli strains. The molecular masses of the mature proteins are about 49 kDa and 60 kDa, respectively, for VDE and VDL2. Both enzymes are active with violaxanthin as a substrate.
Źródło:
Acta Biochimica Polonica; 2013, 60, 4; 857-860
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Temperature effect on growth, and selected parameters of Phaeodactylum tricornutum in batch cultures
Autorzy:
Bojko, Monika
Brzostowska, Klaudia
Kuczyńska, Paulina
Latowski, Dariusz
Olchawa-Pajor, Monika
Krzeszowiec, Weronika
Waloszek, Andrzej
Strzałka, Kazimierz
Powiązania:
https://bibliotekanauki.pl/articles/1039508.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Phaeodactylum tricornutum
diatoms
chlorophylls
cell size
photosynthesis
Fv/Fm
Opis:
The effect of optimal and stress temperatures on the growth kinetics of the Phaeodactylum tricornutum CCAP/1055/1 strain (a model diatom with a known genome sequence) in batch cultures was examined. The analysis of the obtained results showed two phases of culture growth. There were significant positive correlations between OD increase of chlorophyll a chlorophyll c and protein concentration at different temperatures. The Fv/Fm parameter achieved a maximum level on the 6th or 7th day and then decreased to the values registered on the first day of observation. Genetic material undergoes gradual degradation 10 days after inoculation. The size of the cells was invariable.
Źródło:
Acta Biochimica Polonica; 2013, 60, 4; 861-864
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zeaxanthin epoxidation - an in vitro approach
Autorzy:
Kuczyńska, Paulina
Latowski, Dariusz
Niczyporuk, Sylvia
Olchawa-Pajor, Monika
Jahns, Peter
Gruszecki, Wiesław
Strzałka, Kazimierz
Powiązania:
https://bibliotekanauki.pl/articles/1039789.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Arabidopsis thaliana mutants npq1
model system
npq2
xanthophyll cycle
zeaxanthin epoxidation
Opis:
Zeaxanthin epoxidase (ZE) is an enzyme operating in the violaxanthin cycle, which is involved in photoprotective mechanisms. In this work model systems to study zeaxanthin (Zx) epoxidation were developed. Two assay systems are presented in which epoxidation of Zx was observed. In these assays two mutants of Arabidopsis thaliana which have active only one of the two xanthophyll cycle enzymes were used. The npq1 mutant possesses an active ZE and is thus able to convert Zx to violaxanthin (Vx) but the violaxanthin de-epoxidase (VDE) is inactive, so that Vx cannot be converted to Zx. The other mutant, npq2, possesses an active VDE and can convert exogenous Vx to Zx under strong light conditions but reverse reaction is not possible. The first assay containing thylakoids from npq1 and npq2 mutants of A. thaliana gave positive results and high efficiency of epoxidation reaction was observed. The amount of Zx was reduced by 25%. To optimize high efficiency of epoxidation reaction additional factors facilitating both fusion of the two types of thylakoids and incorporation of Zx to their membranes were also studied. The second kind of assay contained npq1 mutant thylakoids of A. thaliana supplemented with exogenous Zx and monogalactosyldiacylglycerol (MGDG). Experiments with different proportions of Zx and MGDG showed that their optimal ratio is 1:60. In such system, due to epoxidation, the amount of Zx was reduced by 38% of its initial level. The in vitro systems of Zx epoxidation described in this paper enable analysis some properties of the ZE without necessity of its isolation.
Źródło:
Acta Biochimica Polonica; 2012, 59, 1; 105-107
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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