- Tytuł:
- Anti-epileptic drugs inhibit viability of synoviocytes in vitro
- Autorzy:
-
Parada-Turska, J.
Nowicka-Stazka, P.
Majdan, M.
Jablonski, M.
Turski, W.A.
Rzeski, W. - Powiązania:
- https://bibliotekanauki.pl/articles/51168.pdf
- Data publikacji:
- 2013
- Wydawca:
- Instytut Medycyny Wsi
- Opis:
- Introduction and objective: The hyperplasia of synovial fibroblasts is considered to be essential for the evolution of joint destruction in rheumatoid arthritis (RA). Previously, we reported that anti-rheumatic drugs, both COX inhibitors and disease-modifying anti-rheumatic drugs inhibit proliferation of synoviocytes in vitro. The presented study investigates the effect of anti-epileptic drugs on the viability and proliferation of synovial fibroblasts in vitro. Methods: Experiments were conducted on human synoviocytes derived from an RA patient and rabbit synoviocytes cell line HIG-82. Cell proliferation and viability were assessed by means of BrdU assay and MTT assay, respectively. The IC50 value (the concentration of drug necessary to induce 50% inhibition) together with confidence limits was calculated. Results: Carbamazepine inhibited proliferation of human fibroblasts and viability of HIG-82 with IC50 values of 86 μM and 82 μM, respectively. Diphenylhydantoin, valproate and phenobarbital inhibited viability of HIG-82 cells with the IC50 values of 110, 500 and 1031 μM, respectively. Conclusion: Based on these findings, it can be suggested that anti-epileptic drugs may have a disease-modifying effect on rheumatoid synovial proliferation.
- Źródło:
-
Annals of Agricultural and Environmental Medicine; 2013, 20, 3
1232-1966 - Pojawia się w:
- Annals of Agricultural and Environmental Medicine
- Dostawca treści:
- Biblioteka Nauki
Artykuł