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    Wyświetlanie 1-4 z 4
    Tytuł:
    Comparison of the utility of five commercial kits for extraction of DNA from Aspergillus fumigatus spores
    Autorzy:
    Nawrot, Urszula
    Wlodarczyk, Katarzyna
    Wrobel, Magdalena
    Wasik, Anita
    Dobosz, Tadeusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1040325.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    DNA extraction
    aspergillosis
    Aspergillus
    Opis:
    The aim of this study was to compare the efficiency of DNA extraction from water as well as from blood samples spiked with A. fumigatus spores, using selected commercial kits. Extraction of DNA according to manufacturer's protocols was preceded by blood cells lysis and disruption of fungal cells by enzymatic digestion or bead beating. The efficiency of DNA extraction was measured by PCR using Aspergillus-specific primers and SYBR Green I dye or TaqMan probes targeting 28S rRNA gene. All methods allowed the detection of Aspergillus at the lowest tested density of water suspensions of spores (101 cells/ml). The highest DNA yield was obtained using the ZR Fungal/Bacterial DNA kit, YeastStar Genomic DNA kit, and QIAamp DNA Mini kit with mechanical cell disruption. The ZR Fungal/Bacterial DNA and YeastStar kits showed the highest sensitivity in examination of blood samples spiked with Aspergillus (100 % for the detection of 102 spores and 75 % for 101 spores). Recently, the enzymatic method ceased to be recommended for examination of blood samples for Aspergillus, thus ZR Fungal/Bacterial DNA kit and QIAamp DNA Mini kit with mechanical cell disruption could be used for extraction of Aspergillus DNA from clinical samples.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 4; 567-571
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Examination of cyp51A and cyp51B expression level of the first Polish azole resistant clinical Aspergillus fumigatus isolate
    Autorzy:
    Brillowska-Dąbrowska, Anna
    Mroczyńska, Martyna
    Nawrot, Urszula
    Włodarczyk, Katarzyna
    Kurzyk, Ewelina
    Powiązania:
    https://bibliotekanauki.pl/articles/1038929.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Aspergillus fumigatus
    azole resistance
    cyp51A
    cyp51B
    Opis:
    Aspergillus fumigatus is one of the most prevalent airborne fungal pathogens causing infections worldwide. Most A. fumigatus strains are susceptible to azoles, which are administered as the first line therapeutics. However, during last decade the acquired resistance to triazoles by these species has been described. There is a number of publications concerning the examination of clinical A. fumigatus strains from different countries, however there has been no report from Poland. Here, we describe for the first time, an examination of cyp51A and cyp51B expression level of 11 clinical A. fumigatus strains isolated during 2007-2014 period from the collection of Medical University in Wrocław. Their susceptibility to itraconazole, voriconazole and posaconazole has been examined. The MIC values of triazoles for one of the examined isolates were respectively: > 8 mg/L for itraconazole, 2 mg/L for voriconazole and 0.5 mg/L for posaconazole. The cyp51A gene with its promoter region of all isolates was sequenced. It was found that the resistant isolate harbors the TR34/L98H mutation in the cyp51A gene and when cultured on media supplemented with voriconazole exhibits overexpression of both, cyp51A and cyp51B genes. The level of cyp51A gene expression was about 50 times higher than cyp51B.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 4; 837-839
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Relation of the polymorphism of cyp51A sequence and the susceptibility of Aspergillus fumigatus isolates to triazoles determined by commercial gradient test (Etest) and by reference methods
    Autorzy:
    Nawrot, Urszula
    Sulik-Tyszka, Beata
    Kurzyk, Ewelina
    Mroczyńska, Martyna
    Włodarczyk, Katarzyna
    Wróblewska, Marta
    Basak, Grzegorz
    Brillowska-Dąbrowska, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1038548.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Aspergillus fumigatus
    triazole resistance
    susceptibility testing
    Etest
    cyp51A sequence
    Opis:
    The aim of this study was to evaluate the accuracy of commercial gradient test (Etest) in the detection of triazole resistant Aspergillus fumigatus isolates using reference microdilution methods and the analysis of sequences of the cyp 51A gene. The study was performed on twenty clinical isolates which were identified as Aspergillus fumigatus based on the DNA sequences of the ITS1-2 fragment of ribosomal DNA and the β-tubulin gene, out of them seventeen isolates showed wild-type cyp51A sequence and three were positive for the mutation TR34/L98H. All isolates were tested for the susceptibility to itraconazole (ITZ), voriconazole (VOR) and posaconasole (POS) using microdilution methods, according to EUCAST and CLSI protocols, as well as using Etest. The results of microdilution and Etests were analysed separately according to clinical breakpoints (CBP) defined by EUCAST version 7.0 and epidemiological cut off values (ECV). Etest as well as reference methods excellently recognised the WT isolates, which were susceptible to all tested triazoles, regardless of the method and CBP or ECV criteria used. The Etest recognized three non-WT isolates as resistant or intermediately sensitive to ITZ and POS and one as resistant to VOR. The categorical concordance between Etests and EUCAST and Etests and the CLSI method ranged from 90 to 100%. The interpretation of the results obtained from routine A. fumigatus Etests requires great caution. The use of the confirmative examinations with reference AST methods as well as with molecular tests is recommended.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 4; 631-634
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analiza zmienności epigenetycznej indukowanej stresem suszy oraz ocena jej stabilności transgeneracyjnej w aspekcie tolerancji jęczmienia na stres niedoboru wody
    The analysis of the epigenetic diversity induced by drought stress and the assessment of its transgenerational stability with respect to the drought stress tolerance in barley
    Autorzy:
    Kwaśniewski, Mirosław
    Chwiałkowska, Karolina
    Nowakowska, Urszula
    Nawrot, Małgorzata
    Jelonek, Janusz
    Skawińska-Zydroń, Grażyna
    Bielska, Agnieszka
    Powiązania:
    https://bibliotekanauki.pl/articles/2199312.pdf
    Data publikacji:
    2019-11-30
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    epigenetyka
    jęczmień
    NGS
    stres
    susza
    zmienność transgeneracyjna
    Źródło:
    Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2019, 286; 95-99
    0373-7837
    2657-8913
    Pojawia się w:
    Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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