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    Wyświetlanie 1-4 z 4
    Tytuł:
    Production and characterization of egg yolk antibody (IgY) against recombinant VP8 - S2 antigen
    Autorzy:
    Nasiri, K.
    Nassiri, M.R.
    Tahmoorespur, M.
    Haghparast, A.
    Zibaee, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/30350.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Bovine Rotavirus and Bovine Coronavirus are the most important causes of diarrhea in newborn calves and in some other species such as pigs and sheep. VP8 subunit of rotavirus is the major determinant of the viral infectivity and neutralization. Spike glycoprotein of coronavirus is responsible for induction of neutralizing antibody response. Studies showed that immunoglobulin of egg yolk (IgY) from immunized hens has been identified to be a convenient source for specific antibodies for using in immunotherapy and immunodiagnostic to limit the infections. In this study, chimeric VP8-S2 gene was designed using by computational techniques. The chimeric VP8-S2 gene was cloned and sub-cloned into pGH and pET32a (+) vectors. Then, recombinant pET32a-VP8-S2 vector was transferred into E. coli BL21 CodonPlus (DE3). The expressed protein was purified by Ni-NTA chromatography column. Hens were immunized with the purified VP8-S2 protein three times. IgY was purified from egg yolks using polyethylene glycol precipitation method. Activity and specificity of anti-VP8-S2 IgY were detected by dot-blotting, Western-blotting and indirect ELISA. We obtained anti-VP8-S2 IgY by immunizing hens with the recombinant VP8-S2 protein. The anti-VP8-S2 IgY was showed to bind specifically to the chimeric VP8-S2 protein by dot-blotting, Western-blotting analyses and indirect ELISA. The result of this study indicated that such construction can be useful to investigate as candidates for development of detection methods for simultaneous diagnosis of both infections. Specific IgY against the recombinant VP8-S2 could be recommended as a candidate for passive immunization against bovine rotavirus and bovine coronavirus.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Performance of Environmental T-shape Noise Barriers Covered with Primitive Root Diffusers
    Autorzy:
    Monazzam, M. R.
    Naderzadeh, M.
    Nassiri, P.
    Fard, S. M. B.
    Powiązania:
    https://bibliotekanauki.pl/articles/177980.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    noise barrier
    resistive layers
    T-shape barrier
    primitive root diffuser
    Opis:
    There is a considerable increase in the use of noise barriers in recent years. Noise barriers as a control noise solution can increase the insertion loss to protect receivers. This paper presents the results of an investigation about the acoustic efficiency of primitive root sequence diffuser (PRD) on an environmental single T-shape barrier design. A 2D boundary element method (BEM) is used to predict the insertion loss of the tested barriers. The results of rigid and with a different sequence diffuser coverage are also predicted for comparison. Employing PRD on the top surface of T-shape barrier has been found to improve the performance of barriers in comparison with the use of rigid and QRD coverage at the examined receiver locations. It has been found that decreasing the design frequency of PRD shifts the frequency effects towards lower frequencies, and therefore the overall A-weighted insertion loss is improved. It was also found that using wire mesh with reasonably efficient resistivity on the top surface of PRD improves the efficiency of the reactive barriers; however utilizing wire meshes with flow resistivity higher than the specific acoustic impedance of air on the PRD top of a diffuser barrier significantly reduces the performance of the barrier within the frequency bandwidth of the diffuser. The performance of a PRD covered T-shape barrier at 200 Hz was found to be higher than that of its equivalent QRD barriers in both the far field and in areas close to the ground. The amount of improvement compared made by PRD barrier compared with its equivalent rigid barrier at far field is about 2 to 3 dB, while this improvement relative to the barrier model "QR4”"can reach up to 4–6 dB.
    Źródło:
    Archives of Acoustics; 2010, 35, 4; 565-578
    0137-5075
    Pojawia się w:
    Archives of Acoustics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Physical and mechanical properties of hemp seed
    Autorzy:
    Taheri-Garavand, A.
    Nassiri, A.
    Gharibzahedi, S.M.T.
    Powiązania:
    https://bibliotekanauki.pl/articles/24904.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Instytut Agrofizyki PAN
    Opis:
    The current study was conducted to investigate the effect of moisture content on the post-harvest physical and mechanical properties of hemp seed in the range of 5.39 to 27.12% d.b. Results showed that the effect of moisture content on the most physical properties of the grain was significant (P<0.05). The results of mechanical tests demonstrated that the effect of loading rate on the mechanical properties of hemp seed was not significant. However, the moisture content effect on rupture force and energy was significant (P<0.01).The lowest value of rupture force was obtained at the highest loading rate (3mm min-1)and in the moisture content of 27.12% d.b. Moreover, the interaction effects of loading rate and moisture content on the rupture force and energy of hemp seed were significant (P<0.05).
    Źródło:
    International Agrophysics; 2012, 26, 2
    0236-8722
    Pojawia się w:
    International Agrophysics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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