- Tytuł:
- The structure of the oligosaccharides of α3β1 integrin from human ureter epithelium (HCV29) cell line.
- Autorzy:
-
Lityńska, Anna
Pocheć, Ewa
Hoja-Łukowicz, Dorota
Kremser, Elżbieta
Laidler, Piotr
Amoresano, Angela
Monti, Chiara - Powiązania:
- https://bibliotekanauki.pl/articles/1043787.pdf
- Data publikacji:
- 2002
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
glycosylation
α3β1 integrin
cell line
MALDI MS - Opis:
- There is a growing line of evidence that glycosylation of α and β subunits is important for the function of integrins. Integrin α3β1, from human ureter epithelium cell - line HCV29, was isolated by affinity chromatography on laminin GD6 peptide. Characterization of its carbohydrate moieties was carried out using sodium dodecyl sulfate/polyacrylamide gel electrophoresis followed by Western blotting on Immobilon P and on-blot deglycosylation with peptide N-glycosidase-F. Profiles of N-glycans for each subunit were obtained by matrix-assisted laser desorption/ionization mass spectrometry. Our findings demonstrated, in both subunits of integrin α3β1, the presence of complex type oligosaccharides with a wide heterogeneity. Bi- tri- and tetraantennary structures were the most common, while high-mannose type structures were minor. Also the presence of short poly-N-acetyllactosamine entities was shown. These results show that while the predominant oligosaccharides of both subunits are identical, some slight differences between them do exist.
- Źródło:
-
Acta Biochimica Polonica; 2002, 49, 2; 491-500
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł