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Wyszukujesz frazę "Mistrzak, P." wg kryterium: Autor


Wyświetlanie 1-2 z 2
Tytuł:
A new and fast method to obtain in vitro cultures of Huperzia selago (Huperziaceae) sporophytes, a club moss which is a source of huperzine A
Autorzy:
Szypula, W.J.
Mistrzak, P.
Olszowska, O.
Powiązania:
https://bibliotekanauki.pl/articles/58228.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
clubmoss
ground pine zob.clubmoss
club moss zob.clubmoss
in vitro culture
Huperzia selago
Lycopodium selago
Huperziaceae
sporophyte
huperzine A
sesquiterpene alkaloid
pharmaceutical effect
treatment
Opis:
This study presents a protocol for a fast and effective in vitro axenic culture of Huperzia selago (Huperziaceae Rothm.) sporophytes, a club moss which is a source of huperzine A, an alkaloid of a considerable therapeutic potential extensively investigated for its uses as treatment for some neurodegenerative diseases. The proposed procedure allowed approximately tenfold shortening of the species developmental stages with the omission of the gametophyte stage while the sporophyte mass could be increased tenfold within a 6-month period. The cultures were established using vegetative propagules (bulbils) procured from sporophytes growing in the wild without degrading the habitats of this endangered plant species. Explants underwent surface and internal disinfection to eliminate the epiphytic and endophytic bacteria and fungi. In in vitro cultures, the optimum results were achieved using Moore (Mr) medium without growth regulators or supplemented with 0.015 mg/l IBA and 0.3 mg/l kinetin. These media ensured both viability of the propagules and their further development. The biomass growth index for H. selago sporophytes grown from propagules, determined at 3 months of culture (1 passage) on Mr medium with IBA and kinetin was 650%. At 6 months, the biomass growth index increased to 1114%. Vigorous growth of adventitious roots, especially on Mr medium with the addition of 0.25 mg/l NAA, and callus formation on shoot apices were observed. At 6 months of culture, some sporophytes obtained from the bulbils were used as the initiating material for shoot subcultures, which developed best on Mr medium with IBA and kinetin.
Źródło:
Acta Societatis Botanicorum Poloniae; 2013, 82, 4
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification and quantitative determination of pinoresinol in Taxus x media Rehder needles, cell suspension and shoot cultures
Autorzy:
Mistrzak, P.
Celejewska-Marciniak, H.
Szypula, W.J.
Olszowska, O.
Kiss, A.K.
Powiązania:
https://bibliotekanauki.pl/articles/56453.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
identification
quantitative determination
pinoresinol
Taxus x media
needle
cell suspension
shoot culture
Opis:
The aim of our study was to investigate the presence and quantitative contents of lignans in the tissues of Taxus ×media. The presence of the lignans: pinoresinol, matairesinol and secoisolariciresinol was assessed in needles, shoots cultures and suspension culture. Pinoresinol was the only lignan found in the tissue of T. ×media. The total pinoresinol content in the needles and in the shoots was 1.24 mg/g dry weight (dw) and 0.69 mg/g dw, respectively. Most of the pinoresinol identified was appeared glycosidically bound. In needles, the amount of glycosidically bound pinoresinol (0.81 mg/g dw) was about twice as high as that of free pinoresinol (0.43 mg/g dw). The content of free and glycosidically bound pinoresinol showed the level of 0.18 mg/g dw and 0.51 mg/g dw, respectively in the in vitro shoot cultures. In the cell culture, no pinoresinol was found.
Źródło:
Acta Societatis Botanicorum Poloniae; 2015, 84, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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