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    Tytuł:
    Evaluation of methods for Erwinia amylovora detection
    Autorzy:
    Kaluzna, M.
    Pulawska, J.
    Mikicinski, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/1986.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Ogrodnictwa
    Tematy:
    Erwinia amylovora
    fire blight
    real-time polymerase chain reaction
    LAMP technique
    DNA sequence
    detection method
    disease control
    Źródło:
    Journal of Horticultural Research; 2013, 21, 2
    2300-5009
    Pojawia się w:
    Journal of Horticultural Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Efficacy of fungicides and essential oils against bacterial diseases of fruit trees
    Autorzy:
    Mikicinski, A.
    Sobiczewski, P.
    Berczynski, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/66389.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    fungicide
    essential oil
    bacterial disease
    plant disease
    fruit tree
    Opis:
    In the framework of the performed studies, the antibacterial activity of the following fungicides was evaluated: Miedzian 50 WG (active substance - a.s. 50% copper oxychloride), Ridomil MZ Gold 68 WG (a.s. 3.8% metalaxyl-M and 64%, mancozeb), Euparen Multi 50 WG (a.s. 50% tolylfluanid), Captan 80 WG [a.s. 80% N-(captan)], Dithane Neotec 75 WG (a.s. 75% mancozeb). The evaluation also concerned the essential oils: lavender, sage, lemon balm, clove, and a preparation based on thyme oil (BioZell). Each preparation and compound was tested against the following bacterial pathogens: Erwinia amylovora, Xanthomonas arboricola pv. corylina, X. arboricola pv. juglandis, Pseudomonas syringae pv. syringae, Agrobacterium tumefaciens (presently Rhizobium radiobacter). Each preparation and compound was tested at a concentration of 1,000 ppm of active substance. Copper oxychloride was also tested at a concentration of 1,500 ppm. Among the tested fungicides, metalaxyl-M with mancozeb, mancozeb alone, and copper oxychloride inhibited all of the tested strains of pathogenic bacteria. Tolylfluanid did not inhibit any of the bacteria used. Out of the investigated essential oils, the strongest inhibitors of bacteria were: sage, cloves, and BioZell. The protective activity of the above mentioned fungicides was also evaluated in vivo. They were assessed against fire blight on apple blossoms and pear fruitlets, against bacterial canker on sweet cherry fruitlets, and against crown gall on sunflower seedlings (the test plant). All fungicides were applied at the same concentrations as those in the in vitro tests. Only copper oxychloride was found to show protective activity against the studied diseases. This result indicates that the antibacterial properties of the other fungicides did not correspond with their activity on the plant organs used in the in vivo experiment.
    Źródło:
    Journal of Plant Protection Research; 2012, 52, 4
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection, isolation, and preliminary characterization of bacteria contaminating plant tissue cultures
    Izolacja, wstępna charakterystyka i wykrywanie bakterii zanieczyszczających kultury roślinne in vitro
    Autorzy:
    Kaluzna, M.
    Mikicinski, A.
    Sobiczewski, P.
    Zawadzka, M.
    Zenkteler, E.
    Orlikowska, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/27869.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    In order to limit the contamination problem in plant tissue cultures experiments on selection of media suitable for detection and isolation of bacteria contaminating plant tissue explants, and preliminary characterization of isolates were made. In the first experiment aiming at detection of bacteria in plant explants four strains representing genera most often occurring at our survey of plant tissue cultures, and earlier isolated and identified (Bacillus, Methylobacterium, Pseudomonas and Xanthomonas) were streaked on five bacteriological media (NA, King B, K, R2A and 523) and on the medium used for plant culture initiation – 1 MS with milk albumin (IM). All strains grew on all media but on K and IM at the slowest rate and on 523 medium at the fastest. The IM medium proved to be useful for immediate bacteria detection at the initial stage of culture. In the second experiment, aiming at characterization of isolates on the basis of colony growth and morphology 14 strains (Agrobacterium, Bacillus, Curtobacterium, Flavobacterium, Lactobacillus, Methylobacterium – 2 strains Mycobacterium, Paenibacillus, Plantibacterium, Pseudomonas, Stenotrophomonas, Xanthomonas, and species Serratia marcescens) were streaked on five microbiological media: KB, NBY, YDC, YNA and YPGA. All strains grew on all those media but at different rates. The only exception was the strain of Lactobacillus spp., which did not grow on King B medium. This medium allowed the detection of such characteristic traits as fluorescence (Pseudomonas) and secretion of inclusions (Stenotrophomonas). The third experiment was focussed on assessment of the sensitivity of detection of specific bacteria in pure cultures and in plant tissue cultures using standard PCR and BIO-PCR techniques with genus specific primers and 2 methods of DNA isolation. Results showed that the use of Genomic Mini kit enabled an increase of the sensitivity by 100 times as compared to extraction of DNA by boiling. Moreover, the application of BIO-PCR increased sensitivity of detection from 102 to 105 times over the standard PCR. If looking for unknown cultivable bacteria more effective detection seems to be use of microbiological method enabling detection on bacteriological media single cells in the fragments of explants or in wash liquids, in which fragmented explants were shaken.
    W pracy określono możliwość wykrywania obecności bakterii najczęściej spotykanych w kulturach roślinnych in vitro, należących do różnych rodzajów, przy pomocy technik mikrobiologicznej i molekularnej. Za najbardziej przydatną do izolacji uznano pożywkę 523 a także pożywkę zawierającą 1 soli mineralnych MS stosowaną do inicjacji kultur roślinnych, z dodatkiem 0,025% albuminy mlecznej. Większą czułość wykrywania bakterii przy pomocy markerowego DNA uzyskano przy zastosowaniu techniki polegającej na preinkubacji badanego materiału na pożywkach bakteriologicznych, a następnie na izolacji DNA ze wzrostu (BIO-PCR). Przy porównaniu sposobów izolacji DNA większą czułość uzyskano stosując zestaw Genomic Mini (A&A Biotechnology) niż po zastosowaniu ekstrakcji w temperaturze wrzenia.
    Źródło:
    Acta Agrobotanica; 2013, 66, 4
    0065-0951
    2300-357X
    Pojawia się w:
    Acta Agrobotanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Struktura zbiorowisk grzybów i bakterii w glebie 1-rocznej uprawy i 10-letniego młodnika w zależności od sposobu przygotowania gleby
    Structure of fungal and bacterial communities in 1-year and 10-year-old plantations of Scots pine after different pre-planting preparation of soil
    Autorzy:
    Kwaśna, H.
    Łakomy, P.
    Gornowicz, R.
    Mikiciński, A.
    Borowczyk-Behnke, J.
    Gałązka, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/989686.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    uprawy lesne
    uprawy sosnowe
    mlodniki sosnowe
    sosna zwyczajna
    Pinus sylvestris
    przygotowanie gleby
    gleby lesne
    aktywnosc biologiczna
    grzyby glebowe
    bakterie glebowe
    wystepowanie
    zbiorowiska bakterii
    zbiorowiska grzybow
    struktura zbiorowisk
    armillaria
    heterobasidion
    scots pine
    silvicultural techniques
    soil biological activity
    Opis:
    Effects of post−harvest wood−debris utilization and pre−planting soil preparation in clear−cut forest on the community structure of soil fungi and bacteria and their possible biological activity towards Armillaria and Heterobasidion were studied in 1− and 10−year−old Scots pine plantations in Bierzwnik and Międzychód Forest Districts (W Poland). Post−harvest wood−debris utilization included: (i) removal from the surface, (ii) spread of the coarse or chipped wood−debris on the surface and (iii) mixing of the chipped wood debris with the soil. Pre−planting soil preparation included: (i) deep furrowing, (ii) shallow turning of the topsoil, (iii) ridging and (iv) no ground preparation. The soil−dilution method was used for detection of fungi and bacteria in soil. Morphotyping was used for identification of fungi. Phenotypic traits and biochemical properties were used for identification of bacteria. Molecular method, MID−66 or BIOLOG® systems were additionally applied for identification of the most common bacteria. Removal of post−harvest wood−debris from the surface of the clear−cut land and shallow turning of the topsoil or ridging before planting increased abundance of fungi in soil of 1−year−old Scots pine plantation. Deep furrowing resulted in increased abundance of fungi and no ground preparation in increased abundance of bacteria in soil of 10−year−old Scots pine plantation. Increased abundance of fungi and bacteria was associated with increased abundance of taxa considered as antagonistic to Armillaria and Heterobasidion. Removal of the post−harvest wood debris and moderate or no mechanical intervention into the soil habitat on the clear−cut site before planting of Scots pine seedlings seems to create the habitat, which may be beneficial for the growth of young trees.
    Źródło:
    Sylwan; 2015, 159, 01; 71-81
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of silvicultural techniques on the diversity of microorganisms in forest soil and their possible participation in biological control of Armillaria and Heterobasidion
    Autorzy:
    Kwasna, H.
    Walkowiak, L.
    Lakomy, P.
    Behnke-Borowczyk, J.
    Gornowicz, R.
    Mikicinski, A.
    Galazka, S.
    Szewczyk, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/65397.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    silvicultural technique
    Pinus sylvestris
    diversity
    microorganism
    forest soil
    participation
    biological control
    Armillaria
    fungi
    Heterobasidion
    Opis:
    Effects of different pre-planting soil preparations and post-harvest wood debris applications in a clear-cut Scots pine plantation, on the abundance, diversity, and activity of culturable microorganisms were investigated. The investigation was done 9 years after the re-plantings had been done. This formed part of an investigation of silvicultural practices for conservation and the biological control of Armillaria and Heterobasidion in northern temperate forests (Poland). The treatments being compared, were expected to have altered the soil’s physical and chemical properties, and consequently, its biological properties. Only soft-rot microfungi from the Ascomycota and Zygomycota were detected in the soil. Fungi, including those antagonistic to Armillaria and Heterobasidion, were more abundant after shallow ploughing than after deep ploughing or ridging, and where chipped rather than coarse wood debris was left on the soil surface or incorporated. Scots pine trees had the most biomass and the least mortality after ridging and leaving coarse wood debris on the surface (associated with only a relatively moderate abundance of fungi).
    Źródło:
    Journal of Plant Protection Research; 2015, 55, 3
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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