Autor: Międzobrodzki, J. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Characteristics of advanced methods used for typing bacterial isolates from mastitis with particular reference to Staphylococci
Autorzy:: Lisowska-Łysiak, K.
Dudko, P.
Kosecka-Strojek, M.
Walczak, J.
Wójcik, P.
Międzobrodzki, J.
Powiązania:: https://bibliotekanauki.pl/articles/2087754.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: coagulase-negative staphylococci
genetic methods
mastitis
phenotypic methods
Staphylococcus aureus
Źródło:: Polish Journal of Veterinary Sciences; 2018, 21, 1; 229-239
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Clustering of Staphylococcus aureus bovine mastitis strains from regions of Central-Eastern Poland based on their biochemical and genetic characteristics
Autorzy:: Puacz, E.
Ilczyszyn, W.M.
Kosecka, M.
Buda, A.
Dudziak, W.
Polakowska, K.
Panz, T.
Bialecka, A.
Kasprowicz, A.
Lisowski, A.
Krukowski, H.
Cuteri, V.
Miedzobrodzki, J.
Powiązania:: https://bibliotekanauki.pl/articles/31348.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: Staphylococcus aureus strains were isolated from mastitic milk of cows with infected mammary glands. The animals were living in 12 different farms near Lublin, in Central-Eastern Poland. A biochemical identification method based on enzymatic assay was performed, followed by haemolytic and proteolytic tests. PCR-RFLP targeted on the gap gene allowed the genetic identification of strains at the species level and verified phenotypic identification results. A molecular typing method using triplex PCR was performed to recognize the genetic similarity of the analyzed strains. DNA microar-ray hybridization (StaphyType, Alere Technologies) was used for detection of antibiotic resistance and virulence associated markers. The results obtained indicate high genetic similarity in strains isolated from the same sites. High genetic similarities were also detected between strains isolated from cows from different farms of the same region. A slightly lower similarity was noted however, in strains from various regions indicating that the strains are herd specific and that the cow's infections caused by S. aureus were of a clonal character. In 21 representative isolates selected for DNA-microarray testing, only fosfomycin (fosB) and penicillin resistance markers (blaZ, blaI, blaR) were detected. The presence of genes coding for haemolysins (lukF, lukS, hlgA, hla, hld, hlb), proteases (aur, sspA, sspB, sspP), enterotoxins (entA, entD, entG, entI, entJ, entM, entN, entO, entR entU, egc-cluster), adhesins (icaA, icaC, icaD, bbp, clfA, clfB, fib, fnbA, map, vwb) or immune evasion proteins (scn, chp, sak) was common and, with exceptions, matched triplex PCR-defined clusters.
Źródło:: Polish Journal of Veterinary Sciences; 2015, 18, 2
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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