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    Wyświetlanie 1-5 z 5
    Tytuł:
    Cloning and characterization of a Schistosoma mansoni 1H and 30S clones as two tegumental vaccine candidate antigens
    Autorzy:
    Amr, Shabaan
    Magdy, Mohamed
    Mohga, Abdallah
    Hayat, Ibrahim
    Amr, Karim
    Powiązania:
    https://bibliotekanauki.pl/articles/1043676.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    anti-GST
    Tegumental antigen
    vaccine candidate antigen
    Sm13
    Opis:
    Two Schistosoma mansoni cDNA clones 30S and 1H were identified by immunoscreening of sporocyst λgt11 library and by random sequencing of clones from λZap libraries, respectively. Clone 30S was one of 30 clones identified by an antibody raised against tegument of 3-h schistosomules. The clone was found to encode an 81 amino-acid protein fragment. It was expressed in Escherichia coli as a fusion protein of calculated molecular mass of about 35 kDa with C-terminus of Schistosoma japonicum glutathione-S-transferase (Sj26; about 26 kDa). The recombinant fusion protein was specifically recognized by serum of rabbits immunized with irradiated cercariae. Clone 1H is one of 76 expressed sequence tags derived from an adult worm library. It encodes the complete sequence of a tegumental membrane protein, Sm13. The 104 amino-acid open reading frame encodes a protein with a calculated molecular mass of about 11.9 kDa. Clone 1H was expressed in E. coli as an insoluble fusion protein with Sj26 of about 40 kDa. In Western blots, the fusion protein was recognized by serum from rabbits vaccinated with irradiated cercariae but not by preimmune rabbit sera. The cloning, characterization and expression of those proteins are therefore potentially usefull for vaccine development.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 1; 269-278
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis of Schistosoma mansoni genes using the expressed sequence Tag approach.
    Autorzy:
    Amr, Shabaan
    Magdy, Mohamed
    Mohga, Abdallah
    Hayat, Ibrahim
    Amr, Karim
    Powiązania:
    https://bibliotekanauki.pl/articles/1043675.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    BLASTX
    database analysis
    dbEST
    BLASTN
    ESTs
    Opis:
    Expressed sequence tags (ESTs) are partial cDNA sequences read from both ends of random expressed gene fragments used for discovering new genes. DNA libraries from four different developmental stages of Schistosoma mansoni used in this study generated 141 ESTs representing about 2.5% of S. mansoni sequences in dbEST. Sequencing was done by the dideoxy chain termination method. The sequences were submitted to GenBank for homology searching in nonredundant databases using Basic Local Alignment Search Tool for DNA (BLASTN) alignment and for protein (BLASTX) alignment at the National Center for Biotechnology Information (NCBI). Among submitted ESTs, 29 were derived from λgt11 sporocyst library, 70 from λZap adult worm library, 31 from λZap cercarial library, and 11 from λZap female B worm library. Homology search revealed that eight (5.6%) ESTs shared homology to previously identified S. mansoni genes in dbEST, 15 (10.6%) are homologous to known genes in other organisms, 116 (81.7%) showed no significant sequence homology in the databases, and the remaining sequences (2.1%) showed low homologies to rRNA or mitochondrial DNA sequences. Thus, among the 141 ESTs studied, 116 sequences are derived from noval, uncharactarized S. mansoni genes. Those 116 ESTs are important for identification of coding regions in the sequences, helping in mapping of schistosome genome, and identifying genes of immunological and pharmacological significance.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 1; 259-268
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Controlling the plasma electron number density of copper metal using NIR picosecond laser-induced plasma spectroscopy
    Autorzy:
    Fikry, Mohamed
    Tawfik, Walid
    Omar, Magdy
    Powiązania:
    https://bibliotekanauki.pl/articles/2033963.pdf
    Data publikacji:
    2021
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    picosecond
    LIPS
    laser induced plasma spectroscopy
    copper
    electron density
    Stark broadening
    Opis:
    In this paper, we investigate a new method to control the plasma electron number density of copper metal using a near-infrared (NIR) picosecond Nd:YAG laser-induced plasma spectroscopy (LIPS) technique. The applied laser parameters are as follows; laser pulse energy and intensity varied from 29.2 to 59.4 mJ ± 3% and from 6.01×1010 to 12.35×1010 W/cm2 ± 5%, respectively, for a single pulse at 170 ps pulse duration, and beam diameter about 0.5 ± 0.1 mm. By considering the Stark broadening of a specific spectral line, electron density can be calculated using a neutral copper line at 521.8 nm, assuming the local thermodynamic equilibrium (LTE) condition. The observed electron density values were 1.09×1016, 2.24×1016, 3.60×1016, and 4.75×1016 cm–3 for the laser pulse energies 29.2, 41, 52.4, and 59.4 mJ, respectively. The plasma electron density values are increased with the increase in laser pulse energy. Such findings were interpreted due to an increase in the mass ablation rates with laser pulse energy. The obtained results explore the ability to control the plasma electron density by controlling the picosecond pulse energy. These results can contribute to the development of plasma technologies and their applications in many fields.
    Źródło:
    Optica Applicata; 2021, 51, 3; 365-374
    0078-5466
    1899-7015
    Pojawia się w:
    Optica Applicata
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Integrated microfacies, macrobenthos and trace-fossil analysis of the lower‒middle Miocene succession of Gebel Gharra, Cairo-Suez District, Egypt
    Autorzy:
    Mandor, Mona
    El Hedeny, Magdy
    El-Sabbagh, Ahmed
    Nagm, Emad
    Abdelaal, Abdelaal
    Rashwan, Mohamed
    Mansour, Heba
    Powiązania:
    https://bibliotekanauki.pl/articles/27311287.pdf
    Data publikacji:
    2023
    Wydawca:
    Polskie Towarzystwo Geologiczne
    Tematy:
    Macrobenthos
    taphonomy
    trace fossils
    palaeoecology
    palaeoenvironment
    lower Miocene
    middle Miocene
    Egypt
    Opis:
    The palaeoecology and palaeoenvironments of the lower–middle Miocene succession in Gebel Gharra, NW of Suez, Egypt, are interpreted, on the basis of a detailed study of microfacies, trace fossils, and macrofaunal benthic assemblages. This succession consists of a lower siliciclastic part (the Gharra Formation) and a carbonate-dominated upper part (the Geniefa Formation), corresponding to a general transgression-regression cycle. Facies characteristics indicate depositional palaeoenvironments, ranging from supratidal, lagoonal, to shoal settings on an inner ramp that was influenced proximally by clastic input. At least seven ichnotaxa were recorded in the lower Miocene Gharra Formation, representing suites of the Skolithos ichnofacies. Among them, the ichnogenus Polykladichnus is recorded for the first time in Egypt. The macrobenthic taxa identified in 13 statistical samples are grouped into five assemblages (A–E) that are described and interpreted as the remains of communities. The faunal distribution and trophic structure of most of these assemblages confirm the existence of relatively stable and low-stress conditions. However, the trophic structure of the assemblages reflects the influence of particular environmental parameters, the dissolution of aragonitic shells and/or sample-size effects. Environmental parameters, controlling the distribution of trace and/or body fossils, include substrate consistency, bathymetry, water energy, productivity level, rate of sedimentation, salinity, and oxygen availability. The results of integrated lithoand biofacies analysis confirm that the succession studied was deposited in different environmental settings, providing perfect conditions for the occurrence and preservation of trace-fossil and macrobenthos assemblages.
    Źródło:
    Annales Societatis Geologorum Poloniae; 2023, 93, 4; 381--409
    0208-9068
    Pojawia się w:
    Annales Societatis Geologorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Basic Principles and Mechanical Considerations of Satellites: A Short Review
    Autorzy:
    Reda, Reham
    Ahmed, Yasmeen
    Magdy, Islam
    Nabil, Hossam
    Khamis, Mennatullah
    Refaey, Ahmed
    Eldabaa, Nada
    Abo Elmagd, Manar
    Abo Lila, Mohamed
    Ergawy, Hady
    Elgarf, Alhussein
    Abed, Gerges
    Powiązania:
    https://bibliotekanauki.pl/articles/36827035.pdf
    Data publikacji:
    2023
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Lotnictwa
    Tematy:
    principles of satellites
    primary structure
    isogrid structure
    honeycomb structure
    mechanical load analysis
    assembly
    integration
    testing
    Opis:
    Satellites are used for navigation, communication, oceanography, astronomy, etc.. Satellites come in a diversity of sizes and forms. Depending on the satellite’s mission, different subsystems are used. These subsystems are installed inside a housing to protect them from the space environment. This housing, which is also known as the satellite primary structure or mechanical structure, is made of durable materials that can endure severe conditions during launch and in the orbit. The optimisation of satellite mass is crucial right now since satellites are losing mass every day to reduce the cost of manufacturing and launching. This review first introduces an overview of the satellite classifications and subsystems. Then, the different types of mechanical load analysis the satellite subjects itself to are demonstrated. The advanced approaches for promoting the performance of the mechanical structures of satellites are explored, with a spotlight on the effect of the optimisation parameters of isogrid and honeycomb sandwich structures on the mechanical performance of the satellite primary structure. The assembly, integration and testing (AIT) of the small satellite are briefly presented. Finally, the important potential designs to improve the mechanical performance of the satellite primary structure and the challenges of further research are summarised.
    Źródło:
    Transactions on Aerospace Research; 2023, 3 (272); 40-54
    0509-6669
    2545-2835
    Pojawia się w:
    Transactions on Aerospace Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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