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Wyświetlanie 1-10 z 10
Tytuł:
Fluorometric assay of oleate-activated phospholipase D isoenzyme in membranes of rat nervous tissue and human platelets
Autorzy:
Krzystanek, Marek
Trzeciak, Henryk
Krzystanek, Ewa
Małecki, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1040386.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
human platelets
membranes
rat brain
fluorometric assay
phospholipase D
astrocytes
Opis:
Phospholipase D plays a key role in the biosynthesis of phosphatidic acid, a second messenger involved in essential cellular processes. Oleate-activated phospholipase D was the first mammalian phospholipase D isoform to be discovered but is the least known. The study was aimed to test a fluorometric method of assessment of oleate-activated phospholipase D activity in different biological materials. The brain cortex of male Wistar rats, cultured rat brain astrocytes, and human platelets were processed to yield plasmatic membranes for experiments. To assess phospholipase D activity the modified fluorometric method was used. Previously, the method was used only to determine H2O2. In this enzyme-coupled assay phospholipase D activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine. First, phospholipase D cleaves exogenous phosphatidylcholine to yield choline and phosphatidic acid. Second, choline is oxidized by choline oxidase to betaine and H2O2. Finally, in the presence of horseradish peroxidase, H2O2 reacts with 10-acetyl-3,7-dihydroxyphenoxazine to generate the highly fluorescent product, resorufin. The concentration of resorufin was measured using excitation and emission at 560 nm and 590 nm, respectively. The proposed optimal parameters of the tested assay are 25 µg of rat brain cortex protein, 50 µg of rat brain astrocyte protein, and 50 µg of human platelet protein in a reaction volume of 200 µL, and 2 min enzymatic reaction at 37°C. The fluorometric method may be applied to assay phospholipase D in different biological materials.
Źródło:
Acta Biochimica Polonica; 2010, 57, 3; 369-372
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Long-term trends and factors influencing rainwater chemistry in the Tatra Mountains, Poland
Autorzy:
Małecki, Jerzy J.
Matyjasik, Marek
Krogulec, Ewa
Porowska, Dorota
Powiązania:
https://bibliotekanauki.pl/articles/2055855.pdf
Data publikacji:
2022
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Wydawnictwo AGH
Tematy:
temporal trends
rainwater chemistry
atmospheric pollution
Tatra Mountains
Opis:
The results of rainwater chemistry monitoring in the Tatra Mountains, Poland, during the periods 1993–1994 and 2002–2019 were used to determine long-term trends and the factors influencing rainwater chemistry in the last two decades. In the early 1990’s, the study area was characterized by prominent acid rains with a pH of 4.4 that affected surface water, meadows, and forest ecosystems. A rising pH temporal trend has been observed during the following years, indicating improving air quality. This trend has also been observed in measured ionic concentrations and reduced wet deposition loads of sulfur- and nitrogen-containing acid-forming compounds. The neutralization capacity of rainwater in Kasprowy Wierch increased over the last twenty years and has mostly been dominated by NH4 + . The ammonium availability index has been steadily increasing between years 2002 and 2019 but remains less than 1. This statistically significant relationship also indicates that a portion of neutralization occurs in the lower part of the atmosphere due to ammonium-related neutralization processes. The acidic potential (AP) and the ratio AP/NP (acidic potential/neutralization potential) have been declining during the same time. The stated trends in rainwater chemistry reflect the transformation to more environmentally sustainable economies in the region. Similar changes have been observed in neighboring countries in the region, including Slovakia, the Czech Republic, and Lithuania.
Źródło:
Geology, Geophysics and Environment; 2022, 48, 1; 19--38
2299-8004
2353-0790
Pojawia się w:
Geology, Geophysics and Environment
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wkład Ludowego Wojska Polskiego w rozwój społeczno-gospodarczy i naukowo-techniczny PRL 1944-1973 : bibliografia
Współwytwórcy:
Czemarmazowicz, Elżbieta. Opracowanie
Malecki, Jerzy (bibliografia). Opracowanie
Sokołowski, Marek. Opracowanie
Dąbrowski, Maciej. Recenzja
Dębowski, D.. Recenzja
Data publikacji:
1974
Wydawca:
Warszawa : CBW
Tematy:
Wojsko Polskie ludowe bibliografia 1944-1973 r.
Opis:
Nowa bibliografia CBW.
Wkład wojska w rozwój Polski : nowa bibliografia Centralnej Biblioteki Wojskowej.
Dostawca treści:
Bibliografia CBW
Książka
    Wyświetlanie 1-10 z 10

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