- Tytuł:
- Pinellia ternata agglutinin produced in Bombyx mori cells exhibits bioactivity
- Autorzy:
-
Xu, Tao
Wang, Bo
Wang, Liya
Zhang, Yaozhou
Lv, Zhengbing - Powiązania:
- https://bibliotekanauki.pl/articles/1039738.pdf
- Data publikacji:
- 2012
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
Pinellia ternata agglutinin
silkworm bioreactor
plant lectin
Bombyx mori cells - Opis:
- Pinellia ternata agglutinin (PTA) is highly homologous to many other monocot mannose-binding lectins which reportedly possess antitumor activities. Its production in silkworm cells has great application potential because the baculovirus expression system can produce post-translationally modified proteins at low cost. In the current study, the pta gene was cloned and expressed in silkworm cells, and the expressed protein was analyzed using a hemagglutination assay. A preliminary in vitro study on its anti-proliferative activity was performed. The results show that the recombinant PTA with an apparent molecular mass of 29 kDa can hemagglutinate rabbit erythrocytes and this activity can be inhibited by D-mannan at a low concentration. In addition, the recombinant hemagglutinin exhibited a dose-dependent anti-proliferative activity on hepatoma cells. The results of the current study suggest that PTA and other important bioactive proteins could be produced by silkworm bioreactor for biomedicine research and application.
- Źródło:
-
Acta Biochimica Polonica; 2012, 59, 2; 231-236
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł