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Wyświetlanie 1-4 z 4
Tytuł:
Knitted silk mesh-like scaffold incorporated with sponge-like regenerated silk fibroin/collagen I and seeded with mesenchymal stem cells for repairing Achilles tendon in rabbits
Autorzy:
Tang, L.
Yang, Y.
Li, Y.
Yang, G.
Luo, T.
Xue, Y.
Zhang, W.
Powiązania:
https://bibliotekanauki.pl/articles/306523.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
ścięgno Achillesa
kolagen
komórki macierzyste
szpik kostny
Bombyx mori silk
regenerated silk fibroin
collagen I
bone marrow-derived mesenchymal stem cells
weft-knit
Achilles tendon action
Opis:
A scaffold knit with natural sericin-free silk fibroin fiber possesses desirable mechanical properties, biocompatibility, ease of fabrication, and slow degradability. However, regenerated silk fibroin degrades faster than natural silk. In this study, natural silk fibroin fiber mesh-like scaffolds were prepared by a weft-knitting method and the pores were filled with sponge-like regenerated silk fibroin-collagen I. The microporous sponge and mesh-like scaffolds were fused to achieve gradient degradation of the scaffolds, and rabbit bone marrow mesenchymal stem cells (BMSCs) were seeded onto the scaffolds to form scaffold–BMSCs composites. The composites were implanted into gap defects made in the rabbit Achilles tendon. Twenty weeks after implantation, histological observation showed that tendon-like tissue had formed, collagen I mRNA was expressed, abundant collagen was generated, and that there was no obvious degradation of silk. The maximum load of the neo-Achilles tendon was 62.14% that of the natural Achilles tendon. These outcomes were superior to those obtained in the group implanted with a scaffold without BMSCs. These findings suggest the feasibility of constructing tissue-engineered tendons using weft-knitted silk scaffolds incorporated with sponge-like regenerated silk fibroin/collagen I and seeded with BMSCs, and show potential of the scaffold–BMSCs composites to repair Achilles tendon defects.
Źródło:
Acta of Bioengineering and Biomechanics; 2018, 20, 3; 77-87
1509-409X
2450-6303
Pojawia się w:
Acta of Bioengineering and Biomechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental and simulative study on accumulator function in the process of wave energy conversion
Autorzy:
Zhang, W.
Liu, Y.
Luo, H.
Xue, G.
Zhang, J.
Powiązania:
https://bibliotekanauki.pl/articles/259862.pdf
Data publikacji:
2016
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
wave energy converter
AMESim simulation
accumulator
experimental verification
Opis:
In this paper, a floating-buoy wave energy converter using hydrostatic transmission system is studied. The entire work progress of wave energy power generation device is introduced, and the hydraulic transmission principles are emphasized through the simulation to verify the feasibility of design principle of hydraulic transmission system. The mathematical model of the accumulator is established and applied in the AMEsim simulation. The simulation results show that the accumulator plays an important role in the wave power hydraulic transmission system and that the correct configuration of accumulator parameters can improve the rapidity and stability of the system work. Experimental results are compared with the simulation results to validate the correctness of the simulation results. This would provide a valuable reference to the optimal design of wave power generation.
Źródło:
Polish Maritime Research; 2016, 3; 79-85
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimal design and numerical simulation on fish-like flexible hydrofoil propeller
Autorzy:
Xue, G.
Liu, Y.
Zhang, M.
Zhang, W.
Zhang, J.
Luo, H.
Jia, R.
Powiązania:
https://bibliotekanauki.pl/articles/259984.pdf
Data publikacji:
2016
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
bionics
flexible hydrofoil
phase angle
hydrodynamic coefficient
Opis:
Hydrofoil is widely used in underwater vehicle for the excellent hydrodynamic characteristics. Currently, researches are mostly about the rigid hydrofoil while the flexible hydrofoil, like the caudal fin, has not been studied adequately. In this paper, the fish was regarded as the bionic object. Then the kinematics model to describe the fish swimming was put forward. A fin-peduncle propulsion mechanism was designed based on the kinematics model to achieve the similar sine curve swimming model. The propulsion mechanism was optimized by Matlab to reduc the deviation between the output curve of the fin-peduncle propulsion mechanism and the ideal motion trajectory. Moreover, the motion phase angles among flexible articulations are optimized to reduce fluid resistance and improve propulsive efficiency. Finally, the fish-like hydrofoil oscillation is simulated by fluid-solid coupling method based on the Fluent. It was shown that the optimized flexible fish-like oscillation could generate the motion that follows the similar law of sine. The propulsive efficiency of oscillating hydrofoil propeller is much higher than that of the screw propeller, and the flexible oscillation has higher propulsive efficiency than the rigid oscillation without obvious fluid resistance increase.
Źródło:
Polish Maritime Research; 2016, 4; 59-66
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occupational exposure to 50 Hz magnetic fields does not alter responses of inflammatory genes and activation of splenic lymphocytes in mice
Autorzy:
Luo, Xue
Jia, Shujie
Li, Renyan
Gao, Peng
Zhang, Yanwen
Powiązania:
https://bibliotekanauki.pl/articles/2177064.pdf
Data publikacji:
2015-09-24
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
mice
magnetic fields
inflammation
spleen
lymphocytes
cytokine
Opis:
Objectives The objective of the present study was to observe the effects of 50 Hz magnetic fields (MFs) on the immune function of splenic lymphocytes in mice. Material and Methods Twenty male Kunming mice (6 weeks old), weighing 18– 25 g, were randomly divided into sham exposure (N = 10) and 500 μT MFs (N = 10) groups. The mice in the MFs group were exposed to 500 μT MFs for 8 h daily (5 days/week) for up to 60 days. In vitro study was carried out to examine the effects of 50 Hz MFs on the expression of inflammatory factor genes and a cluster of differentiation 69 (CD69) in mouse prime splenic lymphocytes activated by para-Methoxyamphetamine (PMA) and ionomycin. In the in vitro experiments, lymphocytes were isolated from the spleen of 10 healthy Kunming mice, the cells were cultured in the Roswell Park Memorial Institute 1640 medium (RPMI-1640) and exposed to 0 μT, 250 μT, 500 μT, or 1 mT MFs in an incubator under 5% carbon dioxide (CO₂) at 37°C for 6 h. The levels of interleukin-2 (IL-2), IL-4, interferon-gamma (IFN-γ), GATA binding protein 3 (GATA-3) and T cell-specific T-box transcription factor (T-bet) were assessed by the real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), respectively. The expression of CD69 was checked using the flow cytometry. Results Under our experimental conditions, body weight of the mice exposed to occupational, extremely low frequency- electromagnetic fields (ELF-EMFs) significantly decreased on day 20 and day 30. There were no significant changes observed in vivo in spleen weight, splenic coefficient, splenic histology profile and cytokine production in spleen tissues. Our in vitro experiments showed that 50 Hz MFs had no effect on the expression of these genes and CD69 to primary splenic cells. Conclusions In conclusion, under the applied experimental conditions, occupational exposure to 50 Hz magnetic field did not alter responses of inflammatory genes and activation of splenic lymphocytes in mice, except for body weight.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2016, 29, 2; 277-291
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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