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Wyświetlanie 1-5 z 5
Tytuł:
The amide of galacturonic acid with lysine as an immunodominant component of the lipopolysaccharide core region from Proteus penneri 42 strain
Autorzy:
Palusiak, Agata
Maciejewska, Anna
Ługowski, Czesław
Różalski, Antoni
Powiązania:
https://bibliotekanauki.pl/articles/1039351.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
amide of galacturonic acid with lysine
anti-conjugate serum
core region
lipopolysaccharide
Proteus
Opis:
Most Proteus lipopolysaccharides (LPSs) contain uronic acids or their amides with different amino acids, which together with other negatively charged components account for the acidic character of such LPS molecules. Previous studies have shown the significance of an amide of galacturonic acid with lysine [D-GalA(L-Lys)] for serological specificity of O-antigens from few P. mirabilis strains. In this work, the immunodominant role of GalALys was indicated for the P. penneri 42 LPS core region. The studies also showed the serological identity of core oligosaccharides from P. penneri 42 (O71), P. mirabilis 51/57 (O28) and R14/S1959 strains.
Źródło:
Acta Biochimica Polonica; 2014, 61, 1; 129-132
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lactoferrin-monophosphoryl lipid a complex enhances immunity of mice to Plesiomonas shigelloides CNCTC 138/92
Autorzy:
Chodaczek, Grzegorz
Zimecki, Michal
Lukasiewicz, Jolanta
Lugowski, Czesław
Powiązania:
https://bibliotekanauki.pl/articles/1040820.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
lactoferrin
Plesiomonas shigelloides
adjuvant
monophosphoryl lipid A
Opis:
Our previous study showed the efficacy of lactoferrin-monophosphoryl lipid A isolated from Hafnia alvei LPS complex (LF-MPLH.a.) as an adjuvant in stimulation of humoral and cellular immune response in mice to conventional antigens and a lower pyrogenicity of the complex as compared with MPLH.a. alone. In the present investigation we demonstrated that LF-MPLH.a. complex enhanced the immunity of BALB/c mice immunized with Plesiomonas shigelloides CNCTC 138/92 bacterial vaccine, against P. shigelloides infection. The adjuvant effect was evidenced by a significant increase of the antigen-specific serum IgG, IgG2a, and IgG1 and elevation of antigen-specific serum IgA concentrations. In addition, application of the adjuvant facilitated better clearance of the bacteria in spleens and livers of infected mice when compared with MPLH.a. alone. These features of the new adjuvant may predispose it for vaccination protocols in humans.
Źródło:
Acta Biochimica Polonica; 2008, 55, 1; 91-96
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of serological specificity of anti-endotoxin sera directed against whole bacterial cells and core oligosaccharide of Escherichia coli J5-tetanus toxoid conjugate*.
Autorzy:
Lukasiewicz, Jolanta
Jachymek, Wojciech
Niedziela, Tomasz
Malik-Gebicka, Malgorzata
Dzieciatkowska, Monika
Lugowski, Czeslaw
Powiązania:
https://bibliotekanauki.pl/articles/1043742.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
antibody
endotoxin
Escherichia coli J5
neoglycoconjugate
core region
Opis:
The rough mutants of Gram-negative bacteria are widely used to induce protective antisera but the nature of the target epitope for such antibodies is not precisely defined. Endotoxin is one of several antigens present on the surface of bacterial cells, which are able to elicit specific antibodies. We studied the specificity of antibodies produced against a conjugate of E. coli J5 endotoxin core oligosaccharide with tetanus toxoid. The use of chemically defined antigen for immunisation excludes the possibility of production of antibodies against other cell surface antigens. A comparison of this monospecific anti-endotoxin serum with antiserum against E. coli J5 whole cells was performed in order to distinguish the role that endotoxin core oligosaccharide plays in the interaction with humoral host defences from that of other potentially important Gram-negative bacterial surface antigens. The reactivity of both sera with smooth and rough lipopolysaccharides was determined in ELISA, immunoblotting and by flow cytometry. Both antisera reacted with similar specificity with most lipopolysaccharides of identical or related core type. Less distinct reactions with endotoxins of the antibacterial serum in comparison with the anti-conjugate serum were found in all serological tests. LPS of E. coli O100 that showed the strongest reactions with both sera was used to stimulate IL-6, TNFα and nitric oxide production by the J-774A.1 cell line. Both sera were used to inhibit that stimulation and no inhibitory effects of the examined sera in comparison with non-immune serum were observed.
Źródło:
Acta Biochimica Polonica; 2002, 49, 3; 721-734
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structural and serological characterization of Hafnia alvei lipopolysaccharide core region
Autorzy:
Ługowski, Czesław
Niedziela, Tomasz
Jachymek, Wojciech
Klonowska, Agnieszka
Czarny, Anna
Rowiński, Stefan
Petersson, Carl
Kenne, Lennart
Powiązania:
https://bibliotekanauki.pl/articles/1045281.pdf
Data publikacji:
1995
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1995, 42, 1; 51-54
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-5 z 5

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