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Tytuł:
Incubator for individual eggs of fish
Autorzy:
Bardega, R.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363084.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
jakość ikry
inkubacja
przeżywalność
reprodukcja ryb
egg quality
incubation
survival
fish reproduction
Opis:
We have constructed and tested a device for incubation of individual eggs of fish. We measured the diameter of fertilized and water hardened eggs of vendace (Coregonus albula). Each egg developed in a separate incubator, which was a modified Pasteur pipette. Incubation of 315 eggs resulted in 249 hatched embryos (eleutheroembryos). The technique enabled identification of each eleutheroembryo hatching from a given egg. Example of relationship between external diameter of egg and the length of eleutheroembryo showed potential applications of the technique in studies on fish reproduction biology.
Źródło:
Environmental Biotechnology; 2007, 3, 2; 49-51
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biotechnology in freshwater finfish aquaculture
Autorzy:
Luczynski, M.
Ocalewicz, K.
Powiązania:
https://bibliotekanauki.pl/articles/363230.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
akwakultura
biotechnologia
inżynieria genetyczna
pstrąg tęczowy
aquaculture
fish
biotechnology
genetic engineering
rainbow trout
Źródło:
Environmental Biotechnology; 2007, 3, 2; 37-38
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microsatellite DNA polymorphism in sturgeon species and their hybrids reared in Polish aquaculture farms
Autorzy:
Fopp-Bayat, D.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363194.pdf
Data publikacji:
2006
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
jesiotr
identyfikacja gatunkowa
DNA mikrosatelitarne
hybrydy
polimorfizm
hybrids
microsatellite DNA
polymorphism
PCR
species identification
sturgeons
Opis:
Highly variable microsatellite DNA loci show high levels of variation per locus and provide molecular markers for species and for populations of small effective size. In this study we applied microsatellite markers to identify specimens belonging to several sturgeon species and their interspecific hybrids. Nine microsatellite loci (Afu-19, Afu-22, Afu-34, Afu-39, Afu-54, Afu-57, Afu-58, Afu-68, Afu-69) were analysed for five species (beluga, Siberian sturgeon, Russian sturgeon, sterlet and paddlefish) and for their three interspecific hybrids: Siberian sturgeon x Russian sturgeon, beluga x sterlet (called bester) and for beluga x bester. Certain alleles at five loci: Afu-22, Afu-39, Afu-54, Afu-57 and Afu-68 were diagnostic for the examined sturgeon species and their interspecific hybrids.
Źródło:
Environmental Biotechnology; 2006, 2, 1; 11-19
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Gynogenesis in northern pike: UV-inactivation of spermatozoa and the heat shock inducing meiotic diploidization
Autorzy:
Luczynski, M. J.
Dabrowski, K.
Kucharczyk, D.
Glogowski, J.
Luczynski, M.
Szczerbowski, A.
Babiak, I.
Powiązania:
https://bibliotekanauki.pl/articles/363226.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
Esox lucius
gynogeneza
szok termiczny
szczupak pospolity
kontrola płci
UV
gynogenesis
heat shock
northern pike
sex control
Opis:
Gynogenetic northern pike (Esox lucius L.) were produced using UV irradiated sperm and heat shock applied to inseminated eggs shortly after gamete activation. Milt was diluted in immobilizing solution (1:9) and UV irradiated (6.4 W*m-2) for 2-20 min, with dosage in the range of 768-7680 J*m-2. Genetic inactivation of spermatozoa was most efficient when milt was irradiated for 8 min (3072 J*m-2). Insemination of eggs with irradiated milt yielded 100% haploid larvae with hatching rate at 72.1۪.8% (mean±SD), expressed as a percentage of inseminated eggs. Haploid embryo developed and most of them hatched (showing "haploid syndrome") but all haploid larvae died within 48 hours after hatching. After insemination with irradiated sperm the eggs were exposed to a thermal shock of 34°C or 34.5°C, lasting 3 or 5 min, applied 11-16 min after gamete activation. The efficiency of heat shock and survival in experimental groups significantly depended on the source (individual female effect) and quality of eggs. The time of application and duration of the heat shock also affected the survival of embryo and percentage of gynogenetic larvae. Gynogenetic larvae were obtained in all experimental groups with hatching rate as high as 24.2ۭ.6%.
Źródło:
Environmental Biotechnology; 2007, 3, 2; 39-43
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of ancient mitochondrial DNA of the Baltic Sea sturgeon (Acipenser sp.)
Autorzy:
Fopp-Bayat, D.
Ciesielski, S.
Luczyński, M.
Powiązania:
https://bibliotekanauki.pl/articles/363174.pdf
Data publikacji:
2005
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
jesiotr
historyczny mtDNA
filogeneza molekularna
gen cytochromu b
Morze Bałtyckie
Acipenser baeri
Acipenser oxyrinchus
Acipenser sturio
ancient mtDNA
cytochrome b gene
molecular phylogeny
Opis:
Genetic relatedness between Baltic Sea sturgeon (Acipenser sturio L.) specimens caught in different geographic areas is not clear. According to previous studies, fish captured in different locations within the historic area of A. sturio habitation are genetically different to each other. We have examined a fragment (191 base pairs) of mitochondrial cytochrome b gene of four specimens of A. sturio found in Poland: three fish were preserved in museums of natural history and the bone of one fish was from an archaeological site. DNA sequences of the three museum samples were identical, whereas the DNA sequence of the archaeological sample differed in the 918 position of the cytochrome b gene. All the analyzed DNA fragments were similar to those of Acipenser baeri and genetically distant to Acipenser sturio and A. oxyrinchus.
Źródło:
Environmental Biotechnology; 2005, 1, 1; 29-33
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of mercury in muscles, liver and gills of marine and freshwater fish
Autorzy:
Luczynska, J.
Luczynski, M.J.
Paszczyk, B.
Powiązania:
https://bibliotekanauki.pl/articles/1189779.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
fish
total mercury
mercury
muscle
liver
gill
marine fish
freshwater fish
body weight
total length
biotic factor
condition factor
Opis:
In this study, the total mercury concentration was determined in the muscles, liver and gills of six fish species (rainbow trout Oncorhynchus mykiss Walb., carp Cyprinus carpio L., bream Abramis brama L., perch Perca fluviatilis L., ide Leuciscus idus L. and flounder Platichthys flesus L.). The fish were acquired from October to November 2012. Mercury was analyzed by atomic absorption spectrometry using thermal decomposition, compounds of mercury and amalgamation. The mercury content in fish organs reached 0.006-0.168 (in mg kgˉ¹ wet weight) in muscles, 0.001-0.027 in gills and 0.003-0.045 in the liver. The muscles of perch and ide had significantly more Hg compared to the other fish’s muscles (P≤0.05). The liver and gills of perch, ide and flounder contained more Hg than the same tissues of the other fish (P≤0.05). Generally, the highest Hg content was determined in muscles (except rainbow trout) (P≤0.05), whereas the lowest Hg content was found in gills (except perch) (P≤0.05). The content of Hg in gills of perch did not differ from the one in the liver (P>0.05). There was a positive correlation between the weight or length of a fish and the Hg concentration in its tissues, except for the length and Hg in the gills of carp. However, a statistically significant positive correlation between the body weight and the Hg levels in fish appeared only in the case of the organs of ide (P≤0.004) and muscles of carp (P≤0.038). The correlation between the factor condition and the content of Hg, albeit positive (0.106 < r < 0.811) except for the organs of flounder, was not statistically significant (P>0.05).
Źródło:
Journal of Elementology; 2016, 21, 1; 113-129
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary evaluation of mutagenic activity of two amino derivatives of cyclopenta[c]phenanthrene
Autorzy:
Łuczyński, M. K.
Góra, M.
Mielżyńska, D.
Brzuzan, P.
Tejs, S.
Pirogowicz, P.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363278.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
test Amesa
fenantren
mutacje DNA
WWA
Ames test
phenanthrene
DNA mutations
PAHs
Opis:
The mutagenicity of two, newly synthesized amino-derivatives of cyclopenta[c]phenanthrene (CP[c]Ph) was investigated in the Ames test using TA98 and TA100 histidine dependent Salmonella typhimurium strains. Neither of the examined compounds showed mutagenic activity without metabolic activation. The incorporation of an activation system caused high mutagenicity of CP[c]Ph derivatives in both S. typhimurium strains. The results are compared with the previous data on the mutagenic activity of unsubstituted CP[c]Ph and possible mechanisms of activation are discussed.
Źródło:
Environmental Biotechnology; 2007, 3, 1; 15-19
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genotoxicity of cyclopentha[c]phenanthrene and its two derivatives based on an in vitro micronucleus test
Autorzy:
Rudnicka, K.
Łuczyński, M. K.
Góra, M.
Powiązania:
https://bibliotekanauki.pl/articles/363298.pdf
Data publikacji:
2012
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
derivatives
genotoxicity
in vitro micronucleus test
PAHs
V79
pochodne
genotoksyczność
test mikrojądrowy in vitro
Opis:
Environmental pollution causes a variety of health problems, including cancer. Many known pollutants have carcinogenic properties and polycyclic aromatic hydrocarbons (PAH) belong to this group. In this study, an in vitro culture of V79 cells of the Chinese hamster was subjected to three tested PAHs: 5-amino-2,3-dihydro-1H-cyclopenta-[c]phenanthrene (ACP[c]Ph), 5-amino-9-methoxy-2,3-dihydro-1H-cyclopenta[c]phenanthrene (AMCP[c]Ph) and cyclopenta[c]phenanthrene (CP[c]Ph). The in vitro micronucleus (MN) assay was applied in order to evaluate the genotoxic properties of the studied compounds. The highest genotoxic effect was observed for AMCP[c]Ph in a concentration of 0.02μg·ml-1. The genotoxic effect of the other two compounds was slightly lower.
Źródło:
Environmental Biotechnology; 2012, 8, 2; 63-66
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of mercury in muscles, liver and gills of marine and freshwater fish
Autorzy:
Luczynska, J.
Luczynski, M.J.
Paszczyk, B.
Powiązania:
https://bibliotekanauki.pl/articles/13699.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
fish
total mercury
mercury
muscle
liver
gill
marine fish
freshwater fish
body weight
total length
biotic factor
condition factor
Opis:
In this study, the total mercury concentration was determined in the muscles, liver and gills of six fish species (rainbow trout Oncorhynchus mykiss Walb., carp Cyprinus carpio L., bream Abramis brama L., perch Perca fluviatilis L., ide Leuciscus idus L. and flounder Platichthys flesus L.). The fish were acquired from October to November 2012. Mercury was analyzed by atomic absorption spectrometry using thermal decomposition, compounds of mercury and amalgamation. The mercury content in fish organs reached 0.006-0.168 (in mg kgˉ¹ wet weight) in muscles, 0.001-0.027 in gills and 0.003-0.045 in the liver. The muscles of perch and ide had significantly more Hg compared to the other fish’s muscles (P≤0.05). The liver and gills of perch, ide and flounder contained more Hg than the same tissues of the other fish (P≤0.05). Generally, the highest Hg content was determined in muscles (except rainbow trout) (P≤0.05), whereas the lowest Hg content was found in gills (except perch) (P≤0.05). The content of Hg in gills of perch did not differ from the one in the liver (P>0.05). There was a positive correlation between the weight or length of a fish and the Hg concentration in its tissues, except for the length and Hg in the gills of carp. However, a statistically significant positive correlation between the body weight and the Hg levels in fish appeared only in the case of the organs of ide (P≤0.004) and muscles of carp (P≤0.038). The correlation between the factor condition and the content of Hg, albeit positive (0.106 < r < 0.811) except for the organs of flounder, was not statistically significant (P>0.05).
Źródło:
Journal of Elementology; 2016, 21, 1
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
East-Siberian coregonid fishes: their occurrence, evolution and present status
Autorzy:
Yakhnenko, V. M.
Mamontov, A. M.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363220.pdf
Data publikacji:
2008
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
system wodny
zlodowacenie
aktywność tektoniczna
jezioro
pochodzenie
coregonidy
specjacja
sympatryczność
allopatry
coregonids
glaciation
origin of lakes
speciation
sympatry
tectonic activity
water systems
Opis:
Coregonid fishes (coregonids) (Salmonidae, Coregoninae) of Siberia show immense morphological and ecological variability, what is reflected in their complex interspecific and intraspecific structure: i.e. occurrence of numerous species, forms and endemic species. Studies of the coregonids of Siberia is crucially important for understanding of their origin, taxonomy and evolution. We have analysed electrophoretically some 13 enzyme systems from over 20 samples of East-Siberian coregonids. This paper was intended as an introduction to a number of primary papers that are planned that will describe the biological and genetic characteristics of studied populations.
Źródło:
Environmental Biotechnology; 2008, 4, 2; 41-53
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polymorphism of microsatellite loci - a tool in studying biodiversity of paddlefish aquaculture broodstock
Autorzy:
Kaczmarczyk, D.
Kohlmann, K.
Kersten, P.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363088.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
izolacja DNA
DNA mikrosatelitarne
amplifikacja PCR
DNA extraction
microsatellite DNA
PCR amplification
Opis:
American paddlefish (Polyodon spathula) is a new species in Polish aquaculture, its broodstocks are few and small, and it is possible that all mature fish originated from only a few spawners. Studies on polymorphism of highly variable microsatellite DNA allow revealing genetic characteristics of individual spawners as well as estimation of genetic variation within and divergence between broodstocks. This paper describes optimised protocols for isolation of DNA from fin tissues, amplification of nine microsatellite loci using PCR technique, and for fish genotyping using automatic capillary DNA sequencer. Our technique was tested towards the fin samples taken from all paddlefish reared in Poland and approaching their sexual maturity; the study included also samples taken from 47 fish of the Ukrainian breeding center (Gorny Tykich).
Źródło:
Environmental Biotechnology; 2007, 3, 2; 44-48
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Relative quantification of CYP1A gene expression in whitefish (Coregonus lavaretus) exposed to benzo[a]pyrene
Autorzy:
Brzuzan, P.
Jurczyk, Ł.
Łuczyński, M. K.
Góra, M.
Powiązania:
https://bibliotekanauki.pl/articles/363188.pdf
Data publikacji:
2005
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
ekspresja genu CYP1A
sieja
benzo(a)piren
real-time PCR
benzo(a)pyrene
Coregonus lavaretus
CYP1A gene expression
RealTime PCR
relative quantification
Opis:
The expression of CYP1A (cytochrome P4501A) can be induced by a number of aromatic compounds in teleost fishes. We developed a real-time PCR assay for measuring relative quantities (RQ) of CYP1A mRNA in whitefish (Coregonus lavaretus). To test for the usefulness of the assay we performed a treatment study, using benzo[a]pyrene (B[a]P) a model CYP1A inducer. Primers for the CYP1A gene were adapted from the literature, whereas those for [beta]-actin (endogenous control) were designed from a region that was found to be conserved among salmonid [beta]-actin genes. A group of hatchery raised whitefish, with an average body mass of 15 g and total length of 12 cm were given an intraperitoneal injection (10 mg/kg) of B[a]P in corn oil (2 mg B[a]P/ml corn oil) or corn oil alone (Control). After 48 h, whitefish liver, head kidney and brains were collected for mRNA isolation and analysis. In all three tissues sampled, CYP1A mRNA was affected by treatment with B[a]P. Head kidney tissue showed the greatest induction potential (RQ=11.00) from base levels (RQ=1.00), followed by liver (RQ=9.45), and brain (RQ=3.76). These results demonstrated that CYP1A was highly inducible by B[a]P in whitefish head kidney and liver, and to some extent, in brain tissue. The approach presented here has the advantage of providing rapid and accurate measures of CYP1A induction in various tissues of fish responding to PAH contaminant exposure.
Źródło:
Environmental Biotechnology; 2005, 1, 1; 11-15
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of by-products and waste in the synthesis of nanosilver particles
Autorzy:
Trepanowska, B.
Łuczyński, M. K.
Kulesza, S.
Adamczak, M.
Powiązania:
https://bibliotekanauki.pl/articles/363244.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
nanobiotechnology
nanoparticles
nanosilver
plant extracts
raspberry
strawberry
nanotechnologia
nanocząstki
nanosrebro
ekstrakt roślinny
malina
truskawka
Opis:
Extracts from strawberry and raspberry leaves, carrot pomace, and spent grains, were tested as bioreducing agents for the synthesis of nanosilver particles (AgNP). Based on UV vis spectra, the leaf extracts produced the most AgNP, carrot pomace was less effective, and spent grains did not produce AgNP. The dynamic light scattering method revealed that AgNP ranged from 1 to 92nm in size, and that over 80% of the particles were about 10nm. Energy dispersive X ray spectroscopy showed that elements that typically stabilize nanoparticles were present. The well diffusion method (nutrient agar medium) indicated that AgNP synthesized with raspberry leaf extract exerted strong bacteriostatic and bactericidal activity against Gram negative bacteria and weaker activity against Gram positive bacteria. Although further analysis is needed to determine the mechanism of their synthesis, the results of this study show that plant extract based synthesis can produce nanoparticles with controlled size and morphology.
Źródło:
Environmental Biotechnology; 2015, 11, 1; 14-19
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
CYP1A gene expression in adipose fin of rainbow trout (Oncorhynchus mykiss Walbaum) exposed to benzo[a]pyrene
Autorzy:
Brzuzan, P.
Woźny, M.
Łuczyński, M. K.
Góra, M.
Powiązania:
https://bibliotekanauki.pl/articles/363276.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
płetwa tłuszczowa
benzo(a)piren
CYP1A mRNA
skrzela
pstrąg tęczowy
real-time PCR
adipose fin
benzo(a)pyrene
gill
rainbow trout
Real-Time PCR
Opis:
Proximate to the environment, adipose fin of fish may be considered as a lipid storing tissue, and thus can be a target for either waterborne or dietary polycyclic aromatic compounds (PACs). We determined the effects of benzo[a]pyrene (B[a]P), a model PAC member, on CYP1A gene expression in adipose fin and compared that with the effects in gill of juvenile rainbow trout (Oncorhynchus mykiss Walbaum) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). The results of the study demonstrated that constitutive CYP1A mRNA was present in adipose fin of rainbow trout, but the transcripts were far less abundant than those in gill tissue. We confirmed high CYP1A gene induction potential of the gills in rainbow trout injected with benzo[a]pyrene, but also showed moderately and transiently induced CYP1A mRNA in adipose fin. The modest and transitory gene expression may preclude rainbow trout adipose fin CYP1A mRNA levels from using it as an indicator of sustained exposure of fish to the polycyclic aromatic compounds.
Źródło:
Environmental Biotechnology; 2007, 3, 1; 20-24
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Oxidative metabolism, mutagenic and carcinogenic properties of some polycyclic aromatic hydrocarbons
Autorzy:
Łuczyński, M. K.
Góra, M.
Brzuzan, P.
Wilamowski, J.
Kozik, B.
Powiązania:
https://bibliotekanauki.pl/articles/363172.pdf
Data publikacji:
2005
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
metabolizm tlenowy
właściwości mutagenne
właściwości rakotwórcze
wielopierścieniowe węglowodory aromatyczne
oxidative metabolism
mutagenic properties
carcinogenic properties
polycyclic aromatic hydrocarbons
Opis:
This attempt to synthesize the present state of knowledge on the correlation between the structure of chosen PAHs and their mutagenic or carcinogenic activity shows that the dependencies cannot be treated univocally. Extreme complexity of the subject, typical for investigations on the boundary between chemistry and biology, inclines one to search for new ways of synthesis of new compounds, which would be of interest for biologists studying the problem of chemical carcinogenesis. On the other hand, there is a strong need for elaborating a standardized test that would explicitly define the carcinogenic properties of a specific compound. The use of a variety of methods for the determination of carcinogenic potency of chemical compounds combined with simultaneous differentiation of structure of examined compounds, sometimes leads to contradictory conclusions. Correlation between mutagenic and carcinogenic activity of some compounds is also sometimes questionable. Modern molecular biology should allow for elaborating a method which would give a satisfactory and reliable answer to the fundamental question: is a specific compound carcinogenous for a human organism? And if so - to what extent?
Źródło:
Environmental Biotechnology; 2005, 1, 1; 16-28
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
MicroRNA expression in liver of whitefish (Coregonus lavaretus) exposed to microcystin-LR
Autorzy:
Brzuzan, P.
Woźny, M.
Wolińska, L.
Piasecka, A.
Łuczyński, M. K.
Powiązania:
https://bibliotekanauki.pl/articles/363280.pdf
Data publikacji:
2010
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
Coregonus lavaretus
Dicer
histon H2A
mikrocystyna LR
miRNAs
toksyczność
histone H2A
microcystin LR
toxicity
Opis:
MicroRNAs (miRNAs) are small, highly conserved, non-coding RNAs that regulate gene expression of target mRNAs through cleavage or translational inhibition. In the field of toxicology, the relationship between toxicity and microRNA expression is poorly understood. In the present study we analyzed the abundance of 9 selected miRNAs (omy-miR-21, omy-miR-21t, omy-miR-122, omy-miR-125a, omy-miR-125b, omy-miR-125t, omy-miR-199-5a, omy-miR-295, omy-let-7a) and mRNA of 3 genes (histone H2A, ribosome protein rpl19, and Dicer which is a miRNA processing enzyme) in liver samples of whitefish exposed to Microcystin-LR (MC-LR) at a dose of 100µg*kg-1body weight for 24 or 48h. In the examined liver tissue, omymiR-122 showed the highest relative constitutive level, what is consistent with data obtained from fish and mammals. Unexpectedly, the reference H2A mRNA level was consistently up-regulated (over 20-fold; P<0.05) in fish liver after both 24 and 48h of exposure to MC-LR. The result may suggest that MC-LR acts as an initiator of specific cell-physiologic signals triggering DNA replication in fish liver cells. MC-LR treatment had no effect on the examined miRNAs levels, except for omy-miR-125a and omy-let-7a. Whereas omy-miR-125a was up-regulated (ER=2.68; S.E. 1.61-6.78; P<0.05), omy-let-7a was down-regulated (ER=0.55; S.E. 0.32-0.79; P<0.05) in whitefish liver after 48h of the treatment with MC-LR, when compared to controls. More work with the fish is essential for understanding the crosstalk of the regulatory network controlled by the two miRNAs in the context of MC-LR toxicity.
Źródło:
Environmental Biotechnology; 2010, 6, 2; 53-60
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Benzo[a]pyrene and cyclopenta[c]phenanthrene suppress expression of p53 in head kidney of rainbow trout
Autorzy:
Brzuzan, P.
Woźny, M.
Góra, M.
Łuczyński, M. K.
Jabłońska, A.
Powiązania:
https://bibliotekanauki.pl/articles/363082.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
B[a]P
CP[c]Ph
ekspresja genów
hnRNA
pstrąg tęczowy
poziom transkrypcji
gene expression
rainbow trout
transcription rate
Opis:
Although p53, a protein of important tumor suppressive function, has been extensively studied in mammals, relatively little is known about the p53 pathways in lower vertebrates. Particularly, limited information exists on possible influences of environmental contaminants on the expression of the p53 gene in fish. In the current study, we assessed the effects of benzo[a]pyrene (B[a]P; potent tumor promoter) and cyclopenta[c]phenanthrene (CP[c]Ph; clastogenic agent) exposure on a 24h profile of p53 gene expression in head kidney of juvenile rainbow trout (Oncorhynchus mykiss). To analyze the p53 transcription rate, we developed protocol for the examination of both mRNA and heterogeneous nuclear (hn) RNA of the gene, using Real-Time RTPCR approach. The results show that both compounds are capable of suppressing p53 transcriptional activity within 12h of the treatment. Our finding supports the idea that structurally different PAHs may influence cell physiologic functions controlled by p53 in fish, in part, by down-regulating its RNA expression levels.
Źródło:
Environmental Biotechnology; 2011, 7, 1; 41-45
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary evaluation of ER- and AhR-mediated gene expression patterns in rainbow trout (Oncorhynchus mykiss) liver after short-term exposure to zearalenone in binary mixtures
Autorzy:
Woźny, M.
Brzuzan, P.
Wolińska, L.
Góra, M.
Łuczyński, M. K.
Powiązania:
https://bibliotekanauki.pl/articles/363144.pdf
Data publikacji:
2010
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
receptor AhR
benzo(a)piren
ścieżka sygnałowa
17beta-estradiol
receptor estrogenowy
zearalenone
aryl hydrocarbon receptor
benzo(a)pyrene
crosstalk
estrogen receptor
Opis:
While estrogenic properties of mycotoxin zearalenone (ZEA) has been an extensively studied issue, little is known about molecular background of its biological responses that cannot be simply explained by the estrogenic potential. The present study describes effects of ZEA (10mg*kg-1 body weight) in binary mixtures either with benzo[a]pyrene (B[a]P), or with 17β-estradiol (E2) on ER- and AhR-dependent gene expression in juvenile rainbow trout liver, evaluated using Real-Time qPCR. The study revealed dual nature of ZEA, as the treatment with this compound alone increased mRNA levels of both ER- and AhR-mediated gene expression. However, our results did not show any synergistic or additive effect of ZEA in binary mixures with E2 or B[a]P on studied gene expression levels. Whether the intriguing potential of ZEA to elicit distinct signals was a result of binding affinity to AhR or/and ER and AhR mutual receptor interactions, should be investigated in further experiments.
Źródło:
Environmental Biotechnology; 2010, 6, 1; 16-23
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of propofol and carbon dioxide on acid-base balance in Siberian sturgeon
Autorzy:
Gomulka, P.
Czerniak, E.
Dagowski, J.
Luczynski, M.
Szczerbowski, A.
Szkudlarek, M.
Powiązania:
https://bibliotekanauki.pl/articles/32060.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to compare physiological responses in Siberian sturgeon (Acipenser baeri) induced by propofol and CO2 anaesthesia. Two procedures were applied during the experiment. In procedure I, blood samples were collected immediately after exposure (1, 2, 5, 10 min) to the anaesthetic. In procedure II, fish were exposed to the anaesthetic for 10 minutes and then were moved to anaesthetic free water. Blood was sampled after 5, 10, 20 or 30 min of recovery time. Gasometrical and biochemical analyses were performed on collected blood. In CO2 anaesthetized fish strong hypercapnic acidosis was revealed. The drop of the HCO3-/CO2 ratio, from 28:1 in control fish up to 4:1 in CO2 anaesthetized ones, proved that the compensation mechanism is not capable of preventing acidosis during CO2 anaesthesia in Siberian sturgeon. In contrast, only moderate, respiratory acidosis occurred in sturgeons anaesthetised with propofol. Hypercapnic acidosis during CO2 anaesthesia was followed by a fourfold increase of ammonia level in the blood. Glucose level, increasing only during recovery time, indicates that a secondary stress response occurred when awareness of anaesthetized fish had been restored.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cyclopenta[c]phenanthrene induction of CYP1A in brain of rainbow trout (Oncorhynchus mykiss)
Autorzy:
Brzuzan, P.
Woźny, M.
Łuczyński, M. K.
Góra, M.
Ciesielski, S.
Kuźmiński, H.
Powiązania:
https://bibliotekanauki.pl/articles/363272.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
benzo(a)piren
mózg
CYP1A
cyklopenta[c]fenantren
absolutna kwantyfikacja mRNA
pstrąg tęczowy
benzo(a)pyrene
brain
cyclopenta[c]phenanthrene
mRNA absolute quantification
rainbow trout
Opis:
We assessed the effects of cyclopenta[c]phenanthrene (CP[c]Ph) and benzo[a]pyrene (B[a]P; positive control) on CYP1A gene expression in brain of juvenile rainbow trout (Oncorhynchus mykiss) using the quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). A group of hatchery raised rainbow trout, with an average body mass of 49.4 g and total length of 15.5 cm were given an intraperitoneal injection (10 mg*kg-1) of either CP[c]Ph or B[a]P in corn oil (2 mg*mi-1 corn oil) or corn oil alone (control). After 24 and 48 h, trout brains were collected for mRNA isolation and analysis. After 24 hours of the exposure, only B[a]P-treated rainbow trout had 10-fold higher number of CYP1A transcripts (mean = 3.63*106 transcripts*µg-1 total RNA) than control fish (3.24*105 transcripts*µg-1 total RNA; Tukey test, P<0.05). After 48 hrs, significantly higher levels of CYP1A expression (Tukey test, P<0.001) were found in either CP[c]Ph- or B[a]P- induced group (1.45*106 and 6.92*106 transcriptsźµg-1 total RNA, respectively) over a control group (mean=1.41*105 transcripts*µg-1 total RNA). The finding that CYP1A in brain tissue was inducible by CP[c]Ph, a polycyclic aromatic hydrocarbon (PAH) of different than B[a]P planar characteristics, may further validate the use of rainbow trout brain CYP1A mRNA levels as a biomarker of PAH exposure.
Źródło:
Environmental Biotechnology; 2007, 3, 1; 10-14
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
2,4-diphenylthiophene induces mainly base pair mutation in Salmonella Typhimurium
Autorzy:
Budzikur, K. A.
Mielżyńska-Švach, D.
Góra, M.
Chachaj, A.
Pawłowski, M.
Łuczyński, M. K.
Powiązania:
https://bibliotekanauki.pl/articles/363086.pdf
Data publikacji:
2012
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
test Amesa
związki heterocykliczne
mutagenność
siarkowe wielopierścieniowe węglowodory aromatyczne
Ames test
heterocyclic compounds
mutagenicity
sulfur-PAHs
Opis:
Heterocyclic aromatic compounds containing sulfur (S-HET), have been detected in air, soil, marine environment and freshwater sediment. Toxicity and mutagenicity data of this class of substances are scarce. The present study focuses on implications of two aryl thiophenes and their mutagenic properties in Salmonella/microsome test. In our experiment only 2,4-diphenylthiophene showed little mutagenic effect in both variants of activaction (+/-S9) in strain TA100. Thiophene ring joined to K-region of phenanthrene did not change the biological activity of 3,6-dimetoxyphenanthro [9,10-c]thiophene and this compound did not show mutagenic potency.
Źródło:
Environmental Biotechnology; 2012, 8, 1; 28-31
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mutagenicity induced in Salmonella strains TA98 and TA100 by diphenylthiophenes
Autorzy:
Budzikur, K. A.
Góra, M.
Chachaj, A.
Mielżyńska-Švach, D.
Tejs, S.
Łuczyński, M. K.
Powiązania:
https://bibliotekanauki.pl/articles/363118.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
test Amesa
mutacja
monomery tiofenowe
Ames test
mutation
thiophene monomers
Opis:
Mutagenic properties of four different diphenylthiophenes: 3,4-diphenylthiophene, 3,4-di(4'-methylphenyl)thiophene, 3,4-di(4'-methoxyphenyl)thiophene and 3,4-di(4'-pentoxyphenyl)thiophene were investigated applying the Salmonella test. The research was done on two strains of Salmonella Typhimurium: TA98 and TA100, tested in two variants: with (+S9) and without (-S9) enzymatic activation. Only one compound 3,4-di(4'-methylphenyl)thiophene showed mutagenic activity when studied with metabolic activation (+S9) and its mutagenic rate (MR) score was 3.41 for the dose of 10.00µg*plate-1. Other studied compounds did not show any mutagenic activity (+/-S9) and their MR score did not exceed the threshold value of 2.0.
Źródło:
Environmental Biotechnology; 2011, 7, 2; 65-69
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis and fungistatic activity of aryl-substituted naphthalene- and indene-2-carbonitriles
Autorzy:
Kozik, B
Burgieł, Z. J.
Sepioł, J. J.
Wilamowski, J.
Łuczyński, M. K.
Góra, M.
Powiązania:
https://bibliotekanauki.pl/articles/363192.pdf
Data publikacji:
2006
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
grzyby fitopatogenne
WWA
aktywność fungistatyczna
ochrona roślin
phytopathogenic fungi
PAHs
fungistatic activity
plant protection
Opis:
4-Aryl-substituted 1-aminonaphthalene-2-carbonitriles and 3-phenyl-1-oxo-1H-indene-2-carbonitriles were prepared in a two--step synthesis from appropriate carbonyl compounds and were evaluated in vitro for growth-inhibiting activity against some phytopathogenic fungi: Fusarium culmorum, Penicillium expansum, Botrytis cinerea and Alternaria species. The results were compared with the activity of a commercial fungicide - captan. The highest fungistatic activity was revealed by 1-amino-4-phenylnaphthalene-2-carbonitrile. An efficient synthesis of 1-amino-4-arylnaphthalene-2-carbonitriles involves condensation of diarylacetaldehydes or 1,1-diarylacetones with malonodinitrile and cyclization of the obtained aryl-ylidenemalonodinitriles in concentrated sulfuric acid. The benzannulation reaction is accompanied by a quasi-aromatic rearrangement. The indenone derivatives were synthesized in the same manner from substituted benzophenones. As the formation of five-membered ring during the cyclization of diarylmethylidenemalonodinitriles was less effective, the rearrangement was not observed.
Źródło:
Environmental Biotechnology; 2006, 2, 1; 20-25
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary study on adverse effects of phenanthrene and its methyl and phenyl derivatives in larval zebrafish, Danio rerio
Autorzy:
Wolińska, L.
Brzuzan, P.
Woźny, M.
Góra, M.
Łuczyński, M. K.
Podlasz, P.
Kolwicz, S.
Piasecka, A.
Powiązania:
https://bibliotekanauki.pl/articles/363152.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
toksyczność ostra
CYP1A
cyp1b1
geny vtg
ekspresja mRNA
stężenie nie wywołujące efektu
WWA
Real-Time qPCR
acute toxicity
cyp1a genes
cyp1b1 genes
vtg genes
mRNA expression
No Effect Concentration
PAHs
Opis:
Toxic effects of polycyclic aromatic hydrocarbons (PAHs) have been extensively studied in fish, although knowledge concerning biological activities of phenanthrene and its derivatives remains still incomplete. The aim of this work was to evaluate lethal and sublethal effects of benzo(a)pyrene, phenanthrene and phenanthrene derivatives (1-methylphenanthrene, 4-methylphenanthrene, 1-phenylphenanthrene and 4-phenylphenanthrene) on zebrafish (Danio rerio) larvae. We conducted acute toxicity test, using 96h static renewal exposure to a series of the PAH concentrations (0.05, 0.50, 5.00, 50.00µmol*l-1), to determine the No Effect Concentration (NEC) value for each compound examined. The mean NEC estimates obtained in the study were 5.16۪.45µmol*l-1 (B[a]P), 4.88۪.13µmol*l-1 (Ph), 40.24䔰.93µmol*l-1 (1P-Ph), 47.92ۭ.61µmol*l-1 (1M-Ph), 24.31۱.33µmol*l-1 (4P-Ph) and 3.11۫.01µmol*l-1 (4M-Ph) and suggested the following order of PAH toxicities on Danio rerio larvae: 4M-Ph>Ph˜B[a]P>4PPhP-Ph>1M-Ph. To gain insight into possible molecular mechanisms of apparent toxicity of phenanthrene derivatives on zebrafish larvae, we examined mRNA expression of cyp1a, cyp1b1, and vtg genes in the larvae exposed for 48h to a PAH concentration of 0.50µmol*l-1. Whereas the larvae exposed to each tested PAH displayed many developmental abnormalities (i.e. pericardial and yolk sac edema, dorsal curvature, or tail malformations), no significant upregulation of cyp1a and cyp1b1 mRNA was observed, except for zebrafish exposed to B[a]P. However, significant reduction of vtg mRNA was observed in the larvae exposed to phenanthrene and its 4P- derivative. The results may contribute to the development of a new knowledge about effects of structurally diverse phenanthrene derivatives on vertebrate organisms.
Źródło:
Environmental Biotechnology; 2011, 7, 1; 26-33
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
MicroRNA expression profiles in liver and colon of sexually immature gilts after exposure to Fusarium mycotoxins
Autorzy:
Brzuzan, P.
Wozny, M.
Wolinska-Niziol, L.
Piasecka, A.
Florczyk, M.
Jakimiuk, E.
Gora, M.
Luczynski, M.K.
Gajecki, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087983.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
To improve our knowledge of the role of microRNAs (miRs) in responses of the porcine digestive system to two Fusarium mycotoxins, zearalenone (ZEN) and deoxynivalenol (DON), we examined the expression of 7 miRs (miR-9, miR-15a, miR-21, miR-34a, miR-122, miR-125b, and miR-192), previously found to be deregulated in diseased liver and colon cells. In this study, immature gilts were exposed to NOEL doses of ZEN (40 |ig/kg/d), DON (12 |ig/kg/d), ZEN+DON (40 + 12 |ig/kg/d), and placebo (negative control group) for 7,14, 21, 28, 35, and 42 days. Before the treatment, expression levels of the selected miRs were measured in the liver, the duodenum, the jejunum, and the ascending and the descending colon of the gilts. Hierarchical clustering of the tissues by their miR expression profiles was consistent with what would be expected based on the anatomical locations and the physiological functions of the organs, suggesting that functions of the miRs are related to the specificities of the tissues in which they are expressed. A subset of 2 pairs of miRs (miR-21+miR-192 and miR-15a+miR-34a), which were assigned to two distinct clusters based on their tissue abundance, was then evaluated in the liver and the ascending and the descending colon during the treatment. The most meaningful results were obtained from the ascending colon, where a significant effect of the treatment was observed, suggesting that during the exposure to mycotoxins, the pathways involved in cell proliferation and survival were disordered. Changes in miR expression in the liver and the descending colon of the treated gilts were smaller, and were associated more with treatment duration than the exposure to ZEN, DON, or ZEN+DON. Further research should focus on identification of genes whose expression is regulated by these aberrantly expressed miRs. This should facilitate understanding of the miRNA-regulated biological effects of mycotoxins.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-25 z 31

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