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Wyświetlanie 1-15 z 134
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7
Autorzy:
Li, Y.D.
Yu, Z.D.
Bai, C.X.
Zhang, D.
Sun, P.
Peng, M.L
Liu, H.
Wang, J.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087207.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Capsid gene
PPV7
SYBR Green I real-time PCR
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 43-49
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Bending, Impact Fracture Behavior and Characteristics of Stainless Steel Clad Plates with Different Rolling Temperature
Autorzy:
An, Q.
Fan, K. Y.
Ge, Y. F.
Liu, B. X.
He, J.
Wang, S.
Chen, C. X.
Ji, P. G.
Tolochko, O.
Powiązania:
https://bibliotekanauki.pl/articles/2049433.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
stainless steel clad plate
interface characteristics
bending behavior
impact morphologies
delamination crack
Opis:
The interface characteristics, bending and impact behavior, as well as fracture characteristics of stainless steel clad plates fabricated by vacuum hot rolling at different rolling temperatures of 1100°C, 1200°C and 1300°C are investigated in detail. The interface bonding strength is gradually increased with the increasing rolling temperature due to the sufficient diffusion behavior of alloy element. The bending toughness and impact toughness are gradually decreased, while the bending strength increase with the increase of the rolling temperature, which is attributed to mechanisms of matrix softening and interface strengthening at high rolling temperature. Due to the weak interface at 1100°C, the bending and impact crack propagation path was displaced by delamination cracks, which in turn lead to reduction in stress intensity of the main crack, playing an effective role in toughening the stainless steel clad plates. Moreover, the impact fracture morphologies of clad plates show a typical ductile-brittle transition phenomenon, which is attributed to the matrix softening behavior with the increasing rolling temperature.
Źródło:
Archives of Metallurgy and Materials; 2021, 66, 1; 229-239
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of serum adiponectin and leptin in healthy perinatal dairy cows or cows with ketosis, and their effectson ketosis involved indices
Autorzy:
Shen, L.
Qian, B.
Xiao, J.
Zhu, Y.
Hussain, S.
Deng, J.
Peng, G.
Zuo, Z.
Zou, L.
Yu, S.
Ma, X.
Zhong, Z.
Ren, Z.
Wang, Y.
Liu, H.
Zhou, Z.
Cai, D.
Hu, Y.
Zong, X.
Cao, S.
Powiązania:
https://bibliotekanauki.pl/articles/2087289.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
adiponectin
dairy cows
ketosis
leptin
perinatal period
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 373-381
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chinese cabbage BrMYB34.2 transcription factor regulates indolic glucosinolates biosynthesis in Arabidopsis
Autorzy:
Zhao, Y.
Zhang, Y.
Guo, X.
Ma, Y.
Zhang, P.
Liu, H.
Liu, G.
Guo, J.
Powiązania:
https://bibliotekanauki.pl/articles/12705984.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Glucosinolates (GS) are a group of sulfur- and nitrogen-rich plant secondary metabolites that originate from amino acids and exist mainly in plants in the order Brassicales, such as Arabidopsis thaliana (Arabidopsis) and Chinese cabbage (Brassica rapa ssp. pekinensis). To date, several regulatory components responsible for GS biosynthesis have been identified in Arabidopsis. However, the functions of GS biosynthesis regulators in Chinese cabbage have not been clarified. In our current study, a putative ATR1/MYB34 orthologous gene, BrMYB34.2, was isolated from Chinese cabbage leaves. To investigate the function of this gene, we engineered Arabidopsis plants that overexpress BrMYB34.2 ectopically and phenotypic analysis was performed. Moreover, we assayed the accumulation levels of indolic GS (IGS) and aliphatic glucosinolates in transgenic plants and test the expression of key genes of IGS biosynthesis and tryptophan synthesis by Real-time quantitative PCR. And further analysed the resistance of transgenic plants in 5MT stress treatment. The results indicate that ectopic expression of the BrMYB34.2 gene in Arabidopsis was able to up-regulate the accumulation level of IGS due to the increased expression of IGS and Trp biosynthetic genes. Moreover, overexpression of BrMYB34.2 conferred Arabidopsis 5MT resistance. These results suggest that the BrMYB34.2 gene may function as one of the regulators of IGS and Trp biosynthesis in Chinese cabbage.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 1; 85-95
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development and application of isothermal amplification methods for rapid detection of F4 fimbriae producing Escherichia coli
Autorzy:
Zhao, L.Y.
Niu, J.H.
Gao, X.L.
Liu, C.N.
Liu, S.M.
Jiang, N.
Lv, X.P.
Zheng, S.M.
Powiązania:
https://bibliotekanauki.pl/articles/2087340.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Escherichia coli
F4 fimbriae
isothermal amplification
rapid detection
clinical samples
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 143-152
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of organic cultivation pattern on tomato production: plant growth characteristics, quality, disease resistance, and soil physical and chemical properties
Autorzy:
Feng, X.
Xu, Y.
Liu, D.
Peng, L.
Dong, J.
Yao, S.
Feng, Y.
Feng, Z.
Li, F.
Hu, B.
Powiązania:
https://bibliotekanauki.pl/articles/12703546.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Tomato (Solanum lycopersicum L.) is one of the world’s most important cultivated vegetable. In the traditional cultivation methods, the excessive use of pesticides and fertilizers leads to an imbalance of nutrient elements in the soil, an increase in pests and diseases and a decrease in vegetable quality and yield. In the face of increasingly serious environmental and food problems, organic agriculture is considered to be an effective solution. In this experiment, the effects of organic cultivation patterns on the growth, quality, disease resistance in tomatoes, and the physical and chemical properties of soil were studied by different treatments. The results showed that the application of effective microorganisms (EM) bio-organic fertilizer in the cultivation process can significantly improve the yield, quality, and antioxidant enzyme activity of tomato. The use of straw mulching was found to significantly increase the growth, chlorophyll content, transpiration rate, and soluble sugar content of tomatoes. The application of EM bio-organic fertilizer or straw mulching significantly increased the activity of antioxidant enzyme and the expression of LeCHI gene in tomato leaves and enhanced tomato resistance to diseases. Organic production practices were found to significantly improve the soil.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 1; 71-84
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
iTRAQ-based quantitative proteomics analysis reveals inhibitory mechanismsof the antimicrobial peptide MDAP-2 against Salmonella gallinarum
Autorzy:
Zhang, Y.
Yu, S.
Ying, X.
Jia, B.
Liu, L.
Liu, J.
Kong, L.
Pei, Z.
Ma, H.
Powiązania:
https://bibliotekanauki.pl/articles/2087299.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Salmonella gallinarum
AMP
iTRAQ
inhibitory mechanism
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 405-414
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Response of dry matter translocation and grain yield of summer maize to biodegradable film in the North China Plain
Autorzy:
Tan, D.
Guo, L.
Liu, J.
Fan, Y.
Li, Q.
Powiązania:
https://bibliotekanauki.pl/articles/2082862.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
polyethylene film
leaf area index
dry matter
transfer
yield components
North China Plain
Opis:
In the North China Plain, it is a matter of urgency to explore the feasibility of using biodegradable film to replace polyethylene film. A field experiment was conducted by covering soils with polyethylene white film, biodegradable white film, biodegradable black film, while the control remained uncovered. This study analysed the effects of using different film types on summer maize dry matter accumulation and transfer, grain yield and yield components during the 2016 and 2017 summer maize growing seasons. Results showed that, for both growing seasons, compared with non-mulching, dry matter translocation, dry matter transfer efficiency of vegetative organs and grain yield for plants following polyethylene white film and biodegradable white film treatments were always lower. However, dry matter accumulation, dry matter translocation, dry matter transfer efficiency, grain yield, and the contribution of dry matter translocation to grain yield before flowering in biodegradable black film treatments significantly increased by 21.0, 33.3, 21.4, 12.6, and 18.5%, respectively. Only the black biodegradable film could increase grain yield as determined by the 1000 kernel mass. Results indicate that black biodegradable films are a viable alternative to polyethylene film in summer maize production in the North China Plain.
Źródło:
International Agrophysics; 2020, 34, 1; 87-94
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seismic damage behaviour of aeolian sand reinforced concrete columns with I-shaped structural steel
Autorzy:
Wang, Y. H.
Wang, H. Y.
Wang, Y. P.
Zhao, M.
Qi, J.
Huo, G. Z.
Liu, P. Q.
Powiązania:
https://bibliotekanauki.pl/articles/1849772.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
piasek eoliczny
słup żelbetowy
stal konstrukcyjna
przekrój I
zachowanie
uszkodzenie sejsmiczne
model uszkodzenia
aeolian sand
column
I-shaped steel
structural steel
seismic damage
behaviour
damage model
Opis:
To promote the application of aeolian sand resources for steel-concrete composite structures, an aeolian sand reinforced concrete column with I-shaped structural steel is proposed in this study. Four specimens are designed and manufactured with different replacement rates of aeolian sand. The seismic behaviour and damage evolution process of the specimens are studied by low-cycle repeated loading tests. Based on the test results, the mechanical characteristics, failure modes, hysteresis curves, skeleton curves, energy dissipation capacity, displacement ductility, and stiffness degradation of the specimens with different replacement rates of aeolian sand are analysed. In addition, the effects of the design parameters on the seismic behaviour of the specimens are also studied. The results show that the indexes of the seismic behaviour can be significantly improved by adding steel. Moreover, a revised damage model is proposed, to better reflect the evolution law of seismic damage of aeolian sand reinforced concrete columns with steel. The proposed model can provide an important reference for seismic damage assessment of the columns.
Źródło:
Archives of Civil Engineering; 2020, 66, 4; 633-650
1230-2945
Pojawia się w:
Archives of Civil Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Anatomy and relationships of the fuxianhuiid euarthropod Guangweicaris from the early Cambrian Guanshan Biota in Kunming, Yunnan, Southwest China revisited
Autorzy:
Wu, Y.
Liu, J.
Powiązania:
https://bibliotekanauki.pl/articles/21912.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Opis:
The Guanshan Biota (Cambrian Series 2, Stage 4) is a typical Burgess Shale-type biota, and is one of the most significant Cambrian Konservat-Lagerstätten from China, in addition to the well-known Chengjiang and Kaili biotas. Guangweicaris is a relatively common arthropod from the Guanshan Biota. Based on new specimens from the Longbaoshan and Xinglongcun sections of the Wulongqing Formation in Kunming, we herein report novel features of Guangweicaris spinatus, such as the eye-bearing anterior sclerite that is associated with the head shield, as well as revised details of the trunk, including the ventral appendages. A detailed comparison of Guangweicaris, Fuxianhuia and Shankouia suggests a close affinity between Guangweicaris and Fuxianhuia, which improves our understanding of the evolution of early Cambrian euarthropods.
Źródło:
Acta Palaeontologica Polonica; 2019, 64, 3
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Catalytic oxidation of lignin to aromatics over salen-porphyrin complex as a biomimetic catalyst
Autorzy:
Lu, X.-J.
Zhou, X.-F.
Zhu, Z.-L.
Sun, Y.
Tang, K.
Lei, F.-H.
Liu, Z.-G.
Wang, T.
Powiązania:
https://bibliotekanauki.pl/articles/52771.pdf
Data publikacji:
2019
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Technologii Drewna
Źródło:
Drewno. Prace Naukowe. Doniesienia. Komunikaty; 2019, 62, 203
1644-3985
Pojawia się w:
Drewno. Prace Naukowe. Doniesienia. Komunikaty
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular cloning, recombinant expression, and purification of osteocalcin in sika deer (Cervus nippon) antler
Autorzy:
Li, X.
Liu, M.
Bai, X.
Li, Y.
Zhao, Y.
Wang, S.
Wang, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087587.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Cervus nippon
osteocalcin
molecular cloning
expression
purification
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 143-150
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Quantitative processing of situation awareness for autonomous ships navigation
Autorzy:
Zhou, X. Y.
Liu, Z. J.
Wu, Z. L.
Wang, F. W.
Powiązania:
https://bibliotekanauki.pl/articles/117424.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
autonomous vessel
autonomous ship
autonomous ships navigation
situation awareness (SA)
quantitative processing
navigation safety
remotely controlled vessel
maritime unmanned navigation through intelligence in networks (MUNIN)
Opis:
The first ever attempt at fully autonomous dock-to-dock operation has been tested and demonstrated successfully at the end of 2018. The revolutionary shift is feared to have a negative impact on the safety of navigation and the getting of real-time situation awareness. Especially, the centralized context onboard could be changed to a distributed context. In navigation safety domain, monitoring, control, assessment of dangerous situations, support of operators of decision-making support system should be implemented in real time. In the context of autonomous ships, decision-making processes will play an important role under such ocean autonomy, therefore the same technologies should consist of adequate system intelligence. At the same time, situation awareness is the key element of the decision-making processes. Although there is substantial research on situation awareness measurement techniques, they are not suitable to directly execute quantitative processing for the situation awareness of autonomous ships navigation. Hence, a novel quantitative model of situation awareness is firstly proposed based on the system safety control structure of remotely controlled vessel. The data source is greatly limited, but the main result still indicates that the probability of operator lose adequate situation awareness of the autonomous ship is significantly higher than the conventional ship. Finally, the paper provides a probabilistic theory and model for high-level abstractions of situation awareness to guide future evaluation of the navigation safety of autonomous ships.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2019, 13, 1; 25-31
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Relationship of adiponectin, leptin, visfatin and IGF-1 in cow’s venous blood and venous cord blood with calf birth weight
Autorzy:
Shen, L.
Zhu, Y.
Xiao, J.
Deng, J.
Peng, G.
Zuo, Z.
Yu, S.
Ma, X.
Zhong, Z.
Ren, Z.
Zhou, Z.
Liu, H.
Zong, X.
Cao, S.
Powiązania:
https://bibliotekanauki.pl/articles/2087491.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
adiponectin
leptin
IGF-1
birth weights
cow
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 541-548
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 134

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