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Wyświetlanie 1-15 z 200
Tytuł:
The study on bactericidal effect and ultrastructural alterations of chlorocresol nanoemulsion disinfectant against Staphylococcus aureus
Autorzy:
Zhang, Y.F.
Sun, Y.W.
Liu, X.H.
An, Z.X.
Yang, X.F.
Powiązania:
https://bibliotekanauki.pl/articles/16647452.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
chlorocresol
nanoemulsion
disinfectant
Staphylococcus aureus
bactericidal effect
ultrastructure
Opis:
Chlorocresol nanoemulsion disinfectant (CND) is an environmental disinfectant prepared with nanoemulsion as its drug carrier. This study aimed to investigate the bactericidal effect of CND on Staphylococcus aureus ( S. aureus) and its effect on bacterial ultrastructure. The neutralizing effect of CND against S. aureus was first screened by suspension quantitative evaluation experiment procedure of neutralizer. Disinfection performance was evaluated by the determination of Minimal Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), quantitative bactericidal experiment, and comparative experiment of disinfection performance between 0.1% CND and 0.1% chlorocresol aqueous solution. Meanwhile, the effect of CND on the ultrastructure of S. aureus was investigated with scanning electron microscope (SEM) and transmission electron microscope (TEM) to preliminarily explore the bactericidal mechanism. The results showed that 3% Tween-80 in PBS could be screened as the neutralizer of CND against S. aureus. MIC and MBC were 100 μg/mL and 200 μg/mL, respectively. The bactericidal rates were all 100% when 0.06% and 0.08% disinfectant acted for 15 and 5 min, respectively. Furthermore, compared with 0.1% chlorocresol aqueous solution, the bactericidal effect of 0.1% CND was significantly enhanced (p<0.01). After treatment with CND for 10 min, SEM observation showed that the morphology of S. aureus cells were changed and the integrity destroyed. TEM observation showed that the cell shape changed, and the structures of the cell wall, cell membrane and cytoplasm were damaged in varying degrees. CND showed the strong bactericidal effect on S. aureus and could cause ultrastructure alterations of S. aureus.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 1; 13-20
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative analysis of CpG islands in two genotypes of African swine fever virus
Autorzy:
Yu, Y.-Y.
X, M.-S.
Liu, Q.
Powiązania:
https://bibliotekanauki.pl/articles/16539329.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
African Swine Fever Virus (ASFV)
genotype
DNA methylation
CpG island
Opis:
African swine fever (ASF) is an acute, hemorrhagic, and devastating viral infectious disease that causes important economic losses to the swine industry. Currently, there are no effective vaccines or drugs available. Epigenetic mechanisms, especially cytosine methylation of cytosine-phosphate-guanine (CpG) islands, have a significant impact on the life cycle of several viruses. Hence, drugs targeting DNA methylation may potentially be used for the treatment of ASF. Here, we selected the inner core, core shell, inner membrane, capsid, and external envelope membrane, to analyze the characteristics of CpG islands in the ASF virus (ASFV) genomes. Furthermore, we analyzed the promoters and CpG islands in the upstream regions of these genes. Results showed that the CpG islands of seven genes were conserved in the genomes of two genotype of ASFV strains, whereas the CpG islands of other genes were relatively conserved (ASFV strains differed mainly in the quantity of CpG islands). The different distribution of CpG islands in the genomes of different ASFV strains may affect their methylation status, which may in turn affect the regulation of viral gene expression, leading to different clinical outcomes. In addition, the predicted promoter regions based on the upstream sequences of most genes overlapped with CpG island positions. Methylation of the binding sites of the promoter regions inhibits the binding of the transcription factors to the promoters, thus inhibiting the activation of the promoters and limiting the synthesis of viral proteins. The results of this study provide a basis for exploring new antiviral therapeutic strategies from an epigenetic perspective.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 3; 455-462
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of organic supplementation to reduced rates of chemical fertilization on soil fertility of Zanthoxylum armatum
Autorzy:
Saba, T.
Liu, W.
Wang, J.
Saleem, F.
Kang, X.
Hui, W.
Gong, W.
Li, H.
Powiązania:
https://bibliotekanauki.pl/articles/2130175.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
fertilization
soil fertility
nutrient availability
organic manure
culturable microbes
Opis:
Soil acidification, fertility depletion, food insecurity, and environmental pollution all are consequences of the extensive use of chemical fertilizers (CF) in intensively managed plantations. Recent studies have shown that incorporating organic manure (OM) to partially replace CF can help to maintain productivity and soil health. There are no reports on integrated fertilizer management of Zanthoxylum armatum, for optimal ecological services and economic profitability. A pot experiment with Z. armatum was performed to investigate the possibility of incorporating OM in reducing CF use, enhancing soil fertility, and increasing plant growth. For this, seven treatments were designed: NPK, PK, NP, NK, OM, MNPK (50%NPK+50%OM), and control (CK, no fertilizer application). The results showed that NPK application significantly (P < 0.05) increased plant growth, soil nitrate-N, ammonium-N and available K compared to CK. However conventional CF application induced soil acidification. OM application raised the soil pH from 5.50 (CK) to 6.58 and significantly enhanced soil fertility by increasing alkali-hydrolysable N (2.3 times), ammonium-N (1.4 times), available P (4.3 times), the activity of invertase (1.5 times), catalase (2.8 times), acid phosphatase (1.5 times), and count of soil culturable microbes (2.9 times) compared to CK. Conversely, sole OM application did not result in optimum plant growth. Integrating OM with NPK (MNPK), on the other hand, not only provided the highest growth comprehensive value (0.84), but also substantially higher soil fertility comprehensive value (0.71) than traditional CF treatments. Correlation analysis also indicated a positive and significant correlation between soil microbes, enzyme activities and available nutrients. Therefore, OM+NPK could be an optimum measure to get maximum benefits regarding soil fertility, growth of Z. armatum, fertilizer savings and sustainable agroecology.
Źródło:
Dendrobiology; 2022, 87; 123-136
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7
Autorzy:
Li, Y.D.
Yu, Z.D.
Bai, C.X.
Zhang, D.
Sun, P.
Peng, M.L
Liu, H.
Wang, J.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087207.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Capsid gene
PPV7
SYBR Green I real-time PCR
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 43-49
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Bending, Impact Fracture Behavior and Characteristics of Stainless Steel Clad Plates with Different Rolling Temperature
Autorzy:
An, Q.
Fan, K. Y.
Ge, Y. F.
Liu, B. X.
He, J.
Wang, S.
Chen, C. X.
Ji, P. G.
Tolochko, O.
Powiązania:
https://bibliotekanauki.pl/articles/2049433.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
stainless steel clad plate
interface characteristics
bending behavior
impact morphologies
delamination crack
Opis:
The interface characteristics, bending and impact behavior, as well as fracture characteristics of stainless steel clad plates fabricated by vacuum hot rolling at different rolling temperatures of 1100°C, 1200°C and 1300°C are investigated in detail. The interface bonding strength is gradually increased with the increasing rolling temperature due to the sufficient diffusion behavior of alloy element. The bending toughness and impact toughness are gradually decreased, while the bending strength increase with the increase of the rolling temperature, which is attributed to mechanisms of matrix softening and interface strengthening at high rolling temperature. Due to the weak interface at 1100°C, the bending and impact crack propagation path was displaced by delamination cracks, which in turn lead to reduction in stress intensity of the main crack, playing an effective role in toughening the stainless steel clad plates. Moreover, the impact fracture morphologies of clad plates show a typical ductile-brittle transition phenomenon, which is attributed to the matrix softening behavior with the increasing rolling temperature.
Źródło:
Archives of Metallurgy and Materials; 2021, 66, 1; 229-239
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A numerical model for impacts of left-turn non-motorized vehicles on through lane capacity metrics
Autorzy:
He, Lieyun
Lin, Xinming
Liu, Qiang
Tao, Jason X,
Powiązania:
https://bibliotekanauki.pl/articles/1833648.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
traffic design
signalized intersection
permissive phase
traffic lane capacity
regression analysis
projektowanie ruchu
skrzyżowanie sygnalizowane
faza permisywna
przepustowość pasów ruchu
analiza regresji
Opis:
There is a conflict between through motor vehicles and the left-turn non-motorized vehicles, and the capacity of straight-line motor vehicles decreases. This study analyzes the impacts of left-turn non-motorized vehicles on the capacity of through motor vehicle lanes. A correction coefficient model for calculating the reduced capacity of through motor vehicle lanes has been developed based on analysis of the conflicting points at an intersection and the negative exponential function of traffic flow distribution. With consideration of intersection geometric design, channelization, and traffic characteristics, the cor-rection coefficient model was further enhanced by regression to capture the impacts of left-turn non-motorized vehicles from the same and the opposite directions. A simulation with VISSIM is used to validate the developed model. It shows that the calculated capacity from the correction coefficient model is close to the simulation results. The experiment indicates that the derived model is highly accurate in calculating the capacity of through motor vehicle lanes and has potential application for situations of mixed traffic in China. The study shows that the capacity of a through traffic lane at the permitted phase decreases with the increase of left-turning non-motorized vehicles, and the impact of left-turning non-motorized vehicles from the same direction is more significant. The results show that the traffic capacity of straight-line motor vehicle decreases with the increase of the left-turn non-motorized vehicles flow rate and the influence of the left-turn non-motor vehicle is more obvious. It is suggested that in practice, the correction coefficient of non-motor vehicle on the left turn should be 0.88, and the correction coefficient on the left turn should be 0.95, respectively. The study recommends coefficient values for both non-motorized vehicles from the same and opposite directions for use in real applications.
Źródło:
Archives of Transport; 2020, 55, 3; 7-16
0866-9546
2300-8830
Pojawia się w:
Archives of Transport
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of root system architecture affected by swarming behavior
Autorzy:
Li, S.
Yu, W.
Liu, X.
Wang, M.
Powiązania:
https://bibliotekanauki.pl/articles/1078927.pdf
Data publikacji:
2020
Wydawca:
Instytut Ogrodnictwa
Tematy:
root system architecture
soil nutrient
interaction
root system
root growth
dynamic growth
swarming
behaviour
Opis:
The root system architecture (RSA) displays complex morphological characteristics because of diverse root growth behaviors. Recent studies have revealed that swarming behavior among roots is particularly important for RSA to adapt to environmental stimuli. However, few models are proposed to simulate RSA based on swarming behavior of roots. To analyze plasticity of RSA affected by swarming behavior, we propose viewing it as a swarm of single roots. A swarming behavior model is proposed by considering repulsion, alignment, and preference of individual single roots. Then, the swarming behavior model is integrated into a simple and generic RSA model (called ArchiSimple). Lastly, characteristics of RSA affected by swarming behavior model and non-swarming behavior model are compared and analyzed under three different virtual soil sets. The characteristics of RSA (such as primary root length, lateral root length, lateral roots, and resource uptake) are significantly promoted by swarming behavior. Root system distributions can also be greatly affected by swarming behavior. These results show that root foraging and exploration in soil can be regarded as collective behavior of individual single root.
Źródło:
Journal of Horticultural Research; 2020, 28, 1; 1-12
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of serum adiponectin and leptin in healthy perinatal dairy cows or cows with ketosis, and their effectson ketosis involved indices
Autorzy:
Shen, L.
Qian, B.
Xiao, J.
Zhu, Y.
Hussain, S.
Deng, J.
Peng, G.
Zuo, Z.
Zou, L.
Yu, S.
Ma, X.
Zhong, Z.
Ren, Z.
Wang, Y.
Liu, H.
Zhou, Z.
Cai, D.
Hu, Y.
Zong, X.
Cao, S.
Powiązania:
https://bibliotekanauki.pl/articles/2087289.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
adiponectin
dairy cows
ketosis
leptin
perinatal period
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 373-381
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chinese cabbage BrMYB34.2 transcription factor regulates indolic glucosinolates biosynthesis in Arabidopsis
Autorzy:
Zhao, Y.
Zhang, Y.
Guo, X.
Ma, Y.
Zhang, P.
Liu, H.
Liu, G.
Guo, J.
Powiązania:
https://bibliotekanauki.pl/articles/12705984.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Glucosinolates (GS) are a group of sulfur- and nitrogen-rich plant secondary metabolites that originate from amino acids and exist mainly in plants in the order Brassicales, such as Arabidopsis thaliana (Arabidopsis) and Chinese cabbage (Brassica rapa ssp. pekinensis). To date, several regulatory components responsible for GS biosynthesis have been identified in Arabidopsis. However, the functions of GS biosynthesis regulators in Chinese cabbage have not been clarified. In our current study, a putative ATR1/MYB34 orthologous gene, BrMYB34.2, was isolated from Chinese cabbage leaves. To investigate the function of this gene, we engineered Arabidopsis plants that overexpress BrMYB34.2 ectopically and phenotypic analysis was performed. Moreover, we assayed the accumulation levels of indolic GS (IGS) and aliphatic glucosinolates in transgenic plants and test the expression of key genes of IGS biosynthesis and tryptophan synthesis by Real-time quantitative PCR. And further analysed the resistance of transgenic plants in 5MT stress treatment. The results indicate that ectopic expression of the BrMYB34.2 gene in Arabidopsis was able to up-regulate the accumulation level of IGS due to the increased expression of IGS and Trp biosynthetic genes. Moreover, overexpression of BrMYB34.2 conferred Arabidopsis 5MT resistance. These results suggest that the BrMYB34.2 gene may function as one of the regulators of IGS and Trp biosynthesis in Chinese cabbage.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 1; 85-95
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development and application of isothermal amplification methods for rapid detection of F4 fimbriae producing Escherichia coli
Autorzy:
Zhao, L.Y.
Niu, J.H.
Gao, X.L.
Liu, C.N.
Liu, S.M.
Jiang, N.
Lv, X.P.
Zheng, S.M.
Powiązania:
https://bibliotekanauki.pl/articles/2087340.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Escherichia coli
F4 fimbriae
isothermal amplification
rapid detection
clinical samples
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 143-152
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of organic cultivation pattern on tomato production: plant growth characteristics, quality, disease resistance, and soil physical and chemical properties
Autorzy:
Feng, X.
Xu, Y.
Liu, D.
Peng, L.
Dong, J.
Yao, S.
Feng, Y.
Feng, Z.
Li, F.
Hu, B.
Powiązania:
https://bibliotekanauki.pl/articles/12703546.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Tomato (Solanum lycopersicum L.) is one of the world’s most important cultivated vegetable. In the traditional cultivation methods, the excessive use of pesticides and fertilizers leads to an imbalance of nutrient elements in the soil, an increase in pests and diseases and a decrease in vegetable quality and yield. In the face of increasingly serious environmental and food problems, organic agriculture is considered to be an effective solution. In this experiment, the effects of organic cultivation patterns on the growth, quality, disease resistance in tomatoes, and the physical and chemical properties of soil were studied by different treatments. The results showed that the application of effective microorganisms (EM) bio-organic fertilizer in the cultivation process can significantly improve the yield, quality, and antioxidant enzyme activity of tomato. The use of straw mulching was found to significantly increase the growth, chlorophyll content, transpiration rate, and soluble sugar content of tomatoes. The application of EM bio-organic fertilizer or straw mulching significantly increased the activity of antioxidant enzyme and the expression of LeCHI gene in tomato leaves and enhanced tomato resistance to diseases. Organic production practices were found to significantly improve the soil.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 1; 71-84
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
iTRAQ-based quantitative proteomics analysis reveals inhibitory mechanismsof the antimicrobial peptide MDAP-2 against Salmonella gallinarum
Autorzy:
Zhang, Y.
Yu, S.
Ying, X.
Jia, B.
Liu, L.
Liu, J.
Kong, L.
Pei, Z.
Ma, H.
Powiązania:
https://bibliotekanauki.pl/articles/2087299.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Salmonella gallinarum
AMP
iTRAQ
inhibitory mechanism
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 405-414
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New data on the anatomy of fuxianhuiid arthropod Guangweicaris spinatus from the lower Cambrian Guanshan Biota, Yunnan, China
Autorzy:
Chen, H.
Legg, D.A.
Zhai, D.-Y.
Liu, Y.
Hou, X.-G.
Powiązania:
https://bibliotekanauki.pl/articles/2082130.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Tematy:
Arthropoda
Euarthropoda
Fuxianhuiida
Fuxianhuiidae
Guanshan Biota
Cambrian
Asia
Opis:
The fuxianhuiid arthropod Guangweicaris spinatus, from the lower Cambrian (Series 2, Stage 4), Guanshan Biota (Wulongqing Member, Canglangpu Formation), located in the vicinity of Kunming (Yunnan Province, southwest China), is redescribed based on new specimens and a re-examination of previously described material. A more complete overview of its morphology is given. Newly recognised features include: (i) a medial cephalic bulge; (ii) a tripartite hypostome; (iii) a pair of specialized post-antennal appendage (SPA); (iv) a putative telson; (v) two pairs of spines on the posteroventral margin of the terminal abdominal segment. This information is used to provide an emended diagnosis of the family Fuxianhuiidae, and the genus Guangweicaris.
Źródło:
Acta Palaeontologica Polonica; 2020, 65, 1; 139-148
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Telomerase enhances osteogenic ifferentiation of sheep bone marrow mesenchymal stem cells (BMSCs) by up-regulating PI3K/Akt pathway in vitro
Autorzy:
Zhu, X.
Zhou, L.
Liu, Z.
Chen, X.
Wei, L.
Zhang, Z.
Liu, Y.
Zhu, Y.
Wang, Y.
Yang, X.
Han, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087304.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bone marrow mesenchymal stem cells
telomerase
proliferation
osteogenic differentiation
PI3K/Akt signalling pathway
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 359-372
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
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