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Tytuł:
In vitro co-stimulation of anti-tumor activity by soluble B7 molecules
Autorzy:
He, Wei
Hu, Zhong-Bo
Liu, Fang
Feng, Xian-Qi
Zou, Ping
Powiązania:
https://bibliotekanauki.pl/articles/1041178.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
immunotherapy
tumor escape
gene fusion
recombinant fusion proteins
Opis:
In order to investigate the anti-tumor activity of a soluble B7-1/immunoglobulin G fusion protein and explore an effective method to eliminate immune escape of tumor cells, a recombinant vector encoding this fusion protein was constructed and constitutively expressed in Chinese hamster ovary cells. After purification with protein G affinity chromatography, the soluble fusion protein was tested for bioactivity. Results showed that the fusion protein could significantly increase the density of B7-1 molecules on WEHI-3 cells, a mouse leukemia cell line. Through allogeneic mixed lymphocyte tumor cultures, it was demonstrated that, with the presence of the first signal, it could also significantly enhance T cell activation and killing activity against WEHI-3 cells and interleukin-2 secretion by activated mouse T lymphocytes. The conclusion can be drawn that the soluble B7-IgG fusion protein has a potent capacity to generate or enhance anti-tumor immune response in vitro, and its clinical value deserves further investigation.
Źródło:
Acta Biochimica Polonica; 2006, 53, 4; 807-813
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase/reductase 9
Autorzy:
Liu, Shen
Huang, Chaoqun
Li, Dawei
Ren, Weihua
Zhang, Haoxing
Qi, Meiyan
Li, Xin
Yu, Long
Powiązania:
https://bibliotekanauki.pl/articles/1041143.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
liver
clone
short-chain dehydrogenase/reductase
Opis:
We report here the cloning and characterization of a novel human short-chain dehydrogenases/reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.
Źródło:
Acta Biochimica Polonica; 2007, 54, 1; 213-218
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Estimation of wind erosion rates of Mongolian Plateau by using 137Cs tracing technique
Ocena intensywnosci erozji eolicznej Plaskowyzu Mongolskiego z wykorzystaniem techniki oznaczania 137Cs
Autorzy:
Qi, Y
Liu, J.
Shi, H.
Zhuang, D.
Hu, Y.
Powiązania:
https://bibliotekanauki.pl/articles/81677.pdf
Data publikacji:
2010
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
estimation
wind erosion
erosion rate
Mongolian Plateau
137Cs tracing technique
Opis:
Wind erosion is one of the major environmental problems in semi-arid and arid regions. Here we established a transect from northwest (Tariat, Mongolia) to southeast (Xilingol, Inner Mongolia of China) across the Mongolian Plateau, and selected eight sampling sites along the transect. We then estimated the soil wind erosion rates by using the 137Cs tracing technique and examined their spatial dynamics. In the Mongolia section (from Tariat to Sainshand), the wind erosion rate increased gradually with vegetation type and climatic regimes; the wind erosion process was controlled by physical factors such as annual precipitation and vegetation coverage, etc. While in the China section (Inner Mongolia), the wind erosion rates of Xilinhot, Zhengxiangbai Banner and Taipusi Banner were thrice as much as those of Bayannur of Mongolia, although these four sites were all dominated by typical steppe. Besides the physical factors, higher population density and livestock carrying level should be responsible for the higher wind erosion rates in these regions of Inner Mongolia.
W pracy przedstawiono wyniki badań intensywności erozji eolicznej Płaskowyżu Mongolskiego, określonej na podstawie ośmiu prób gruntu pobranego wzdłuż transektu o długości około 1400 km, przebiegającego przez teren Mongolii i Chin. Do oceny intensywności erozji wykorzystano technikę oznaczania 137Cs w wierzchnich warstwach gruntu do głębokości 30 cm. Stwierdzono, że intensywność erozji w analizowanym transekcie od północnego-zachodu stopniowo wzrasta wraz ze zmianami reżimu klimatycznego (zmniejszania się rocznej wysokości opadów), a także zmianami pokrycia roślinnego. Chińska część Płaskowyżu o charakterze stepowym jest trzykrotnie bardziej intensywnie erodowana. Oprócz czynników fizycznych, na intensywność procesu erozji eolicznej wpływa większa gęstość zaludnienia Płaskowyżu na terenie Chin, a także większe pogłowie zwierząt hodowlanych.
Źródło:
Annals of Warsaw University of Life Sciences - SGGW. Land Reclamation; 2010, 42, 1; 197-208
0208-5771
Pojawia się w:
Annals of Warsaw University of Life Sciences - SGGW. Land Reclamation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis, Crystal Structure, and Properties of a Novel, Highly Sensitive Energetic, Coordination Compound: Iron (II) Carbohydrazide Perchlorate
Autorzy:
Liu, R.
Zhou, Z.
Qi, S.
Yang, L.
Wu, B.
Huang, H.
Zhang, T.
Powiązania:
https://bibliotekanauki.pl/articles/358343.pdf
Data publikacji:
2013
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
iron (II) carbohydrazide perchlorate
energetic material
high
sensitivity
DFT
energy gap
Opis:
A single crystal of iron (II) carbohydrazide perchlorate [FeII (CHZ)3](ClO4)2 (FeCP), a novel, lead-free, energetic coordination compound, was synthesized and its structure determined by X-ray single crystal diffraction for the frst time. The crystal belongs to the monoclinic system P2(1)/n space group, with a = 1.0066(2) nm, b = 0.8458(2) nm, c = 2.1194(4) nm, β = 100.693(3)° and Z = 4. The central Fe(II) ion is coordinated to three bidentate carbohydrazide units through the carbonyl oxygen atom and an amino nitrogen atom, forming a six-coordinated, non-centrosymmetric complex cation. The thermal analyses by differential scanning calorimetry and thermogravimetry show that the onset temperature of thermal decomposition (152.7 °C) and the critical temperature of thermal explosion of FeCP (161.2 °C) are both much lower than those of other transition metal carbohydrazide perchlorate compounds, and also those of some other primary explosives in service. FeCP has a high enthalpy of combustion, as measured by oxygen bomb calorimetry. The impact, friction and fame sensitivity tests indicate that FeCP is extremely sensitive and hazardous. Unexpected explosions occurred even during the operational processes. In order to explore the intrinsic cause of these explosions, theoretical calculations of the orbital energies were performed based on DTF. These results reveal that the impact sensitivity is positively correlated with the energy gap between HOMO and LUMO: the smaller energy gap results in the higher impact sensitivity.
Źródło:
Central European Journal of Energetic Materials; 2013, 10, 1; 17-36
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mixed leaf litter decomposition and N, P release with a focus on Phyllostachys edulis (Carriere) J. Houz. forest in subtropical southeastern China
Autorzy:
Shi, L.
Fan, S.
Jiang, Z.
Qi, L.
Liu, G.
Powiązania:
https://bibliotekanauki.pl/articles/56692.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
leaf litter
decomposition
nitrogen
phosphorus
Phyllostachys edulis
moso bamboo
tortoise-shell bamboo zob.moso bamboo
forest
subtropical area
China
Opis:
As an important non-wood forest product and wood substitute, Moso bamboo grows extremely rapidly and hence acquires large quantities of nutrients from the soil. With regard to litter decomposition, N and P release in Moso bamboo forests is undoubtedly important; however, to date, no comprehensive analysis has been conducted. Here, we chose two dominant species (i.e., Cunninghamia lanceolata and Phoebe bournei), in addition to Moso bamboo, which are widely distributed in subtropical southeastern China, and created five leaf litter mixtures (PE100, PE80PB20, PE80CL20, PE50PB50 and PE50CL50) to investigate species effects on leaf litter decomposition and nutrient release (N and P) via the litterbag method. Over a one-year incubation experiment, mass loss varied significantly with litter type (P < 0.05). The litter mixtures containing the higher proportions (>80%) of Moso bamboo decomposed faster; the remaining litter compositions followed Olson's decay mode well (R2 > 0.94, P < 0.001). N and P had different patterns of release; overall, N showed great temporal variation, while P was released from the litter continually. The mixture of Moso bamboo and Phoebe bournei (PE80PB20 and PE50PB50) showed significantly faster P release compared to the other three types, but there was no significant difference in N release. Litter decomposition and P release were related to initial litter C/N ratio, C/P ratio, and/or C content, while no significant relationship between N release and initial stoichiometric ratios was found. The Moso bamboo-Phoebe bournei (i.e., bamboo-broadleaved) mixture appeared to be the best choice for nutrient return and thus productivity and maintenance of Moso bamboo in this region.
Źródło:
Acta Societatis Botanicorum Poloniae; 2015, 84, 2
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stability control of propeller Autonomous Underwater Vehicle based on combined sections method
Autorzy:
Qi, D.
Feng, J.
Liu, A.
Hu, J.
Xu, H.
Li, Y.
Ashraf, M. A.
Powiązania:
https://bibliotekanauki.pl/articles/259119.pdf
Data publikacji:
2015
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
propeller AUV
dynamic model
stability control
simulation
Opis:
Learning from the motion principle of quadrotor, a symmetric propeller AUV, which has small size and low velocity is designed. Compared with the AUV equipped with rudders, it has better maneuverability and manipulation at low velocity. According to the Newton-Euler method, the 6 DOF kinematic model and dynamic model of the propeller AUV are established. A stability controller that consists of 3 different PID controllers is designed. It makes the depth and attitude angle as trigger conditions, and the relevant controller is chosen in different moving process. The simulation experiments simulate ideal motion state and disturbed motion state, and experiments results show that the stability controller based on combined sections method can make the best of mature technology of PID, and meet the control requirements in different stages. It has a higher respond speed and accuracy, improving the stability of the propeller AUV under the disturbance of complex ocean currents.
Źródło:
Polish Maritime Research; 2015, S 1; 157-162
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The presence of Chlamydia phage PhiCPG1 capsid protein VP1 genes and antibodies in patients infected with Chlamydia trachomatis
Autorzy:
Ma, Jingyue
Liu, Yuan
Liu, Yuanjun
Li, Lingjie
Hou, Shuping
Gao, Xibo
Qi, Manli
Liu, Quanzhong
Powiązania:
https://bibliotekanauki.pl/articles/1038770.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Chlamydia trachomatis
Chlamydia bacteriophage
capsid protein
VP1
Opis:
Chlamydia phage PhiCPG1 has been found in Chlamydia caviae in a guinea pig model for inclusion conjunctivitis, raising the possibility that Chlamydia phage is also present in patients infected with C. trachomatis (Ct). In the present study, we assayed for presence of Chlamydia phage capsid protein VP1 genes and antibodies in 84 non-Ct controls and 206 Ct patients using an enzyme-linked immunoassay (ELISA), followed by verification with Western blot. None of the subjects were exposed to an antibiotic treatment or had a C. pneumoniae infection. The VP1 antibody test was positive in both, the ELISA and Western blot assay, in 4 Ct patients. PCR amplification experiments revealed presence of the VP1 gene in 5 Ct patients. The results suggest that Chlamydia phage capsid protein VP1 may exist in some Ct patients.
Źródło:
Acta Biochimica Polonica; 2016, 63, 3; 501-504
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Horizontal transfer and functional evaluation of high pathogenicity islands in Avian Escherichia coli
Autorzy:
Tu, J.
Qi, K.
Song, X.
Xue, T.
Ji, H.
Shao, Y.
Liu, H.
Zhou, X.
Zhu, L.
Powiązania:
https://bibliotekanauki.pl/articles/30119.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
High pathogenicity islands (HPIs) in Escherichia coli encode genes that are primarily involved in iron uptake and regulation, and confer virulence and pathogenicity. The aim of this study was to investigate the transfer of HPIs in avian E. coli and identify the function of HPI in the acceptor strain. The HPI transfer strain was obtained under conditions of low temperature and low iron abundance, and the donor and acceptor strains were confirmed. E. coli HPIs are transferred by horizontal gene transfer events, which are likely mediated primarily by homologous recombination in HPI-adjacent sequences. Assays for biological activity and pathogenicity changes in the acceptor strain indicated that HPIs might not be involved in pathogenesis in avian E. coli, and thus the main function of HPIs in this strain of bacteria may be to regulate iron nutrition.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lipopolysaccharide accelerates fine particulate matter-induced cell apoptosis in human lung bronchial epithelial cells
Autorzy:
Ru, Qin
Xiong, Qi
Chen, Lin
Tian, Xiang
Yue, Kai
Ma, Baomiao
Liu, Lu
Wu, Rihui
Xu, Congyue
Pi, Mingshan
Li, Chaoying
Powiązania:
https://bibliotekanauki.pl/articles/2161782.pdf
Data publikacji:
2017-11-03
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
Apoptosis
cytotoxicity
inflammation
fine particulate matter
free radicals
bronchial epithelial cells
Opis:
Objectives The aim of the study has been to investigate the effect of the Standard Reference Material of fine particulate matter (SRM 2786) on cytotoxicity and apoptosis in human lung bronchial epithelial cells (16HBE cells). Whether the lipopolysaccharide (LPS)-induced inflammation could further accelerate cell apoptosis induced by SRM 2786 stimulation has also been determined. Material and Methods 16HBE cells were exposed to various doses of SRM 2786 with or without LPS. The following parameters: cytotoxicity, apoptotic rate, Bax/Bcl-2 expression, nitric oxide (NO) production, and reactive oxygen species (ROS) generation were measured. Results The results have shown that SRM 2786 induces cell damage and apoptosis of 16HBE cells as demonstrated by significant decrease in expression of Bcl-2 and increase in expression of Bax. When compared with the control cells, the apoptotic rate of cells treated by 500 μg/ml of SRM 2786 increased from 2.43±0.21% to 43.96±2.95% (p < 0.01). Further, there was an elevated production of NO and ROS post SRM 2786 treatment. The level of NO in cells treated with 500 μg/ml of SRM 2786 was 18.33±1.02 μmol/l whereas that of control cells was 1.58±0.31 μmol/l (p < 0.01). When compared with the control group, the level of intracellular ROS increased by 24% after treatment with 500 μg/ml of SRM 2786 (p < 0.05). In addition, LPS pre-treatment may accelerate cell apoptosis by increasing generation of NO and ROS followed by SRM 2786 stimulation. When compared to cells treated with 125 μg/ml of SRM 2786 alone, the levels of NO and ROS in cells pretreated with LPS increased by 28% and 11.6%, respectively (p < 0.05), and the apoptotic rate increased from 34.62±4.44% to 54.11±3.34% (p < 0.01). Conclusions These findings have suggested that in vitro exposure to SRM 2786 could induce 16HBE cells apoptosis probably by means of the mechanism involving the generation of free radicals, while the degree of apoptosis would be further aggravated under inflammation condition. Int J Occup Med Environ Health 2018;31(2):173–183
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2018, 31, 2; 173-183
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Looped-chain-based active current sharing strategy in DC microgrids
Autorzy:
Li, D.
Liu, Y.
Gu, Y.
Qi, H.
Powiązania:
https://bibliotekanauki.pl/articles/141109.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
active current sharing
DC microgrids
looped control diagram
Opis:
The integration of renewable energy sources in modern electric grids have drawn increasing attention nowadays. In order to effectively manage large-scale renewable energy sources and achieve flexible and efficient operation, the concept of microgrids have been proposed. Considering the nature of DC outputs in many distributed energy resources (DERs), DC microgrids have been extensively studied in the past years. Among the operational issues in DC microgrids, current sharing issues have become an important topic since it is highly relevant to the operation of DC microgrids. By adopting a proper design of current sharing strategy in DC microgrids, the current rating violations in each interface converter can be successfully avoided. In this paper, a looped-chainbased active current sharing strategy is proposed to realize high accuracy current sharing in DC microgrids. In particular, the output current is shared between the neighboring interface converters. Hence, following a clockwise or counter-clockwise order, a loopedchain- based control diagram can be established to share the reference value of the output current. A final status in the whole DC microgrid is that the output current of every interface converter is equalized. Hence, the desired current sharing objective can be satisfied. A MATLAB simulation model is established to verify the proposed loopedchain- based active current sharing strategy in DC microgrids.
Źródło:
Archives of Electrical Engineering; 2017, 66, 3; 559-569
1427-4221
2300-2506
Pojawia się w:
Archives of Electrical Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on Friction and Wear Characteristics of Aluminum Alloy Hydraulic Valve Body and Its Antiwear Mechanism
Autorzy:
Rong, L.
Lunjun, Ch.
Ming, Su
Qi, Z.
Yong, Liu
Powiązania:
https://bibliotekanauki.pl/articles/382468.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
aluminum alloy
hydraulic valve body
oil leakage
friction and wear
stop aluminium
korpus zaworu hydraulicznego
wycieki oleju
tarcie i zużycie
Opis:
In order for the working status of the aluminum alloyed hydraulic valve body to be controlled in actual conditions, a new friction and wear design device was designed for the cast iron and aluminum alloyed valve bodies comparison under the same conditions. The results displayed that: (1) The oil leakage of the aluminum alloyed hydraulic valve body was higher than the corresponding oil leakage of the iron body during the initial running stage. Besides during a later running stage, the oil leakage of the aluminum alloyed body was lower than corresponding oil leakage of the iron body; (2) The actual oil leakage of different materials consisted of two parts: the foundation leakage that was the leakage of the valve without wear and wear leakage that was caused by the worn valve body; (3) The aluminum alloyed valve could rely on the dust filling furrow and melting mechanism that led the body surface to retain dynamic balance, resulting in the valve leakage preservation at a low level. The aluminum alloy modified valve body can meet the requirements of hydraulic leakage under pressure, possibly constituting this alloy suitable for hydraulic valve body manufacturing.
Źródło:
Archives of Foundry Engineering; 2017, 17, 1; 207-215
1897-3310
2299-2944
Pojawia się w:
Archives of Foundry Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chlamydial genomic MinD protein does not regulate plasmid-dependent genes like Pgp5
Autorzy:
Sun, Yina
Kong, Jie
Ma, Jingyue
Qi, Manli
Zhang, Ying
Han, Long
Liu, Quanzhong
Liu, Yuanjun
Powiązania:
https://bibliotekanauki.pl/articles/1038373.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Chlamydia
MinD
Pgp5
Transformation system
Opis:
Chlamydia has a unique intracellular developmental cycle, which has hindered the single protein function study of Chlamydia. Recently developed transformation system of Chlamydia has greatly advanced the chlamydial protein's function research and was used to find that a chlamydial plasmid-encoded Pgp5 protein can down-regulate plasmid-dependent genes. It is assumed, that chlamydial genomic MinD protein has a similar function to Pgp5. However, it is unknown whether MinD protein regulates the same plasmid-dependent genes. We replaced pgp5 gene in the shuttle vector pGFP::CM with minD gene of C. trachomatis (CT0582) or C. muridarum (TC0871). The recombinant plasmid was transformed into plasmid-free organisms-CMUT3 and qRT-PCR was used to detect the transcription level of plasmid-encoded and -dependent genes in these pgp5 deficient organisms. As a readout, GlgA, one of the plasmid-regulated gene products was detected by immunofluorescence assay. After recombination, transformation and plaque purification, the stable transformants CT0582R and TC0871R were generated. In these transformants, the plasmid-dependent genes were up-regulated, alike in the pgp5 premature stop mutant and pgp5 replacement with mCherry mutant. GlgA protein level was also increased in all pgp5 mutants, including CT0582R and TC0871R. Thus, our study showed that genomic MinD protein had different function than Pgp5, which was useful for further understanding the chlamydiae.
Źródło:
Acta Biochimica Polonica; 2018, 65, 3; 425-429
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
SCREENING THE SPECIFIC SUBSTRATES OF ADENYLATION DOMAIN FROM MARINE ACTINOMYCETES BY FLUORESCENCE QUENCHING AND ISOTHERMAL TITRATION CALORIMETRY
Autorzy:
LEI, MING
ZHAO, ZHIJUAN
LIU, KE
LIU, YANLI
ZHANG, HUI
WU, XUE
GONG, SIYING
MA, YANLING
ZHAO, HAOBIN
LIU, JINLIN
MIN, JINRONG
QI, CHAO
Powiązania:
https://bibliotekanauki.pl/articles/895432.pdf
Data publikacji:
2018-12-31
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
A domain
Aspartic acid
Fluorescence quenching (FQ)
Isothermal titration calorimetry (ITC)
Marine actinomycetes
static quenching
Opis:
Adenylation domain (A domain) is a model of non-ribosomal peptide synthetases (NRPs), responsible for binding and activating the substrates-amino acids. In this study, a pGEX-2T-sare0718 recombinant plasmid (the gene sare0718 was cloned from Salinispora arenicola CNS-205, S.arenicola CNS-205) was transformed and expressed as a protein GST-Sare0718. Fluorescence quenching (FQ) was conducted to investigate the binding of 20 common amino acids to GST-Sare0718, then isothermal titration calorimetry (ITC) also be used[changed]. The results of FQ revealed that intrinsic fluorescence of GST-Sare0718 is quenched steadily by addition of aspartic acid (Asp) and glutamine (Gln) through static quenching mechanism. The binding constants Ka are Asp (1.504×106 L/mol) ≥ Gln (1.468×105 L/mol). And there is one binding site on the protein GST-Sare0718. We confirmed Asp preference of GST-Sare0718 by ITC. Therefore, Asp is the specific substrate. In addition, the experimental data indicates that the prediction system, “the specificity-conferring code”, is not suitable for marine actinomycetes. So it is urgent to set up a special predictive system for marine actinomycetes.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 6; 1287-1292
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adsorption of lanthanum(III) and yttrium(III) on kaolinite: kinetics and adsorption isotherms
Autorzy:
Zhou, Fang
Feng, Jian
Xie, Xiong
Wu, Baihong
Liu, Qi
Wu, Xiaoyan
Chi, Ruan
Powiązania:
https://bibliotekanauki.pl/articles/109951.pdf
Data publikacji:
2019
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
adsorption
kaolinite
rare earth
kinetics
Opis:
Experimental investigations were carried out using kaolinite to adsorb two rare earth ions, lanthanum ion (La3+) and yttrium ion (Y3+), which will provide some useful information and new insights on the mineralization process and fractionation phenomenon of weathered crust elution-deposited rare earth ores. The results showed that the equilibrium adsorption capacity of Y3+ is greater than La3+ under the same experimental conditions. The adsorption of rare earth ions presents strongly temperature dependent indicating an endothermic adsorption process. The pseudo-first-order kinetic model and the pseudo-second-order kinetic model were applied to discuss the adsorption kinetics. It was found that the adsorption rate of rare earth follows the pseudo-second-order kinetic model among the adsorption temperature range. Furthermore, the adsorption process of rare earth ions on kaolinite followed the Langmuir isotherm model confirmed by the correlation of experimental equilibrium data to standard isotherm model, Langmuir and Freundlich isotherms. The activation energies for the adsorption of La3+ and Y3+ on kaolinite are 28.1903 kJ/mol and 25.4190 kJ/mol, respectively. All kaolinite before and after adsorption were characterized by XRD and SEM-EDX to understand the adsorption mechanism. The obtained results suggested that the adsorption of La3+ and Y3+ on kaolinite is an endothermic and chemisorption process.
Źródło:
Physicochemical Problems of Mineral Processing; 2019, 55, 4; 928-939
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 33

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