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Wyświetlanie 1-15 z 33
Tytuł:
Self-catalysed hydrogenation of heavy oil and coal mixtures
Autorzy:
Li, Suan
Sun, Zhenguang
Liu, Qi
Ye, Hang
Wang, Kunpeng
Powiązania:
https://bibliotekanauki.pl/articles/27315628.pdf
Data publikacji:
2023
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
self-catalysed
heavy oil
hydrogenation
coal liquefaction
co-processing technology
Opis:
Coal liquefaction and heavy oil processing have become the urgent need for national energy strategic technology reserves in China. However, the inactivation of solid catalysts in these processes is an inevitable problem. Therefore,a self-catalysed method was proposed. The properties of raw oil could be changed by adding a modifier, as it has the function of self-catalysis, and the additional catalyst is no longer needed. The effect of 200 ppm modifier onthe hydrogenation of heavy oil and 500 ppm on the hydrogenation of coal and oil were investigated. The results showed that modifiers could be miscible with heavy oil at 50~100 °C and could change the properties of oil. When the temperature exceeded 250 °C, the sulfur element in the heavy oil combined with the metal element broughtin by the modifier to form a particle with the size of 2–8 nm, which could interact with the hydrogen molecule toactivate the hydrogen molecule. Activated hydrogen atoms further formed the complexes with nickel, vanadium,calcium, iron, and other elements in heavy oil to achieve the purpose of purifying and lightening the oil phase.Therefore, the self-catalysed method could be widely used in oil re fining and would greatly promote the development of the oil refining and catalysis industry.
Źródło:
Polish Journal of Chemical Technology; 2023, 25, 2; 8--14
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation of the co-processing technology of crude oil and coal and its deployment
Autorzy:
Li, Suan
Liu, Qi
Deng, Qingyu
Ye, Hang
Zhou, Xuejie
Qiao, Aijun
Powiązania:
https://bibliotekanauki.pl/articles/2174828.pdf
Data publikacji:
2022
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
crude oil
coal
the co-processing technology
process development
industrial application
Opis:
The effect of process conditions on the co-processing technology of crude oil and coal was investigated. Crude oil/coal matching performance, swelling degree, crude oil/coal slurry viscosity-temperature characteristics and process parameters were obtained via the laboratory scale and pilot scale studies. The optimum reaction temperature of the co-processing was 445~450 °C, the pressure was 19 MPa, the catalyst addition was 3 wt.%, the reaction time was 2 h, and the ratio of hydrogen to crude oil was 1500 (V/V). Furthermore, the co-processing technology including catalyst and corresponding equipment based on the slurry bed hydrogenation were developed. By using this co-processing technology, the feed ratio of crude oil and coal can be 1:1, the coal conversion rate can be over 99%, the light oil (oil and aromatics) yield was over 70%, and the end products were gasoline, diesel, jet fuel, aromatics and LPG. The product quality meets the Euro V standard, whilst aromatics accounted for 48% of the light oil. So it was proved to be feasible to co-refine crude oil and coal at a ratio of 1:1. What’s more, the slurry bed hydrogenation plant and its equipment were tested for long-term operation, and it has been proved that this co-processing technology could be deployed as large-scale industrial application.
Źródło:
Polish Journal of Chemical Technology; 2022, 24, 4; 39--50
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of montmorillonite hydration and delamination on coal flotation
Autorzy:
Zhang, Lucheng
Zhang, Mingqing
Liu, Qi
Song, Shuoshuo
Tian, Yu
Barvora, Joshua Bosco
Powiązania:
https://bibliotekanauki.pl/articles/2175432.pdf
Data publikacji:
2022
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
coal flotation
montmorillonite
hydration
delamination
slime coating
Opis:
In this study, the influence of montmorillonite (MT) hydration and delamination on coal flotation was investigated through flotation tests using coal-MT mixtures. MT particles were subjected to hydration at different time intervals. The Fuerstenau upgrading curve was plotted to evaluate the change in overall flotation selectivity. The zeta potential and particle size distribution were used to characterize the delamination behavior of MT in deionized water at natural pH level. Atomic force microscopy (AFM) (colloidal probe) was used to analyze the interaction force between coal and MT particles. It was found that smaller particles (individual silicate layers or thin packets of layers) with higher zeta potentials appeared gradually, and their volume proportion increased with increasing hydration time. AFM results showed that a monotonous repulsive force was detected consistently throughout the separation distance between coal and these emerging smaller MT particles. The decrease of these MT coating on coal surface was responsible for the higher flotation recovery and better selectivity. A jump-into-contact phenomenon was observed in coal and MT interaction when MT hydrated incompletely. It showed that heterocoagulation between coal and MT occurred and MT coating on the coal surface was responsible for the depression of flotation.
Źródło:
Physicochemical Problems of Mineral Processing; 2022, 58, 6; art. no. 153085
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study of a Compton backscattering wall defects detection device using the Monte Carlo method
Autorzy:
Qin, Xuan
Yang, Jianbo
Du, Zhengcong
Xu, Jie
Li, Rui
Li, Hui
Liu, Qi
Powiązania:
https://bibliotekanauki.pl/articles/24202748.pdf
Data publikacji:
2023
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
compton backscattering
Monte Carlo
nondestructive testing
wall defect
Opis:
In view of the shortcomings of traditional wall defect detection methods, such as small detection range, poor accuracy, non-portable device, and so on, a wall defects detection device based on Compton backscattering technology is designed by Monte Carlo method, which is mainly used to detect the size and location information of defects in concrete walls. It mainly consists of two parts, the source container and the detection system: first, through the simulation and analysis of the parameters such as the receiving angle of thebackscattered particles and the rear collimating material of the detector, the influence of the fluorescent X-ray peak of the detector collimating material on the backscattered particle counts is eliminated and the detected error is reduced; second, the ring array detector design, compared with single array detector and surface array detector, can facilitate real-time detection of defect orientation, expanding the single scan range and improving the detection efficiency. After simulation and comparative analysis, the relevant optimal parameters are obtained: the object is detected using a Cs-137 γ-ray source with an activity of 6 mCi, and a ring detector consisting of four 0.5-inch cube-shaped CsI scintillator detectors is placed at 150° to receive the backscattered photons. The simulation analysis using the Monte Carlo FLUKA program showed that the maximum depth of wall defect detection is 8 cm, the maximum error fl uctuation range of defect depth and thickness is ±1 cm, the overall device weight is <20 kg, and the measurement time is <5 min.
Źródło:
Nukleonika; 2023, 68, 2; 57--63
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adsorption of lanthanum(III) and yttrium(III) on kaolinite: kinetics and adsorption isotherms
Autorzy:
Zhou, Fang
Feng, Jian
Xie, Xiong
Wu, Baihong
Liu, Qi
Wu, Xiaoyan
Chi, Ruan
Powiązania:
https://bibliotekanauki.pl/articles/109951.pdf
Data publikacji:
2019
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
adsorption
kaolinite
rare earth
kinetics
Opis:
Experimental investigations were carried out using kaolinite to adsorb two rare earth ions, lanthanum ion (La3+) and yttrium ion (Y3+), which will provide some useful information and new insights on the mineralization process and fractionation phenomenon of weathered crust elution-deposited rare earth ores. The results showed that the equilibrium adsorption capacity of Y3+ is greater than La3+ under the same experimental conditions. The adsorption of rare earth ions presents strongly temperature dependent indicating an endothermic adsorption process. The pseudo-first-order kinetic model and the pseudo-second-order kinetic model were applied to discuss the adsorption kinetics. It was found that the adsorption rate of rare earth follows the pseudo-second-order kinetic model among the adsorption temperature range. Furthermore, the adsorption process of rare earth ions on kaolinite followed the Langmuir isotherm model confirmed by the correlation of experimental equilibrium data to standard isotherm model, Langmuir and Freundlich isotherms. The activation energies for the adsorption of La3+ and Y3+ on kaolinite are 28.1903 kJ/mol and 25.4190 kJ/mol, respectively. All kaolinite before and after adsorption were characterized by XRD and SEM-EDX to understand the adsorption mechanism. The obtained results suggested that the adsorption of La3+ and Y3+ on kaolinite is an endothermic and chemisorption process.
Źródło:
Physicochemical Problems of Mineral Processing; 2019, 55, 4; 928-939
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Looped-chain-based active current sharing strategy in DC microgrids
Autorzy:
Li, D.
Liu, Y.
Gu, Y.
Qi, H.
Powiązania:
https://bibliotekanauki.pl/articles/141109.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
active current sharing
DC microgrids
looped control diagram
Opis:
The integration of renewable energy sources in modern electric grids have drawn increasing attention nowadays. In order to effectively manage large-scale renewable energy sources and achieve flexible and efficient operation, the concept of microgrids have been proposed. Considering the nature of DC outputs in many distributed energy resources (DERs), DC microgrids have been extensively studied in the past years. Among the operational issues in DC microgrids, current sharing issues have become an important topic since it is highly relevant to the operation of DC microgrids. By adopting a proper design of current sharing strategy in DC microgrids, the current rating violations in each interface converter can be successfully avoided. In this paper, a looped-chainbased active current sharing strategy is proposed to realize high accuracy current sharing in DC microgrids. In particular, the output current is shared between the neighboring interface converters. Hence, following a clockwise or counter-clockwise order, a loopedchain- based control diagram can be established to share the reference value of the output current. A final status in the whole DC microgrid is that the output current of every interface converter is equalized. Hence, the desired current sharing objective can be satisfied. A MATLAB simulation model is established to verify the proposed loopedchain- based active current sharing strategy in DC microgrids.
Źródło:
Archives of Electrical Engineering; 2017, 66, 3; 559-569
1427-4221
2300-2506
Pojawia się w:
Archives of Electrical Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Laser Welding of TA15 Titanium Alloy and Inconel 718 Dissimilar Metals
Autorzy:
An, Qi
Wu, Dongting
Liu, Peng
Zou, Yong
Powiązania:
https://bibliotekanauki.pl/articles/2174556.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
TA15 titanium alloy
Inconel 718 nickel-based alloy
laser welding
microstructure
tensile strength and fracture characteristic
Opis:
This paper studied the effect of laser welding technology on dissimilar metal welding joints of TA15 titanium alloy and Inconel 718 nickel-based alloy. The research results indicate that the laser welding of TA15 titanium alloy and Inconel 718 nickel-based alloy directly was difficult to form well, which due to the intermetallic compounds caused the joint brittle. When the pure Cu foil was used as the filling layer, the quality of the welding joints can be improved effectively. The experimental results also indicate that there were brittle intermetallic-compounds in the laser welding seam, and the laser power had an important influence on the performance and mechanical properties of the dissimilar metal joint. The maximum average tensile strength of the welding joint of 2300 W was increased to 252.32 MPa. Scanning electron microscope(SEM) results show that the fracture morphology was river pattern, a typical morphological of cleavage fracture, and the mode was brittle fracture.
Źródło:
Archives of Metallurgy and Materials; 2022, 67, 4; 1267--1276
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The presence of Chlamydia phage PhiCPG1 capsid protein VP1 genes and antibodies in patients infected with Chlamydia trachomatis
Autorzy:
Ma, Jingyue
Liu, Yuan
Liu, Yuanjun
Li, Lingjie
Hou, Shuping
Gao, Xibo
Qi, Manli
Liu, Quanzhong
Powiązania:
https://bibliotekanauki.pl/articles/1038770.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Chlamydia trachomatis
Chlamydia bacteriophage
capsid protein
VP1
Opis:
Chlamydia phage PhiCPG1 has been found in Chlamydia caviae in a guinea pig model for inclusion conjunctivitis, raising the possibility that Chlamydia phage is also present in patients infected with C. trachomatis (Ct). In the present study, we assayed for presence of Chlamydia phage capsid protein VP1 genes and antibodies in 84 non-Ct controls and 206 Ct patients using an enzyme-linked immunoassay (ELISA), followed by verification with Western blot. None of the subjects were exposed to an antibiotic treatment or had a C. pneumoniae infection. The VP1 antibody test was positive in both, the ELISA and Western blot assay, in 4 Ct patients. PCR amplification experiments revealed presence of the VP1 gene in 5 Ct patients. The results suggest that Chlamydia phage capsid protein VP1 may exist in some Ct patients.
Źródło:
Acta Biochimica Polonica; 2016, 63, 3; 501-504
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of Metal Oxides on the Thermal Decomposition Kinetics and Mechanisms of HAN/PVA Based Propellants
Autorzy:
Hu, Song-qi
Liu, Xue-li
Liu, Lin-Lin
Kang, Bo
Zhang, Yan
Powiązania:
https://bibliotekanauki.pl/articles/27787991.pdf
Data publikacji:
2021
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
HAN/PVA-based propellant
metal oxides
catalytic behaviour
kinetic parameters
Opis:
The thermal decomposition processes of HAN/PVA-based propellants have been investigated using a simultaneous thermogravimetric analysis (TGA) – differential scanning calorimetry (DSC), coupled with Fourier-Transform Infrared Spectroscopy (FTIR) and Mass Spectrometry (MS) system. The activation energy (Ea), pre-exponential factor A and reaction mechanism function f(α) of the decomposition processes have been determined by non-isothermal and Malek methods. The results showed that the decomposition process of an HAN/PVA sample occurs mainly in the temperature range 202.2~220.1 °C, with a mass loss, heat release and Ea of about 84.8%, 1474.18 and 88.76 kJ·mol–1, respectively. Of the seven metal oxides studied as catalysts, Al2O3, V2O5 and Fe2O3 have significant catalytic effects on an HAN/PVA-based propellant, in lowering the decomposition temperature, with Ea changing from 88.8 to 83.7, 85.6 and 113.6 kJ·mol–1, respectively. The f(α) of both HAN/PVA and HAN/PVA/Al2O3 samples can be expressed as f(α) = (1 – α)2, whereas f(α) = α or f(α) = α/2 fit well for the HAN/PVA/V2O5 and HAN/PVA/Fe2O3 samples.
Źródło:
Central European Journal of Energetic Materials; 2021, 18, 3; 322-338
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on Friction and Wear Characteristics of Aluminum Alloy Hydraulic Valve Body and Its Antiwear Mechanism
Autorzy:
Rong, L.
Lunjun, Ch.
Ming, Su
Qi, Z.
Yong, Liu
Powiązania:
https://bibliotekanauki.pl/articles/382468.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
aluminum alloy
hydraulic valve body
oil leakage
friction and wear
stop aluminium
korpus zaworu hydraulicznego
wycieki oleju
tarcie i zużycie
Opis:
In order for the working status of the aluminum alloyed hydraulic valve body to be controlled in actual conditions, a new friction and wear design device was designed for the cast iron and aluminum alloyed valve bodies comparison under the same conditions. The results displayed that: (1) The oil leakage of the aluminum alloyed hydraulic valve body was higher than the corresponding oil leakage of the iron body during the initial running stage. Besides during a later running stage, the oil leakage of the aluminum alloyed body was lower than corresponding oil leakage of the iron body; (2) The actual oil leakage of different materials consisted of two parts: the foundation leakage that was the leakage of the valve without wear and wear leakage that was caused by the worn valve body; (3) The aluminum alloyed valve could rely on the dust filling furrow and melting mechanism that led the body surface to retain dynamic balance, resulting in the valve leakage preservation at a low level. The aluminum alloy modified valve body can meet the requirements of hydraulic leakage under pressure, possibly constituting this alloy suitable for hydraulic valve body manufacturing.
Źródło:
Archives of Foundry Engineering; 2017, 17, 1; 207-215
1897-3310
2299-2944
Pojawia się w:
Archives of Foundry Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mixed leaf litter decomposition and N, P release with a focus on Phyllostachys edulis (Carriere) J. Houz. forest in subtropical southeastern China
Autorzy:
Shi, L.
Fan, S.
Jiang, Z.
Qi, L.
Liu, G.
Powiązania:
https://bibliotekanauki.pl/articles/56692.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
leaf litter
decomposition
nitrogen
phosphorus
Phyllostachys edulis
moso bamboo
tortoise-shell bamboo zob.moso bamboo
forest
subtropical area
China
Opis:
As an important non-wood forest product and wood substitute, Moso bamboo grows extremely rapidly and hence acquires large quantities of nutrients from the soil. With regard to litter decomposition, N and P release in Moso bamboo forests is undoubtedly important; however, to date, no comprehensive analysis has been conducted. Here, we chose two dominant species (i.e., Cunninghamia lanceolata and Phoebe bournei), in addition to Moso bamboo, which are widely distributed in subtropical southeastern China, and created five leaf litter mixtures (PE100, PE80PB20, PE80CL20, PE50PB50 and PE50CL50) to investigate species effects on leaf litter decomposition and nutrient release (N and P) via the litterbag method. Over a one-year incubation experiment, mass loss varied significantly with litter type (P < 0.05). The litter mixtures containing the higher proportions (>80%) of Moso bamboo decomposed faster; the remaining litter compositions followed Olson's decay mode well (R2 > 0.94, P < 0.001). N and P had different patterns of release; overall, N showed great temporal variation, while P was released from the litter continually. The mixture of Moso bamboo and Phoebe bournei (PE80PB20 and PE50PB50) showed significantly faster P release compared to the other three types, but there was no significant difference in N release. Litter decomposition and P release were related to initial litter C/N ratio, C/P ratio, and/or C content, while no significant relationship between N release and initial stoichiometric ratios was found. The Moso bamboo-Phoebe bournei (i.e., bamboo-broadleaved) mixture appeared to be the best choice for nutrient return and thus productivity and maintenance of Moso bamboo in this region.
Źródło:
Acta Societatis Botanicorum Poloniae; 2015, 84, 2
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Intelligent fault diagnosis of rolling bearings based on continuous wavelet transform-multiscale feature fusion and improved channel attention mechanism
Autorzy:
Zhang, Jiqiang
Kong, Xiangwei
Cheng, Liu
Qi, Haochen
Yu, Mingzhu
Powiązania:
https://bibliotekanauki.pl/articles/24200817.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
deep learning
continuous wavelet transform
improved channel attention mechanism
multi-conditions
convolutional neural network
Opis:
Accurate fault diagnosis is critical to operating rotating machinery safely and efficiently. Traditional fault information description methods rely on experts to extract statistical features, which inevitably leads to the problem of information loss. As a result, this paper proposes an intelligent fault diagnosis of rolling bearings based on a continuous wavelet transform(CWT)-multiscale feature fusion and an improved channel attention mechanism. Different from traditional CNNs, CWT can convert the 1-D signals into 2-D images, and extract the wavelet power spectrum, which is conducive to model recognition. In this case, the multiscale feature fusion was implemented by the parallel 2-D convolutional neural networks to accomplish deeper feature fusion. Meanwhile, the channel attention mechanism is improved by converting from compressed to extended ways in the excitation block to better obtain the evaluation score of the channel. The proposed model has been validated using two bearing datasets, and the results show that it has excellent accuracy compared to existing methods.
Źródło:
Eksploatacja i Niezawodność; 2023, 25, 1; art. no. 16
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Estimation of wind erosion rates of Mongolian Plateau by using 137Cs tracing technique
Ocena intensywnosci erozji eolicznej Plaskowyzu Mongolskiego z wykorzystaniem techniki oznaczania 137Cs
Autorzy:
Qi, Y
Liu, J.
Shi, H.
Zhuang, D.
Hu, Y.
Powiązania:
https://bibliotekanauki.pl/articles/81677.pdf
Data publikacji:
2010
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
estimation
wind erosion
erosion rate
Mongolian Plateau
137Cs tracing technique
Opis:
Wind erosion is one of the major environmental problems in semi-arid and arid regions. Here we established a transect from northwest (Tariat, Mongolia) to southeast (Xilingol, Inner Mongolia of China) across the Mongolian Plateau, and selected eight sampling sites along the transect. We then estimated the soil wind erosion rates by using the 137Cs tracing technique and examined their spatial dynamics. In the Mongolia section (from Tariat to Sainshand), the wind erosion rate increased gradually with vegetation type and climatic regimes; the wind erosion process was controlled by physical factors such as annual precipitation and vegetation coverage, etc. While in the China section (Inner Mongolia), the wind erosion rates of Xilinhot, Zhengxiangbai Banner and Taipusi Banner were thrice as much as those of Bayannur of Mongolia, although these four sites were all dominated by typical steppe. Besides the physical factors, higher population density and livestock carrying level should be responsible for the higher wind erosion rates in these regions of Inner Mongolia.
W pracy przedstawiono wyniki badań intensywności erozji eolicznej Płaskowyżu Mongolskiego, określonej na podstawie ośmiu prób gruntu pobranego wzdłuż transektu o długości około 1400 km, przebiegającego przez teren Mongolii i Chin. Do oceny intensywności erozji wykorzystano technikę oznaczania 137Cs w wierzchnich warstwach gruntu do głębokości 30 cm. Stwierdzono, że intensywność erozji w analizowanym transekcie od północnego-zachodu stopniowo wzrasta wraz ze zmianami reżimu klimatycznego (zmniejszania się rocznej wysokości opadów), a także zmianami pokrycia roślinnego. Chińska część Płaskowyżu o charakterze stepowym jest trzykrotnie bardziej intensywnie erodowana. Oprócz czynników fizycznych, na intensywność procesu erozji eolicznej wpływa większa gęstość zaludnienia Płaskowyżu na terenie Chin, a także większe pogłowie zwierząt hodowlanych.
Źródło:
Annals of Warsaw University of Life Sciences - SGGW. Land Reclamation; 2010, 42, 1; 197-208
0208-5771
Pojawia się w:
Annals of Warsaw University of Life Sciences - SGGW. Land Reclamation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chlamydial genomic MinD protein does not regulate plasmid-dependent genes like Pgp5
Autorzy:
Sun, Yina
Kong, Jie
Ma, Jingyue
Qi, Manli
Zhang, Ying
Han, Long
Liu, Quanzhong
Liu, Yuanjun
Powiązania:
https://bibliotekanauki.pl/articles/1038373.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Chlamydia
MinD
Pgp5
Transformation system
Opis:
Chlamydia has a unique intracellular developmental cycle, which has hindered the single protein function study of Chlamydia. Recently developed transformation system of Chlamydia has greatly advanced the chlamydial protein's function research and was used to find that a chlamydial plasmid-encoded Pgp5 protein can down-regulate plasmid-dependent genes. It is assumed, that chlamydial genomic MinD protein has a similar function to Pgp5. However, it is unknown whether MinD protein regulates the same plasmid-dependent genes. We replaced pgp5 gene in the shuttle vector pGFP::CM with minD gene of C. trachomatis (CT0582) or C. muridarum (TC0871). The recombinant plasmid was transformed into plasmid-free organisms-CMUT3 and qRT-PCR was used to detect the transcription level of plasmid-encoded and -dependent genes in these pgp5 deficient organisms. As a readout, GlgA, one of the plasmid-regulated gene products was detected by immunofluorescence assay. After recombination, transformation and plaque purification, the stable transformants CT0582R and TC0871R were generated. In these transformants, the plasmid-dependent genes were up-regulated, alike in the pgp5 premature stop mutant and pgp5 replacement with mCherry mutant. GlgA protein level was also increased in all pgp5 mutants, including CT0582R and TC0871R. Thus, our study showed that genomic MinD protein had different function than Pgp5, which was useful for further understanding the chlamydiae.
Źródło:
Acta Biochimica Polonica; 2018, 65, 3; 425-429
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
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