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Wyświetlanie 1-15 z 103
Tytuł:
Review and future perspectives on the integration characteristics for equine lentivirus in the host genome
Autorzy:
Yu, Y.-Y.
Xu, M.-S.
Liang, H.
Wang, H.-Y.
Yu, C.-Q.
Liu, Q.
Powiązania:
https://bibliotekanauki.pl/articles/16647532.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
lentivirus
equine infectious anemia virus
integration
long interspersed elements
Opis:
Despite over 40 years of research on the human immunodeficiency virus type 1 (HIV-1) vaccine, we still lack a considerable progress. Equine infectious anemia virus (EIAV) is a lentivirus in the Retroviridae family, akin to HIV-1 in genome structure and antigenicity. EIA is an important infectious disease in equids, characterized by anemia, persistent infection, and repeated fevers. The EIAV attenuated vaccine in China is the only lentiviral vaccine used on a large scale. Elucidating the mechanism of waning and induction of protective immunity from this attenuated vaccine strain will provide a critical theoretical basis and reference point for vaccine research, particularly in the development of lentivirus vaccines, with far-reaching scientific value and social significance. In this paper, we summarize the information related to EIAV integration site selection, particularly for the Chinese EIAV attenuated vaccine strains on the equine genome. This may improve our mechanistic understanding of EIAV virulence reduction at the host genome level. The obtained data may help elucidate the biological characteristics of EIAV, particularly the Chinese attenuated EIAV vaccine strain, and provide valuable information regarding retroviral infections, particularly lentiviral infection and associated therapeutic vectors.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 1; 163-172
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative analysis of CpG islands in two genotypes of African swine fever virus
Autorzy:
Yu, Y.-Y.
X, M.-S.
Liu, Q.
Powiązania:
https://bibliotekanauki.pl/articles/16539329.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
African Swine Fever Virus (ASFV)
genotype
DNA methylation
CpG island
Opis:
African swine fever (ASF) is an acute, hemorrhagic, and devastating viral infectious disease that causes important economic losses to the swine industry. Currently, there are no effective vaccines or drugs available. Epigenetic mechanisms, especially cytosine methylation of cytosine-phosphate-guanine (CpG) islands, have a significant impact on the life cycle of several viruses. Hence, drugs targeting DNA methylation may potentially be used for the treatment of ASF. Here, we selected the inner core, core shell, inner membrane, capsid, and external envelope membrane, to analyze the characteristics of CpG islands in the ASF virus (ASFV) genomes. Furthermore, we analyzed the promoters and CpG islands in the upstream regions of these genes. Results showed that the CpG islands of seven genes were conserved in the genomes of two genotype of ASFV strains, whereas the CpG islands of other genes were relatively conserved (ASFV strains differed mainly in the quantity of CpG islands). The different distribution of CpG islands in the genomes of different ASFV strains may affect their methylation status, which may in turn affect the regulation of viral gene expression, leading to different clinical outcomes. In addition, the predicted promoter regions based on the upstream sequences of most genes overlapped with CpG island positions. Methylation of the binding sites of the promoter regions inhibits the binding of the transcription factors to the promoters, thus inhibiting the activation of the promoters and limiting the synthesis of viral proteins. The results of this study provide a basis for exploring new antiviral therapeutic strategies from an epigenetic perspective.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 3; 455-462
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) in various types of cells in thymic tissues
Autorzy:
Liu, Q.
Yu, Y.-Y.
Wang, H.-Y.
Powiązania:
https://bibliotekanauki.pl/articles/16539177.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
pig
thymus
highly pathogenic porcine reproductive and respiratory syndromevirus
immunofluorescence double labeling method
target cells
Opis:
Thymus, an important central immune organ in pigs, is the site of T lymphocyte development and maturation and an important target organ for infection and replication of various pathogens. Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection results in severe thymic atrophy in piglets. This study aimed to explore the effects of HP-PRRSV on the thymic structure of piglets to elucidate the pathogenesis of thymic atrophy induced by HP-PRRSV. In this study, histopathological techniques and immunofluorescence double staining techniques were used to analyze thymic tissues infected by HP-PRRSV to explore the structural changes of thymus caused by the viral infection and its target cell types. An antibody of cluster of differentiation (CD) 3 (CD3), CD20, CD80, or calgranulin + calprotectin was applied to identify T cells, B cells, dendritic cells (DCs), and macrophages, respectively. The results indicated that a variety of cell components in the thymic tissue were diffusely damaged after viral infection. In the infected thymic tissue, CD80- or calgranulin + calprotectin- -labeled cells supported the HP-PRRSV infection, whereas CD3-labeled T cells and CD20- -labeled B cells did not support the viral infection. The results showed that HP-PRRSV caused the reduction of visible cell components in the thymic tissue, and the virus attacked CD80- and calgranulin + calprotectin-positive cells (such as DCs and macrophages) in the thymic tissue, which played an important role in the pathogenesis of thymus atrophy. These results lay the foundation for elucidating the immunosuppression of piglets after infection with HP-PRRSV.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 2; 287-294
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Interferon-γ-mediated gene expression in porcine alveolar macrophage; An in vitro model
Autorzy:
Liu, Q.
Wang, H.-Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087220.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pig
alveolar macrophage
IFN-γ
transcriptomic analysis
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 135-143
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Bending, Impact Fracture Behavior and Characteristics of Stainless Steel Clad Plates with Different Rolling Temperature
Autorzy:
An, Q.
Fan, K. Y.
Ge, Y. F.
Liu, B. X.
He, J.
Wang, S.
Chen, C. X.
Ji, P. G.
Tolochko, O.
Powiązania:
https://bibliotekanauki.pl/articles/2049433.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
stainless steel clad plate
interface characteristics
bending behavior
impact morphologies
delamination crack
Opis:
The interface characteristics, bending and impact behavior, as well as fracture characteristics of stainless steel clad plates fabricated by vacuum hot rolling at different rolling temperatures of 1100°C, 1200°C and 1300°C are investigated in detail. The interface bonding strength is gradually increased with the increasing rolling temperature due to the sufficient diffusion behavior of alloy element. The bending toughness and impact toughness are gradually decreased, while the bending strength increase with the increase of the rolling temperature, which is attributed to mechanisms of matrix softening and interface strengthening at high rolling temperature. Due to the weak interface at 1100°C, the bending and impact crack propagation path was displaced by delamination cracks, which in turn lead to reduction in stress intensity of the main crack, playing an effective role in toughening the stainless steel clad plates. Moreover, the impact fracture morphologies of clad plates show a typical ductile-brittle transition phenomenon, which is attributed to the matrix softening behavior with the increasing rolling temperature.
Źródło:
Archives of Metallurgy and Materials; 2021, 66, 1; 229-239
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genome-wide association study of terpenoids in resin reveals candidate genes for resin yield in Pinus massoniana
Autorzy:
Bai, Q.
Zhang, Q.
Cai, Y.
Lian, H.
Liu, W.
Luo, M.
Zeng, L.
He, B.
Powiązania:
https://bibliotekanauki.pl/articles/2078149.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Pinus massoniana
GC-MS
terpenoid
GWAS
candidate gene
Opis:
Masson pine (Pinus massoniana) is an important species for resin tapping in China. Molecular assisted selection (MAS) has been gradually introduced in to the selection and breeding of masson pine. Until now, the lack of connection between genome information and phenotypic data has hindered the breeding and selection of important chemical compounds of xylem resin. In this study, gas chromatography mass spectrometer (GC-MS) was employed for qualitative and quantitative analysis of chemical compounds of resin collected from 69 masson pine germplasm resources. Then, genome-wide association study (GWAS) was conducted for the terpenoids with higher heritability. A total of 15 primary compounds, including six diterpenes and nine monoterpenes/sesquiterpenes, were detected from xylem resin. By using five GWAS methods, 42 associated SNPs were identified from these masson pines. The P. taeda genome database was used for detecting candidate genes related to the associated SNPs. By using transcriptome data, several members of PKc_like super family, Cytochrome P450, AP2-ERF, F-box, and ABC transporter were found to be highly expressed in the masson pines with higher resin-yielding capacity. The terpenoids associated SNPs can be directly used for MAS. These candidate genes, especially the two F-box members, will contribute to the study of terpenoid biosynthesis mechanisms in the future.
Źródło:
Dendrobiology; 2020, 84; 109-121
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Response of dry matter translocation and grain yield of summer maize to biodegradable film in the North China Plain
Autorzy:
Tan, D.
Guo, L.
Liu, J.
Fan, Y.
Li, Q.
Powiązania:
https://bibliotekanauki.pl/articles/2082862.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
polyethylene film
leaf area index
dry matter
transfer
yield components
North China Plain
Opis:
In the North China Plain, it is a matter of urgency to explore the feasibility of using biodegradable film to replace polyethylene film. A field experiment was conducted by covering soils with polyethylene white film, biodegradable white film, biodegradable black film, while the control remained uncovered. This study analysed the effects of using different film types on summer maize dry matter accumulation and transfer, grain yield and yield components during the 2016 and 2017 summer maize growing seasons. Results showed that, for both growing seasons, compared with non-mulching, dry matter translocation, dry matter transfer efficiency of vegetative organs and grain yield for plants following polyethylene white film and biodegradable white film treatments were always lower. However, dry matter accumulation, dry matter translocation, dry matter transfer efficiency, grain yield, and the contribution of dry matter translocation to grain yield before flowering in biodegradable black film treatments significantly increased by 21.0, 33.3, 21.4, 12.6, and 18.5%, respectively. Only the black biodegradable film could increase grain yield as determined by the 1000 kernel mass. Results indicate that black biodegradable films are a viable alternative to polyethylene film in summer maize production in the North China Plain.
Źródło:
International Agrophysics; 2020, 34, 1; 87-94
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seismic damage behaviour of aeolian sand reinforced concrete columns with I-shaped structural steel
Autorzy:
Wang, Y. H.
Wang, H. Y.
Wang, Y. P.
Zhao, M.
Qi, J.
Huo, G. Z.
Liu, P. Q.
Powiązania:
https://bibliotekanauki.pl/articles/1849772.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
piasek eoliczny
słup żelbetowy
stal konstrukcyjna
przekrój I
zachowanie
uszkodzenie sejsmiczne
model uszkodzenia
aeolian sand
column
I-shaped steel
structural steel
seismic damage
behaviour
damage model
Opis:
To promote the application of aeolian sand resources for steel-concrete composite structures, an aeolian sand reinforced concrete column with I-shaped structural steel is proposed in this study. Four specimens are designed and manufactured with different replacement rates of aeolian sand. The seismic behaviour and damage evolution process of the specimens are studied by low-cycle repeated loading tests. Based on the test results, the mechanical characteristics, failure modes, hysteresis curves, skeleton curves, energy dissipation capacity, displacement ductility, and stiffness degradation of the specimens with different replacement rates of aeolian sand are analysed. In addition, the effects of the design parameters on the seismic behaviour of the specimens are also studied. The results show that the indexes of the seismic behaviour can be significantly improved by adding steel. Moreover, a revised damage model is proposed, to better reflect the evolution law of seismic damage of aeolian sand reinforced concrete columns with steel. The proposed model can provide an important reference for seismic damage assessment of the columns.
Źródło:
Archives of Civil Engineering; 2020, 66, 4; 633-650
1230-2945
Pojawia się w:
Archives of Civil Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An expert elicitation analysis for vessel allision risk near the offshore wind farm by using fuzzy rule-based bayesian network
Autorzy:
Yu, Q.
Liu, K.
Powiązania:
https://bibliotekanauki.pl/articles/117215.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
offshore wind farms
expert elicitation analysis
Bayesian network model
fuzzy rule-based bayesian network
Failure Modes and Effects Analyses (FMEA)
Bayesian Networks
vessel allision risk
risk analysis
Opis:
This paper develops an expert based framework for analysing and synthesising the ship allision risk near the offshore wind farm (OWF) on the basis of a generic Fuzzy Bayesian network and FMEA analysis. This framework is specifically intended to overcome the difficulty of using traditional risk assessment methods in OWF allision. Under the introduced framework, subjective belief degrees are assigned to model the incompleteness encountered in establishing the knowledge base. The fuzzy transformation technology is then used to introduce all judgements results under various situations. Fully, a Bayesian network is established to aggregate all relevant attributes to the conclusion and to prioritise potential allision risk level of each ship categories. A series of case studies of different ship categories are studied to illustrate the application of the proposed framework. Results show that the fishing vessel and the service vessel have a higher allision risk than the merchant vessel due to insufficient risk detection. The collision consequence of the tanker is significantly higher than other types of vessel. The framework facilitates subjective risk assessment when historical failure data is not available in their practice, which provides support to OWF-safeguarding and decision-making.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2019, 13, 4; 831-837
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphological homology, evolution, and proposed nomenclature for bear dentition
Autorzy:
Jiangzuo, Q.
Liu, J.
Chen, J.
Powiązania:
https://bibliotekanauki.pl/articles/21119.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Opis:
Bears are a group of carnivores with diverse diets and complicated dental structure. Several large rearrangements of dental structures are known in different lineages of bears, making the homology of dental structures between the different bears difficult to evaluate. By tracing the evolutionary processes of the two lineages of bears with the most complicated dental structure, i.e., the giant panda lineage (Ailuropodinae) and cave bear lineage (Ursinae), we were able to clarify the homology of dental structures of the two subfamilies. We define a new assemblage of dental nomenclature (based mainly on the homology to the giant panda) that can be very useful to infer the evolution of fossil bears. The evolutionary positions of some fossil bears are reviewed based on our results.
Źródło:
Acta Palaeontologica Polonica; 2019, 64, 4
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A continuous-time distributed algorithm for solving a class of decomposable nonconvex quadratic programming
Autorzy:
Zhao, Y.
Liu, Q.
Powiązania:
https://bibliotekanauki.pl/articles/91832.pdf
Data publikacji:
2018
Wydawca:
Społeczna Akademia Nauk w Łodzi. Polskie Towarzystwo Sieci Neuronowych
Tematy:
decomposable nonconvex quadratic programming
multi-agent network
consensus
Lyapunov method
Opis:
In this paper, a continuous-time distributed algorithm is presented to solve a class of decomposable quadratic programming problems. In the quadratic programming, even if the objective function is nonconvex, the algorithm can still perform well under an extra condition combining with the objective, constraint and coupling matrices. Inspired by recent advances in distributed optimization, the proposed continuous-time algorithm described by multi-agent network with consensus is designed and analyzed. In the network, each agent only accesses the local information of its own and from its neighbors, then all the agents in a connected network cooperatively find the optimal solution with consensus.
Źródło:
Journal of Artificial Intelligence and Soft Computing Research; 2018, 8, 4; 283-291
2083-2567
2449-6499
Pojawia się w:
Journal of Artificial Intelligence and Soft Computing Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A method for tracing exogenous DNA uptake in live spermatozoa and embryos
Autorzy:
Mu, Y.
Wang, J.
Liu, Z.
Zhao, Y.
Zhang, X.
Jiao, M.
Lv, J.
Hao, J.
Kong, Q.
Powiązania:
https://bibliotekanauki.pl/articles/2087751.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
spermatozoa
SMGT
exogenous DNA
IVF
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 193-202
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An improved feature extraction method for rolling bearing fault diagnosis based on MEMD and PE
Autorzy:
Zhang, H.
Zhao, L.
Liu, Q.
Luo, J.
Wei, Q.
Zhou, Z.
Qu, Y.
Powiązania:
https://bibliotekanauki.pl/articles/259770.pdf
Data publikacji:
2018
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
improved feature extraction method
rolling bearing fault diagnosis
MEMD
PE
Opis:
The health condition of rolling bearing can directly influence to the efficiency and lifecycle of rotating machinery, thus monitoring and diagnosing the faults of rolling bearing is of great importance. Unfortunately, vibration signals of rolling bearing are usually overwhelmed by external noise, so the fault frequencies of rolling bearing cannot be readily obtained. In this paper, an improved feature extraction method called IMFs_PE, which combines the multivariate empirical mode decomposition with the permutation entropy, is proposed to extract fault frequencies from the noisy bearing vibration signals. First, the raw bearing vibration signals are filtered by an optimal band-pass filter determined by SK to remove the irrelative noise which is not in the same frequency band of fault frequencies. Then the filtered signals are processed by the IMFs_PE to get rid of the relative noise which is in the same frequency band of fault frequencies. Finally, a frequency domain condition indicator FFR(Fault Frequency Ratio), which measures the magnitude of fault frequencies in frequency domain, is calculated to compare the effectiveness of the feature extraction methods. The feature extraction method proposed in this paper has advantages of removing both irrelative noise and relative noise over other feature extraction methods. The effectiveness of the proposed method is validated by simulated and experimental bearing signals. And the results are shown that the proposed method outperforms other state of the art algorithms with regards to fault feature extraction of rolling bearing.
Źródło:
Polish Maritime Research; 2018, S 2; 98-106
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Calculation Method for the Bandgap of Antimonide Based Multicomponent Alloys
Autorzy:
An, N.
Liu, C.
Fan, C.
Dong, X.
Song, Q.
Powiązania:
https://bibliotekanauki.pl/articles/1030612.pdf
Data publikacji:
2018-01
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
81.05.Ea
42.55.Px
81.15.Hi
Opis:
As the most important material parameter of semiconductor, bandgap is necessary to be investigated to meet the design requirements of the high-performance optoelectronic devices. A new method of is proposed to calibrate the bandgap of antimonide based multi-component alloys with considering the effect of spin-orbit splitting off bands and the doublet degeneracy of valance band on the bandgaps of Sb-containing materials. A correction factor is introduced in the conventional calculation, and the spin-orbit splitting method is proposed. Besides, the In_xGa_{1-x}As_ySb_{1-y} films with different compositions are grown on GaSb substrates by molecular beam epitaxy, and the corresponding bandgaps are obtained by photoluminescence to test the accuracy and reliability of this new method. An error rate analysis reveals that the α calculated by the spin-orbit splitting correction method is decreased to 2%, almost one order of magnitude smaller than the Moon method, which means that the new method can calculate the antimonide multicomponent more accurately with some applicability. This work can give a reasonable interpretation for the reported results and beneficial to tailor the antimonides properties and optoelectronic devices.
Źródło:
Acta Physica Polonica A; 2018, 133, 1; 118-120
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 103

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