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Tytuł:
A novel reliability estimation method of multi-state system based on structure learning algorithm
Nowatorska metoda oceny niezawodności systemów wielostanowych w oparciu o algorytm uczenia struktury
Autorzy:
Li, Zhifeng
Wang, Zili
Ren, Yi
Yang, Dezhen
Lv, Xing
Powiązania:
https://bibliotekanauki.pl/articles/301718.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
reliability analysis
Bayesian network
structure learning
multi-state system (MSS)
dependent failure
analiza niezawodności
sieć bayesowska
uczenie struktury
system wielostanowy
uszkodzenie zależne
Opis:
Traditional reliability models, such as fault tree analysis (FTA) and reliability block diagram (RBD), are typically constructed with reference to the function principle graph that is produced by system engineers, which requires substantial time and effort. In addition, the quality and correctness of the models depend on the ability and experience of the engineers and the models are difficult to verify. With the development of data acquisition, data mining and system modeling techniques, the operational data of a complex system considering multi-state, dependent behavior can be obtained and analyzed automatically. In this paper, we present a method that is based on the K2 algorithm for establishing a Bayesian network (BN) for estimating the reliability of a multi-state system with dependent behavior. Facilitated by BN tools, the reliability modeling and the reliability estimation can be conducted automatically. An illustrative example is used to demonstrate the performance of the method.
Tradycyjne modele niezawodności, takie jak analiza drzewa błędów (FTA) czy schemat blokowy niezawodności (RBD), buduje się zazwyczaj w oparciu o tworzone przez inżynierów systemowych schematy zasad działania systemu, których przygotowanie wymaga dużych nakładów czasu i pracy. Jakość i poprawność tych modeli zależy od umiejętności i doświadczenia inżynierów, a same modele są trudne do zweryfikowania. Dzięki rozwojowi technik akwizycji i eksploracji danych oraz modelowania systemów, dane operacyjne złożonego systemu uwzględniające jego zależne, wielostanowe zachowania mogą być pozyskiwane i analizowane automatycznie. W artykule przedstawiono metodę konstrukcji sieci bayesowskiej (BN) opartą na algorytmie K2, która pozwala na ocenę niezawodności systemu wielostanowego o zachowaniach zależnych. Dzięki narzędziom BN, modelowanie i szacowanie niezawodności może odbywać się automatycznie. Działanie omawianej metody zilustrowano na podstawie przykładu.
Źródło:
Eksploatacja i Niezawodność; 2020, 22, 1; 170-178
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Abruptly autofocusing property of circular Pearcey vortex beams with different initial launch angles in harmonic potentials
Autorzy:
Liu, Xianglian
Zhang, Di
Zhao, Lijiao
Li, Pu
Zhang, Jianguo
Liu, Yi
Powiązania:
https://bibliotekanauki.pl/articles/2172824.pdf
Data publikacji:
2022
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
autofocusing beam
optical vortices
Pearcey
Opis:
We have studied and explored the influence of different launch angles on the circular Pearcey beams (CPBs) without vortex or with vortex for the first time. Although launch angles can manipulate the focal length and the contrast of peak intensity of the CPBs, the shape and propagation trajectory of the CPBs maintain invariant. When the vortex is considered, the focal pattern and the contrast of peak intensity of the circular Pearcey vortex beams (CPVBs) can be changed by adjusting the magnitude of topological charges and the position of vortex. In addition, we have deliberated the propagation of the CPVBs under the action of double opposite optical vortices.
Źródło:
Optica Applicata; 2022, 52, 3; 417--428
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An effective hybrid method for analysis the large-scale reliability block diagram model
Autorzy:
Ping, Yashi
Ren, Yi
Li, Zhifeng
Yang, Dezhen
Yang, Chao
Powiązania:
https://bibliotekanauki.pl/articles/27312772.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
reliability block diagram
structure identification
plus structure
binary decision diagram
Opis:
The reliability block diagram (RBD) is a graphical tool used for reliability modeling and analysis in various industries, including shipbuilding, aviation, and aerospace. Typically, RBDs are transformed into Bayesian networks for quantitative analysis of systems. Bayesian networks are probabilistic graphical models that can capture the uncertainties and causal relationships in complex systems. They can provide various reliability metrics such as failure probability, mean time to failure, availability, etc. However, these techniques have several drawbacks, especially for large-scale models, such as being extremely time and memory-consuming. To address these issues, we propose a hybrid method for quantitative analysis of large-scale RBDs based on the structure identification approach and binary decision diagrams. Theoretical analysis and case verification demonstrate that the proposed method is significantly more efficient than the current one.
Źródło:
Eksploatacja i Niezawodność; 2023, 25, 3; art. no. 169408
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analytical solution for one-dimensional consolidation of unsaturated soils under dynamic load
Autorzy:
Liu, Zhi-Yi
Song, Yu
Zhou, Feng-Xi
Wang, Li-Ye
Powiązania:
https://bibliotekanauki.pl/articles/2200882.pdf
Data publikacji:
2023
Wydawca:
Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
Tematy:
unsaturated soil
consolidation characteristic
dynamic load
analytical solution
numerical calculation
Opis:
Firstly, in this paper, based on the theory of the porous elastic medium and combined with the effective stress principle of unsaturated soil, a set of governing equations is established to describe consolidation of the unsaturated soil. Secondly, an analytical expression under any dynamic loads is obtained with the help of Laplace integral transformation. Finally, analysis of numerical examples under specific boundary conditions is made to discuss one-dimensional consolidation characteristics under harmonic loads and the influence of factors on the consolidation characteristics of unsaturated soil, such as excitation frequency and initial saturation.
Źródło:
Journal of Theoretical and Applied Mechanics; 2023, 61, 1; 11--22
1429-2955
Pojawia się w:
Journal of Theoretical and Applied Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of response surface methodology to improve methane production from jerusalem artichoke straw
Autorzy:
Meng, Yan
Li, Yi
Chen, Laisheng
Han, Rui
Powiązania:
https://bibliotekanauki.pl/articles/2203117.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Jerusalem artichoke straw
anaerobic digestion
response surface methodology
methane production
topinambur
słonecznik bulwiasty
fermentacja beztlenowa
produkcja metanu
RSM
Opis:
Anaerobic digestion (AD) converts organic matter and biomass waste into biogas, making it an environmentally friendly technology to improve energy resources for a wide range of applications. Jerusalem artichoke straw (JAS) has an enriched content of cellulose and exhibits a high potential for methane production. AD-based production of methane can eff ectively utilize waste JAS. This study investigated the AD performance of JAS to explore the enhancement of methane yields by employing a Box-Behnken experimental design (BBD) of response surface methodology (RSM). The overall goal was to identify the optimal levels of pretreatment factors, including HCl concentration, pretreatment time, and pretreatment temperature, for producing optimal biomethane yields from JAS. The highest value of methane production achieved was 256.33 mL g-1VS by using an optimal concentration of HCl as 0.25 M, a pretreatment time of 10 h, and a pretreatment temperature of 25°C. These results inform the future application of JAS in enhanced methane production.
Źródło:
Archives of Environmental Protection; 2022, 48, 3; 70--79
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Average capacity analysis of FSO system with Airy beam as carrier over exponentiated Weibull channels
Autorzy:
Li, Yi
Chu, Xingchun
Han, Zhongxiang
Tang, Hanling
Song, Xinkang
Powiązania:
https://bibliotekanauki.pl/articles/2202763.pdf
Data publikacji:
2023
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
Airy beam
free-space optical communication
channel capacity
exponentiated Weibull model
Opis:
Based on scintillation index of Airy beam and exponentiated Weibull channel model, analytical expressions of average channel capacity for free-space optical (FSO) communication links with Airy beam as signal carrier under weak atmospheric turbulence and on-off keying modulation scheme are derived. The average capacity at various propagation distances, transverse scale factors and exponential decay factors has been evaluated. And we compared the average capacity of FSO links with Airy beam and Gaussian beam as signal carrier. The results show that the average capacity of FSO links with Airy beam as carrier increases with the increase of mean signal-to-noise ratio and decreases uniformly with the increase of propagation distance. When the transverse scale factor of Airy beam is about 2 cm, a higher average capacity can be obtained. And the smaller the exponential decay factor of Airy beam, the larger the average capacity. Under the same source power or source width, the average capacity of FSO links with Airy beam as carrier is significantly higher than that of FSO links with Gaussian beam as carrier. The results of this research have some reference significance for the application of Airy beam in FSO communication system.
Źródło:
Optica Applicata; 2023, 53, 1; 35--48
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biochemical characterization of a catalase from Vibrio vulnificus, a pathogen that causes gastroenteritis
Autorzy:
Pei, Jihua
Wang, Haijun
Wu, Limin
Xia, Shenglong
Xu, Changlong
Zheng, Bo
Li, Tianya
Jiang, Yi
Powiązania:
https://bibliotekanauki.pl/articles/1038620.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
catalase
Vibrio vulnificus
kinetics
mutation
gastroenteritis.
Opis:
Vibrio vulnificus is a virulent human pathogen causing gastroenteritis and possibly life threatening septicemia in patients. Most V. vulnificus are catalase positive and can deactivate peroxides, thus allowing them to survive within the host. In the study presented here, a catalase from V. vulnificus (CAT-Vv) was purified to homogeneity after expression in Escherichia coli. The kinetics and function of CAT-Vv were examined. CAT-Vv catalyzed the reduction of H2O2 at an optimal pH of 7.5 and temperature of 35°C. The Vmax and Km values were 65.8±1.2 U/mg and 10.5±0.7 mM for H2O2, respectively. Mutational analysis suggests that amino acids involved in heme binding play a key role in the catalysis. Quantitative reverse transcription-PCR revealed that in V. vulnificus, transcription of CAT-Vv was upregulated by low salinity, heat, and oxidative stresses. This research gives new clues to help inhibit the growth of, and infection by V. vulnificus.
Źródło:
Acta Biochimica Polonica; 2017, 64, 3; 543-549
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI.
Autorzy:
Zhang, Jiayi
Dai, Jianliang
Zhao, Enpeng
Lin, Yun
Zeng, Li
Chen, Jinzhong
Zheng, Huari
Wang, Yu
Li, Xin
Ying, Kang
Xie, Yi
Mao, YuMin
Powiązania:
https://bibliotekanauki.pl/articles/1041522.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
ovary
ePAD
peptidylarginine deiminase
hPADVI
Opis:
Peptidylarginine deiminase (PAD) catalyzes the post-translational modification of protein through the conversion of arginine to citrulline in the presence of calcium ions. Human, similar to rodents, has four isoforms of PAD (type I, II, III and IV/V), each of which is distinct in substrate specificity and tissue specific expression. In our large-scale sequencing project, we identified a new human PAD cDNA from a human fetal brain cDNA library. The putative protein encoded by this cDNA is designated hPADVI. Expression analysis of hPADVI showed that it is mainly expressed in adult human ovary and peripheral blood leukocytes. We conclude that hPADVI may be orthologous to mouse ePAD, basing on sequence comparison, chromosome localization and exon-intron structure analysis. PAD-mediated deimination of epithelial cell keratin resulting in cytoskeletal remodeling suggests a possible role for hPADVI in cytoskeletal reorganization in the egg and in early embryo development. This study describes a new important member of the human PAD family.
Źródło:
Acta Biochimica Polonica; 2004, 51, 4; 1051-1058
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chemical Footprint of the Wet Processing of Cotton Fabric
Ślad chemiczny mokrej obróbki tkanin bawełnianych
Autorzy:
Qian, Weiran
Qiu, Xiaoxiao
Guo, Yiqi
Ji, Xiang
Li, Yi
Wang, Laili
Powiązania:
https://bibliotekanauki.pl/articles/1857664.pdf
Data publikacji:
2021
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
Tematy:
chemical footprint
USEtox
human toxicity
ecotoxicity
grey target decision-making model
ślad chemiczny
toksyczność
ekotoksyczność
model podejmowania decyzji
Opis:
The chemical footprint (ChF) can identify the harmful effects of discharged chemical pollutants, helping producers to select environmentally friendly chemicals to reduce their negative environmental impact. This paper quantified and evaluated the ChF of the wet processing of cotton fabric with data collected from a dyeing enterprise. The results showed that the discharged sodium hydroxide caused the most severe impact in terms of both human toxicity and ecotoxicity due to the extensive usage and its high toxicity. The discharged sodium carbonate and dimethyl silicone oil also had a greater environmental impact. Comprehensive evaluation of human toxicity and ecotoxicity with a multi-objective grey target decision-making model indicated that the pretreatment process had the most significant impact, followed by the finishing process and dyeing process. More attention should be paid to the pretreatment process, such as the selection of environmentally friendly textile chemicals, in order to reduce the native impacts of the wet processing of cotton fabric.
Ślad chemiczny (ChF) może identyfikować szkodliwe skutki emitowanych zanieczyszczeń chemicznych, pomagając producentom wybrać przyjazne dla środowiska środki chemiczne w celu zmniejszenia ich negatywnego wpływu na środowisko. W artykule określono ilościowo i oceniono ChF mokrej obróbki tkaniny bawełnianej na podstawie danych zebranych z przedsiębiorstwa farbiarskiego. Wyniki pokazały, że odprowadzany wodorotlenek sodu miał najpoważniejszy wpływ zarówno pod względem toksyczności dla człowieka, jak i ekotoksyczności, ze względu na szerokie zastosowanie i wysoką toksyczność. Odprowadzany węglan sodu i dimetylosilikonowy olej również miały duży wpływ na środowisko. Kompleksowa ocena toksyczności dla ludzi i ekotoksyczności z zastosowaniem wielocelowego modelu podejmowania decyzji wykazała, że największy wpływ miał proces obróbki wstępnej, a następnie proces wykańczania i proces barwienia. Wskazane jest zwrócenie większej uwagi na proces obróbki wstępnej, w szczególności na wybór przyjaznych dla środowiska chemikaliów tekstylnych.
Źródło:
Fibres & Textiles in Eastern Europe; 2021, 4 (148); 100-104
1230-3666
2300-7354
Pojawia się w:
Fibres & Textiles in Eastern Europe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Color image encryption scheme based on quaternion discrete multi-fractional random transform and compressive sensing
Autorzy:
Ye, Huo-Sheng
Dai, Jing-Yi
Wen, Shun-Xi
Gong, Li-Hua
Zhang, Wen-Quan
Powiązania:
https://bibliotekanauki.pl/articles/2033985.pdf
Data publikacji:
2021
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
color image encryption
quaternion discrete multi-fractional random transform
compressive sensing
confusion-diffusion strategy
Opis:
A color image compression-encryption algorithm by combining quaternion discrete multi-fractional random transform with compressive sensing is investigated, in which the chaos-based fractional orders greatly improve key sensitivity. The original color image is compressed and encrypted with the assistance of compressive sensing, in which the partial Hadamard matrix adopted as a measurement matrix is constructed by iterating Chebyshev map instead of utilizing the entire Guassian matrix as a key. The sparse images are divided into 12 sub-images and then represented as three quaternion signals, which are modulated by the quaternion discrete multi-fractional random transform. The image blocking and the quaternion representation make the proposed cryptosystem avoid additional data extension existing in many transform-based methods. To further improve the level of security, the plaintext-related key streams generated by the 2D logistic-sine-coupling map are adopted to diffuse and confuse the intermediate results simultaneously. Consequently, the final ciphertext image is attained. Simulation results reveal that the proposed cryptosystem is feasible with high security and has strong robustness against various attacks.
Źródło:
Optica Applicata; 2021, 51, 3; 349-364
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Particle Sizes of Slag on Reduction Characteristics of Nickel Slag-Coal Composite Briquette
Autorzy:
Li, Xiaoming
Li, Yi
Xing, Xiangdong
Wang, Yanjun
Wen, Zhenyu
Yang, Haibo
Powiązania:
https://bibliotekanauki.pl/articles/2049420.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
resources reutilization
nickel slag
particle size
direct reduction
reduction characteristics
Opis:
Nickel slag has a high-content iron and is a secondary utilization resource with great development potential. The coal-based direct reduction is an innovative technology that can be used to utilize the iron resources in nickel slag. The effect of the particle size of nickel slag on the strength and the reduction of nickel slag-coal composite briquettes were investigated. Four samples with particle size of 75~106 μm, 106~150 μm, 150~270 μm, and >270 μm were selected. The drop strength increased 9.4 times and the compressive strength reached 281.1 N when the nickel slag particle size decreased from >270 μm to 75~106 μm. The reduction degree determined by the data from the thermogravimetric experiment indicated that its maximum was 79.545%. The reduction experiments performed at 1200°C for 45 minutes indicated that the nickel slag with particle sizes between 75~106 μm were appropriate for the reduction of the nickel slag-coal composite briquettes.
Źródło:
Archives of Metallurgy and Materials; 2021, 66, 1; 127-134
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of a water hammer and cavitation on vibration transients in a reservoir-pipe-valve system
Autorzy:
Miao, Yang
Qiu, Zaihui
Zhang, Xiaolu
Jiang, Yuncheng
Pan, Jun
Liu, Yi
Zhang, Li
Li, Kun
Powiązania:
https://bibliotekanauki.pl/articles/2055065.pdf
Data publikacji:
2021
Wydawca:
Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
Tematy:
reservoir-pipe-valve system (RPV system)
water hammer
vibration
cavitation
Opis:
An extraordinary phenomenon with violent oscillation, accompanied by an abnormal “click” sound is observed during the valve closing in a fuel feeding pipe system. A fluctuation model with flow cavitation, in which time-varying stiffness, time-varying damping coefficients and flow cavitation are comprehensively considered, is proposed. On this basis, a dynamic vibration equation is established and an expression of flow pressure at the valve port is derived. The critical displacement of the flow cavitation system is defined. When the vibration amplitude reaches the critical displacement, cavitation occurs. Based on this model, simulation of vibration displacement and flow pressure is given. The simulated transient process shows the phenomena of vibration fluctuation with cavitation. The results are compared with the experimental data measured by a pressure sensor. Under our experimental conditions, the critical displacement of vibration is 1.41mm, and the average relative pressure peak error is 0.022. The comparison between the simulation and experimental results shows that they are in an acceptable agreement.
Źródło:
Journal of Theoretical and Applied Mechanics; 2021, 59, 4; 611--622
1429-2955
Pojawia się w:
Journal of Theoretical and Applied Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Facile synthesis and characterization of zinc tetranitro phthalocyanine-MWCNTs nanocomposites with efficient visible-light-driven photocatalytic activity
Autorzy:
Wan, Yi
Chen, Shuang
Wang, Guanqiu
Liang, Qian
Li, Zhongyu
Xu, Song
Powiązania:
https://bibliotekanauki.pl/articles/1158623.pdf
Data publikacji:
2016-09
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
78.67.Bf
81.05.Zx
81.07.Pr
Opis:
Novel zinc tetranitro-phthalocyanine (ZnTNPc) supported by multi-walled carbon nanotubes hybrid composites were facilely prepared by a method of ultrasonic impregnation and their photocatalysis behavior was studied. The as-prepared ZnTNPc-MWCNTs composites were characterized by scanning electron microscopy, X-ray diffraction, diffuse reflectance spectra, transmission electron microscopy, thermogravimetric analysis, and the Fourier transform infrared spectra. The results showed that the ZnTNPc was not only grown on the multi-walled carbon nanotubes but also uniformly disturbed without aggregation. Compared with pure ZnTNPc and MWCNTs, ZnTNPc-MWCNTs nanocomposites presented a significantly enhanced photocatalytic activity for the degradation of rhodamine B under visible-light irradiation. Furthermore, a possible mechanism for the photodegradation of rhodamine B was also proposed.
Źródło:
Acta Physica Polonica A; 2016, 130, 3; 785-790
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Human neutrophil peptide 3 could be functionally expressed in Rhodobacter sphaeroides
Autorzy:
Nie, Xin
Zhang, Li
Hu, Zongli
Liu, Yi
Zhao, Zhiping
Powiązania:
https://bibliotekanauki.pl/articles/1039101.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Rb. sphaeroides
HNP3
expression system
antimicrobial activity
spectral absorption
Opis:
Human neutrophil peptides (HNPs) possess high antimicrobial activities against a broad spectrum of microorganisms. Rhodobacter sphaeroides is the best-characterized photosynthetic bacterium and exhibits potential as a novel expression system. Up to date, no literature has been reported regarding expression of HNP3 in Rb. sphaeroides. In the present study, the HNP3 gene fragment was amplified by SOE PCR and ligated into photosynthetic bacteria light-harvesting complex 2 (LH2) expression vector leading to HNP3 fusion protein expression vector. The HNP3 fusion protein was successfully expressed as rapidly evaluated by the LH2 characteristic peaks at ~800 nm and ~850 nm before purification and SDS/PAGE. Subsequently, the HNP3 fusion protein was purified by one-step affinity chromatography, and could be rapidly detected by the color and the spectral absorption at ~800 nm and ~850 nm before SDS/PAGE. Antimicrobial activity assay suggested that the HNP3 fusion protein exhibited high antimicrobial activity towards E. coli. The present study may supply an insight into employing the novel Rb. sphaeroides expression system, exhibiting dramatic advantages over currently used commercial expression system, to heterologously express human neutrophil peptides.
Źródło:
Acta Biochimica Polonica; 2015, 62, 2; 259-263
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Non-histone nuclear protein HMGN2 differently regulates the urothelium barrier function by altering expression of antimicrobial peptides and tight junction protein genes in UPEC J96-infected bladder epithelial cell monolayer
Autorzy:
Tian, Hanwen
Miao, Junming
Zhang, Fumei
Xiong, Feng
Zhu, Feimei
Li, Jinyu
Wang, Xiaoying
Chen, Shanzhe
Chen, Junli
Huang, Ning
Wang, Yi
Powiązania:
https://bibliotekanauki.pl/articles/1038529.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
HMGN2
UPEC
BECs 5637
bladder epithelium
tight junction
antimicrobial peptides
Opis:
The urinary tract is vulnerable to frequent challenges from environmental microflora. Uropathogenic Escherichia coli (UPEC) makes a major contribution to urinary tract infection (UTI). Previous studies have characterized positive roles of non-histone nuclear protein HMGN2 in lung epithelial innate immune response. In the study presented here, we found HMGN2 expression was up-regulated in UPEC J96-infected urothelium. Surprisingly, over-expression of HMGN2 promoted disruption of BECs 5637 cells' intercellular junctions by down-regulating tight junction (TJs) components' expression and physical structure under J96 infection. Further investigation showed that BECs 5637 monolayer, in which HMGN2 was over-expressed, had significantly increased permeability to J96. Our study systemically explored the regulatory roles of HMGN2 in BECs barrier function during UPEC infection and suggested different modulations of intracellular and paracellular routes through which UPEC invades the bladder epithelium.
Źródło:
Acta Biochimica Polonica; 2018, 65, 1; 93-100
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł

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