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Tytuł:
Delay-dependent generalized H2 control for discrete T-S fuzzy large-scale stochastic systems with mixed delays
Autorzy:
Li, J.
Xia, Z.
Powiązania:
https://bibliotekanauki.pl/articles/930162.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
system stochastyczny
opóźnienie zależne
liniowa nierówność macierzowa
fuzzy large scale stochastic system
delay-dependent
generalized H2 control
infinite distributed delays
linear matrix inequality
Opis:
This paper is concerned with the problem of stochastic stability and generalized H2 control for discrete-time fuzzy largescale stochastic systems with time-varying and infinite-distributed delays. Large-scale interconnected systems consist of a number of discrete-time interconnected Takagi-Sugeno (T-S) subsystems. First, a novel Delay-Dependent Piecewise Lyapunov-Krasovskii Functional (DDPLKF0 is proposed, in which both the upper and the lower bound of delays are considered. Then, two improved delay-dependent stability conditions are established based on this DDPLKF in terms of Linear Matrix Inequalities (LMIs). The merit of the proposed conditions lies in its reduced conservatism, which is achieved by circumventing the utilization of some bounding inequalities for cross products of two vectors and by considering the interactions among the fuzzy subsystems in each subregion. A decentralized generalized H2 state feedback fuzzy controller is designed for each subsystem. It is shown that the mean-square stability for discrete T-S fuzzy large-scale stochastic systems can be established if a DDPLKF can be constructed and a decentralized controller can be obtained by solving a set of LMIs. Finally, an illustrative example is provided to demonstrate the effectiveness of the proposed method.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2011, 21, 4; 585-603
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effects of velvet antler polypeptides on the phenotype and related biological indicators of osteoarthritic rabbit chondrocytes
Autorzy:
Zhang, Zhengyao
Liu, Xiaofeng
Duan, Lengxin
Li, Xia
Zhang, Yang
Zhou, Qiuli
Powiązania:
https://bibliotekanauki.pl/articles/1039876.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
velvet antler polypeptides
MMP
osteoarthritic chondrocytes
apoptosis
Opis:
Objective: To study the effects of velvet antler polypeptides (VAPs) on osteoarthritic chondrocytes (OCs) in rabbits. Methods: An osteoarthritic rabbit model was established according to Hulth's method. OCs were isolated and cultured for observation of the cell cycle. Cell proliferation was detected by MTT assay and the cell cycle was monitored by flow cytometry. The phenotype was determined by toluidine blue staining as well as immunohistochemical staining for collagen type II. The expression of MMP-1, MMP-3, MMP-13, TIMP-1, and collagen I and X mRNA by chondrocytes was assayed by RT-PCR. Results: The VAPs had no obvious proliferative effect on OCs and did not affect the cell cycle. However, they significantly reduced the proportion of early apoptotic cells in a dose-dependent manner. Further, VAPs inhibited the expression of collagen I and X mRNA and induced abnormal expression of MMP-1 and MMP-13 mRNA. VAPs had no significant effect on MMP-3 and TIMP-1 mRNA levels. The toluidine blue and collagen type II immunohistochemical staining intensities of VAP-treated chondrocytes were positively correlated with the concentration of VAPs used. Conclusion: VAPs had no significant effect on OC proliferation and the cell cycle, but did increase the glycosaminoglycan (GAG) and collagen type II expression levels in the extracellular matrix, and down-regulated collagen I and X mRNA expression. Treatment of cartilage cells with VAPs maintained their normal phenotype, inhibited matrix metalloproteinases (MMPs) secretion, kept the balance of cartilage matrix metabolism, and sustained an external environment where the cartilage cells could survive. Moreover, VAPs reduced the proportion of early apoptotic cells, suggesting that they may block the apoptotic pathway in OCs.
Źródło:
Acta Biochimica Polonica; 2011, 58, 3; 297-302
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers
Autorzy:
Wu, Fen
Liu, Jing
Qiu, Yu-Lan
Wang, Wei
Zhu, Shou-Min
Sun, Pin
Miao, Wen-Bin
Li, Yong-Liang
Brandt-Rauf, Paul W.
Xia, Zhao-Lin
Powiązania:
https://bibliotekanauki.pl/articles/2179802.pdf
Data publikacji:
2013-03-01
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
vinyl chloride monomer
chromosome damage
MGMT
hMLH1
DNA methylation
Opis:
Objective: To explore the association of the methylation status of MGMT and hMLH1 with chromosome damage induced by vinyl chloride monomer (VCM). Materials and Methods: Methylation of MGMT and hMLH1 was measured in 101 VCM-exposed workers by methylation-specifi c PCR. Chromosome damage in peripheral blood lymphocytes was measured by the cytokinesis-block micronucleus assay. The subjects were divided into chromosome damaged and non-damaged groups based on the normal reference value of micronuclei frequencies determined for two control groups. Results: MGMT promoter methylation was detectable in 5 out of 49 chromosome damaged subjects, but not in the chromosome non-damaged subjects; there was a signifi cant difference in MGMT methylation between the two groups (p < 0.05). Conclusions: We detected aberrant promoter methylation of MGMT in a small number of chromosome damaged VCM-exposed workers, but not in the chromosome non-damaged subjects. This preliminary observation warrants further investigation in a larger study.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2013, 26, 1; 173-182
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polymorphisms in the p53 pathway genes and micronucleus occurrence in Chinese vinyl chloride-exposed workers
Autorzy:
Li, Yong
Feng, Nan-Nan
Zhang, Guang-Hui
Wang, Qi
Hao, Yan-Hui
Nanzhang, Ya
Long, Changxu
Li, Yongliang
Brandt-Rauf, Paul W.
Xia, Zhao-Lin
Powiązania:
https://bibliotekanauki.pl/articles/2179059.pdf
Data publikacji:
2013-12-01
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
VC
occupational exposure
p53 pathway genes
genetic polymorphism
Opis:
Objectives: To investigate the association between polymorphisms in the p53 pathway genes and chromosomal damage in vinyl chloride (VC)-exposed workers. Materials and Methods: Cytokinesis block micronucleus test was performed in 310 VC-exposed workers and 149 non-exposed workers to determine chromosomal damage. The polymerase chain reaction and restriction fragment length polymorphism technique were used to detect six SNPs in the p53 pathway genes involved in the cell cycle. Results: There was a highly significant dose-response relationship between VC exposure and chromosomal damage. Individuals carrying the variant genotypes were at higher risk for chromosomal damage compared with their wild type genotype: p53rs1042522, MDM2 Del1518rs3730485, MDM2rs2279744 and GADD45Ars532446. On the other hand, individuals possessing the variant genotype of CDKN2A rs3088440 had significantly decreased risk compared with the corresponding wild-type. Conclusions: Genetic polymorphisms in P53 pathway genes may have an impact on VC-induced chromosomal damage.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2013, 26, 6; 825-836
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Colloidal crystal cladded microfiber for refractive index sensing
Autorzy:
Yan, H. T.
Zhao, X Y
Zhang, Ch.
Zhen, Z Q
Li, Q Z
Cao, J X
Xia, L X
Powiązania:
https://bibliotekanauki.pl/articles/174902.pdf
Data publikacji:
2014
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
microfibers
colloidal crystals
refractive index sensing
Opis:
We investigate the evanescent field of a microfiber wrapped by colloidal crystals. The microfiber has the diameter of about 1 μm that is drawn from a single-mode fiber with an alcohol lamp. The colloidal spheres are further attached to the microfiber through thermal evaporation, then they self-assemble to crystal-like structures. The 400 nm, 590 nm, and 710 nm-diameter SiO2 colloidal spheres are used, respectively. The spectral responses are studied theoretically and experimentally, and the results agree with each other. It is revealed that the evanescent field of a microfiber could be modulated by the photonic band-gap of colloidal crystals. This characteristic is very useful in refractive index sensing for liquids.
Źródło:
Optica Applicata; 2014, 44, 2; 309-315
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Noether Theorems and Discrete Variational Integrators in Field Theory
Autorzy:
Xia, Li-Li
Chen, Li-Qun
Liu, Chang-Xin
Powiązania:
https://bibliotekanauki.pl/articles/1398640.pdf
Data publikacji:
2015-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
02.20.Sv
11.30.-j
Opis:
The discrete analogue of the Noether-type identities in field theory is investigated by means of the difference discrete variational principle in which the difference is regarded as an entire geometric object. The discrete counterparts of the Noether theorems are obtained. It is proved that there exists the discrete version of the Noether conservation law in field theory. The discretization for the nonlinear Schrödinger equation is presented to illustrate the results.
Źródło:
Acta Physica Polonica A; 2015, 127, 3; 669-673
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polarization properties of Gaussian–Schell model quantization field in a turbulent marine-atmosphere
Autorzy:
Zhao, Y.
Xia, M.
Wang, Q.
Li, Y.
Hu, Z.
Sun, H.
Zhang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/173639.pdf
Data publikacji:
2016
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
polarization fluctuation
Gaussian–Schell model
marine-atmosphere
Kolmogorov turbulence
Opis:
Polarization properties of Gaussian–Schell model quantization field propagating through the Kolmogorov turbulence of a marine-atmosphere channel are studied based on the degree of quantum polarization. The effective photon annihilation and creation operators of Gaussian–Schell model quantization field propagation in a marine-atmosphere are developed by making use of the extended Huygens–Fresnel integral of quantum field. The effects of the outer scale on the degree of polarization can be neglected. As the source transverse coherent width, the number of received photons, the inner scale of turbulent eddies, and the source transverse radius decrease or the re- fractive index structure parameter increases, the degree of polarization decreases. In theory, we find that the polarization fade of marine-atmosphere turbulence channels is larger than that of terrene-atmosphere turbulence channels under same transport parameters and the channel with a stronger turbulence strength will possess a larger detection area of a polarization signal, which have potentially important implications for free-space quantum key distribution.
Źródło:
Optica Applicata; 2016, 46, 3; 335-345
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seasonal dynamics in cambial activity and the formation of xylem and phloem in the branches of Cinnamomum camphora
Autorzy:
Dong, M.
Xu, Y.M.
Lin, H.
Li, X.Q.
Xia, Q.Q.
Powiązania:
https://bibliotekanauki.pl/articles/41385.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
seasonal dynamics
cambial activity
formation
xylem
phloem
branch formation
tree
tropical tree
Cinnamomum camphora
wood formation
phenology
Opis:
The cambial activity and periodicity of secondary xylem and phloem formation have been less studied in tropical tree species than in temperate ones. Cambium activity is the only source of timber production. Studies on its activity and xylem formation can provide helpful data for dendrochronology and possible factors that control tree growth, the yield and quality of wood. Cinnamomum camphora is an important fast-growing tree for furniture and sculpture wood in South China. Its dynamics of cambial activity was not reported. During the growth season in 2011, five trees of Cinnamomum camphora plantation were monitored on the campus of Huazhong Agricultural University in central china (located on 113°41’ to 115°05’ E and 29°58’ to 31°22’ N). Sampling time was from 13, February 2011 to 13, February 2012. Some small stems of 15–20 cm length were selected to sample in the middle of 2–3 years old health branches in the central crown of these trees. The observational study found that the cambium of camphor trees was active from March to October and dormant from November to February. Onset of cambial cell division was observed in early March, one week after sprouting of new buds. The morphology of the cells and number of cell layers in the cambium zone varied throughout the year. In early March, cambium reactivated with the rising of temperature, the cell wall was relative thick. The number of cell layers increased from 2–3 in middle Feb. to 3–5. In June, the number of cell layers in cambium reached the maximum of 5–7 in a radial direction. In mid March, phloem cells began to differentiate, followed by xylem three weeks later. The number of cell layers in immature phloem and xylem increased dramatically before mid June and the increment of cell layers in xylem was almost fifth times of phloem in the differentiating process. The phloem cells mainly stopped dividing new cells in end of October, but xylem cells were still in the activity a week later. The annual activities of cambium was in accordance with the air mean temperature change in 2011–2012. The results showed that the cambial activity and formation of xylem and phloem were related to the environmental factors, especially the temperature.
Źródło:
Dendrobiology; 2016, 75
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biochemical characterization of a catalase from Vibrio vulnificus, a pathogen that causes gastroenteritis
Autorzy:
Pei, Jihua
Wang, Haijun
Wu, Limin
Xia, Shenglong
Xu, Changlong
Zheng, Bo
Li, Tianya
Jiang, Yi
Powiązania:
https://bibliotekanauki.pl/articles/1038620.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
catalase
Vibrio vulnificus
kinetics
mutation
gastroenteritis.
Opis:
Vibrio vulnificus is a virulent human pathogen causing gastroenteritis and possibly life threatening septicemia in patients. Most V. vulnificus are catalase positive and can deactivate peroxides, thus allowing them to survive within the host. In the study presented here, a catalase from V. vulnificus (CAT-Vv) was purified to homogeneity after expression in Escherichia coli. The kinetics and function of CAT-Vv were examined. CAT-Vv catalyzed the reduction of H2O2 at an optimal pH of 7.5 and temperature of 35°C. The Vmax and Km values were 65.8±1.2 U/mg and 10.5±0.7 mM for H2O2, respectively. Mutational analysis suggests that amino acids involved in heme binding play a key role in the catalysis. Quantitative reverse transcription-PCR revealed that in V. vulnificus, transcription of CAT-Vv was upregulated by low salinity, heat, and oxidative stresses. This research gives new clues to help inhibit the growth of, and infection by V. vulnificus.
Źródło:
Acta Biochimica Polonica; 2017, 64, 3; 543-549
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Design, preparation and properties of novel flame retardant thermosetting vinyl ester copolymers based on castor oil and industrial dipentene
Autorzy:
Mao, W.
Li, S.
Li, M.
Huang, K.
Xia, J.
Powiązania:
https://bibliotekanauki.pl/articles/777956.pdf
Data publikacji:
2017
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
castor oil
vinyl ester resin
copolymers
flammability
thermal properties
Opis:
A novel bio-based flame-retardant thermosetting vinyl ester resin monomer was synthesized from castor oil. The chemical structures of the monomer was characterized by FTIR and 1H-NMR. In order to improve its rigidity and expand its application in the field of bio-based materials, it was mixed with certain proportions of another reactive bio-based VER monomer, which had rigid and strong polar groups, and then a series of copolymers were prepared with thermal curing method. Then their tensile property, hardness, morphology of fractured surface, flame retardant property, DMA and thermostability were all investigated. The results indicated that the copolymers had relatively high tensile strength of 11.2 MPa, and the limiting oxygen index is above 23% in all prepared copolymers. DMA demonstrates that the glass transition temperature of the cured resins is up to 56.1°C. Thermogravimetric analysis shows that the copolymers have excellent thermal stability.
Źródło:
Polish Journal of Chemical Technology; 2017, 19, 3; 1-8
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of preparation methods of mixed calcium and zinc thermal stabilizers derived from dimer fatty acid and tung-oil based C22 triacid on properties of PVC
Autorzy:
Li, M.
Wang, M.
Li, S.
Huang, K.
Mao, W.
Xia, J.
Powiązania:
https://bibliotekanauki.pl/articles/778123.pdf
Data publikacji:
2017
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
poly(vinyl chloride)
mixed calcium and zinc thermal stabilizer
synthesis technology
thermal stabilization
Opis:
Calcium and zinc salts of dimer fatty acids (DFA-Ca and DFA-Zn) were synthesized using direct neutralization and metathesis technologies, respectively. The adduct of maleic anhydride and methyl eleostearate (MAME) was also converted to the corresponding zinc soap (C22TA-Zn) and calcium soap (C22TA-Ca) by the two different synthetic routes. Mixed Ca/Zn salts between DFA-Ca and DFA-Zn, and between C22TA-Zn and C22TA-Ca were used as thermal stabilizers for poly(vinyl chloride) (PVC). The PVC thermal stability was determined using Congo red test, discoloration test, torque rheological analysis and TGA. Dynamic mechanical properties were also tested. Results indicated that the DFA-Ca/DFA-Zn thermal stabilizer from direct neutralization technology was found to be superior to that of the metathesis product. The C22TA-Ca/C22TA-Zn thermal stabilizer from direct neutralization method had overall superior thermal stability, and displayed modulus and glass transition comparable to that of metathesis product. Direct neutralization method was more excellent and convenient than metathesis technology.
Źródło:
Polish Journal of Chemical Technology; 2017, 19, 2; 78-87
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression and immunological evaluation of elongation factor Tu of Streptococcus suis serotype 2
Autorzy:
Xia, X.J.
Wang, L.
Cheng, L.K.
Shen, Z.Q.
Li, S.G.
Wang, J.L.
Powiązania:
https://bibliotekanauki.pl/articles/31256.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Streptococcus suis serotype 2 (SS2) is considered as a major pathogen that causes sepsis and meningitis in piglets and humans, but knowledge of its antigenic proteins remains limited so far. The surface-related proteins of pathogens often play significant roles in bacterium-host interactions and infection. Here, we obtained the elongation factor Tu (EF-Tu) gene of Streptococcus suis and constructed the recombinant expression plasmid successfully. The target recombinant plasmid was then expressed in Escherichia coli and the immuno-protection of the recombinant protein was subsequently evaluated as well. The EF-Tu gene of Streptococcus suis is 1197 bp in length, encodes 398 amino acids. The target recombinant EF-Tu (rEF-Tu) protein can recognize the antiserum of Streptococcus suis and can provoke obvious humoral immune responses in rabbits and conferred protection to rabbits against Streptococcus suis ear-vein challenge, implying that the EF-Tu may be used as an attractive candidate antigen for a component of subunit vaccine.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Removal of uranium(VI) from aqueous solution by rice husk
Autorzy:
Xia, L.
Li, R.
Xiao, Y.
Zheng, W.
Tan, K.
Powiązania:
https://bibliotekanauki.pl/articles/208201.pdf
Data publikacji:
2017
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
wheat straw
biosorption
uranium
ions
biosorpcja
słoma zbożowa
uran
jony
Opis:
Basic aspects of uranium adsorption by rice husk have been investigated. The influences of various experimental parameters such as pH, time, adsorbent dosage, rice husk size, temperature and various concentrations of uranium on uptake were evaluated. The thermodynamics and kinetics of adsorption were analyzed by FT-IR and SEM. The adsorption capacity for uranium on rice husk increased upon increasing initial concentration of uranium and temperature, while decreased with the increase of the amount of rice husk. Maximum uranium adsorption was observed at pH = 3 and particle size between 120 μm and 150 μm. Adsorption equilibrium was achieved within 60 min. At 25 °C, the saturated adsorption capacity qmax was up to 15.14 mg/g when the initial concentration of uranium ranged from 10 to 400 mg/dm3. The adsorption of uranium followed Langmuir adsorption isotherm and according to quasi-second order kinetic equation. The calculated values of ΔH°, ΔS°, and ΔG° indicated that the biosorption process was endothermic and spontaneous. Based on FT-IR spectra it may be concluded that hydroxyl, carbonyl, P–O and Si=O groups are the main active sites.
Źródło:
Environment Protection Engineering; 2017, 43, 4; 41-53
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of three species commonly known as“daqingye” by internal leaf anatomy and high-performance liquid chromatography analyses
Autorzy:
Zhang, Y.
Zhu, Y.
Chen, J.
Xia, C.
Deng, J.
Li, H.
Li, Y.
Li, J.
Liu, P.
Powiązania:
https://bibliotekanauki.pl/articles/59301.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Botaniczne
Opis:
The macroscopic and microscopic morphologies and indigo and indirubin concentration of the traditional Chinese medicine herbs Isatis indigotica Fort., Polygonum tinctorium Ait., and Baphicacanthus cusia (Nees) Bremek, all commonly known as “daqingye”, were determined and compared. The morphological analyses indicated that I. indigotica has leaves with winged petioles and no glandular hairs or crystals, P. tinctorium has leaves with membranous ocrea and clusters of calcium oxalate, and B. cusia has palisade cells in the mesophyll running over the main vein and single cells containing calcium carbonate crystals. Indigo and indirubin are chemical constituents that have been previously isolated from daqingye and were selected in this study as identification markers for high-performance liquid chromatography analysis due to their pharmacological activities. The chromatographic results showed that indigo and indirubin concentration varied significantly among the three species: high concentration of both indigo and indirubin were observed in I. indigotica, the highest concentration among the three daqingye plants was found in P. tinctorium but with low levels of indirubin, and the concentration of indigo and indirubin was quite low in B. cusia. In summary, three different species commonly known as daqingye were accurately distinguished by morphological observation, internal leaf anatomy analysis, and chromatographic analysis.
Źródło:
Acta Societatis Botanicorum Poloniae; 2018, 87, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of oleic acid in different solvent media on BRL 3A cell growth and viability
Autorzy:
Liu, Runqi
Yang, Wei
Xia, Cheng
Chen, Yuanyuan
Gao, Sansi
Dong, Zhihao
Huang, Baoyin
Li, Ruirui
He, Ping
Xu, Chuang
Powiązania:
https://bibliotekanauki.pl/articles/1038377.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
oleic acid
non-alcoholic fatty liver disease
liver lipid deposition
Opis:
Oleic acid (OA) is widely used in pathology studies of hepatocellular lipid deposition. Identifying the effects of different solvents on OA-induced liver lipid deposition would be beneficial for studies on hepatocytes. We treated BRL 3A cells with OA dissolved in different solvents. After 12 h incubation, cell viability was assessed using MTT assays. Reactive oxygen species (ROS), triglyceride (TG) and total cholesterol (TC) counts, and the expression level of glucose regulated protein (GRP78), sterol regulatory element binding protein (SREBP-1C) and fatty acid synthase (FAS) were analyzed. Water, PBS and DMSO were disadvantageous to the dissolution of OA and did not cause an OA-induced response in hepatocytes. In the alcohol+OA-treated cells, the severe ER stress, oxidative stress and cellular fat deposition were significantly increased. BSA promoted cell growth and the cells treated with 1.2% BSA+OA showed a lower grade TG and endoplasmic reticulum stress compared with KOH+OA and alcohol+OA treatments. KOH had no significant influence on BRL 3A cells viability. When treated with OA dissolved in KOH, BRL 3A cells showed a typical hepatocyte damage. KOH was considered the suitable choice for an OA solvent for BRL 3A cells in hepatic lipidosis research.
Źródło:
Acta Biochimica Polonica; 2018, 65, 3; 443-447
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 27

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