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Wyświetlanie 1-15 z 184
Tytuł:
Evaluation of the gas recycling duration on the hydrocarbon recovery from gas condensate fields
Autorzy:
Matkivskyi, S.V.
Burachok, V.
Matiishyn, L.I.
Powiązania:
https://bibliotekanauki.pl/articles/24200594.pdf
Data publikacji:
2022
Wydawca:
Stowarzyszenie Komputerowej Nauki o Materiałach i Inżynierii Powierzchni w Gliwicach
Tematy:
gas condensate reservoir
condensation of heavy hydrocarbons
hydrocarbon recovery enhancement technologies
cycling process
dry gas injection period
zbiornik kondensatu gazu
kondensacja ciężkich węglowodorów
technologie zwiększania wydobycia węglowodorów
proces cykliczny
okres wtrysku suchego gazu
Opis:
Purpose: Optimization of formation pressure maintenance technologies in the development of gas condensate fields with a high initial content of condensate in the reservoir gas using numerical modelling. Design/methodology/approach: A study on the efficiency of dry gas injection for pressure maintenance in gas condensate fields was performed with the help of numerical 3D models. Key technological indicators of the reservoir development were calculated for the dry gas injection period of 12, 24, 36, 48, and 60 months. The results are presented as plots for the parameters in a study. Findings: Based on the results of the studies, it was found that the introduction of dry gas injection technology ensures that reservoir pressure is maintained at the highest level compared to the development of gas condensate reservoirs on primary depletion. Due to this, further condensate drop-out in the reservoir is slowed down, and the production of partly already condensed hydrocarbons is ensured by their evaporation into the dry gas injected from the surface. The simulation results indicate that increase in the injection duration period leads to an increase of the cumulative condensate production and hence the final hydrocarbon recovery factor. Research limitations/implications: The heterogeneity of oil and gas deposits, both in terms of area and thickness, significantly affects the efficiency of the developed hydrocarbon enhancement technologies. In order to minimize the negative impact of heterogeneity, it is necessary to conduct additional studies on the conditions of specific reservoirs or fields. Practical implications: The reservoir pressure maintenance technology implementation according to various technological schemes, as well as using various types of injection agents, will significantly intensify the development of depleted gas condensate fields with a high condensate yield. Originality/value: Statistical analysis of the simulation results identified the optimum value of the dry gas injection period into the gas condensate reservoir, which is 34.3 months for the conditions of a given reservoir in the study.
Źródło:
Archives of Materials Science and Engineering; 2022, 117, 2; 57--69
1897-2764
Pojawia się w:
Archives of Materials Science and Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:
Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:
A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7
Autorzy:
Li, Y.D.
Yu, Z.D.
Bai, C.X.
Zhang, D.
Sun, P.
Peng, M.L
Liu, H.
Wang, J.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087207.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Capsid gene
PPV7
SYBR Green I real-time PCR
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 43-49
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Ageratina adenophora on hepatic and pulmonic pathological lesions in horses
Autorzy:
Gu, X.L.
Dai, F.Y.
Xiao, X.
Li, G.Z.
Zhang, L.M.
Qu, W.J.
Powiązania:
https://bibliotekanauki.pl/articles/2087129.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Ageratina adenophora
liver
lung
horse
toxicity
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 611-613
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
17 beta-estradiol affects proliferation and apoptosis of canine bone marrow mesenchymal stem cells in vitro
Autorzy:
Zhou, Z.-H.
Gu, C.-W.
Li, J.
Huang, X.-Y.
Deng, J.-Q.
Shen, L.-H.
Cao, S.-Z.
Deng, J.-L.
Zuo, Z.-C.
Wang, Y.
Ma, X.-P.
Ren, Z.-H.
Yu, S.-M.
Powiązania:
https://bibliotekanauki.pl/articles/2087308.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
apoptosis
bone marrow mesenchymal stem cells (BMSCs)
canine
proliferation
17 beta-estradiol (E2)
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 235-245
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of organic cultivation pattern on tomato production: plant growth characteristics, quality, disease resistance, and soil physical and chemical properties
Autorzy:
Feng, X.
Xu, Y.
Liu, D.
Peng, L.
Dong, J.
Yao, S.
Feng, Y.
Feng, Z.
Li, F.
Hu, B.
Powiązania:
https://bibliotekanauki.pl/articles/12703546.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Tomato (Solanum lycopersicum L.) is one of the world’s most important cultivated vegetable. In the traditional cultivation methods, the excessive use of pesticides and fertilizers leads to an imbalance of nutrient elements in the soil, an increase in pests and diseases and a decrease in vegetable quality and yield. In the face of increasingly serious environmental and food problems, organic agriculture is considered to be an effective solution. In this experiment, the effects of organic cultivation patterns on the growth, quality, disease resistance in tomatoes, and the physical and chemical properties of soil were studied by different treatments. The results showed that the application of effective microorganisms (EM) bio-organic fertilizer in the cultivation process can significantly improve the yield, quality, and antioxidant enzyme activity of tomato. The use of straw mulching was found to significantly increase the growth, chlorophyll content, transpiration rate, and soluble sugar content of tomatoes. The application of EM bio-organic fertilizer or straw mulching significantly increased the activity of antioxidant enzyme and the expression of LeCHI gene in tomato leaves and enhanced tomato resistance to diseases. Organic production practices were found to significantly improve the soil.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 1; 71-84
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of silicon and calcium application on growth, yield and fruit quality parameters of cucumber established in a sodic soil
Autorzy:
González-Terán, G.E.
Gómez-Merino, F.C.
Trejo-Téllez, L.I.
Powiązania:
https://bibliotekanauki.pl/articles/12714269.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Soil salinity is a growing problem that affects crop quality. Cucumber is a vegetable eaten fresh, with great worldwide demand, making its chemical and physical characteristics important. In the present work, the effects of foliar application of silicon (Si: 0 and 2 mM), calcium (Ca: 0 and 2 mM), and the combination of both (Si + Ca: 0 + 0 mM, 2 + 0 mM, 0 + 2 mM and 2 + 2 mM) on dry matter of leaves and stems, yield and fruit quality of cucumbers grown in a sodic soil were studied. Treatments did not affect dry biomass, yield and product size. The obtained results show that applying foliar Ca increases total soluble solids in comparison to the control. Foliar Si application significantly increased fruit firmness in the end towards the peduncle. Moreover, foliar Ca application increased the fruit hue angle (intense green), while foliar Si application increased chroma (dark green), both significantly regarding the control. The individual applications of Si and Ca were proven to differentially improve the fruit quality parameters of cucumber in sodic soil conditions.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 3; 149-158
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression and characterisation of cucumber fruit flesh thickness-related gene CSA2M058670.1
Autorzy:
Wang, W.
Wang, S.
Guo, Y.
Li, M.
Hou, L.
Powiązania:
https://bibliotekanauki.pl/articles/12709293.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Bulked segregant analysis combined with specific length amplified fragment sequencing techniques have been applied to determine the fine genetic mapping of fruit flesh thickness-related genes in cucumber. Herein, the Csa2M058670.1 gene was subjected to real time fluorescence quantitative PCR (qRT-PCR) and sequence analysis, indicating a strong correlation with cucumber fruit flesh thickness. Expression and characterisation of the Csa2M058670.1 gene were performed based on previous studies. The results of the fluorescence-based quantitative PCR showed that Csa2M058670.1 was expressed in all organs, but levels were highest in fruit peel, fruit flesh, and female flowers. Furthermore, Csa2M058670.1 expression was induced by abiotic stresses including drought, low temperature, and high salt. Domain analysis revealed that the protein encoded by Csa2M058670.1 possesses an SET (Su(var), Enhancern of zeste CE(z), and Trithorax) domain that may control cell division and differentiation. Therefore, we speculated that Csa2M058670.1 might affect fruit flesh thickness in cucumbers by influencing cell division.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 2; 25-33
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular cloning, bioinformation analysis and expression of the strictosidine synthase in Dendrobium officinale
Autorzy:
Zhu, Y.-F.
Fan, H.-H.
Li, D.-H.
Jin, Q.
Zhang, C.-M.
Zhu, L.-Q.
Song, C.
Cai, Y.-P.
Lin, Y.
Powiązania:
https://bibliotekanauki.pl/articles/12715947.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
The enzyme strictosidine synthase (STR, EC: 4.3.3.2) plays a key role in the biosynthetic pathway of terpenoid indole alkaloid (TIA). It catalyzes the condensation of the tryptamine and secologanin to form 3α(S)-strictosidine, which is the common precursor of all TIAs. In this paper, a STR gene designated as DoSTR (GenBank: KX068707) was first cloned and characterized from Dendrobium officinale with rapid amplified cDNA ends method (RACE). DoSTR has a length of 1380bp with 1179bp open reading frame encoding 392 amino acids. BlastP analyses showed that its amino acid sequence was classified into Str_synth superfamily. qRT-PCR showed that DoSTR was expressed in all tissues tested, with a significantly higher level in flower and the lowest in stem. Four different treatments with MeJA, SA, ABA and AgNO₃, respectively, could induce the DoSTR expression to a different extent. And the effect of MeJA was the most obvious and transcript level of DoSTR induced by MeJA was 20.7 times greater than that of control at 48 hours after treatment. Furthermore, it was found that DoSTR was localized in vacuole through transient expression in tobacco. The characterization and expression of DoSTR can help in further studying the role of DoSTR in the biosynthesis of TIAs in D. officinale. This study may throw light on the alkaloid biosynthesis pathway of D. officinale.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 3; 111-124
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Parameter calibration for the discrete element simulation model of commercial organic fertilizer
Autorzy:
Xie, C.
Yang, J.
Wang, B.
Zhuo, P.
Li, C.
Wang, L.
Powiązania:
https://bibliotekanauki.pl/articles/2082983.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
commercial organic fertilizer
discrete element
repose angle
parameter calibration
parameters for the particle
model
Opis:
The parameters of a discrete element simulation model for commercial organic fertilizer particles were obtained both quickly and accurately, so that the uniformity and stability of the fertilizer discharged from the feeder may be studied using the discrete element simulation method. This study screened out the parameters which significantly influenced the repose angle of the commercial organic fertilizer using the Plackett-Burman test. Then, a model for the quadratic regression response surface of the repose angle vs the parameters was established through a Box-Behnken experiment with the measured repose angle of the fertilizer particles as the optimization index. The optimization results showed that the rolling friction coefficient and static friction coefficient between the fertilizer particles, as well as the optimal simulation parameter for the Johnson-Kendall-Roberts surface energy were 0.056, 0.355 and 0.011 J m-2, respectively. A discrete element simulation test was carried out with the optimal simulation parameters, and the repose angle error of the simulation test was found to be 0.40% relative to that of the physical test, which proved the accuracy and validity of the simulation parameters for the discrete element model of the organic fertilizer. The simulation results may provide the basic parameters for establishing a discrete element model of commercial organic fertilizer particles and investigating the performance of organic fertilizer feeders using the simulation method.
Źródło:
International Agrophysics; 2021, 35, 1; 107-117
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Response of dry matter translocation and grain yield of summer maize to biodegradable film in the North China Plain
Autorzy:
Tan, D.
Guo, L.
Liu, J.
Fan, Y.
Li, Q.
Powiązania:
https://bibliotekanauki.pl/articles/2082862.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
polyethylene film
leaf area index
dry matter
transfer
yield components
North China Plain
Opis:
In the North China Plain, it is a matter of urgency to explore the feasibility of using biodegradable film to replace polyethylene film. A field experiment was conducted by covering soils with polyethylene white film, biodegradable white film, biodegradable black film, while the control remained uncovered. This study analysed the effects of using different film types on summer maize dry matter accumulation and transfer, grain yield and yield components during the 2016 and 2017 summer maize growing seasons. Results showed that, for both growing seasons, compared with non-mulching, dry matter translocation, dry matter transfer efficiency of vegetative organs and grain yield for plants following polyethylene white film and biodegradable white film treatments were always lower. However, dry matter accumulation, dry matter translocation, dry matter transfer efficiency, grain yield, and the contribution of dry matter translocation to grain yield before flowering in biodegradable black film treatments significantly increased by 21.0, 33.3, 21.4, 12.6, and 18.5%, respectively. Only the black biodegradable film could increase grain yield as determined by the 1000 kernel mass. Results indicate that black biodegradable films are a viable alternative to polyethylene film in summer maize production in the North China Plain.
Źródło:
International Agrophysics; 2020, 34, 1; 87-94
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Machine Learning Method of PIDVCA
Autorzy:
Li, L.
Wang, X.
Chen, G.
Powiązania:
https://bibliotekanauki.pl/articles/1841618.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
learning methods
personified intelligence
collision avoidance
dynamic collision avoidance
static collision avoidance
Opis:
Building a dynamic collision knowledge base of self-learning is one of the core contents of implementing "personified intelligence" in Personifying Intelligent Decision-making for Vessel Collision Avoidance (short for PIDVCA). In the paper, the machine learning method of PIDVCA combined with offline artificial learning and online machine learning is proposed. The static collision avoidance knowledge is acquired through offline artificial learning, and the isomeric knowledge representation integration method with process knowledge as the carrier is established, and the Dynamic collision avoidance knowledge is acquired through online machine learning guided by inference engine. A large number of simulation results show that the dynamic collision avoidance knowledge base constructed by machine learning can achieve the effect of anthropomorphic intelligent collision avoidance. It is verified by examples that the machine learning method of PIDVCA can realize target perception, target cognition and finally obtain an effective collision avoidance decision-making.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2020, 14, 3; 533-540
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Thermal conductivity of a Brown Earth soil as affected by biochars derived at different temperatures: Experiment and prediction with the Campbell model
Autorzy:
Zhao, B.
Li, L.
Zhao, Y.
Zhang, X.
Powiązania:
https://bibliotekanauki.pl/articles/2082959.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
biochar
bulk density
thermal conductivity
Brown Earth soil
Campbell model
Opis:
Thermal conductivity is a significant heat transfer property of soil. However, the influence of biochar on this property is not well known. In this research, the influence of corn straw biochars prepared at 300, 500 and 700oC on the thermal conductivity of a Brown Earth (Hapli-Udic Cambisol, FAO) soil and its prediction using a Campbell model was examined. The outcomes revealed that the bulk densities of the soil markedly decreased with increases in the biochar amendment rates of 1, 3, and 5% in linear patterns. The reduction in bulk density was mainly attributed to an increase in soil porosity and organic carbon content. With increasing volumetric water contents (10, 20, 30 and 40%), the thermal conductivity of the soils significantly increased, whereas those of soils with biochar amendment were obviously less than that of the CK and the differences increased with the biochar application rates. The pyrolysis temperature of biochar exhibited a negligible effect on the bulk density and thermal conductivity of soils at large. Combining the linear reduction of bulk density with the biochar amendment rate into the Campbell model, well-fitting results for the variation inthermal conductivity versus volumetric water content were obtained and accurate values could be predicted.
Źródło:
International Agrophysics; 2020, 34, 4; 433-439
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Triplex qRT-PCR with specific probe for synchronously detecting Bovine parvovirus, bovine coronavirus, bovine parainfluenza virus and its applications
Autorzy:
Geng, J.
Niu, Y.
Wei, L.
Li, Q.
Gong, Z.
Wei, S.
Powiązania:
https://bibliotekanauki.pl/articles/2087278.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bovine parvovirus
bovine coronavirus
bovine parainfluenza virus
qRT-PCR
specificity
sensitivity
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 184

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