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Wyświetlanie 1-15 z 282
Tytuł:
A new strategy: high level expression and immunogenicity analysis of triplicate repeated multigenes in Mycoplasma hyopneumoniae
Autorzy:
Li, J.
Wang, G.
Powiązania:
https://bibliotekanauki.pl/articles/16647587.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
multi-epitope fusion protein
Mycoplasma hyopneumoniae
new generation vaccines
Opis:
Highly immunogenic nucleotide fragments from 3 genes of Mycoplasma hyopneumoniae strain 232 were selected using information software technology. After repeating each fragment three times, a total of 9 nucleotide fragments were joined together to form a new nucleotide sequence called Mhp2321092bp. Mhp2321092bp was directly synthesized and cloned into a pET100 vector and expressed in Escherichia coli. After purification, the proteins were successfully validated by SDS-PAGE and Western blotting using mouse His-tag antibody and pig anti-Mhp serum. BALB/c mice were intraperitoneally injected with purified proteins in the high-dose (100 µg), medium-dose group (50 µg) and low-dose (10 µg) groups. Mice in each group were injected on day 1, day 8 and day 15 of feeding, respectively. Serum samples were collected from all mice on the day before immunization and on day 22 after immunization. The antibody level in the mouse serum was detected using western blotting using purified expressed proteins as antigens. IL-2, TNF-α and IFN-γ were simultaneously detected in the mouse serum by ELISA. The results showed that the 60 kDa protein was successfully expressed and reacted specifically with the specific serum Mhp His-Tag mouse monoclonal antibody and pig anti-Mhp serum. From day 0 to day 22 of immunization, IFN-γ increased from 269.52 to 467.74 pg/mL, IL-2 increased from 14.03 to 145.16 pg/mL, and TNF-α increased from 6.86 to 12.37 pg/mL. The IgG antibody in mice increased significantly from 0 day to day 22 after immunization. This study suggests that the expressed recombinant protein may serve as one of the novel vaccine candidates for Mhp.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 2; 275-283
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Static instability of an inverted plate in channel flow: state-space representation and solution approximation
Autorzy:
Li, P.
Zhang, D.
Cui, J.
Yin, H.
Yang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/38697113.pdf
Data publikacji:
2023
Wydawca:
Instytut Podstawowych Problemów Techniki PAN
Tematy:
inverted cantilevered plate
static aeroelastic instability
channel flow
state-space representation
Glauert’s series
the least squares method
Opis:
Plate-like structures in channel flow are commonly found in engineering. This paper reports a theoretical study on the static aeroelastic instability of an inverted cantilevered plate in an inviscid channel flow through the state space. This study begins with the kernel function of the flow potential determined in the Fourier domain with the help of the mirror image method. Then, the instability equation is derived from the operator theory and transformed in the state space. Finally, with Glauert’s expansion, model functions, and error functions, the instability problem of such a plate has been modeled as a mathematical function approximation problem and solved by the least squares method. The derived instability equation is considered at the continuum level of description, and no approximation appears at the first equation level. The convergence and reliability of the proposed modeling and its solutions approximation are entirely tested, and it can successfully predict the instability boundary, behavior, and the channel effect. Numerical results show that the decreased channel height and asymmetric plate placement in the channel significantly decrease the critical flow velocity. The plate instability modes are close to the plate’s first natural ones and not sensitive to the channel parameters. This conclusion allows further theoretical exploration of a semi-analytical approximation of the instability boundary from the obtained instability equation. The current modeling strategy in a continuum sense may provide a new idea and essential reference for other instability problems.
Źródło:
Archives of Mechanics; 2023, 75, 6; 695-727
0373-2029
Pojawia się w:
Archives of Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of organic supplementation to reduced rates of chemical fertilization on soil fertility of Zanthoxylum armatum
Autorzy:
Saba, T.
Liu, W.
Wang, J.
Saleem, F.
Kang, X.
Hui, W.
Gong, W.
Li, H.
Powiązania:
https://bibliotekanauki.pl/articles/2130175.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
fertilization
soil fertility
nutrient availability
organic manure
culturable microbes
Opis:
Soil acidification, fertility depletion, food insecurity, and environmental pollution all are consequences of the extensive use of chemical fertilizers (CF) in intensively managed plantations. Recent studies have shown that incorporating organic manure (OM) to partially replace CF can help to maintain productivity and soil health. There are no reports on integrated fertilizer management of Zanthoxylum armatum, for optimal ecological services and economic profitability. A pot experiment with Z. armatum was performed to investigate the possibility of incorporating OM in reducing CF use, enhancing soil fertility, and increasing plant growth. For this, seven treatments were designed: NPK, PK, NP, NK, OM, MNPK (50%NPK+50%OM), and control (CK, no fertilizer application). The results showed that NPK application significantly (P < 0.05) increased plant growth, soil nitrate-N, ammonium-N and available K compared to CK. However conventional CF application induced soil acidification. OM application raised the soil pH from 5.50 (CK) to 6.58 and significantly enhanced soil fertility by increasing alkali-hydrolysable N (2.3 times), ammonium-N (1.4 times), available P (4.3 times), the activity of invertase (1.5 times), catalase (2.8 times), acid phosphatase (1.5 times), and count of soil culturable microbes (2.9 times) compared to CK. Conversely, sole OM application did not result in optimum plant growth. Integrating OM with NPK (MNPK), on the other hand, not only provided the highest growth comprehensive value (0.84), but also substantially higher soil fertility comprehensive value (0.71) than traditional CF treatments. Correlation analysis also indicated a positive and significant correlation between soil microbes, enzyme activities and available nutrients. Therefore, OM+NPK could be an optimum measure to get maximum benefits regarding soil fertility, growth of Z. armatum, fertilizer savings and sustainable agroecology.
Źródło:
Dendrobiology; 2022, 87; 123-136
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:
Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:
https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:
A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7
Autorzy:
Li, Y.D.
Yu, Z.D.
Bai, C.X.
Zhang, D.
Sun, P.
Peng, M.L
Liu, H.
Wang, J.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087207.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Capsid gene
PPV7
SYBR Green I real-time PCR
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 43-49
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Ageratina adenophora on hepatic and pulmonic pathological lesions in horses
Autorzy:
Gu, X.L.
Dai, F.Y.
Xiao, X.
Li, G.Z.
Zhang, L.M.
Qu, W.J.
Powiązania:
https://bibliotekanauki.pl/articles/2087129.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Ageratina adenophora
liver
lung
horse
toxicity
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 611-613
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Organ-level evaluation of the carbon starvation hypothesis in deciduous broad-leaved Catalpa bungei plants undergoing drought-induced mortality
Autorzy:
Qiu, Q.
Wang, J.
Su, Y.
Li, J.
Ma, J.
He, Q.
Powiązania:
https://bibliotekanauki.pl/articles/2078283.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Carbon starvation
Catalpa bungei
Drought
Non-structural carbohydrate (NSC)
Plant mortal-
ity
Opis:
The carbon starvation hypothesis (CSH) is one of the current leading hypotheses regarding the mechanism of plant death, although it has not been sufficiently validated due to a lack of evidence. To help verify the role of carbon starvation in plant mortality, we measured the non-structural carbohydrate (NSC) concentration ([NSC]) in different organs (roots, stems, and leaves) of plants of Catlapa bungei clone 9-1 after the cessation of photosynthesis and death due to drought and in well-watered controls. Drought in- duced the loss of NSC reserves, which was observed in all organs at death, and carbon starvation appeared to begin after the cessation of photosynthesis. The [NSC] dynamics, occurrence time of carbon starvation, and survival time varied among the organs, and even within the same organs at different stem heights. Overall, our findings are compatible with the CSH in that carbon starvation occurs in roots, stems, and leaves and concurs with plant death during prolonged drought.
Źródło:
Dendrobiology; 2018, 80; 48-60
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
17 beta-estradiol affects proliferation and apoptosis of canine bone marrow mesenchymal stem cells in vitro
Autorzy:
Zhou, Z.-H.
Gu, C.-W.
Li, J.
Huang, X.-Y.
Deng, J.-Q.
Shen, L.-H.
Cao, S.-Z.
Deng, J.-L.
Zuo, Z.-C.
Wang, Y.
Ma, X.-P.
Ren, Z.-H.
Yu, S.-M.
Powiązania:
https://bibliotekanauki.pl/articles/2087308.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
apoptosis
bone marrow mesenchymal stem cells (BMSCs)
canine
proliferation
17 beta-estradiol (E2)
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 235-245
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of organic cultivation pattern on tomato production: plant growth characteristics, quality, disease resistance, and soil physical and chemical properties
Autorzy:
Feng, X.
Xu, Y.
Liu, D.
Peng, L.
Dong, J.
Yao, S.
Feng, Y.
Feng, Z.
Li, F.
Hu, B.
Powiązania:
https://bibliotekanauki.pl/articles/12703546.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Tomato (Solanum lycopersicum L.) is one of the world’s most important cultivated vegetable. In the traditional cultivation methods, the excessive use of pesticides and fertilizers leads to an imbalance of nutrient elements in the soil, an increase in pests and diseases and a decrease in vegetable quality and yield. In the face of increasingly serious environmental and food problems, organic agriculture is considered to be an effective solution. In this experiment, the effects of organic cultivation patterns on the growth, quality, disease resistance in tomatoes, and the physical and chemical properties of soil were studied by different treatments. The results showed that the application of effective microorganisms (EM) bio-organic fertilizer in the cultivation process can significantly improve the yield, quality, and antioxidant enzyme activity of tomato. The use of straw mulching was found to significantly increase the growth, chlorophyll content, transpiration rate, and soluble sugar content of tomatoes. The application of EM bio-organic fertilizer or straw mulching significantly increased the activity of antioxidant enzyme and the expression of LeCHI gene in tomato leaves and enhanced tomato resistance to diseases. Organic production practices were found to significantly improve the soil.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 1; 71-84
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of tillage alteration on soil water content, maize crop water potential and grain yield under subtropical humid climate conditions
Autorzy:
Chen, J.
He, Y.
Li, P.
Powiązania:
https://bibliotekanauki.pl/articles/2082993.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
soil penetration resistance
occasional deep tillage
no-till
seasonal drought
Opis:
Seasonal drought stress is common in farmland even under humid climate conditions. Low soil water content and high penetration resistance in clayey soil are both factors that limit crop growth, which is significantly affected by tillage. In a twoyear (2014-2015) field experiment conducted in Hubei, China, the effects of conventional tillage, along with occasional deep tillage and no-till, on the soil water content and penetration resistance values of red soil and on the crop water potentials of the maize crop (Zea mays L.) were tested. Compared to conventional tillage, deep tillage reduced the difference in the soil water characteristic curve between 0-40 cm soil layers, resulting in a more loose and homogeneous topsoil. The deep tillage significantly (p<0.05) decreased soil penetration resistance, increased soil-available water content and soil water content during the dry period, promoted an increase in maize root density by 11.4~31.6%, and increased the water potential of the maize root and leaf during most growth stages. In contrast, the effect of no-till was opposite to that of deep tillage, reducing maize grain yield by 25.3~26.3%. The results confirmed that no-till is not appropriate for the clayey red soil but rather that tillage is needed. This suggests that occasional deep tillage is helpful in mitigating seasonal crop drought stress under the conditions of a humid climate.
Źródło:
International Agrophysics; 2021, 35, 1; 1-9
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Parameter calibration for the discrete element simulation model of commercial organic fertilizer
Autorzy:
Xie, C.
Yang, J.
Wang, B.
Zhuo, P.
Li, C.
Wang, L.
Powiązania:
https://bibliotekanauki.pl/articles/2082983.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
commercial organic fertilizer
discrete element
repose angle
parameter calibration
parameters for the particle
model
Opis:
The parameters of a discrete element simulation model for commercial organic fertilizer particles were obtained both quickly and accurately, so that the uniformity and stability of the fertilizer discharged from the feeder may be studied using the discrete element simulation method. This study screened out the parameters which significantly influenced the repose angle of the commercial organic fertilizer using the Plackett-Burman test. Then, a model for the quadratic regression response surface of the repose angle vs the parameters was established through a Box-Behnken experiment with the measured repose angle of the fertilizer particles as the optimization index. The optimization results showed that the rolling friction coefficient and static friction coefficient between the fertilizer particles, as well as the optimal simulation parameter for the Johnson-Kendall-Roberts surface energy were 0.056, 0.355 and 0.011 J m-2, respectively. A discrete element simulation test was carried out with the optimal simulation parameters, and the repose angle error of the simulation test was found to be 0.40% relative to that of the physical test, which proved the accuracy and validity of the simulation parameters for the discrete element model of the organic fertilizer. The simulation results may provide the basic parameters for establishing a discrete element model of commercial organic fertilizer particles and investigating the performance of organic fertilizer feeders using the simulation method.
Źródło:
International Agrophysics; 2021, 35, 1; 107-117
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Response of dry matter translocation and grain yield of summer maize to biodegradable film in the North China Plain
Autorzy:
Tan, D.
Guo, L.
Liu, J.
Fan, Y.
Li, Q.
Powiązania:
https://bibliotekanauki.pl/articles/2082862.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
polyethylene film
leaf area index
dry matter
transfer
yield components
North China Plain
Opis:
In the North China Plain, it is a matter of urgency to explore the feasibility of using biodegradable film to replace polyethylene film. A field experiment was conducted by covering soils with polyethylene white film, biodegradable white film, biodegradable black film, while the control remained uncovered. This study analysed the effects of using different film types on summer maize dry matter accumulation and transfer, grain yield and yield components during the 2016 and 2017 summer maize growing seasons. Results showed that, for both growing seasons, compared with non-mulching, dry matter translocation, dry matter transfer efficiency of vegetative organs and grain yield for plants following polyethylene white film and biodegradable white film treatments were always lower. However, dry matter accumulation, dry matter translocation, dry matter transfer efficiency, grain yield, and the contribution of dry matter translocation to grain yield before flowering in biodegradable black film treatments significantly increased by 21.0, 33.3, 21.4, 12.6, and 18.5%, respectively. Only the black biodegradable film could increase grain yield as determined by the 1000 kernel mass. Results indicate that black biodegradable films are a viable alternative to polyethylene film in summer maize production in the North China Plain.
Źródło:
International Agrophysics; 2020, 34, 1; 87-94
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Simplified analyses of stress induced anisotropy in remolded soft clay under undrained conditions
Autorzy:
Lin, P.
Li, Z.-x.
Garg, A.
Yadav, J. S.
Powiązania:
https://bibliotekanauki.pl/articles/1818489.pdf
Data publikacji:
2020
Wydawca:
Stowarzyszenie Komputerowej Nauki o Materiałach i Inżynierii Powierzchni w Gliwicach
Tematy:
stress induced anisotropy
Shantou soft clay
true triaxial experiment
anizotropia wywołana naprężeniem
miękka glinka Shantou
prawdziwy eksperyment trójosiowy
Opis:
Purpose: The soil’s anisotropy induced by stress (i.e. stress induced anisotropy) has an important effect on the behavior of soil. This paper focuses on analyzing the anisotropy of remolded Shantou soft clay under compression stress path. Design/methodology/approach: Experiments were executed by using three axle experimental instruments. The data obtained from the plain strain tests were analyzed and the relationship between stress and strain was calculated by using the modified Duncan- Chang and Lade-Duncan models. The models were modified under the condition of plain strain and cohesion. Findings: It was concluded that in complex stress path conditions, the conventional triaxial tests may not fully reflect the actual stress of soil and its response in the Duncan-Chang and Lade-Duncan models. Research limitations/implications: The formulation of Mohr-Coulomb failure criterion in the plasticity framework is quite diffcult. As a result, dilatancy cannot be described. The properties of soil in unload or drained conditions remain to be part of further investigated. Practical implications: Based upon the two stiffness parameters, the modified Duncan- Chang model has captured the soil behaviour in a very conformable way and is recommened for practical modeling. These constitutive models of soil are widely used in the numerical analyses of soil structure such as embankments. Originality/value: Duncan-Chang and Lade-Duncan models widely used in engineering practices are modes based on conventional triaxial cases. Both models have have some inherent limitations to represent the stress-strain characteristics of soils, such as shear-induced dilatancy and stress path dependency and required corrections. In this investigation, the tests are carried out in undrained conditions. It is related to the properties of soil in load conditions.
Źródło:
Archives of Materials Science and Engineering; 2020, 105, 2; 56--64
1897-2764
Pojawia się w:
Archives of Materials Science and Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 282

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