- Tytuł:
- Dengue virus (NS2B/NS3 protease) protein engineering. Part I: An automated design for hotspots stability and site-specific mutations by using HotSpot Wizard 3.0 tool
- Autorzy:
-
Lahiri, Madhumita
Ghosh, Ipsita
Talukdar, Partha
Talapatra, Soumendra Nath - Powiązania:
- https://bibliotekanauki.pl/articles/1062840.pdf
- Data publikacji:
- 2019
- Wydawca:
- Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
- Tematy:
-
HotSpot Wizard
NS2B/NS3 protease
Non-structural protein
computational tool
protein engineering - Opis:
- The non-structural dengue virus (DNV) protein, DNV-2 NS2B/NS3 protease is a combination of two proteins as 2B and 3 and these two proteins in complex replicate faster during dengue fever. The objective of the present study was to detect hot spots and design of smart libraries for engineering protein stability, substrate specificity, tunnels and cavities as well as suitable mutability position of studied protein by using an online tool, HotSpot Wizard (version 3.0). The prediction results were obtained in output interface for functional hot spots, stability hot spots (structural flexibility), correlated hot spots and stability hot spots (sequence consensus) from the sequence string. It is concluded that the prediction of pocket and mutability of this protein can easily be identified the structural alternation especially in disease diagnosis and space for ligand binding site in pocket for the potential of new drug design. Moreover, this computational prediction is suggested to compare with experimental hotspots for studied protein in relation to therapeutic efficacies, which are lacking to prevent viral infection.
- Źródło:
-
World Scientific News; 2019, 127, 3; 177-190
2392-2192 - Pojawia się w:
- World Scientific News
- Dostawca treści:
- Biblioteka Nauki
Artykuł